FastQCFastQC Report
Fri 17 Jun 2016
SRR1527684_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527684_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113673
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC58560.5258275993042841No Hit
CCATCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51570.4630623172151969No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG40350.36231461120095393No Hit
CCATCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40140.3604289589493505No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT28930.2597710458994696No Hit
CCATCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC28770.25833435846967645No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG26210.23534735959298644No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC25140.22573951240624493No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT21230.1906304633406754No Hit
CCATCTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG17920.1609089921368301No Hit
CCATCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16760.15049300827082995No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGCAGTTAAGAGCACTGACTGCT16150.14501563744474366No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC15870.14250143444260568No Hit
CCATCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA14410.12939166164574342No Hit
CCATCTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG14280.12822435310903652No Hit
CCATCTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG13860.12445304860582954No Hit
CCATCTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC12730.11430644363291557No Hit
CCATCTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC12390.11125348284460519No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC12350.11089431098715691No Hit
CCATCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT12110.10873927984246723No Hit
CCATCTGGGAGCACATATCTCCTAACCTTTGAAGAATCTCCATTTTCTTT11880.1066740416621396No Hit
CCATCTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCATCTGGGA11460.10290273715893264No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGT207.8563753E-444.00000423
CGAAATA207.8563753E-444.00000424
TTTACGC207.8563753E-444.00000411
TAAACGA207.8563753E-444.00000419
ATTCGAT207.8563753E-444.00000424
CACGATA207.8563753E-444.00000436
TAACGGT207.8563753E-444.00000424
CGCTAGT207.8563753E-444.00000427
GTCACGC207.8563753E-444.00000416
AACGACC207.8563753E-444.00000432
AACGAAT207.8563753E-444.00000421
CGTACAT207.8563753E-444.00000436
TACTCGC207.8563753E-444.00000416
TTACGTC207.8563753E-444.00000419
TTACACG207.8563753E-444.00000414
GTAACGG207.8563753E-444.00000423
AATCGAA207.8563753E-444.00000435
TCCGATA551.8189894E-1244.025
CGGTTAG254.4427507E-544.018
CACGCTA351.4460238E-744.021
Produced by FastQC (version 0.11.5)