FastQCFastQC Report
Fri 17 Jun 2016
SRR1527690_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527690_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1793418
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC227171.266687409181797No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG169600.945680259705211No Hit
AACCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT71310.39762063278053417No Hit
AACCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT69570.3879184886066717No Hit
AACCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC63860.356079843070606No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG60770.3388501732446089No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT52350.29190071695499875No Hit
AACCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG44550.24840834652044308No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGAGGGTAAGAGTACCCCACCT40800.22749855304229133No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC36360.20274135756415962No Hit
AACCTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT31300.1745270762309735No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC31270.17435979788314826No Hit
AACCTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT28440.15857987373830304No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT28410.15841259539047786No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC26790.1493795646079163No Hit
AACCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA26680.1487662106658905No Hit
AACCTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG23970.13365539991234612No Hit
AACCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA23350.13019831405729174No Hit
AACCTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC21950.12239199115878172No Hit
AACCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG21350.11904642420227744No Hit
AACCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG21120.11776395686895079No Hit
AACCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC21010.11715060292692502No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGC19910.11101706350666715No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA19050.106221750869011No Hit
AACCTAGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGC18840.10505080243423451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGAC207.8576646E-444.042
CGCGAAT254.443841E-544.040
ACGATAG254.443841E-544.037
TACTCGA254.443841E-544.044
CCGCGTA254.443841E-544.041
ACGGTAT302.5285135E-644.012
TAGGGAT182900.042.712965
CTAGGGA531200.042.5877274
AACCTAG1843300.042.5367551
ACCTAGG1832450.042.530492
CCTAGGG1811900.042.510183
TAGGGGA276650.042.43345
CTAGGGG1134600.042.427464
TAGGGGG525550.042.3046345
TAGGGAG174550.042.2858775
TAGGGGT147250.042.177255
TAGGGAA92600.042.123115
TAGGGGC189050.042.0798765
TAGGGCA39150.041.8646245
AGGGGGG284000.041.621836
Produced by FastQC (version 0.11.5)