##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527686_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	770104
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1977603025046	33.0	31.0	34.0	31.0	34.0
2	32.56465360522735	34.0	31.0	34.0	31.0	34.0
3	31.559939956161767	31.0	31.0	34.0	30.0	34.0
4	36.03583541963163	37.0	35.0	37.0	35.0	37.0
5	36.05842847199859	37.0	35.0	37.0	35.0	37.0
6	36.261369892897584	37.0	37.0	37.0	35.0	37.0
7	36.308642728774295	37.0	37.0	37.0	35.0	37.0
8	36.57020480350706	37.0	37.0	37.0	35.0	37.0
9	38.67551915066017	39.0	39.0	39.0	38.0	39.0
10	38.03855063731652	39.0	38.0	39.0	37.0	39.0
11	37.682581833103065	39.0	38.0	39.0	35.0	39.0
12	36.911757373030134	39.0	35.0	39.0	33.0	39.0
13	36.74000524604469	39.0	35.0	39.0	33.0	39.0
14	37.44112353656129	40.0	36.0	40.0	33.0	40.0
15	37.510492089380136	40.0	36.0	40.0	33.0	40.0
16	37.55457314856175	40.0	36.0	40.0	33.0	40.0
17	37.415984074878196	40.0	36.0	40.0	33.0	40.0
18	37.39088097192067	40.0	36.0	40.0	33.0	40.0
19	37.30445498270363	40.0	35.0	40.0	32.0	40.0
20	37.30180469131442	40.0	35.0	40.0	32.0	40.0
21	37.24814180941795	40.0	35.0	40.0	32.0	40.0
22	37.24717310908657	40.0	35.0	40.0	32.0	40.0
23	37.23718614628674	39.0	35.0	40.0	33.0	40.0
24	37.19759409118768	39.0	35.0	40.0	33.0	40.0
25	37.19524375928446	39.0	35.0	40.0	33.0	40.0
26	37.08709732711426	39.0	35.0	40.0	33.0	40.0
27	36.963984085266404	39.0	35.0	40.0	32.0	40.0
28	36.82200196337118	39.0	35.0	40.0	32.0	40.0
29	36.750577844031454	39.0	35.0	40.0	32.0	40.0
30	36.62550772363213	39.0	35.0	40.0	31.0	40.0
31	36.49672901322419	38.0	35.0	40.0	31.0	40.0
32	36.39066152104132	38.0	35.0	40.0	31.0	40.0
33	36.22840031995678	38.0	35.0	40.0	31.0	40.0
34	35.993777463823065	38.0	35.0	40.0	30.0	40.0
35	35.8812874624726	38.0	34.0	40.0	30.0	40.0
36	35.888646208823744	38.0	34.0	40.0	30.0	40.0
37	35.85128761829571	38.0	34.0	40.0	30.0	40.0
38	35.836023446183894	38.0	34.0	40.0	30.0	40.0
39	35.70358938532977	37.0	34.0	40.0	30.0	40.0
40	35.479337855666245	37.0	34.0	40.0	30.0	40.0
41	35.29049063503111	37.0	34.0	40.0	29.0	40.0
42	35.26385007739214	37.0	34.0	40.0	29.0	40.0
43	35.12078758193699	36.0	34.0	40.0	29.0	40.0
44	35.00646536052273	36.0	34.0	40.0	29.0	40.0
45	34.84958265377144	36.0	34.0	39.0	28.0	40.0
46	34.67612945783946	35.0	33.0	39.0	28.0	40.0
47	34.46562931759866	35.0	33.0	39.0	27.0	40.0
48	34.27703660804255	35.0	33.0	39.0	26.0	40.0
49	34.67401675617839	35.0	33.0	39.0	28.0	40.0
50	34.83753493034707	35.0	34.0	39.0	29.0	40.0
51	34.842049904947906	35.0	34.0	39.0	29.0	40.0
52	34.41438039537517	35.0	34.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	3.0
15	12.0
16	39.0
17	91.0
18	174.0
19	267.0
20	492.0
21	844.0
22	1323.0
23	2001.0
24	2847.0
25	3820.0
26	5269.0
27	7021.0
28	9036.0
29	11524.0
30	15050.0
31	19697.0
32	26100.0
33	37315.0
34	76774.0
35	71953.0
36	84393.0
37	126419.0
38	207096.0
39	60542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.45903410448459	0.4898039745281157	0.03012580119048856	0.021036119796806665
8	99.70770181689745	0.1923116877720412	0.046357375107777654	0.05362912022272316
9	98.37801128159313	0.8292386482864652	0.23931832583651036	0.553431744283889
10	60.94436595576701	29.253451481877775	3.292931863748273	6.509250698606942
11	41.12782169680978	23.219850825343073	19.49814570499569	16.15418177285146
12	35.542342332983594	14.868251560828147	23.703551728078285	25.88585437810997
13	27.52290599711208	15.426357998400217	28.21852632891142	28.832209675576287
14	26.362283535730242	17.471276606795964	30.870246096631103	25.29619376084269
15	29.729750786906706	17.817723320486582	28.601331768176763	23.85119412442995
16	34.16850191662425	18.857453019332453	23.774451242949006	23.199593821094293
17	32.95282195651497	20.666819027040503	22.91807340307283	23.4622856133717
18	33.011515327799884	20.70473598371129	22.056501459543128	24.2272472289457
19	30.19631114758526	22.771729532634552	22.89651787291067	24.135441446869514
20	33.14876951684448	22.39801377476289	22.370614877990505	22.082601830402126
21	31.90231449258801	22.791596979109315	23.72783416265855	21.57825436564412
22	30.173716796692396	20.50333461454557	25.36021108837248	23.962737500389558
23	30.533927885064877	22.292963028370195	24.2367264681134	22.936382618451535
24	31.110343538015645	19.83667141061467	23.566427391625027	25.48655765974466
25	29.99867550356835	21.42139243530744	22.229335258614423	26.35059680250979
26	27.601077257097746	20.688504409794	24.07181367711374	27.638604655994513
27	24.893261169919906	20.03989071605913	23.81561451440325	31.251233599617713
28	26.997262707374585	23.00922992219233	21.938725159199276	28.054782211233807
29	28.775853650935456	22.606167478678206	21.961838920457495	26.656139949928843
30	29.179045947040922	20.299076488370403	24.797300104920893	25.724577459667785
31	33.01008694929516	20.465807215648795	22.412037854627425	24.112067980428616
32	34.68077038945389	19.851734311209913	22.858730768831222	22.60876453050497
33	33.12734383927366	19.506066713067327	24.08791539843969	23.278674049219326
34	29.756630273313732	21.73083116046664	27.483300956753894	21.029237609465735
35	27.82533268233901	26.141534130455106	25.781972305039318	20.251160882166563
36	27.379938294048596	27.131919844592417	22.838733469765124	22.649408391593862
37	31.03671711872682	27.060111361582333	19.887184068645276	22.015987451045575
38	31.263439743203513	27.223206216303254	19.928736897873534	21.584617142619695
39	31.77311116420639	23.87651537974092	20.414255736887483	23.936117719165203
40	29.017509323416057	23.955335902683274	20.06767917060553	26.95947560329514
41	26.12309506248507	25.206076062453903	22.010923199983377	26.65990567507765
42	24.703286828791956	21.97858990474014	22.174147907295637	31.143975359172266
43	24.473447742123142	21.1274061685175	25.218152353448364	29.18099373591099
44	25.505905695854064	19.904064905519256	23.92027570302193	30.66975369560475
45	27.737941888368322	21.510211607782846	22.95339330791685	27.798453195931977
46	26.672111818663453	22.400740679180995	23.33996447233101	27.58718302982454
47	25.32904646645128	22.414634906454193	25.143357260837497	27.112961366257025
48	28.229563799175178	21.59136947736929	25.054148530588076	25.124918192867458
49	27.204507443150533	20.956909716090294	24.894819401015965	26.9437634397432
50	25.48655765974466	21.190384675316583	26.500576545505545	26.822481119433217
51	24.93156768436471	21.110135773869505	26.295019893416992	27.663276648348795
52	24.831061778668857	21.052870781089307	27.01648089089266	27.09958654934918
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	3.0
6	6.0
7	4.5
8	3.0
9	5.0
10	7.0
11	113.5
12	220.0
13	637.5
14	1218.5
15	1382.0
16	1391.5
17	1401.0
18	1147.5
19	894.0
20	895.5
21	897.0
22	1109.0
23	1321.0
24	1454.5
25	1588.0
26	1601.0
27	1614.0
28	2885.5
29	4157.0
30	4137.0
31	4117.0
32	5706.0
33	7295.0
34	7162.0
35	7029.0
36	8448.5
37	9868.0
38	11007.0
39	12746.5
40	13347.0
41	14996.5
42	16646.0
43	17978.0
44	19310.0
45	19351.0
46	19392.0
47	22231.0
48	25070.0
49	27034.0
50	28998.0
51	32620.5
52	36243.0
53	39556.0
54	42869.0
55	46110.5
56	49352.0
57	56845.0
58	64338.0
59	73465.5
60	82593.0
61	78848.5
62	75104.0
63	76917.0
64	68743.0
65	58756.0
66	45928.0
67	33100.0
68	28709.0
69	24318.0
70	20122.5
71	15927.0
72	12897.0
73	9867.0
74	11220.0
75	12573.0
76	8925.0
77	5277.0
78	3601.0
79	1925.0
80	1534.5
81	1144.0
82	646.5
83	149.0
84	88.5
85	28.0
86	27.5
87	27.0
88	22.0
89	10.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	770104.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.3640703073870535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.02422810389518	4.862070577480444
2	9.969847119606426	1.4683731028536406
3	4.503535469309305	0.9949305548341524
4	2.294087566785985	0.6757528853245796
5	1.333074712136975	0.4908427952588222
6	0.8675565586923171	0.38332484963069924
7	0.5713177337729893	0.2945056771552933
8	0.4108550369416868	0.24204523025461497
9	0.30329213027454993	0.20101181139170815
>10	5.568584577947841	11.475982464706066
>50	4.776851051824161	25.62082523918847
>100	3.2286505263529124	40.777349552787676
>500	0.10403625398952585	5.280455626772488
>1k	0.04055650579252702	5.4628985176028175
>5k	0.0035266526776110453	1.7696311147585262
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	7596	0.9863602838058236	No Hit
TGTCACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	6032	0.7832708309527024	No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4559	0.5919979639113678	No Hit
TGTCACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3699	0.48032473536041886	No Hit
TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3241	0.42085224852747166	No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	2522	0.32748823535522475	No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2216	0.28775334240570105	No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1925	0.24996623832625203	No Hit
TGTCACGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCT	1805	0.23438392736565453	No Hit
TGTCACGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT	1781	0.231267465173535	No Hit
TGTCACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1779	0.2310077599908584	No Hit
TGTCACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1775	0.2304883496255051	No Hit
TGTCACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1602	0.208023851323977	No Hit
TGTCACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1572	0.20412827358382762	No Hit
TGTCACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1466	0.19036389890196648	No Hit
TGTCACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1398	0.18153392269096122	No Hit
TGTCACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTT	1308	0.16984718947051308	No Hit
TGTCACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1281	0.16634116950437863	No Hit
TGTCACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	1274	0.16543220136501044	No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGTAAGAGCACCCGACTGTTC	1267	0.16452323322564225	No Hit
TGTCACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1258	0.16335455990359743	No Hit
TGTCACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1121	0.1455647548902486	No Hit
TGTCACGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	1118	0.14517519711623367	No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCT	1052	0.136604926087905	No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	1051	0.1364750734965667	No Hit
TGTCACGGGGGTTTTTCAGGACGCTTTGCAAGGGTAGCTGGGGTGAATTTGC	995	0.12920332838162119	No Hit
TGTCACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	984	0.12777494987689975	No Hit
TGTCACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	950	0.1233599617713971	No Hit
TGTCACGGGGCGTTTCCGTTGCACGGAGTTGTGAAGAGTACTGCCTTGTCCT	945	0.12271069881470555	No Hit
TGTCACGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	938	0.12180173067533737	No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	905	0.11751659516117303	No Hit
TGTCACGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAAT	904	0.11738674256983474	No Hit
TGTCACGGGAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCAG	884	0.11478969074306847	No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCTC	876	0.11375087001236196	No Hit
TGTCACGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGCTC	872	0.11323145964700872	No Hit
TGTCACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	852	0.11063440782024246	No Hit
TGTCACGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT	837	0.10868661895016778	No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT	823	0.10686868267143139	No Hit
TGTCACGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	822	0.10673883008009309	No Hit
TGTCACGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGG	810	0.10518059898403333	No Hit
TGTCACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	800	0.1038820730706502	No Hit
TGTCACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	794	0.10310295752262032	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.008180713254313703	0.0	0.0	0.0	0.0
8	0.009998649533050082	0.0	0.0	0.0	0.0
9	0.010647912489741646	0.0	0.0	0.0	0.0
10	0.01895847833539366	0.0	0.0	0.0	0.0
11	0.02584066567632424	0.0	0.0	0.0	0.0
12	0.03064521155584181	0.0	0.0	0.0	0.0
13	0.03765725148811069	0.0	0.0	0.0	0.0
14	0.044539438829041274	0.0	0.0	0.0	0.0
15	0.05103206839595691	0.0	0.0	0.0	0.0
16	0.06155012829436024	0.0	0.0	0.0	0.0
17	0.06986069414001225	0.0	0.0	0.0	0.0
18	0.07869067035101752	0.0	0.0	0.0	0.0
19	0.0906371087541423	0.0	0.0	0.0	0.0
20	0.10154472642656057	0.0	0.0	0.0	0.0
21	0.11011499745488922	0.0	0.0	0.0	0.0
22	0.11868526848321785	0.0	0.0	0.0	0.0
23	0.12959288615563613	0.0	0.0	0.0	0.0
24	0.13894227273199464	0.0	0.0	0.0	0.0
25	0.15062900595244277	0.0	0.0	0.0	0.0
26	0.16127691844218442	0.0	0.0	0.0	0.0
27	0.16945763169649813	0.0	0.0	0.0	0.0
28	0.17841746049884172	0.0	0.0	0.0	0.0
29	0.1873772893011853	0.0	0.0	0.0	0.0
30	0.1986744647476185	0.0	0.0	0.0	0.0
31	0.20659547281925558	0.0	0.0	0.0	0.0
32	0.218801616405057	0.0	0.0	0.0	0.0
33	0.22854056075543044	0.0	0.0	0.0	0.0
34	0.23879891547115714	0.0	0.0	0.0	0.0
35	0.2526931427443566	0.0	0.0	0.0	0.0
36	0.2661978122435411	0.0	0.0	0.0	0.0
37	0.2774949876899743	0.0	0.0	0.0	0.0
38	0.29061009941514393	0.0	0.0	0.0	0.0
39	0.30112815931354725	0.0	0.0	0.0	0.0
40	0.3120357769859655	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	55	1.8189894E-12	46.000004	43
TCACGGT	170	0.0	46.0	3
ACGCGAA	25	3.4160985E-5	46.0	25
AACCGTA	20	6.3101517E-4	46.0	26
CGGGTAT	85	0.0	46.0	6
TAATAGA	30	1.860597E-6	46.0	31
AACCGCG	20	6.3101517E-4	46.0	27
CTGTCGG	20	6.3101517E-4	46.0	24
AGTCCGT	20	6.3101517E-4	46.0	40
CGTATTA	20	6.3101517E-4	46.0	30
CATATAG	40	5.6079443E-9	46.0	13
CGTATCG	20	6.3101517E-4	46.0	28
ATTAGCG	25	3.4160985E-5	46.0	31
GTATTAC	20	6.3101517E-4	46.0	31
GGTAACG	20	6.3101517E-4	46.0	40
CGAAACA	20	6.3101517E-4	46.0	22
GCAACTA	25	3.4160985E-5	46.0	23
ATAATCG	30	1.860597E-6	46.0	27
ACCGATT	25	3.4160985E-5	46.0	45
CGACCGA	20	6.3101517E-4	46.0	40
>>END_MODULE