FastQCFastQC Report
Fri 17 Jun 2016
SRR1527686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770104
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC75960.9863602838058236No Hit
TGTCACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC60320.7832708309527024No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT45590.5919979639113678No Hit
TGTCACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG36990.48032473536041886No Hit
TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT32410.42085224852747166No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT25220.32748823535522475No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA22160.28775334240570105No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC19250.24996623832625203No Hit
TGTCACGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCT18050.23438392736565453No Hit
TGTCACGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT17810.231267465173535No Hit
TGTCACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC17790.2310077599908584No Hit
TGTCACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC17750.2304883496255051No Hit
TGTCACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT16020.208023851323977No Hit
TGTCACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG15720.20412827358382762No Hit
TGTCACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC14660.19036389890196648No Hit
TGTCACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG13980.18153392269096122No Hit
TGTCACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTT13080.16984718947051308No Hit
TGTCACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC12810.16634116950437863No Hit
TGTCACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG12740.16543220136501044No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGTAAGAGCACCCGACTGTTC12670.16452323322564225No Hit
TGTCACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA12580.16335455990359743No Hit
TGTCACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG11210.1455647548902486No Hit
TGTCACGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT11180.14517519711623367No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCT10520.136604926087905No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT10510.1364750734965667No Hit
TGTCACGGGGGTTTTTCAGGACGCTTTGCAAGGGTAGCTGGGGTGAATTTGC9950.12920332838162119No Hit
TGTCACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA9840.12777494987689975No Hit
TGTCACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT9500.1233599617713971No Hit
TGTCACGGGGCGTTTCCGTTGCACGGAGTTGTGAAGAGTACTGCCTTGTCCT9450.12271069881470555No Hit
TGTCACGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT9380.12180173067533737No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT9050.11751659516117303No Hit
TGTCACGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAAT9040.11738674256983474No Hit
TGTCACGGGAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCAG8840.11478969074306847No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCTC8760.11375087001236196No Hit
TGTCACGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGCTC8720.11323145964700872No Hit
TGTCACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA8520.11063440782024246No Hit
TGTCACGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT8370.10868661895016778No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT8230.10686868267143139No Hit
TGTCACGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA8220.10673883008009309No Hit
TGTCACGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGG8100.10518059898403333No Hit
TGTCACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC8000.1038820730706502No Hit
TGTCACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG7940.10310295752262032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG551.8189894E-1246.00000443
TCACGGT1700.046.03
ACGCGAA253.4160985E-546.025
AACCGTA206.3101517E-446.026
CGGGTAT850.046.06
TAATAGA301.860597E-646.031
AACCGCG206.3101517E-446.027
CTGTCGG206.3101517E-446.024
AGTCCGT206.3101517E-446.040
CGTATTA206.3101517E-446.030
CATATAG405.6079443E-946.013
CGTATCG206.3101517E-446.028
ATTAGCG253.4160985E-546.031
GTATTAC206.3101517E-446.031
GGTAACG206.3101517E-446.040
CGAAACA206.3101517E-446.022
GCAACTA253.4160985E-546.023
ATAATCG301.860597E-646.027
ACCGATT253.4160985E-546.045
CGACCGA206.3101517E-446.040
Produced by FastQC (version 0.11.5)