FastQCFastQC Report
Fri 17 Jun 2016
SRR1527700_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527700_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345312
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA72450.5385367855188983No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC44580.3313729454580053No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT42980.31947979353488265No Hit
TAGAGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34620.25733807473656667No Hit
TAGAGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA29260.21749601579410574No Hit
TAGAGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA24720.1837491972122452No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT24580.18270854641897197No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGAGAGGGAAG23760.17661330605837158No Hit
TAGAGAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT22190.16494315073380747No Hit
TAGAGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC21500.15981422896696082No Hit
TAGAGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC19090.1419001688827573No Hit
TAGAGAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA17400.129338027163959No Hit
TAGAGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG16670.12391177659903428No Hit
TAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16280.12101282081777313No Hit
TAGAGAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA16170.12019516662305846No Hit
TAGAGAAGGGGAGTTTTCCTGCAAGTTGAGTAGCGCCAAGGCCGCTGCAGAA16110.11974917342594134No Hit
TAGAGAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC14170.10532872671915512No Hit
TAGAGAGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTC14100.1048084013225185No Hit
TAGAGAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA13930.10354475393068671Illumina Single End Adapter 2 (100% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAT405.6134013E-946.00000438
ACGTTAT206.311839E-446.00000417
TACGCTA206.311839E-446.00000445
TAACACG206.311839E-446.00000430
TCCCGTA206.311839E-446.00000417
CATAATC206.311839E-446.00000424
ACGTATT405.6134013E-946.00000420
TTCGATA206.311839E-446.00000434
TCGGTAT206.311839E-446.00000436
TACGGTT253.4174685E-546.024
CCGTCGA253.4174685E-546.044
CGTACTC301.8616411E-646.035
TTTATCG253.4174685E-546.011
TAGATCC253.4174685E-546.011
CGATATA253.4174685E-546.010
CGATACA301.8616411E-646.041
TACGTTC501.6370905E-1146.026
GTACGTT253.4174685E-546.010
AACGCGT351.01974365E-745.99999620
CGTCAAA351.01974365E-745.99999641
Produced by FastQC (version 0.11.5)