##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527690_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1793418
Sequences flagged as poor quality	0
Sequence length	50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.95059991591475	33.0	31.0	34.0	3.0	34.0
2	30.47777930186939	34.0	31.0	34.0	16.0	34.0
3	32.29583343091237	34.0	31.0	34.0	28.0	34.0
4	36.31851916284993	37.0	35.0	37.0	35.0	37.0
5	36.338837348571275	37.0	37.0	37.0	35.0	37.0
6	36.42237280990823	37.0	37.0	37.0	35.0	37.0
7	36.59670863122819	37.0	37.0	37.0	35.0	37.0
8	36.70828663479457	37.0	37.0	37.0	35.0	37.0
9	38.781774800966645	39.0	39.0	39.0	39.0	39.0
10	38.37446986703602	39.0	39.0	39.0	37.0	39.0
11	38.192402440479576	39.0	39.0	39.0	37.0	39.0
12	37.52580714590798	39.0	37.0	39.0	35.0	39.0
13	37.391434121883464	39.0	37.0	39.0	35.0	39.0
14	38.11830538112141	40.0	38.0	40.0	35.0	40.0
15	38.134669106700166	40.0	37.0	40.0	35.0	40.0
16	38.17267363213707	40.0	37.0	40.0	35.0	40.0
17	38.02841166978362	40.0	37.0	40.0	34.0	40.0
18	37.98490368670327	40.0	37.0	40.0	34.0	40.0
19	37.9582495547608	40.0	37.0	40.0	34.0	40.0
20	37.89195212716723	40.0	37.0	40.0	34.0	40.0
21	37.88748412249682	40.0	37.0	40.0	34.0	40.0
22	37.938149946080614	40.0	37.0	40.0	34.0	40.0
23	37.91186996004278	40.0	37.0	40.0	34.0	40.0
24	37.90388966766253	40.0	37.0	40.0	34.0	40.0
25	37.897819694014444	40.0	36.0	40.0	34.0	40.0
26	37.78722584472778	40.0	36.0	40.0	34.0	40.0
27	37.72034071253885	40.0	36.0	40.0	34.0	40.0
28	37.54978147871829	40.0	35.0	40.0	34.0	40.0
29	37.54142871321689	40.0	35.0	40.0	34.0	40.0
30	37.67574486260314	40.0	36.0	40.0	34.0	40.0
31	37.71852518487045	40.0	36.0	40.0	34.0	40.0
32	37.69295724699986	40.0	35.0	40.0	34.0	40.0
33	37.596423700442394	40.0	35.0	40.0	34.0	40.0
34	37.197155375935786	40.0	35.0	40.0	33.0	40.0
35	37.18021788562399	40.0	35.0	40.0	33.0	40.0
36	37.24016431194512	40.0	35.0	40.0	33.0	40.0
37	37.247431998563634	40.0	35.0	40.0	33.0	40.0
38	37.2115374106873	40.0	35.0	40.0	33.0	40.0
39	37.13547817630915	40.0	35.0	40.0	33.0	40.0
40	36.96901112847089	39.0	35.0	40.0	33.0	40.0
41	36.85568172060278	39.0	35.0	40.0	33.0	40.0
42	36.79186726128543	39.0	35.0	40.0	33.0	40.0
43	36.68618303150743	39.0	35.0	40.0	33.0	40.0
44	36.33624397658549	39.0	35.0	40.0	32.0	40.0
45	36.34680035552225	38.0	35.0	40.0	32.0	40.0
46	36.15390778948355	38.0	35.0	40.0	31.0	40.0
47	36.139408102294055	38.0	35.0	40.0	31.0	40.0
48	36.08456199279811	38.0	35.0	40.0	31.0	40.0
49	35.974437638074335	37.0	35.0	40.0	31.0	40.0
50	35.53828499546675	37.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	2.0
15	16.0
16	43.0
17	117.0
18	210.0
19	424.0
20	689.0
21	1101.0
22	1516.0
23	2238.0
24	3576.0
25	5060.0
26	6583.0
27	8867.0
28	11672.0
29	15367.0
30	20487.0
31	28321.0
32	39042.0
33	65209.0
34	129655.0
35	174075.0
36	139439.0
37	299067.0
38	483930.0
39	356707.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.28438322800373	0.624003996837324	0.054644260289569965	0.03696851486937234
8	99.41625432553927	0.4778584802873619	0.06228330484025475	0.043603889333105834
9	98.76743737377454	0.7639044550684781	0.1883554196511912	0.28030275150578393
10	62.69402894361493	29.063609264544016	2.854437727289455	5.387924064551599
11	40.383279302426985	23.216004300168727	20.40232673029935	15.998389667104936
12	37.06821276467617	14.993492872269599	24.223521789119992	23.714772573934244
13	27.241390462234683	15.885476782322916	27.595239927334287	29.277892828108115
14	25.92106246285027	17.67909098715414	30.136699865842765	26.263146684152833
15	27.786439078898507	18.51592880187441	28.43430812002556	25.263323999201525
16	34.08998905999605	19.812949351461846	24.35773478352509	21.73932680501701
17	33.81041118133085	19.775534760998273	23.19063375074857	23.223420306922314
18	31.473253864966228	20.431544681719487	23.406311300544548	24.688890152769737
19	30.063487708944596	23.061216068981132	22.385300024868716	24.48999619720556
20	30.91320595644741	24.259486633902412	21.475250053250274	23.352057356399904
21	30.81339654224503	23.820157933064127	23.536007779558364	21.83043774513248
22	29.316701404803563	21.69299070266943	25.10714178178205	23.88316611074496
23	30.577980147405682	21.136845955599863	25.06164207117359	23.22353182582086
24	32.410458688381624	20.358388284270593	22.618597560635614	24.612555466712166
25	29.92397756685837	20.51038854299444	21.310480880642437	28.255153009504756
26	26.840256984149818	21.2202063322661	23.589035015818958	28.350501667765126
27	24.9900469383044	19.75250610844767	24.368830913930832	30.888616039317103
28	26.842320083772996	22.821004361504123	21.64286295777114	28.69381259695174
29	30.952293330389235	22.740878032895846	21.15658480064324	25.150243836071677
30	29.76930085456932	20.22411953041622	23.896994454165174	26.109585160849285
31	32.03157323055751	20.745972216181617	22.70312888573662	24.519325667524246
32	35.296400504511496	20.208228087372827	21.897237565364016	22.59813384275166
33	34.6551110783989	19.64009505870912	22.717960899243792	22.986832963648183
34	29.70891337100442	21.30490493571493	27.86310832165173	21.123073371628923
35	27.086602231047085	25.569276097373844	27.047180300409607	20.296941371169467
36	26.888879223917684	28.05057159011452	22.862266353967676	22.198282832000125
37	29.903904165119343	27.804839697159277	19.788080637085166	22.503175500636218
38	30.720668578100586	26.375167417746447	20.916317333716957	21.987846670436006
39	31.351419468300197	24.560308862741426	20.823812407369616	23.264459261588765
40	29.052903450283203	22.906706635039907	20.23839394943064	27.801995965246252
41	25.5185907579828	24.829013648797993	22.100146201276	27.552249391943207
42	24.18159068326514	23.67496032715184	22.34699328321674	29.79645570636628
43	24.33035689393103	21.14833240215053	23.79601409152802	30.72529661239042
44	25.204665058564153	20.363016318560426	23.86889169173054	30.56342693114489
45	28.15606846814295	21.116103440469537	23.41528857187783	27.31253951950967
46	27.370975422349947	24.822099477087882	22.189807395710314	25.617117704851854
47	25.009785783347777	22.5289363662013	24.443325538162323	28.017952312288603
48	26.159880184095396	21.357820653076974	26.47140822719522	26.010890935632407
49	28.898003700197055	19.94693930806984	24.936071791406132	26.218985200326973
50	25.962603252560196	21.07277834838281	25.31300566850561	27.651612730551385
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	22.0
4	44.0
5	30.5
6	17.0
7	23.5
8	30.0
9	178.5
10	327.0
11	834.5
12	1342.0
13	2119.0
14	2896.0
15	2491.0
16	2086.0
17	2159.0
18	2232.0
19	2745.0
20	3258.0
21	3200.5
22	3143.0
23	3803.0
24	4463.0
25	5656.5
26	6850.0
27	7590.5
28	8331.0
29	11352.0
30	14373.0
31	15848.0
32	17323.0
33	20789.5
34	24256.0
35	25659.0
36	27062.0
37	30239.0
38	33416.0
39	37474.0
40	41532.0
41	45352.0
42	49172.0
43	52190.0
44	55208.0
45	61985.0
46	68762.0
47	70967.5
48	73173.0
49	80901.5
50	88630.0
51	93371.0
52	98112.0
53	104273.5
54	110435.0
55	126445.0
56	142455.0
57	160349.0
58	178243.0
59	183619.5
60	188996.0
61	188121.5
62	187247.0
63	155267.5
64	123288.0
65	102932.0
66	82576.0
67	67418.0
68	52260.0
69	44868.5
70	37477.0
71	31785.5
72	26094.0
73	23627.0
74	21160.0
75	15669.5
76	10179.0
77	7061.0
78	3943.0
79	2991.5
80	2040.0
81	1345.0
82	650.0
83	420.5
84	191.0
85	148.5
86	106.0
87	65.5
88	25.0
89	18.5
90	12.0
91	7.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1793418.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.343427316414037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.86374980589772	4.68978804853261
2	9.912714835877471	1.4558660181121066
3	4.154565400093229	0.9152624714061968
4	1.9955171212995133	0.5861573975569101
5	1.162641838163392	0.4268887916787441
6	0.7198069557122594	0.31715100366733406
7	0.533153066617331	0.2740619555259335
8	0.42653550566184895	0.25057859869581567
9	0.3501364896363335	0.2314081676221895
>10	9.25804662510448	20.002025159848696
>50	5.096740691996322	25.953388805439925
>100	2.3906636624585564	30.754183565947635
>500	0.08483000092594876	4.172585115003695
>1k	0.04549972776937252	5.923621105176256
>5k	0.0038559091329976713	1.8000785893798517
>10k+	0.0015423636531990686	2.2469552064061022
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	22717	1.266687409181797	No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	16960	0.945680259705211	No Hit
AACCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	7131	0.39762063278053417	No Hit
AACCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	6957	0.3879184886066717	No Hit
AACCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	6386	0.356079843070606	No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	6077	0.3388501732446089	No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	5235	0.29190071695499875	No Hit
AACCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	4455	0.24840834652044308	No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGAGGGTAAGAGTACCCCACCT	4080	0.22749855304229133	No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	3636	0.20274135756415962	No Hit
AACCTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	3130	0.1745270762309735	No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC	3127	0.17435979788314826	No Hit
AACCTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2844	0.15857987373830304	No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	2841	0.15841259539047786	No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	2679	0.1493795646079163	No Hit
AACCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2668	0.1487662106658905	No Hit
AACCTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	2397	0.13365539991234612	No Hit
AACCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	2335	0.13019831405729174	No Hit
AACCTAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC	2195	0.12239199115878172	No Hit
AACCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	2135	0.11904642420227744	No Hit
AACCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2112	0.11776395686895079	No Hit
AACCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	2101	0.11715060292692502	No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGC	1991	0.11101706350666715	No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA	1905	0.106221750869011	No Hit
AACCTAGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGC	1884	0.10505080243423451	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.033176872318667484	0.0	0.0	0.0	0.0
7	0.036578198724446835	0.0	0.0	0.0	0.0
8	0.03747034991284798	0.0	0.0	0.0	0.0
9	0.048733758666412406	0.0	0.0	0.0	0.0
10	0.07979177191262718	0.0	0.0	0.0	0.0
11	0.10538535912988495	0.0	0.0	0.0	0.0
12	0.11257832808636915	0.0	0.0	0.0	0.0
13	0.1201058537385038	0.0	0.0	0.0	0.0
14	0.13398995660799656	0.0	0.0	0.0	0.0
15	0.14714918663691343	0.0	0.0	0.0	0.0
16	0.15997385997017985	0.0	0.0	0.0	0.0
17	0.17067967423099356	0.0	0.0	0.0	0.0
18	0.18066061565123134	0.0	0.0	0.0	0.0028437319130286414
19	0.19264889724537168	0.0	0.0	0.0	0.0028437319130286414
20	0.20597540562211375	0.0	0.0	0.0	0.0028437319130286414
21	0.21840976281045468	0.0	0.0	0.0	0.0028437319130286414
22	0.22844646367996752	0.0	0.0	0.0	0.0028437319130286414
23	0.2395983535349818	0.0	0.0	0.0	0.0028437319130286414
24	0.2519769512740477	0.0	0.0	0.0	0.0028437319130286414
25	0.26234820883921095	0.0	0.0	0.0	0.0028437319130286414
26	0.27461528767972665	0.0	0.0	0.0	0.0028437319130286414
27	0.28610173423039137	0.0	0.0	0.0	0.0028437319130286414
28	0.3039447579984142	0.0	0.0	0.0	0.0028437319130286414
29	0.3138699399693769	0.0	0.0	0.0	0.0028437319130286414
30	0.3266946133026433	0.0	0.0	0.0	0.0028437319130286414
31	0.3362852385779556	0.0	0.0	0.0	0.0028437319130286414
32	0.3472140906358696	0.0	0.0	0.0	0.0028437319130286414
33	0.3579199048966833	0.0	0.0	0.0	0.0028437319130286414
34	0.36817964356329647	0.0	0.0	0.0	0.0028437319130286414
35	0.38496323779509295	0.0	0.0	0.0	0.0028437319130286414
36	0.4023601859689152	0.0	0.0	0.0	0.0028437319130286414
37	0.41652308608478333	0.0	0.0	0.0	0.0028437319130286414
38	0.429515037765875	0.0	0.0	0.0	0.0028437319130286414
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGAC	20	7.8576646E-4	44.0	42
CGCGAAT	25	4.443841E-5	44.0	40
ACGATAG	25	4.443841E-5	44.0	37
TACTCGA	25	4.443841E-5	44.0	44
CCGCGTA	25	4.443841E-5	44.0	41
ACGGTAT	30	2.5285135E-6	44.0	12
TAGGGAT	18290	0.0	42.71296	5
CTAGGGA	53120	0.0	42.587727	4
AACCTAG	184330	0.0	42.536755	1
ACCTAGG	183245	0.0	42.53049	2
CCTAGGG	181190	0.0	42.51018	3
TAGGGGA	27665	0.0	42.4334	5
CTAGGGG	113460	0.0	42.42746	4
TAGGGGG	52555	0.0	42.304634	5
TAGGGAG	17455	0.0	42.285877	5
TAGGGGT	14725	0.0	42.17725	5
TAGGGAA	9260	0.0	42.12311	5
TAGGGGC	18905	0.0	42.079876	5
TAGGGCA	3915	0.0	41.864624	5
AGGGGGG	28400	0.0	41.62183	6
>>END_MODULE