##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527688_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2284717
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13059691856803	33.0	31.0	34.0	31.0	34.0
2	32.739756389959894	34.0	31.0	34.0	31.0	34.0
3	32.82433491762875	34.0	31.0	34.0	31.0	34.0
4	36.44691399416208	37.0	37.0	37.0	35.0	37.0
5	36.29703459990887	37.0	37.0	37.0	35.0	37.0
6	36.16390476369721	37.0	37.0	37.0	35.0	37.0
7	36.47106096728829	37.0	37.0	37.0	35.0	37.0
8	36.60469589887938	37.0	37.0	37.0	35.0	37.0
9	38.666746034629234	39.0	39.0	39.0	38.0	39.0
10	38.02792512157961	39.0	38.0	39.0	37.0	39.0
11	37.77903040070171	39.0	38.0	39.0	35.0	39.0
12	37.08884338848094	39.0	35.0	39.0	34.0	39.0
13	36.79083799000051	39.0	35.0	39.0	33.0	39.0
14	37.65781495038554	40.0	36.0	40.0	33.0	40.0
15	37.71597138726591	40.0	36.0	40.0	33.0	40.0
16	37.78348915861352	40.0	36.0	40.0	34.0	40.0
17	37.681766713339115	40.0	36.0	40.0	33.0	40.0
18	37.6958200950052	40.0	36.0	40.0	33.0	40.0
19	37.64800717112885	40.0	36.0	40.0	33.0	40.0
20	37.642489201069544	40.0	36.0	40.0	33.0	40.0
21	37.68491414910468	40.0	36.0	40.0	33.0	40.0
22	37.5875961880618	40.0	36.0	40.0	33.0	40.0
23	37.62358445269152	40.0	36.0	40.0	34.0	40.0
24	37.65093926293716	40.0	36.0	40.0	34.0	40.0
25	37.61439250462968	40.0	36.0	40.0	34.0	40.0
26	37.44371316009816	40.0	35.0	40.0	34.0	40.0
27	37.39001591882058	40.0	35.0	40.0	33.0	40.0
28	37.2742895509597	40.0	35.0	40.0	33.0	40.0
29	37.215821478108666	40.0	35.0	40.0	33.0	40.0
30	37.0640713926495	39.0	35.0	40.0	32.0	40.0
31	36.98215096224171	39.0	35.0	40.0	32.0	40.0
32	36.89451166161936	39.0	35.0	40.0	32.0	40.0
33	36.59167503021162	39.0	35.0	40.0	31.0	40.0
34	36.38665313909775	39.0	35.0	40.0	30.0	40.0
35	36.35658595791076	39.0	35.0	40.0	30.0	40.0
36	36.315355468532864	39.0	35.0	40.0	31.0	40.0
37	36.41269137490551	38.0	35.0	40.0	31.0	40.0
38	36.102066908067826	38.0	35.0	40.0	30.0	40.0
39	36.19811031300594	38.0	35.0	40.0	31.0	40.0
40	36.0830584269299	38.0	35.0	40.0	30.0	40.0
41	35.76765043548063	38.0	35.0	40.0	30.0	40.0
42	35.84026292971952	38.0	35.0	40.0	30.0	40.0
43	35.85255416754023	38.0	35.0	40.0	30.0	40.0
44	35.72003841175953	38.0	35.0	40.0	30.0	40.0
45	35.61368694678597	37.0	34.0	40.0	30.0	40.0
46	35.26963908440301	37.0	34.0	40.0	29.0	40.0
47	35.04014807960898	37.0	34.0	40.0	28.0	40.0
48	35.03416878326725	36.0	34.0	40.0	28.0	40.0
49	34.97431323004118	36.0	34.0	40.0	28.0	40.0
50	34.83092785671048	36.0	34.0	40.0	28.0	40.0
51	35.15860257528613	36.0	34.0	40.0	29.0	40.0
52	34.90291182671639	35.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	5.0
15	17.0
16	61.0
17	136.0
18	371.0
19	723.0
20	1216.0
21	2145.0
22	3147.0
23	4666.0
24	6862.0
25	9607.0
26	13055.0
27	17763.0
28	22632.0
29	29279.0
30	37062.0
31	48447.0
32	64397.0
33	95672.0
34	203452.0
35	209054.0
36	207760.0
37	337390.0
38	665453.0
39	304341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.33151458145582	0.614824505617107	0.024423156128308234	0.02923775679876326
8	99.42469898897764	0.48820050798413983	0.051034767106823295	0.03606573593140857
9	98.75336857912818	0.7768139336294166	0.19162110668411012	0.2781963805582923
10	62.72273546351692	29.043947237228945	2.8782558189920238	5.355061480262107
11	40.338212566370366	23.277893936097993	20.349566270133238	16.034327227398403
12	36.99985599967086	15.000501156160698	24.2535071083202	23.74613573584825
13	27.476794718995833	15.91566920542019	27.496403274453684	29.11113280113029
14	25.82709368381292	17.660086566520057	30.183563215925645	26.32925653374138
15	27.756129096076233	18.50483013869989	28.46352524185709	25.27551552336679
16	34.16935226551034	19.772076804260657	24.366124994911843	21.692445935317153
17	33.79989731769843	19.785032456973884	23.131267461134136	23.283802764193553
18	31.45361110369468	20.41128069690907	23.396727034464224	24.738381164932026
19	30.099395242386695	23.014885432200135	22.402336919627245	24.483382405785925
20	30.80994276315185	24.30143426953973	21.472287377386344	23.416335589922078
21	30.748228336376016	23.83047878577522	23.538801523339654	21.882491354509114
22	29.45577067094087	21.66530909517459	25.068312618149207	23.81060761573534
23	30.561903290429406	21.083749103280624	25.045246304028023	23.309101302261944
24	32.34886421381729	20.291659754796765	22.676944234231197	24.68253179715475
25	29.941738954977794	20.514225613062802	21.240573777846446	28.30346165411296
26	26.963645825719336	21.191902541977846	23.582658158537797	28.261793473765024
27	25.074265215341768	19.622867952573557	24.256220792334453	31.046646039750218
28	26.824241251761162	22.797265481895572	21.689513405817877	28.688979860525393
29	31.005328012178314	22.693445183801757	21.07228159986554	25.228945204154385
30	29.78876596094834	20.31879659493933	23.851531721434206	26.040905722678126
31	32.11286124277098	20.703789572187713	22.675543623127066	24.507805561914235
32	35.34980481171191	20.12770071741927	21.960575423564492	22.561919047304325
33	34.66845127864852	19.692767200489165	22.641578803851857	22.997202717010463
34	29.519673552566907	21.29953075151102	27.912122157798976	21.268673538123103
35	27.152421941098176	25.546008542852356	27.030218622262623	20.271350893786845
36	27.110009686101165	28.038571079043926	22.815954886316337	22.03546434853857
37	29.951368156318704	27.73336916563408	19.80267140306655	22.512591274980664
38	30.77431471819048	26.32824984451028	20.789051773151773	22.108383664147464
39	31.359463776038783	24.602040427764138	20.637960850293492	23.400534945903583
40	29.125138912171618	22.917105269492897	20.180661324794276	27.777094493541217
41	25.907147362233484	24.77685420119866	22.034632735695492	27.281365700872364
42	24.15734640220211	23.697902190949687	22.255141446402334	29.889609960445867
43	24.361266625144385	21.20420165823601	23.76193637986674	30.672595336752867
44	25.05522565814497	20.265792218467322	23.880419325456938	30.798562797930774
45	28.303417885015957	20.989251622848695	23.38622245118323	27.32110804095212
46	27.371354964312864	24.70038958873243	22.221307934418135	25.706947512536566
47	25.31341956137237	22.52292078187364	24.37247151397744	27.791188142776548
48	26.310654667514623	21.205646038437145	26.43097591517899	26.052723378869242
49	28.985778107310445	19.849679413248992	24.929258196967062	26.235284282473497
50	25.932008209331837	21.04969674581141	25.27328329941958	27.745011745437182
51	24.441495379952965	20.987894780841565	26.735346215745757	27.83526362345971
52	24.628695807839655	21.31213625144821	26.119033560830513	27.94013437988162
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	28.0
4	56.0
5	38.0
6	20.0
7	25.5
8	31.0
9	192.5
10	354.0
11	955.0
12	1556.0
13	2314.5
14	2661.0
15	2249.0
16	2387.5
17	2526.0
18	3135.5
19	3745.0
20	3778.0
21	3811.0
22	4014.5
23	4218.0
24	5153.0
25	6088.0
26	8020.5
27	9953.0
28	11355.5
29	12758.0
30	16104.5
31	19451.0
32	20104.0
33	20757.0
34	27426.0
35	34095.0
36	34251.5
37	34408.0
38	39787.5
39	48617.5
40	52068.0
41	57864.5
42	63661.0
43	66462.0
44	69263.0
45	75957.0
46	82651.0
47	87420.0
48	92189.0
49	96822.5
50	101456.0
51	112268.5
52	123081.0
53	131531.5
54	139982.0
55	157041.5
56	174101.0
57	198551.5
58	223002.0
59	226791.5
60	230581.0
61	226989.5
62	223398.0
63	190332.5
64	139758.5
65	122250.0
66	99094.0
67	75938.0
68	63674.0
69	51410.0
70	45944.5
71	40479.0
72	35858.5
73	31238.0
74	22951.5
75	14665.0
76	10844.5
77	7024.0
78	4863.0
79	2702.0
80	2137.0
81	1572.0
82	927.5
83	283.0
84	170.0
85	57.0
86	42.0
87	27.0
88	28.5
89	26.5
90	23.0
91	13.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2284717.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.602689090229648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.89722593895314	5.009961207224771
2	10.12884615633122	1.5401293633870772
3	4.5080315356595175	1.0281948652370942
4	2.289145033960516	0.6961463190257996
5	1.2408089507072204	0.47167423363005406
6	0.8038981356308856	0.36670725512501323
7	0.5339171293882824	0.28414441542809177
8	0.37982561962262906	0.23101568755957425
9	0.32341487424256515	0.22129404624197493
>10	6.378882802918293	14.000562872719149
>50	4.766570000487384	25.74891119091599
>100	2.6004408089219284	34.942969085094276
>500	0.09530727158656105	4.739379085275162
>1k	0.0482568463729423	6.372003092820794
>5k	0.004222474057632451	2.2406847299065173
>10k+	0.0012064211593235577	2.106222550408658
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	26540	1.1616318344897858	No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	19387	0.8485514836191966	No Hit
AACCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	8673	0.3796093783168769	No Hit
AACCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	8450	0.3698488696849544	No Hit
AACCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	7710	0.3374597379018933	No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	7481	0.32743661468794605	No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	6125	0.2680857191503368	No Hit
AACCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	5340	0.23372697800208955	No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGAGGGTAAGAGTACCCCACCTGA	5080	0.22234701278101401	No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4388	0.1920587976541515	No Hit
AACCTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	4011	0.17555784808359198	No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	3537	0.1548112961036312	No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	3505	0.15341068499949884	No Hit
AACCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3361	0.14710793503090316	No Hit
AACCTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	3179	0.13914195937615031	No Hit
AACCTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	2995	0.13108844552738919	No Hit
AACCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	2801	0.12259724070858666	No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	2735	0.11970848030631366	No Hit
AACCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2683	0.11743248726209855	No Hit
AACCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2529	0.11069204632346151	No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCTC	2519	0.11025435535342014	No Hit
AACCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2438	0.10670905849608507	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.03090098248492045	0.0	0.0	0.0	0.0
7	0.034314972051243106	0.0	0.0	0.0	0.0
8	0.03554050676735893	0.0	0.0	0.0	0.0
9	0.04600132095134758	0.0	0.0	0.0	0.0
10	0.07134362811674269	0.0	0.0	0.0	0.0
11	0.09134610544763312	0.0	0.0	0.0	0.0
12	0.09773639361023706	0.0	0.0	0.0	0.0
13	0.10487075642191133	0.0	0.0	0.0	0.0
14	0.11668841261302822	0.0	0.0	0.0	0.0
15	0.12618630666292588	0.0	0.0	0.0	0.0
16	0.1368659663319352	0.0	0.0	0.0	0.0
17	0.14671401315786595	0.0	0.0	0.0	0.0
18	0.15638698359578013	0.0	0.0	0.0	0.0036766041483474758
19	0.1661474922277026	0.0	0.0	0.0	0.0036766041483474758
20	0.17827153209784843	0.0	0.0	0.0	0.0036766041483474758
21	0.18811957892377917	0.0	0.0	0.0	0.0036766041483474758
22	0.197354858391652	0.0	0.0	0.0	0.0036766041483474758
23	0.2088223618067358	0.0	0.0	0.0	0.0036766041483474758
24	0.220421172512832	0.0	0.0	0.0	0.0036766041483474758
25	0.23101329398783307	0.0	0.0	0.0	0.0036766041483474758
26	0.24261210469392927	0.0	0.0	0.0	0.0036766041483474758
27	0.25132215499775246	0.0	0.0	0.0	0.0036766041483474758
28	0.26637872436717547	0.0	0.0	0.0	0.0036766041483474758
29	0.2761392329990979	0.0	0.0	0.0	0.0036766041483474758
30	0.28769427460818997	0.0	0.0	0.0	0.0036766041483474758
31	0.2963167867180049	0.0	0.0	0.0	0.0036766041483474758
32	0.30774052103608457	0.0	0.0	0.0	0.0036766041483474758
33	0.31815756612306906	0.0	0.0	0.0	0.0036766041483474758
34	0.32721776920292533	0.0	0.0	0.0	0.0036766041483474758
35	0.34406887154951793	0.0	0.0	0.0	0.0036766041483474758
36	0.3589503645309244	0.0	0.0	0.0	0.0036766041483474758
37	0.3724750155052026	0.0	0.0	0.0	0.0036766041483474758
38	0.3839425189202864	0.0	0.0	0.0	0.0036766041483474758
39	0.39330910567917166	0.0	0.0	0.0	0.0036766041483474758
40	0.4033322288931189	0.0	0.0	0.0	0.0036766041483474758
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGAC	30	1.8622159E-6	46.0	15
CGCGAAT	25	3.418223E-5	46.0	40
TAACGCG	30	1.8622159E-6	46.0	34
CGACTAA	60	0.0	46.0	22
ACGGTAT	50	1.6370905E-11	46.0	12
TAGGGAT	23650	0.0	44.66765	5
CTAGGGA	67050	0.0	44.54899	4
AACCTAG	234610	0.0	44.473595	1
ACCTAGG	233455	0.0	44.45422	2
TAGGGAG	21610	0.0	44.446087	5
CCTAGGG	230835	0.0	44.434685	3
CTAGGGG	145465	0.0	44.35404	4
TAGGGGA	36195	0.0	44.328773	5
TAGGGGG	67255	0.0	44.272995	5
TAGGGGT	18915	0.0	44.030136	5
TAGGGGC	23640	0.0	43.86929	5
AGGGATT	8120	0.0	43.762314	6
TAGGGAC	11180	0.0	43.675312	5
CGATTAG	95	0.0	43.57895	42
AGGGGGG	36635	0.0	43.52641	6
>>END_MODULE