FastQCFastQC Report
Fri 17 Jun 2016
SRR1527687_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527687_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505141
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC49850.9868531756479874No Hit
TGTCACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT37340.7391995502245907No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG31980.6330905628329515No Hit
TGTCACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT24360.4822415919515541No Hit
TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC23250.46026752926410647No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG15800.31278395537087667No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC14910.29516511231517534No Hit
TGTCACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC12810.25359256128486896No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT12470.24686176730853363No Hit
TGTCACGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCT12130.24013097333219832No Hit
TGTCACGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC11760.23280628576971577No Hit
TGTCACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA11510.22785717255182214No Hit
TGTCACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT10700.21182204572584687No Hit
TGTCACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC10650.2108322230822681No Hit
TGTCACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG10050.19895435135932343No Hit
TGTCACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG9830.19459913172757706No Hit
TGTCACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC8790.1740108207411396No Hit
TGTCACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC8720.1726250690401294No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGTAAGAGCACCCGACTGT8660.17143728186783494No Hit
TGTCACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG8550.16925967205196174No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT8460.16747799129352003No Hit
TGTCACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA8350.16530038147764683No Hit
TGTCACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG7710.1526306516398392No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTG7510.14867136106552428No Hit
TGTCACGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTG7300.14451410596249364No Hit
TGTCACGGGGGTTTTTCAGGACGCTTTGCAAGGGTAGCTGGGGTGAATTT7050.13956499274460002No Hit
TGTCACGGGGCGTTTCCGTTGCACGGAGTTGTGAAGAGTACTGCCTTGTC6730.13323012782569618No Hit
TGTCACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA6670.13204234065340173No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT6500.12867694366523408No Hit
TGTCACGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAA6450.12768712102165533No Hit
TGTCACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG6200.12273800780376173No Hit
TGTCACGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC6030.11937261081559405No Hit
TGTCACGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG5910.11699703647100512No Hit
TGTCACGGGAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCC5820.11521535571256342No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCT5700.11283978136797448No Hit
TGTCACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC5690.11264181683925874No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGC5620.11125606513824854No Hit
TGTCACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC5540.10967234890852257No Hit
TGTCACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAC5290.10472323569062897No Hit
TGTCACGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT5280.10452527116191321No Hit
TGTCACGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGC5200.10294155493218726No Hit
TGTCACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC5060.10017005153016682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCAT302.5248883E-644.00000434
CGAACTA302.5248883E-644.00000440
ATTAACG302.5248883E-644.00000429
AGTGCGT302.5248883E-644.00000415
ATATACG302.5248883E-644.00000434
ACGTGGG302.5248883E-644.00000410
CCATAAC302.5248883E-644.00000434
TATTAAC302.5248883E-644.00000428
CGACTGA302.5248883E-644.00000444
TATACGG302.5248883E-644.00000435
ACGCGAA207.852286E-444.025
CGGGTAT551.8189894E-1244.06
AAGTACG207.852286E-444.038
AGCGTAC254.439286E-544.036
AACCGAC207.852286E-444.026
TCGCATA207.852286E-444.025
CGTATTA207.852286E-444.030
CATATAG408.296411E-944.013
CGTATCG254.439286E-544.028
GTATTAC207.852286E-444.031
Produced by FastQC (version 0.11.5)