FastQCFastQC Report
Fri 17 Jun 2016
SRR1527688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284717
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC265401.1616318344897858No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT193870.8485514836191966No Hit
AACCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC86730.3796093783168769No Hit
AACCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG84500.3698488696849544No Hit
AACCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT77100.3374597379018933No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT74810.32743661468794605No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC61250.2680857191503368No Hit
AACCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC53400.23372697800208955No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGAGGGTAAGAGTACCCCACCTGA50800.22234701278101401No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA43880.1920587976541515No Hit
AACCTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT40110.17555784808359198No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT35370.1548112961036312No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC35050.15341068499949884No Hit
AACCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC33610.14710793503090316No Hit
AACCTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT31790.13914195937615031No Hit
AACCTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG29950.13108844552738919No Hit
AACCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA28010.12259724070858666No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC27350.11970848030631366No Hit
AACCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG26830.11743248726209855No Hit
AACCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG25290.11069204632346151No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCTC25190.11025435535342014No Hit
AACCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG24380.10670905849608507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAC301.8622159E-646.015
CGCGAAT253.418223E-546.040
TAACGCG301.8622159E-646.034
CGACTAA600.046.022
ACGGTAT501.6370905E-1146.012
TAGGGAT236500.044.667655
CTAGGGA670500.044.548994
AACCTAG2346100.044.4735951
ACCTAGG2334550.044.454222
TAGGGAG216100.044.4460875
CCTAGGG2308350.044.4346853
CTAGGGG1454650.044.354044
TAGGGGA361950.044.3287735
TAGGGGG672550.044.2729955
TAGGGGT189150.044.0301365
TAGGGGC236400.043.869295
AGGGATT81200.043.7623146
TAGGGAC111800.043.6753125
CGATTAG950.043.5789542
AGGGGGG366350.043.526416
Produced by FastQC (version 0.11.5)