##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527685_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	772365
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.157174392935985	33.0	31.0	34.0	31.0	34.0
2	32.536924899497	34.0	31.0	34.0	31.0	34.0
3	31.53756967236993	31.0	31.0	33.0	30.0	34.0
4	36.02065733170198	37.0	35.0	37.0	35.0	37.0
5	36.07879694186039	37.0	35.0	37.0	35.0	37.0
6	36.22274183837952	37.0	37.0	37.0	35.0	37.0
7	36.27236216037754	37.0	35.0	37.0	35.0	37.0
8	36.54250516271452	37.0	37.0	37.0	35.0	37.0
9	38.63400205861218	39.0	39.0	39.0	38.0	39.0
10	37.952602720216476	39.0	38.0	39.0	35.0	39.0
11	37.65201815203952	39.0	38.0	39.0	35.0	39.0
12	36.862717756501134	39.0	35.0	39.0	33.0	39.0
13	36.54187204236339	39.0	35.0	39.0	33.0	39.0
14	37.388303457562166	40.0	36.0	40.0	33.0	40.0
15	37.47263923145145	40.0	36.0	40.0	33.0	40.0
16	37.548792345587906	40.0	36.0	40.0	33.0	40.0
17	37.452018152039514	40.0	36.0	40.0	33.0	40.0
18	37.43716118674461	40.0	36.0	40.0	33.0	40.0
19	37.29840943077431	40.0	35.0	40.0	32.0	40.0
20	37.304470036834914	40.0	35.0	40.0	32.0	40.0
21	37.31597754947467	40.0	35.0	40.0	33.0	40.0
22	37.193200106167424	40.0	35.0	40.0	32.0	40.0
23	37.21439215914755	39.0	35.0	40.0	32.0	40.0
24	37.22201938202793	39.0	35.0	40.0	33.0	40.0
25	37.189625371424135	39.0	35.0	40.0	33.0	40.0
26	37.0348242087614	39.0	35.0	40.0	33.0	40.0
27	36.915253798398425	39.0	35.0	40.0	32.0	40.0
28	36.791691751956655	39.0	35.0	40.0	32.0	40.0
29	36.70968389297806	39.0	35.0	40.0	32.0	40.0
30	36.57129207045892	38.0	35.0	40.0	31.0	40.0
31	36.466680908637755	38.0	35.0	40.0	31.0	40.0
32	36.363761951926875	38.0	35.0	40.0	31.0	40.0
33	36.02531445624802	38.0	35.0	40.0	30.0	40.0
34	35.90223922627255	38.0	34.0	40.0	30.0	40.0
35	35.85647459426566	38.0	34.0	40.0	30.0	40.0
36	35.7279537524357	38.0	34.0	40.0	30.0	40.0
37	35.757353064936915	38.0	34.0	40.0	30.0	40.0
38	35.48551138386644	37.0	34.0	40.0	29.0	40.0
39	35.503269827089525	37.0	34.0	40.0	29.0	40.0
40	35.360978293941336	37.0	34.0	40.0	29.0	40.0
41	34.97201193736122	37.0	34.0	40.0	28.0	40.0
42	35.09650877499628	36.0	34.0	40.0	29.0	40.0
43	35.07298362820687	36.0	34.0	40.0	29.0	40.0
44	34.965693681096376	36.0	34.0	40.0	29.0	40.0
45	34.80530707631755	35.0	33.0	39.0	28.0	40.0
46	34.453803577324194	35.0	33.0	39.0	27.0	40.0
47	34.16897321862073	35.0	33.0	39.0	26.0	40.0
48	34.13933308733565	35.0	33.0	39.0	26.0	40.0
49	34.08656270027772	35.0	33.0	39.0	26.0	40.0
50	34.00433603283422	35.0	33.0	39.0	27.0	40.0
51	34.387217183585484	35.0	33.0	39.0	28.0	40.0
52	34.198970693907675	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	11.0
16	31.0
17	78.0
18	154.0
19	322.0
20	524.0
21	857.0
22	1438.0
23	2067.0
24	3012.0
25	3995.0
26	5550.0
27	7380.0
28	9295.0
29	12005.0
30	15723.0
31	20415.0
32	27647.0
33	40723.0
34	78774.0
35	72051.0
36	87858.0
37	130790.0
38	200573.0
39	51089.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.45284936526124	0.4818965126591702	0.03702912483087659	0.02822499724871013
8	99.71192376661293	0.18074356036329975	0.04751639445081017	0.0598162785729545
9	98.38288891909913	0.8205964796436918	0.25557864481171466	0.540935956445463
10	60.893101059732125	29.224524674214912	3.3024541505635288	6.579920115489438
11	41.111521107248514	23.35191263198099	19.41957494189923	16.11699131887126
12	35.537602040486036	14.866157839881403	23.65824448285461	25.93799563677795
13	27.657778381982613	15.478174179306418	28.084260679859913	28.77978675885106
14	26.283428171913535	17.417153806814138	30.963987234015004	25.33543078725732
15	29.72156946521399	17.828228881422646	28.617816705832087	23.832384947531285
16	34.14162992885488	18.81001857929865	23.780595961753832	23.267755530092636
17	32.97223463000007	20.529024489716647	22.950806937134647	23.547933943148642
18	32.98207453729778	20.737475157470886	22.007211616269508	24.273238688961825
19	30.253053931755066	22.77588963767131	22.986541337321086	23.984515093252543
20	32.992302861988826	22.29761835401656	22.460365241822196	22.24971354217242
21	31.79079839195199	22.67917370673192	23.82979549824241	21.700232403073677
22	30.33073741042124	20.445385277686068	25.347601198915022	23.876276112977674
23	30.379807474445375	22.21618017388152	24.351051640092443	23.052960711580663
24	31.02341509519463	19.843467790487658	23.649440355272443	25.48367675904527
25	30.009127808743273	21.36852394916911	22.30409197723874	26.31825626484887
26	27.754494312922002	20.63545085548931	24.00186440348799	27.608190428100706
27	24.952321764968634	20.001553669573326	23.843519579473433	31.202604985984607
28	27.039806309193192	23.059822752196176	21.94817217248322	27.952198766127413
29	28.728904080324718	22.6927683154985	22.037378700484876	26.54094890369191
30	29.233069856868187	20.394502599159726	24.70710091731241	25.665326626659674
31	32.81712661759661	20.42751807759285	22.497782784046404	24.257572520764146
32	34.65356405326497	19.885028451574062	22.912483087659332	22.548924407501637
33	33.03891294918853	19.635793957520082	24.07009639224978	23.255196701041605
34	29.78449308293359	21.830481702303963	27.431460514135157	20.953564700627293
35	27.87671631935678	26.12417704064788	25.738866986463655	20.260239653531688
36	27.584108549714188	27.071009173124104	22.780421173926836	22.56446110323487
37	30.89238896117768	27.12668233283486	19.86379496740531	22.117133738582147
38	31.380500152130146	27.209415237614344	19.792585111961316	21.6174994982942
39	31.795588873136403	24.0066548846724	20.486816466308028	23.710939775883162
40	29.09090909090909	23.901005353686404	20.080920290277266	26.92716526512724
41	26.402283894272788	25.12154227599645	22.00151482783399	26.47465900189677
42	24.744777404465506	22.029092462760484	22.1668511649285	31.059278967845515
43	24.421484660749776	21.177552064114767	25.265904073851093	29.13505920128437
44	25.459465408194315	19.96814977374687	23.906572669657482	30.66581214840134
45	27.640299599282724	21.525832993468114	22.9091168035838	27.924750603665366
46	26.938429369533836	22.294640487334355	23.23849475312838	27.52843539000343
47	25.627520667042138	22.385271212444895	25.05110925533912	26.936098865173847
48	28.22072465738349	21.470030361292913	25.091504664245534	25.217740317078064
49	27.302246994620422	20.935697500534072	24.774038181429763	26.988017323415743
50	25.69018533983285	20.927540735274125	26.52062172677426	26.86165219811876
51	24.907135874877813	21.1708194959637	26.29313860674681	27.62890602241168
52	24.952451237433078	20.912910346791996	27.03035481928881	27.104283596486116
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	2.5
6	5.0
7	4.5
8	4.0
9	3.5
10	3.0
11	102.5
12	202.0
13	595.5
14	1173.0
15	1357.0
16	1372.0
17	1387.0
18	1156.0
19	925.0
20	903.0
21	881.0
22	1080.0
23	1279.0
24	1462.5
25	1646.0
26	1653.5
27	1661.0
28	2898.5
29	4136.0
30	4160.5
31	4185.0
32	5668.0
33	7151.0
34	7140.0
35	7129.0
36	8386.5
37	9644.0
38	10963.0
39	12832.0
40	13382.0
41	14962.5
42	16543.0
43	18072.0
44	19601.0
45	19527.0
46	19453.0
47	22398.5
48	25344.0
49	27082.0
50	28820.0
51	32523.5
52	36227.0
53	39389.5
54	42552.0
55	46367.5
56	50183.0
57	57505.0
58	64827.0
59	73531.5
60	82236.0
61	79179.5
62	76123.0
63	77430.0
64	68782.5
65	58828.0
66	45953.0
67	33078.0
68	28826.5
69	24575.0
70	20228.5
71	15882.0
72	13002.0
73	10122.0
74	11294.5
75	12467.0
76	8878.5
77	5290.0
78	3599.5
79	1909.0
80	1511.0
81	1113.0
82	627.5
83	142.0
84	85.0
85	28.0
86	25.5
87	23.0
88	18.0
89	6.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	772365.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.264751768917545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.08890293661284	8.233283486434523
2	10.062640078156427	2.2670628524078644
3	3.7273719901155107	1.2596376065720223
4	1.7918510430435033	0.8073902882704421
5	0.8861559680478134	0.4991163504301723
6	0.5988161599908051	0.40473092385077003
7	0.39078213895753117	0.3081444653758262
8	0.2827423711280961	0.2548018100250529
9	0.26090454571576344	0.26451224485832475
>10	3.802080340210333	11.994846995915145
>50	3.1239583931957933	25.64111527580872
>100	1.895293373944026	36.67372291597884
>500	0.06091603930808574	4.687550575181423
>1k	0.025285903109016725	5.059136548134625
>5k	0.0022987184644560656	1.6449476607562488
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	7030	0.9101914250386799	No Hit
TGTCACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5675	0.7347562357175688	No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4343	0.562298913078661	No Hit
TGTCACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3582	0.4637703676370628	No Hit
TGTCACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3144	0.4070614282107553	No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	2474	0.3203148770335269	No Hit
TGTCACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2095	0.27124481300939324	No Hit
TGTCACGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCT	1777	0.23007256931632064	No Hit
TGTCACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1707	0.22100949680526694	No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1705	0.22075055187637968	No Hit
TGTCACGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT	1685	0.2181611025875072	No Hit
TGTCACGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1618	0.20948644746978437	No Hit
TGTCACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1529	0.19796339813430178	No Hit
TGTCACGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1469	0.19019505026768432	No Hit
TGTCACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1464	0.1895476879454662	No Hit
TGTCACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1294	0.16753736899005003	No Hit
TGTCACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTT	1268	0.16417108491451582	No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGTGGTAAGAGCACCCGACTGTTC	1210	0.1566616819767856	No Hit
TGTCACGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	1192	0.15433117761680035	No Hit
TGTCACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1182	0.1530364529723641	No Hit
TGTCACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1163	0.15057647614793523	No Hit
TGTCACGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCT	1073	0.13892395434800903	No Hit
TGTCACGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	1070	0.13853553695467816	No Hit
TGTCACGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1031	0.13348611084137682	No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	980	0.12688301515475198	No Hit
TGTCACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	952	0.12325778615033048	No Hit
TGTCACGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAAT	930	0.12040939193257075	No Hit
TGTCACGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	928	0.12015044700368349	No Hit
TGTCACGGGGCGTTTCCGTTGCACGGAGTTGTGAAGAGTACTGCCTTGTCCT	910	0.11781994264369824	No Hit
TGTCACGGGGGTTTTTCAGGACGCTTTGCAAGGGTAGCTGGGGTGAATTTGC	893	0.11561891074815664	No Hit
TGTCACGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT	866	0.11212315420817878	No Hit
TGTCACGGGAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCAG	862	0.11160526435040427	No Hit
TGTCACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	855	0.1106989570992989	No Hit
TGTCACGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	847	0.10966317738374991	No Hit
TGTCACGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCTC	830	0.10746214548820829	No Hit
TGTCACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	819	0.10603794837932842	No Hit
TGTCACGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGCTC	796	0.10306008169712506	No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	782	0.10124746719491431	No Hit
TGTCACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT	773	0.1000822150149217	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.00699151307995572	0.0	0.0	0.0	0.0
8	0.009710434833271834	0.0	0.0	0.0	0.0
9	0.009969379762159083	0.0	0.0	0.0	0.0
10	0.01890297980876917	0.0	0.0	0.0	0.0
11	0.02731868999760476	0.0	0.0	0.0	0.0
12	0.03055550160869537	0.0	0.0	0.0	0.0
13	0.036770179901989344	0.0	0.0	0.0	0.0
14	0.043632220517501444	0.0	0.0	0.0	0.0
15	0.05088267852634441	0.0	0.0	0.0	0.0
16	0.06188783800405249	0.0	0.0	0.0	0.0
17	0.07108038297954983	0.0	0.0	0.0	0.0
18	0.07897820331061092	0.0	0.0	0.0	0.0
19	0.09127808743275524	0.0	0.0	0.0	0.0
20	0.09995274255047808	0.0	0.0	0.0	0.0
21	0.1084979252037573	0.0	0.0	0.0	0.0
22	0.1170431078570365	0.0	0.0	0.0	0.0
23	0.12636512529697747	0.0	0.0	0.0	0.0
24	0.13516925287914394	0.0	0.0	0.0	0.0
25	0.14643335728573925	0.0	0.0	0.0	0.0
26	0.15342487036569497	0.0	0.0	0.0	0.0
27	0.16119321823231245	0.0	0.0	0.0	0.0
28	0.17180996031668966	0.0	0.0	0.0	0.0
29	0.18113197775663062	0.0	0.0	0.0	0.0
30	0.18721718358548095	0.0	0.0	0.0	0.0
31	0.19666867348986555	0.0	0.0	0.0	0.0
32	0.20637910832313738	0.0	0.0	0.0	0.0
33	0.2138885112608676	0.0	0.0	0.0	0.0
34	0.22489367073857566	0.0	0.0	0.0	0.0
35	0.2373230273251636	0.0	0.0	0.0	0.0
36	0.25389550277394757	0.0	0.0	0.0	0.0
37	0.267878528933859	0.0	0.0	0.0	0.0
38	0.27784790869601805	0.0	0.0	0.0	0.0
39	0.28716992613595904	0.0	0.0	0.0	0.0
40	0.2973982508270054	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCA	75	0.0	46.000004	16
ACGCGAA	20	6.310163E-4	46.0	25
AACGTGT	45	3.092282E-10	46.0	44
CGGGTAT	110	0.0	46.0	6
AGCGTAC	30	1.8606042E-6	46.0	36
AGCGTAA	20	6.310163E-4	46.0	26
GGTACGT	20	6.310163E-4	46.0	31
ACCGGAT	25	3.4161076E-5	46.0	40
CATATAG	40	5.6079443E-9	46.0	13
CGTATCG	25	3.4161076E-5	46.0	28
CGTATAG	30	1.8606042E-6	46.0	15
ATCTACG	20	6.310163E-4	46.0	26
ATAGGAT	30	1.8606042E-6	46.0	24
TTTACGG	20	6.310163E-4	46.0	13
CCGATTA	90	0.0	46.0	10
ATAATCC	20	6.310163E-4	46.0	39
GACCGTA	25	3.4161076E-5	46.0	12
CACGCTA	80	0.0	46.0	41
TTCGTTA	55	1.8189894E-12	46.0	42
CGTCTAG	20	6.310163E-4	46.0	44
>>END_MODULE