FastQCFastQC Report
Fri 17 Jun 2016
SRR1527685_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527685_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772365
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC70300.9101914250386799No Hit
TGTCACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC56750.7347562357175688No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT43430.562298913078661No Hit
TGTCACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG35820.4637703676370628No Hit
TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT31440.4070614282107553No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT24740.3203148770335269No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA20950.27124481300939324No Hit
TGTCACGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCT17770.23007256931632064No Hit
TGTCACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC17070.22100949680526694No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC17050.22075055187637968No Hit
TGTCACGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT16850.2181611025875072No Hit
TGTCACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC16180.20948644746978437No Hit
TGTCACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT15290.19796339813430178No Hit
TGTCACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC14690.19019505026768432No Hit
TGTCACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG14640.1895476879454662No Hit
TGTCACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG12940.16753736899005003No Hit
TGTCACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTT12680.16417108491451582No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGTAAGAGCACCCGACTGTTC12100.1566616819767856No Hit
TGTCACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG11920.15433117761680035No Hit
TGTCACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC11820.1530364529723641No Hit
TGTCACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA11630.15057647614793523No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCT10730.13892395434800903No Hit
TGTCACGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT10700.13853553695467816No Hit
TGTCACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG10310.13348611084137682No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT9800.12688301515475198No Hit
TGTCACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA9520.12325778615033048No Hit
TGTCACGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAAT9300.12040939193257075No Hit
TGTCACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT9280.12015044700368349No Hit
TGTCACGGGGCGTTTCCGTTGCACGGAGTTGTGAAGAGTACTGCCTTGTCCT9100.11781994264369824No Hit
TGTCACGGGGGTTTTTCAGGACGCTTTGCAAGGGTAGCTGGGGTGAATTTGC8930.11561891074815664No Hit
TGTCACGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT8660.11212315420817878No Hit
TGTCACGGGAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCAG8620.11160526435040427No Hit
TGTCACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA8550.1106989570992989No Hit
TGTCACGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT8470.10966317738374991No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCTC8300.10746214548820829No Hit
TGTCACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG8190.10603794837932842No Hit
TGTCACGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGCTC7960.10306008169712506No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT7820.10124746719491431No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT7730.1000822150149217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCA750.046.00000416
ACGCGAA206.310163E-446.025
AACGTGT453.092282E-1046.044
CGGGTAT1100.046.06
AGCGTAC301.8606042E-646.036
AGCGTAA206.310163E-446.026
GGTACGT206.310163E-446.031
ACCGGAT253.4161076E-546.040
CATATAG405.6079443E-946.013
CGTATCG253.4161076E-546.028
CGTATAG301.8606042E-646.015
ATCTACG206.310163E-446.026
ATAGGAT301.8606042E-646.024
TTTACGG206.310163E-446.013
CCGATTA900.046.010
ATAATCC206.310163E-446.039
GACCGTA253.4161076E-546.012
CACGCTA800.046.041
TTCGTTA551.8189894E-1246.042
CGTCTAG206.310163E-446.044
Produced by FastQC (version 0.11.5)