FastQCFastQC Report
Fri 17 Jun 2016
SRR1527689_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527689_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270302
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC280721.2364874805202126No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT206480.9094825269942061No Hit
AACCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC90970.4006955902782978No Hit
AACCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG88710.3907409675012399No Hit
AACCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT79610.35065819437237866No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT77750.34246545173285314No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC64640.28471983022522995No Hit
AACCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC54920.24190614288319348No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGAGGGTAAGAGTACCCCACCTGA52380.230718204009863No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA45290.19948887857210185No Hit
AACCTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT40540.17856655193890505No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT36340.16006681049481525No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC36150.15922991742948736No Hit
AACCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC34450.1517419268449748No Hit
AACCTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT34410.1515657388312216No Hit
AACCTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG31380.13821949678941392No Hit
AACCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA29640.13055531819114813No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC29460.1297624721292586No Hit
AACCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG27760.12227448154474602No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCTC27500.12112925945534998No Hit
AACCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG27040.11910309729718778No Hit
AACCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG26160.11522696099461657No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC23330.10276165902157511No Hit
AACCTAGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTC22940.10104382588748105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGT301.8622104E-646.013
TACGACT301.8622104E-646.023
CGAATAC206.3127634E-446.040
ACGTATT301.8622104E-646.039
ATACGAC253.4182165E-546.046
ACGGTAT551.8189894E-1246.012
CGAAACG351.020162E-745.99999636
TAGGGAT233750.044.7306945
CTAGGGA677000.044.4983754
AACCTAG2332600.044.4687041
ACCTAGG2317850.044.46692
CCTAGGG2291950.044.42953
CTAGGGG1443000.044.385384
TAGGGGA363300.044.360315
TAGGGGG665000.044.3225565
TAGGGAG214950.044.266575
TAGGGGT180950.044.1442385
TAGGGGC238550.043.8884965
AGGGATA28100.043.7081876
AGGGGGG361300.043.5427636
Produced by FastQC (version 0.11.5)