##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527687_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	505141
Sequences flagged as poor quality	0
Sequence length	50
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53205936560287	33.0	31.0	34.0	31.0	34.0
2	32.889308133768594	34.0	31.0	34.0	31.0	34.0
3	31.822803929991824	31.0	31.0	34.0	31.0	34.0
4	36.27592691941458	37.0	37.0	37.0	35.0	37.0
5	36.30030229183535	37.0	37.0	37.0	35.0	37.0
6	36.44518659146654	37.0	37.0	37.0	35.0	37.0
7	36.50090964700945	37.0	37.0	37.0	35.0	37.0
8	36.66814414193265	37.0	37.0	37.0	35.0	37.0
9	38.758558501487705	39.0	39.0	39.0	39.0	39.0
10	38.269889001288746	39.0	39.0	39.0	37.0	39.0
11	37.98555254869432	39.0	38.0	39.0	35.0	39.0
12	37.20169616008204	39.0	35.0	39.0	35.0	39.0
13	37.03051029316567	39.0	35.0	39.0	34.0	39.0
14	37.72902615309389	40.0	37.0	40.0	33.0	40.0
15	37.76397481099337	40.0	37.0	40.0	33.0	40.0
16	37.796201456623	40.0	36.0	40.0	33.0	40.0
17	37.63558887518534	40.0	36.0	40.0	33.0	40.0
18	37.50557567095128	40.0	36.0	40.0	33.0	40.0
19	37.37310176762528	40.0	36.0	40.0	32.0	40.0
20	37.28429883933397	40.0	36.0	40.0	32.0	40.0
21	37.22973189663876	39.0	35.0	40.0	32.0	40.0
22	37.348958013703104	40.0	35.0	40.0	33.0	40.0
23	37.34173824734084	39.0	35.0	40.0	33.0	40.0
24	37.28823833345541	39.0	35.0	40.0	33.0	40.0
25	37.31244345638149	39.0	35.0	40.0	33.0	40.0
26	37.21562296467719	39.0	35.0	40.0	33.0	40.0
27	37.03794584086423	39.0	35.0	40.0	33.0	40.0
28	36.85494545087411	39.0	35.0	40.0	32.0	40.0
29	37.03032024721811	39.0	35.0	40.0	33.0	40.0
30	37.200840953317986	40.0	35.0	40.0	33.0	40.0
31	37.26392433003854	40.0	35.0	40.0	33.0	40.0
32	37.23381590486617	40.0	35.0	40.0	33.0	40.0
33	37.088420856750886	39.0	35.0	40.0	33.0	40.0
34	36.755660300787305	39.0	35.0	40.0	32.0	40.0
35	36.71259509721048	39.0	35.0	40.0	32.0	40.0
36	36.705169447738356	39.0	35.0	40.0	32.0	40.0
37	36.654211398401635	39.0	35.0	40.0	32.0	40.0
38	36.63885133061858	39.0	35.0	40.0	32.0	40.0
39	36.505627141728745	38.0	35.0	40.0	32.0	40.0
40	36.304425892968496	38.0	35.0	40.0	32.0	40.0
41	36.125925236716085	38.0	35.0	40.0	31.0	40.0
42	36.09995822948444	38.0	35.0	40.0	31.0	40.0
43	35.98906641907903	37.0	35.0	40.0	31.0	40.0
44	35.72676539817596	37.0	35.0	40.0	31.0	40.0
45	35.643952876523585	37.0	35.0	40.0	31.0	40.0
46	35.505070861403055	36.0	35.0	40.0	30.0	40.0
47	35.38883796801289	36.0	34.0	40.0	30.0	40.0
48	35.3038240808012	36.0	34.0	40.0	30.0	40.0
49	35.20461019794473	35.0	34.0	40.0	30.0	40.0
50	34.68646179977472	35.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	11.0
16	26.0
17	60.0
18	84.0
19	120.0
20	234.0
21	375.0
22	570.0
23	872.0
24	1177.0
25	1642.0
26	2391.0
27	3095.0
28	4246.0
29	5642.0
30	7723.0
31	10176.0
32	13526.0
33	20051.0
34	47426.0
35	42821.0
36	49075.0
37	78769.0
38	154130.0
39	60896.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.39482243571597	0.5190629942926827	0.058597500499860435	0.027517069491488518
8	99.65178039398901	0.20390346457721703	0.06117103937316511	0.08314510206061278
9	98.37134582225558	0.8385777436398946	0.2684399009385498	0.5216365331659872
10	60.928532825488325	29.22035629655878	3.310758778242114	6.540352099710773
11	40.94421161616262	23.30379042683132	19.520490318544724	16.23150763846134
12	35.56987059058758	14.995219156631515	23.73238363150091	25.702526621279997
13	27.503805868064564	15.538235858898803	28.242807453760438	28.715150819276204
14	26.149134598062716	17.48541496334687	30.997879799897454	25.36757063869296
15	29.582631384108595	17.92351046539481	28.838284756137394	23.6555733943592
16	34.102953432803915	18.912541250858673	23.720505759777964	23.263999556559455
17	32.90526803407366	20.666704939808884	22.93577436794875	23.49225265816871
18	33.04305134605981	20.59246824154048	21.998214359950985	24.366266052448722
19	30.30539987844978	22.68732888441049	22.899942788251202	24.10732844888853
20	32.96287571192993	22.408396863450005	22.456898172985365	22.17182925163469
21	31.827747104273858	22.588544584581335	23.755347516831936	21.828360794312875
22	30.073979344381073	20.709861207068915	25.272349700380687	23.943809748169322
23	30.394087987314432	22.30743495380498	24.351616677323758	22.94686038155683
24	31.07666968232632	20.034010306033366	23.594402354986034	25.29491765665428
25	29.99776300082551	21.52290152650448	22.25517231822402	26.224163154445986
26	27.59447362221637	20.842497441308467	23.867395440085044	27.695633496390116
27	24.89898859922279	20.157738136480706	23.99468663204927	30.94858663224723
28	27.001173929655288	23.123444741171276	21.808366376912584	28.067014952260855
29	28.739302491779522	22.70554162105234	22.004351260341174	26.550804626826963
30	29.303303434090676	20.414102201167594	24.584422963093473	25.698171401648253
31	32.66276148639687	20.39945282604263	22.509358773095038	24.428426914465465
32	34.63448819240568	20.043116674354287	22.888064916528254	22.43433021671177
33	33.17984483540239	19.58067153527431	24.031705998919115	23.207777630404184
34	29.769707863744976	21.902795457109995	27.33514008959875	20.992356589546286
35	27.89320209604843	26.194468475138628	25.736180591161677	20.17614883765127
36	27.458471990988652	27.229031102207106	22.672481544756813	22.64001536204743
37	30.896521961194995	27.062542933557165	19.880389831749948	22.160545273497895
38	31.515755007017844	27.181717579844044	19.921368489194105	21.381158923944007
39	31.8722891232349	23.938464705893995	20.310764717177975	23.878481453693126
40	29.09148138836483	23.972910533890538	19.986498819141584	26.949109258603045
41	26.09370453002231	25.24423873730305	21.9128916480745	26.74916508460014
42	24.724581849424222	22.248045595190256	22.197762604896454	30.829609950489072
43	24.310242090822168	21.13904830532465	25.444380875834664	29.106328728018514
44	25.68767928162632	20.06509073704174	23.88303463785359	30.364195343478357
45	27.785113463369633	21.684638546465244	22.808087246927094	27.722160743238028
46	26.92990669931762	22.38780855246357	23.064847240671416	27.6174375075474
47	25.25829421884187	22.469369938294456	25.20939698024908	27.0629388626146
48	28.138480147127236	21.703049247635807	25.090024369433483	25.06844623580347
49	27.189042267406528	20.988199334443255	24.81722924886319	27.00552914928703
50	25.63739629133252	21.182798466170834	26.436381129229265	26.743424113267384
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	4.5
6	9.0
7	5.5
8	2.0
9	2.0
10	2.0
11	71.5
12	141.0
13	438.0
14	735.0
15	917.0
16	1099.0
17	1041.0
18	983.0
19	793.0
20	603.0
21	635.5
22	668.0
23	855.5
24	1043.0
25	1243.0
26	1443.0
27	1658.0
28	1873.0
29	2247.0
30	2621.0
31	2986.0
32	3351.0
33	4396.5
34	5442.0
35	5838.0
36	6234.0
37	6866.5
38	7499.0
39	8530.0
40	9561.0
41	10240.0
42	10919.0
43	11393.0
44	11867.0
45	13094.0
46	14321.0
47	15189.0
48	16057.0
49	17747.0
50	19437.0
51	22383.0
52	25329.0
53	26954.0
54	28579.0
55	30835.5
56	33092.0
57	38095.5
58	43099.0
59	49228.0
60	55357.0
61	56363.5
62	57370.0
63	54280.5
64	51191.0
65	42447.5
66	33704.0
67	26660.5
68	19617.0
69	16806.5
70	13996.0
71	11785.5
72	9575.0
73	7952.5
74	6330.0
75	6852.5
76	7375.0
77	5188.5
78	3002.0
79	1946.0
80	890.0
81	739.0
82	588.0
83	332.0
84	76.0
85	57.0
86	38.0
87	26.5
88	15.0
89	10.5
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	505141.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.031537729069704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.82911753101574	6.12601234110872
2	9.024155012932358	1.630039929445442
3	3.5596861163473763	0.9644831839031082
4	1.7842269080706679	0.6445725054984648
5	0.881153829292885	0.39790870271864687
6	0.6027793608346851	0.3266414723809788
7	0.3770110911402394	0.23834929257375664
8	0.3660514663977905	0.2644806103642349
9	0.315637192582526	0.2565620292156051
>10	8.693174345710403	23.178874809211685
>50	4.688527464819605	29.16136286700149
>100	1.7842269080706679	27.323856111461946
>500	0.061373898557713386	3.7543972870940987
>1k	0.03287887422734646	5.732458858021819
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4985	0.9868531756479874	No Hit
TGTCACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	3734	0.7391995502245907	No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	3198	0.6330905628329515	No Hit
TGTCACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2436	0.4822415919515541	No Hit
TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2325	0.46026752926410647	No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	1580	0.31278395537087667	No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	1491	0.29516511231517534	No Hit
TGTCACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	1281	0.25359256128486896	No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	1247	0.24686176730853363	No Hit
TGTCACGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCT	1213	0.24013097333219832	No Hit
TGTCACGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	1176	0.23280628576971577	No Hit
TGTCACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1151	0.22785717255182214	No Hit
TGTCACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1070	0.21182204572584687	No Hit
TGTCACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1065	0.2108322230822681	No Hit
TGTCACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1005	0.19895435135932343	No Hit
TGTCACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	983	0.19459913172757706	No Hit
TGTCACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	879	0.1740108207411396	No Hit
TGTCACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	872	0.1726250690401294	No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGTAAGAGCACCCGACTGT	866	0.17143728186783494	No Hit
TGTCACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	855	0.16925967205196174	No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	846	0.16747799129352003	No Hit
TGTCACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	835	0.16530038147764683	No Hit
TGTCACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	771	0.1526306516398392	No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTG	751	0.14867136106552428	No Hit
TGTCACGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTG	730	0.14451410596249364	No Hit
TGTCACGGGGGTTTTTCAGGACGCTTTGCAAGGGTAGCTGGGGTGAATTT	705	0.13956499274460002	No Hit
TGTCACGGGGCGTTTCCGTTGCACGGAGTTGTGAAGAGTACTGCCTTGTC	673	0.13323012782569618	No Hit
TGTCACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	667	0.13204234065340173	No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	650	0.12867694366523408	No Hit
TGTCACGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAA	645	0.12768712102165533	No Hit
TGTCACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	620	0.12273800780376173	No Hit
TGTCACGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	603	0.11937261081559405	No Hit
TGTCACGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	591	0.11699703647100512	No Hit
TGTCACGGGAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCC	582	0.11521535571256342	No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCT	570	0.11283978136797448	No Hit
TGTCACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	569	0.11264181683925874	No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGC	562	0.11125606513824854	No Hit
TGTCACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC	554	0.10967234890852257	No Hit
TGTCACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAC	529	0.10472323569062897	No Hit
TGTCACGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	528	0.10452527116191321	No Hit
TGTCACGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGC	520	0.10294155493218726	No Hit
TGTCACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	506	0.10017005153016682	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.009898226435787236	0.0	0.0	0.0	0.0
8	0.01346158795267064	0.0	0.0	0.0	0.0
9	0.01385751701010213	0.0	0.0	0.0	0.0
10	0.026131317790478302	0.0	0.0	0.0	0.0
11	0.032268218180666386	0.0	0.0	0.0	0.0
12	0.03820715404213873	0.0	0.0	0.0	0.0
13	0.04751148689177873	0.0	0.0	0.0	0.0
14	0.05325245822453533	0.0	0.0	0.0	0.0
15	0.06414050730390129	0.0	0.0	0.0	0.0
16	0.07245501750996257	0.0	0.0	0.0	0.0
17	0.07938377601501363	0.0	0.0	0.0	0.0
18	0.08750032169235916	0.0	0.0	0.0	0.0
19	0.0995761579440196	0.0	0.0	0.0	0.0
20	0.11244385231054299	0.0	0.0	0.0	0.0
21	0.1199665044017413	0.0	0.0	0.0	0.0
22	0.13045862442367576	0.0	0.0	0.0	0.0
23	0.1399609218020315	0.0	0.0	0.0	0.0
24	0.15381843881213364	0.0	0.0	0.0	0.0
25	0.1625288780756264	0.0	0.0	0.0	0.0
26	0.17203117545398217	0.0	0.0	0.0	0.0
27	0.18272126000463237	0.0	0.0	0.0	0.0
28	0.19420320267014557	0.0	0.0	0.0	0.0
29	0.20231974834749109	0.0	0.0	0.0	0.0
30	0.21182204572584684	0.0	0.0	0.0	0.0
31	0.22112637857548684	0.0	0.0	0.0	0.0
32	0.2306286759538426	0.0	0.0	0.0	0.0
33	0.24072486691834558	0.0	0.0	0.0	0.0
34	0.2488414125956911	0.0	0.0	0.0	0.0
35	0.26190707149093023	0.0	0.0	0.0	0.0
36	0.2743788368000222	0.0	0.0	0.0	0.0
37	0.28526688587938814	0.0	0.0	0.0	0.0
38	0.29635289948746985	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCAT	30	2.5248883E-6	44.000004	34
CGAACTA	30	2.5248883E-6	44.000004	40
ATTAACG	30	2.5248883E-6	44.000004	29
AGTGCGT	30	2.5248883E-6	44.000004	15
ATATACG	30	2.5248883E-6	44.000004	34
ACGTGGG	30	2.5248883E-6	44.000004	10
CCATAAC	30	2.5248883E-6	44.000004	34
TATTAAC	30	2.5248883E-6	44.000004	28
CGACTGA	30	2.5248883E-6	44.000004	44
TATACGG	30	2.5248883E-6	44.000004	35
ACGCGAA	20	7.852286E-4	44.0	25
CGGGTAT	55	1.8189894E-12	44.0	6
AAGTACG	20	7.852286E-4	44.0	38
AGCGTAC	25	4.439286E-5	44.0	36
AACCGAC	20	7.852286E-4	44.0	26
TCGCATA	20	7.852286E-4	44.0	25
CGTATTA	20	7.852286E-4	44.0	30
CATATAG	40	8.296411E-9	44.0	13
CGTATCG	25	4.439286E-5	44.0	28
GTATTAC	20	7.852286E-4	44.0	31
>>END_MODULE