##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527689_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2270302
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1605099233494	33.0	31.0	34.0	31.0	34.0
2	32.75482821228189	34.0	31.0	34.0	31.0	34.0
3	32.83353051708539	34.0	31.0	34.0	31.0	34.0
4	36.45678416351657	37.0	37.0	37.0	35.0	37.0
5	36.285232977815284	37.0	37.0	37.0	35.0	37.0
6	36.22897746643398	37.0	37.0	37.0	35.0	37.0
7	36.510505210320034	37.0	37.0	37.0	35.0	37.0
8	36.6320176787053	37.0	37.0	37.0	35.0	37.0
9	38.71144279483522	39.0	39.0	39.0	39.0	39.0
10	38.12074120535506	39.0	38.0	39.0	37.0	39.0
11	37.813174194446376	39.0	38.0	39.0	35.0	39.0
12	37.1381155458613	39.0	35.0	39.0	34.0	39.0
13	36.98282739476951	39.0	35.0	39.0	34.0	39.0
14	37.71731293898345	40.0	36.0	40.0	33.0	40.0
15	37.760262291096076	40.0	36.0	40.0	33.0	40.0
16	37.79030631167131	40.0	36.0	40.0	34.0	40.0
17	37.661160057120156	40.0	36.0	40.0	33.0	40.0
18	37.6593889271119	40.0	36.0	40.0	33.0	40.0
19	37.6567602019467	40.0	36.0	40.0	33.0	40.0
20	37.640968470274	40.0	36.0	40.0	33.0	40.0
21	37.62651444609572	40.0	36.0	40.0	33.0	40.0
22	37.64261759008273	40.0	36.0	40.0	33.0	40.0
23	37.65002981982133	40.0	36.0	40.0	34.0	40.0
24	37.630327154713335	40.0	36.0	40.0	34.0	40.0
25	37.62565905328895	40.0	36.0	40.0	34.0	40.0
26	37.50018015224406	40.0	35.0	40.0	34.0	40.0
27	37.44456992946313	40.0	35.0	40.0	33.0	40.0
28	37.30783657856972	40.0	35.0	40.0	33.0	40.0
29	37.25260339813822	40.0	35.0	40.0	33.0	40.0
30	37.10673778202195	39.0	35.0	40.0	33.0	40.0
31	37.01092850202308	39.0	35.0	40.0	32.0	40.0
32	36.919178153391044	39.0	35.0	40.0	32.0	40.0
33	36.809750861339154	39.0	35.0	40.0	32.0	40.0
34	36.48007181423441	39.0	35.0	40.0	31.0	40.0
35	36.36632791584556	39.0	35.0	40.0	30.0	40.0
36	36.47338900287274	39.0	35.0	40.0	31.0	40.0
37	36.49409902294937	38.0	35.0	40.0	31.0	40.0
38	36.45648948906357	38.0	35.0	40.0	31.0	40.0
39	36.375002973172734	38.0	35.0	40.0	31.0	40.0
40	36.18099310135832	38.0	35.0	40.0	31.0	40.0
41	36.05840588608916	38.0	35.0	40.0	31.0	40.0
42	35.99573096442676	38.0	35.0	40.0	30.0	40.0
43	35.89731322088427	38.0	35.0	40.0	30.0	40.0
44	35.766636332963635	38.0	35.0	40.0	30.0	40.0
45	35.6621009011136	37.0	34.0	40.0	30.0	40.0
46	35.493155095665685	37.0	34.0	40.0	30.0	40.0
47	35.35003536974376	37.0	34.0	40.0	29.0	40.0
48	35.17720329718249	36.0	34.0	40.0	29.0	40.0
49	35.44695903892962	37.0	34.0	40.0	30.0	40.0
50	35.50606395096335	36.0	34.0	40.0	30.0	40.0
51	35.52340041104664	36.0	35.0	40.0	30.0	40.0
52	35.05979644998771	36.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	7.0
15	17.0
16	55.0
17	149.0
18	312.0
19	706.0
20	1155.0
21	2073.0
22	3107.0
23	4513.0
24	6542.0
25	9238.0
26	12588.0
27	16578.0
28	21956.0
29	27549.0
30	35733.0
31	46661.0
32	61094.0
33	87193.0
34	196367.0
35	210520.0
36	194200.0
37	314287.0
38	668740.0
39	348961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.33947113643912	0.6115485957374833	0.02175921969852469	0.027221048124875014
8	99.42020929374154	0.4937228615400066	0.051490947019383324	0.03457689769907263
9	98.7712207450815	0.7769891406517724	0.17473446263977216	0.27705565162696416
10	62.77001914282769	29.043580986141933	2.835173470313641	5.351226400716732
11	40.3966961223661	23.260649904726332	20.330334906985943	16.012319065921627
12	37.05502615951534	14.965850358234277	24.188235750133682	23.790887732116698
13	27.255580975570652	15.912949026164803	27.54897806547323	29.28249193279132
14	25.878010943037534	17.648004538603235	30.209901590184916	26.264082928174314
15	27.832949096640007	18.472740630982134	28.43383831754542	25.260471954832443
16	34.10202695500422	19.800978019664345	24.376624783839333	21.720370241492102
17	33.885315698087744	19.694472365350514	23.14035753833631	23.27985439822543
18	31.529417672186344	20.34134665784552	23.384554125398296	24.74468154456984
19	30.155635681948922	22.992844123821413	22.40186547868962	24.44965471554005
20	30.797136240024454	24.330948041273803	21.439834876593512	23.43208084210823
21	30.800615953296077	23.772123708651975	23.53131874085474	21.8959415971972
22	29.385121450802583	21.653550937276187	25.069660335937687	23.891667275983547
23	30.607734125239727	21.068078167574182	25.04279166384032	23.281396043345776
24	32.41154701004536	20.229423222108778	22.65795475667995	24.701075011165916
25	29.999489054760115	20.44349165881896	21.25047680881222	28.306542477608705
26	26.82770838417092	21.17621356101523	23.557835036924605	28.43824301788925
27	25.033673934128586	19.63906123502512	24.27998565829568	31.047279172550613
28	26.803834908307355	22.81233950373122	21.681212455435446	28.702613132525983
29	30.996669165599993	22.666323687333225	21.13947836014768	25.1975287869191
30	29.821010596828085	20.203964054121435	23.93122148507115	26.04380386397933
31	32.14951138659086	20.66434333405864	22.67147718673551	24.51466809261499
32	35.410002722104814	20.066845732417978	21.93584818231231	22.5873033631649
33	34.80475284785901	19.589464309153584	22.638441934156777	22.96734090883063
34	29.53752408269913	21.313287835715247	27.896773204622114	21.252414876963506
35	27.15220265850094	25.581486515890838	26.99742148841872	20.268889337189503
36	26.907741789418328	28.08172657205958	22.81890250724353	22.19162913127857
37	29.948526671781995	27.756395404664225	19.743672868191105	22.55140505536268
38	30.754895163727113	26.349886490872137	20.842381321956285	22.052837023444457
39	31.38393041982961	24.571929197084792	20.633025914614002	23.411114468471595
40	29.103484910818032	22.853787733966673	20.220129304383295	27.822598050832003
41	25.625621613336026	24.834493384580554	22.05715362978141	27.48273137230201
42	24.17114551279962	23.735168272767236	22.222990597726646	29.8706956167065
43	24.31980414940391	21.165950609214104	23.748866890836553	30.765378350545436
44	25.113707339375996	20.235898131614206	23.861803407652374	30.788591121357424
45	28.233204216883923	21.02332641208086	23.423315488424006	27.320153882611216
46	27.242410921542596	24.687816863130983	22.253427077102515	25.8163451382239
47	25.108465745966836	22.401425008655238	24.484539942263186	28.00556930311474
48	26.33680453085096	21.16921008746854	26.430404413157373	26.06358096852313
49	28.931084939360492	19.811593347492977	24.955270268008398	26.302051445138137
50	25.96566447987977	21.001875521406404	25.30918794063521	27.723272058078617
51	24.483086391149726	20.982362698883232	26.69433405775972	27.840216852207327
52	24.535502325241314	21.43124571092304	26.06534284866066	27.967909115174987
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	24.5
4	49.0
5	34.5
6	20.0
7	22.5
8	25.0
9	221.0
10	417.0
11	981.0
12	1545.0
13	2354.5
14	2652.5
15	2141.0
16	2272.0
17	2403.0
18	3120.0
19	3837.0
20	3690.0
21	3543.0
22	3889.0
23	4235.0
24	5129.5
25	6024.0
26	7921.5
27	9819.0
28	11202.5
29	12586.0
30	16022.0
31	19458.0
32	19996.0
33	20534.0
34	27340.0
35	34146.0
36	34108.5
37	34071.0
38	39390.0
39	48246.5
40	51784.0
41	57633.0
42	63482.0
43	65891.0
44	68300.0
45	75076.0
46	81852.0
47	86821.0
48	91790.0
49	96364.0
50	100938.0
51	111759.0
52	122580.0
53	130387.0
54	138194.0
55	155279.0
56	172364.0
57	197293.5
58	222223.0
59	225473.5
60	228724.0
61	225626.5
62	222529.0
63	189285.0
64	139071.5
65	122102.0
66	98535.0
67	74968.0
68	63056.5
69	51145.0
70	45880.0
71	40615.0
72	35965.5
73	31316.0
74	22987.5
75	14659.0
76	10919.5
77	7180.0
78	4953.5
79	2727.0
80	2182.0
81	1637.0
82	968.5
83	300.0
84	179.5
85	59.0
86	42.5
87	26.0
88	24.5
89	19.0
90	15.0
91	9.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2270302.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.273715395934258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.21699611956437	4.816435841473943
2	9.994570524786301	1.453953230037777
3	4.343213471673892	0.9477389609023039
4	2.3316097419623296	0.6783786270968679
5	1.2533341852892521	0.45581980798945754
6	0.758813840975516	0.3311637550650972
7	0.5005554636777878	0.25486285878704956
8	0.3768022665067125	0.2192601957690238
9	0.29596647427871825	0.19374983105773438
>10	6.054884424449521	12.73548858626546
>50	4.8623576401704565	25.28710050270986
>100	2.8480301754902007	36.5775865600506
>500	0.10655895214419119	5.094209953189836
>1k	0.05085768170518216	6.567213294542351
>5k	0.004238140142098513	2.241477041023689
>10k+	0.001210897183456718	2.145560954038943
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	28072	1.2364874805202126	No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	20648	0.9094825269942061	No Hit
AACCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	9097	0.4006955902782978	No Hit
AACCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	8871	0.3907409675012399	No Hit
AACCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	7961	0.35065819437237866	No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	7775	0.34246545173285314	No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	6464	0.28471983022522995	No Hit
AACCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	5492	0.24190614288319348	No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGAGGGTAAGAGTACCCCACCTGA	5238	0.230718204009863	No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4529	0.19948887857210185	No Hit
AACCTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	4054	0.17856655193890505	No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	3634	0.16006681049481525	No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	3615	0.15922991742948736	No Hit
AACCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3445	0.1517419268449748	No Hit
AACCTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	3441	0.1515657388312216	No Hit
AACCTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	3138	0.13821949678941392	No Hit
AACCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	2964	0.13055531819114813	No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	2946	0.1297624721292586	No Hit
AACCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2776	0.12227448154474602	No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCTC	2750	0.12112925945534998	No Hit
AACCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2704	0.11910309729718778	No Hit
AACCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2616	0.11522696099461657	No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	2333	0.10276165902157511	No Hit
AACCTAGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTC	2294	0.10104382588748105	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.031713842475582545	0.0	0.0	0.0	0.0
7	0.03484117971970249	0.0	0.0	0.0	0.0
8	0.035898307802221904	0.0	0.0	0.0	0.0
9	0.047967186744318595	0.0	0.0	0.0	0.0
10	0.07479181183824883	0.0	0.0	0.0	0.0
11	0.09716768958490984	0.0	0.0	0.0	0.0
12	0.10474377417629901	0.0	0.0	0.0	0.0
13	0.11157105970923692	0.0	0.0	0.0	0.0
14	0.12421254969603163	0.0	0.0	0.0	0.0
15	0.13650166365531988	0.0	0.0	0.0	0.0
16	0.14790983754584192	0.0	0.0	0.0	0.0
17	0.15856921237791272	0.0	0.0	0.0	0.0
18	0.1681714591274641	0.0	0.0	0.0	0.0036559012853796544
19	0.18054866709362896	0.0	0.0	0.0	0.0036559012853796544
20	0.1923973110185341	0.0	0.0	0.0	0.0036559012853796544
21	0.20266026281966013	0.0	0.0	0.0	0.0036559012853796544
22	0.21138156950044532	0.0	0.0	0.0	0.0036559012853796544
23	0.2223933203600226	0.0	0.0	0.0	0.0036559012853796544
24	0.23336102421616156	0.0	0.0	0.0	0.0036559012853796544
25	0.24256684793476815	0.0	0.0	0.0	0.0036559012853796544
26	0.2543273978527967	0.0	0.0	0.0	0.0036559012853796544
27	0.2648986786779909	0.0	0.0	0.0	0.0036559012853796544
28	0.2802710828779607	0.0	0.0	0.0	0.0036559012853796544
29	0.2903137996618952	0.0	0.0	0.0	0.0036559012853796544
30	0.30176602055585555	0.0	0.0	0.0	0.0036559012853796544
31	0.3112801732985303	0.0	0.0	0.0	0.0036559012853796544
32	0.32273239419249067	0.0	0.0	0.0	0.0036559012853796544
33	0.33334772202112317	0.0	0.0	0.0	0.0036559012853796544
34	0.34378686183600243	0.0	0.0	0.0	0.0036559012853796544
35	0.3602163941184917	0.0	0.0	0.0	0.0036559012853796544
36	0.3759411743459681	0.0	0.0	0.0	0.0036559012853796544
37	0.3895957454118439	0.0	0.0	0.0	0.0036559012853796544
38	0.40073963728173606	0.0	0.0	0.0	0.0036559012853796544
39	0.41135496511036856	0.0	0.0	0.0	0.0036559012853796544
40	0.4232036090352737	0.0	0.0	0.0	0.0036559012853796544
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCGT	30	1.8622104E-6	46.0	13
TACGACT	30	1.8622104E-6	46.0	23
CGAATAC	20	6.3127634E-4	46.0	40
ACGTATT	30	1.8622104E-6	46.0	39
ATACGAC	25	3.4182165E-5	46.0	46
ACGGTAT	55	1.8189894E-12	46.0	12
CGAAACG	35	1.020162E-7	45.999996	36
TAGGGAT	23375	0.0	44.730694	5
CTAGGGA	67700	0.0	44.498375	4
AACCTAG	233260	0.0	44.468704	1
ACCTAGG	231785	0.0	44.4669	2
CCTAGGG	229195	0.0	44.4295	3
CTAGGGG	144300	0.0	44.38538	4
TAGGGGA	36330	0.0	44.36031	5
TAGGGGG	66500	0.0	44.322556	5
TAGGGAG	21495	0.0	44.26657	5
TAGGGGT	18095	0.0	44.144238	5
TAGGGGC	23855	0.0	43.888496	5
AGGGATA	2810	0.0	43.708187	6
AGGGGGG	36130	0.0	43.542763	6
>>END_MODULE