##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527701_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1336050
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96269525841099	33.0	31.0	34.0	30.0	34.0
2	32.121062834474756	33.0	31.0	34.0	30.0	34.0
3	32.56744133827327	34.0	31.0	34.0	31.0	34.0
4	35.83446353055649	37.0	35.0	37.0	35.0	37.0
5	36.022856180532166	37.0	35.0	37.0	35.0	37.0
6	35.56451105871786	37.0	35.0	37.0	33.0	37.0
7	36.362652595337	37.0	35.0	37.0	35.0	37.0
8	36.601030650050525	37.0	37.0	37.0	35.0	37.0
9	38.647545376295795	39.0	39.0	39.0	38.0	39.0
10	37.91416339208862	39.0	38.0	39.0	35.0	39.0
11	37.553221810560984	39.0	37.0	39.0	35.0	39.0
12	37.25039556902811	39.0	37.0	39.0	34.0	39.0
13	37.19550241383182	39.0	37.0	39.0	34.0	39.0
14	37.955384154784625	40.0	38.0	40.0	34.0	40.0
15	38.07069570749598	40.0	38.0	40.0	34.0	40.0
16	38.08134276411811	40.0	38.0	40.0	34.0	40.0
17	38.057269563264846	40.0	38.0	40.0	34.0	40.0
18	38.031791474869955	40.0	38.0	40.0	34.0	40.0
19	38.0210313985255	40.0	38.0	40.0	34.0	40.0
20	37.988546835822014	40.0	38.0	40.0	34.0	40.0
21	37.94944350885071	40.0	38.0	40.0	34.0	40.0
22	37.935717226151716	40.0	38.0	40.0	34.0	40.0
23	37.86892406721305	40.0	37.0	40.0	34.0	40.0
24	37.732024250589426	40.0	37.0	40.0	34.0	40.0
25	37.50691441188578	40.0	36.0	40.0	33.0	40.0
26	37.312888739193895	40.0	36.0	40.0	33.0	40.0
27	37.146089592455375	39.0	35.0	40.0	33.0	40.0
28	37.03783241645148	39.0	35.0	40.0	32.0	40.0
29	36.865819392986786	39.0	35.0	40.0	32.0	40.0
30	36.6790105160735	39.0	35.0	40.0	31.0	40.0
31	36.54054189588713	39.0	35.0	40.0	31.0	40.0
32	36.35838104861345	39.0	35.0	40.0	31.0	40.0
33	36.16304479622769	38.0	35.0	40.0	30.0	40.0
34	35.94922645110587	38.0	35.0	40.0	30.0	40.0
35	35.79020770180757	38.0	35.0	40.0	29.0	40.0
36	35.5966692863291	38.0	35.0	40.0	28.0	40.0
37	35.24807379963325	38.0	35.0	40.0	26.0	40.0
38	35.01588563302272	38.0	34.0	40.0	26.0	40.0
39	34.72148272893978	38.0	34.0	40.0	24.0	40.0
40	34.39199805396505	38.0	34.0	40.0	23.0	40.0
41	34.15455708992927	37.0	33.0	40.0	22.0	40.0
42	33.976510609632875	37.0	33.0	40.0	22.0	40.0
43	33.758600351783244	37.0	33.0	40.0	21.0	40.0
44	33.53978818158003	36.0	33.0	40.0	21.0	40.0
45	33.35292316904307	36.0	33.0	40.0	20.0	40.0
46	33.168876913289175	35.0	33.0	39.0	20.0	40.0
47	32.91916320496988	35.0	32.0	39.0	20.0	40.0
48	32.711379813629726	35.0	32.0	39.0	18.0	40.0
49	32.94784551476367	35.0	33.0	39.0	20.0	40.0
50	32.905249055050334	35.0	33.0	39.0	18.0	40.0
51	32.80197073462819	35.0	33.0	39.0	18.0	40.0
52	32.277446203360654	35.0	32.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	0.0
13	3.0
14	4.0
15	22.0
16	59.0
17	149.0
18	360.0
19	805.0
20	1499.0
21	2554.0
22	4227.0
23	6698.0
24	9751.0
25	13045.0
26	17599.0
27	23558.0
28	28171.0
29	29885.0
30	30011.0
31	33560.0
32	41885.0
33	53152.0
34	84419.0
35	100088.0
36	150080.0
37	228941.0
38	348668.0
39	126853.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.1298229856667	0.6048426331349874	0.20927360502975187	0.056060776168556564
8	98.5135286853037	1.0706186145728078	0.18075670820702816	0.23509599191647018
9	97.30406796152839	1.514164889038584	0.6167433853523446	0.5650237640806857
10	58.706485535720965	26.34145428689046	5.791624564948917	9.160435612439654
11	31.596796527076084	30.003517832416453	18.05972830358145	20.339957336926012
12	28.445642004416	22.313611017551736	26.16743385352345	23.07331312450881
13	23.125032745780473	23.00385464615845	27.65757269563265	26.21353991242843
14	23.057969387373227	25.43452715092998	30.50589424048501	21.00160922121178
15	25.695220987238503	23.09374649152352	28.1368212267505	23.074211294487483
16	26.818981325549196	24.81793346057408	24.818981325549196	23.544103888327534
17	29.738407993712812	24.548332771977098	21.756146850791513	23.95711238351858
18	27.170839414692566	25.33580330077467	22.31503312001796	25.178324164514805
19	28.980053141723737	25.665731072938886	21.394334044384568	23.95988174095281
20	26.52483065753527	27.11874555592979	22.3439242543318	24.012499532203137
21	26.57116125893492	25.836158826391227	24.852213614759926	22.740466299913926
22	27.50480895176079	24.552449384379326	23.443434003218442	24.499307660641442
23	27.247558100370494	26.527824557464168	21.662138393024215	24.562478949141127
24	28.086673402941507	26.080461060589048	21.911455409602933	23.921410126866512
25	27.325249803525313	26.82549305789454	20.384566445866547	25.4646906927136
26	28.719958085400993	25.65001309831219	20.755660342053066	24.874368474233748
27	26.652894726993747	25.15212754013697	21.491860334568315	26.703117398300964
28	28.787395681299355	26.501927323079226	19.757494105759516	24.953182889861907
29	30.045881516410315	26.332921672093114	20.336888589498898	23.28430822199768
30	28.5761011938176	25.74222521612215	21.431757793495755	24.2499157965645
31	29.736087721267918	27.00437857864601	19.39822611429213	23.861307585793945
32	31.102279106320875	25.115003181018675	20.521761910108154	23.2609558025523
33	30.900639946109802	25.084016316754614	20.601624190711426	23.41371954642416
34	27.922457991841622	28.21690804984843	22.28112720332323	21.579506754986717
35	29.414842258897494	28.38995546573856	21.62359193143969	20.571610343924256
36	29.57935705999027	28.18277759065903	20.8842483439991	21.353617005351598
37	28.973092324389054	28.06264735601213	21.205269263874854	21.75899105572396
38	30.680663148834252	26.801766400958048	20.245350099172935	22.272220351034765
39	28.80498484338161	25.870813218068182	19.473747239998502	25.8504546985517
40	29.057969387373227	26.557688709254894	20.86740765689907	23.51693424647281
41	27.788930055012912	26.448561056846675	21.863478163242394	23.899030724898022
42	26.16743385352345	26.331948654616223	22.278357845889	25.222259645971334
43	28.53358781482729	25.185434676845926	22.328430822199767	23.952546686127015
44	27.289846936866134	25.27607499719322	21.40196848920325	26.0321095767374
45	27.03970659780697	25.65547696568242	22.12050447213802	25.18431196437259
46	26.758429699487294	26.92369297556229	20.886942853935107	25.430934471015306
47	25.78698402005913	26.890909771340894	23.242543317989597	24.079562890610383
48	26.10822948242955	27.357284532764492	22.44174993450844	24.09273605029752
49	27.44231129074511	27.673964297743346	21.321208038621307	23.562516372890236
50	24.720706560383217	28.170652295947008	22.16608659855544	24.94255454511433
51	24.815313798136298	29.0224168257176	22.25500542644362	23.907263949702482
52	26.383817970884323	27.679802402604693	22.439953594551103	23.49642603195988
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	38.0
4	76.0
5	110.5
6	145.0
7	177.5
8	210.0
9	2610.5
10	5011.0
11	4715.5
12	4420.0
13	4510.5
14	4385.5
15	4170.0
16	4194.5
17	4219.0
18	4573.0
19	4927.0
20	5414.5
21	5902.0
22	7429.5
23	8957.0
24	10759.5
25	12562.0
26	13868.0
27	15174.0
28	16592.5
29	18011.0
30	19874.0
31	21737.0
32	22039.5
33	22342.0
34	24675.0
35	27008.0
36	27028.0
37	27048.0
38	29043.0
39	32714.0
40	34390.0
41	36911.5
42	39433.0
43	41243.0
44	43053.0
45	47229.5
46	51406.0
47	50650.5
48	49895.0
49	53453.0
50	57011.0
51	65258.5
52	73506.0
53	77617.5
54	81729.0
55	90655.5
56	99582.0
57	101080.0
58	102578.0
59	100768.5
60	98959.0
61	97518.5
62	96078.0
63	86194.0
64	71449.0
65	66588.0
66	58697.0
67	50806.0
68	43304.5
69	35803.0
70	32086.5
71	28370.0
72	22862.5
73	17355.0
74	12745.0
75	8135.0
76	6293.5
77	4452.0
78	3189.5
79	1927.0
80	1439.0
81	951.0
82	536.5
83	122.0
84	75.5
85	29.0
86	23.5
87	18.0
88	12.0
89	3.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1336050.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.40072805677837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.89894458958884	12.611986780671147
2	8.648667217416717	2.836888781728513
3	3.6024306131257964	1.7724745448786863
4	1.8196846189533706	1.1937661033822642
5	1.0612718411901358	0.8702815430837951
6	0.6376242535273888	0.6274501190705406
7	0.4666394732379248	0.5357258970793459
8	0.3574595187677673	0.4690077086893612
9	0.296999885089637	0.4383912913424605
>10	3.0634028954345345	13.179407563619877
>50	1.9726134974023535	23.104433685385978
>100	1.110849645262914	31.67936375735456
>500	0.045620108635027266	5.135440901200136
>1k	0.017335641281310363	4.973006030845874
>5k	4.562010863502727E-4	0.5723752916674831
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	7650	0.5725833614011452	No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4733	0.3542532090864863	No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	4567	0.3418285243815726	No Hit
TAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3727	0.2789566258747801	No Hit
TAGAGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	2910	0.2178062198271023	No Hit
TAGAGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	2672	0.19999251525017778	No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2624	0.1963998353355039	No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGAAG	2606	0.19505258036750123	No Hit
TAGAGAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	2453	0.1836009131394783	No Hit
TAGAGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2280	0.17065229594700798	No Hit
TAGAGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1978	0.14804835148385165	No Hit
TAGAGAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA	1875	0.14033905916694733	No Hit
TAGAGAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	1841	0.13779424422738668	No Hit
TAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1784	0.1335279368287115	No Hit
TAGAGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1652	0.12364806706335842	No Hit
TAGAGAAGGGGAGTTTTCCTGCAAGTTGAGTAGCGCCAAGGCCGCTGCAGAA	1580	0.11825904719134762	No Hit
TAGAGAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1452	0.10867856741888403	No Hit
TAGAGAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	1421	0.10635829497399049	Illumina Single End Adapter 2 (100% over 34bp)
TAGAGAGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTC	1418	0.10613375247932337	No Hit
TAGAGAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	1367	0.10231653006998241	Illumina Single End Adapter 2 (100% over 34bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.09782568017664009	0.0	0.0	0.0	0.0
5	0.09782568017664009	0.0	0.0	0.0	0.0
6	0.2854683582201265	0.0	0.0	0.0	0.0
7	0.2886119531454661	0.0	0.0	0.0	0.0
8	0.3845664458665469	0.0	0.0	0.0	0.0
9	0.5322405598592868	0.0	0.0	0.0	0.0
10	0.6906178660978257	0.0	0.0	0.0	0.0
11	0.9543804498334643	0.0	0.0	0.0	0.0
12	1.0364881553834062	0.0	0.0	0.0	0.0
13	1.1047490737622094	0.0	0.0	0.0	0.0
14	1.1745069421054601	0.0	0.0	0.0	0.0
15	1.2495041353242768	0.0	0.0	0.0	0.0
16	1.313947831293739	0.0	0.0	0.0	0.0
17	1.3712061674338536	0.0	0.0	0.0	0.0
18	1.4283896560757456	0.0	0.0	0.0	0.0
19	1.4955278619812133	0.0	0.0	0.0	0.0
20	1.560270947943565	0.0	0.0	0.0	0.0
21	1.6179035215747914	0.0	0.0	0.0	0.0
22	1.685415964971371	0.0	0.0	0.0	0.0
23	1.7563713932861793	0.0	0.0	0.0	0.0
24	1.8312188915085512	0.0	0.0	0.0	0.0
25	1.9067400172149247	0.0	0.0	0.0	0.0
26	1.98547958534486	0.0	0.0	0.0	0.0
27	2.051569926275214	0.0	0.0	0.0	0.0
28	2.128588001946035	0.0	0.0	0.0	0.0
29	2.17671494330302	0.0	0.0	0.0	0.0
30	2.2365929418809176	0.0	0.0	0.0	0.0
31	2.2779836083978893	0.0	0.0	0.0	0.0
32	2.324838142285094	0.0	0.0	0.0	0.0
33	2.3675760637700685	0.0	0.0	0.0	0.0
34	2.4118109352194903	0.0	0.0	0.0	0.0
35	2.476628868680064	0.0	0.0	0.0	0.0
36	2.518094382695258	0.0	0.0	0.0	0.0
37	2.5619550166535685	0.0	0.0	0.0	0.0
38	2.607836533063882	0.0	0.0	0.0	0.0
39	2.652071404513304	0.0	0.0	0.0	0.0
40	2.694734478500056	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAT	25	3.4174554E-5	46.0	38
AACGTTG	20	6.311825E-4	46.0	38
AACGTCG	25	3.4174554E-5	46.0	17
AACGTAC	25	3.4174554E-5	46.0	44
CGAAATC	20	6.311825E-4	46.0	20
TAGGTTA	20	6.311825E-4	46.0	20
GTTAGCA	20	6.311825E-4	46.0	32
TAACGGC	30	1.861632E-6	46.0	24
GCGTTAG	45	3.110472E-10	46.0	23
TCGATAT	30	1.861632E-6	46.0	20
AGTATCG	20	6.311825E-4	46.0	17
TTATACG	20	6.311825E-4	46.0	31
TACGCTA	30	1.861632E-6	46.0	45
TGTTACG	20	6.311825E-4	46.0	15
CGTACGC	40	5.6134013E-9	46.0	43
CGATGTA	25	3.4174554E-5	46.0	27
CACGTTA	20	6.311825E-4	46.0	42
CGACTTA	55	1.8189894E-12	46.0	21
TATACGC	20	6.311825E-4	46.0	32
TAAGCGC	20	6.311825E-4	46.0	38
>>END_MODULE