##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527684_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1113673
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.723516687573465	34.0	31.0	34.0	31.0	34.0
2	33.16826303591808	34.0	33.0	34.0	31.0	34.0
3	33.115020297699594	34.0	34.0	34.0	31.0	34.0
4	36.52074711338068	37.0	37.0	37.0	35.0	37.0
5	36.475908098696834	37.0	37.0	37.0	35.0	37.0
6	36.53970959159466	37.0	37.0	37.0	35.0	37.0
7	36.57136969289908	37.0	37.0	37.0	35.0	37.0
8	36.709337480571044	37.0	37.0	37.0	35.0	37.0
9	38.77287408422401	39.0	39.0	39.0	39.0	39.0
10	38.297238956138834	39.0	39.0	39.0	37.0	39.0
11	38.11247466715993	39.0	39.0	39.0	37.0	39.0
12	37.690655156405874	39.0	37.0	39.0	35.0	39.0
13	37.601698164542015	39.0	37.0	39.0	35.0	39.0
14	38.381050811144746	40.0	38.0	40.0	35.0	40.0
15	38.43375479157706	40.0	38.0	40.0	35.0	40.0
16	38.473797964034326	40.0	38.0	40.0	35.0	40.0
17	38.4007495916665	40.0	38.0	40.0	35.0	40.0
18	38.328586577927275	40.0	38.0	40.0	35.0	40.0
19	38.32163301076707	40.0	38.0	40.0	34.0	40.0
20	38.23939612435607	40.0	38.0	40.0	34.0	40.0
21	38.236158190061175	40.0	38.0	40.0	34.0	40.0
22	38.26170608428147	40.0	38.0	40.0	35.0	40.0
23	38.18757840048201	40.0	38.0	40.0	35.0	40.0
24	38.155510639119385	40.0	38.0	40.0	35.0	40.0
25	38.132009126556895	40.0	38.0	40.0	35.0	40.0
26	38.02253534026595	40.0	38.0	40.0	35.0	40.0
27	37.95259111067612	40.0	37.0	40.0	34.0	40.0
28	37.82488845469002	40.0	37.0	40.0	34.0	40.0
29	37.84477939215551	40.0	37.0	40.0	34.0	40.0
30	37.94756988810899	40.0	37.0	40.0	34.0	40.0
31	37.97385857428527	40.0	37.0	40.0	34.0	40.0
32	37.951763219544695	40.0	37.0	40.0	35.0	40.0
33	37.86922193498451	40.0	37.0	40.0	34.0	40.0
34	37.57355884537023	40.0	37.0	40.0	33.0	40.0
35	37.60569664524506	40.0	36.0	40.0	33.0	40.0
36	37.611810648188474	40.0	36.0	40.0	34.0	40.0
37	37.54159703970555	40.0	36.0	40.0	34.0	40.0
38	37.501842102663886	40.0	35.0	40.0	34.0	40.0
39	37.421494460223066	40.0	35.0	40.0	34.0	40.0
40	37.26366806055278	40.0	35.0	40.0	33.0	40.0
41	37.15902423781487	40.0	35.0	40.0	33.0	40.0
42	37.1176305791736	40.0	35.0	40.0	33.0	40.0
43	37.00674614541252	40.0	35.0	40.0	33.0	40.0
44	36.75579366654305	40.0	35.0	40.0	33.0	40.0
45	36.7355453530794	39.0	35.0	40.0	33.0	40.0
46	36.58637589310327	39.0	35.0	40.0	32.0	40.0
47	36.54973677192497	39.0	35.0	40.0	32.0	40.0
48	36.46875159943718	39.0	35.0	40.0	32.0	40.0
49	36.369677634278645	39.0	35.0	40.0	32.0	40.0
50	35.917212682717455	38.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	2.0
15	5.0
16	13.0
17	42.0
18	77.0
19	156.0
20	329.0
21	573.0
22	862.0
23	1353.0
24	2014.0
25	2819.0
26	3827.0
27	5104.0
28	6907.0
29	8923.0
30	11929.0
31	15767.0
32	20085.0
33	26891.0
34	53890.0
35	83102.0
36	82033.0
37	133745.0
38	335207.0
39	318016.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.38716301822888	0.46117666496359344	0.10290273715893264	0.048757579648604214
8	99.33391579036216	0.4896410346663697	0.08287890610619096	0.09356426886527734
9	97.78076688579142	1.3988845917966943	0.4028112381282477	0.41753728428362724
10	53.0297493070228	34.370591726655846	4.873423347787007	7.7262356185343455
11	34.374452824123416	22.72731762375491	24.96145637004758	17.93677318207409
12	30.480670717526596	18.029708900188833	27.314121829298188	24.17549855298638
13	24.754214208299924	18.16134538594363	28.390829264963774	28.693611140792676
14	23.96304839930572	20.026973806494368	28.998548047766263	27.011429746433645
15	25.05376353741179	23.498010636874557	27.128878943819235	24.319346881894415
16	30.156967081001333	22.578440888842596	25.197252694462378	22.067339335693692
17	29.806415348131814	22.598374926930976	22.871884296377843	24.72332542855937
18	28.423334318062842	23.404985125795452	24.873549057937115	23.29813149820459
19	29.629074243516722	23.78094826757944	22.28670354763023	24.303273941273606
20	30.1243722349379	24.57938730668697	22.649916088474804	22.64632436990032
21	30.359001250815993	22.69193919579625	24.00857343223729	22.940486121150464
22	27.047706104035925	22.856439906507568	25.353672038381102	24.742181951075406
23	26.57557469742016	24.574448693647057	25.373246904612035	23.476729704320746
24	27.512743866467087	25.534964033428125	23.776368826396975	23.175923273707813
25	26.206884785749494	26.07372181960055	22.380267816495504	25.339125578154448
26	25.27070333931055	27.309362802187	22.52276925093811	24.89716460756434
27	23.849550092352064	23.671670229950802	24.499830740262176	27.978948937434957
28	25.193391596994807	26.07506871406598	23.01537345342843	25.716166235510784
29	28.18771757957677	25.24277772739395	21.965154942249654	24.604349750779626
30	26.561477202015315	24.686510313170924	24.232696671285016	24.519315813528745
31	28.855777234430573	24.5814525448673	22.223579093683693	24.339191127018434
32	30.244066256432546	24.65607049825218	22.09203240089326	23.007830844422017
33	28.756196836953034	24.331379139118933	23.7460188044426	23.166405219485434
34	26.648037619660347	25.81960773045589	25.891531894909907	21.640822754973858
35	25.737716546957678	28.136176418032942	24.641164866168076	21.484942168841304
36	25.751185491611988	28.597891840782708	22.77553644561734	22.875386221987963
37	27.23276940358615	28.947006886222436	21.485121754770027	22.335101955421386
38	27.641955942184104	28.572211052975156	21.337861293216232	22.447971711624508
39	28.003193037812714	26.25214043978798	21.883982102466344	23.86068441993296
40	25.68877938138035	27.08263556717277	21.76150449907648	25.467080552370398
41	24.300041394556573	27.671767206352314	22.478680905436335	25.549510493654783
42	23.593819729848885	25.899523468738128	22.66114020902006	27.845516592392922
43	23.730394828643597	25.276899053851537	24.590701220196593	26.402004897308277
44	25.07899536039753	24.297257812661346	24.273193298212313	26.35055352872881
45	25.82301986310165	25.465284693083156	23.23563559500859	25.47605984880661
46	24.672771989623524	27.211578263996707	23.778164685684217	24.337485060695556
47	25.181000167912842	26.02496423995194	23.86849640783246	24.925539184302753
48	25.536670099751003	26.034212915281234	24.607133332674852	23.821983652292907
49	26.517299063549178	25.77273580305889	23.719799258848873	23.990165874543067
50	24.763373090664853	25.271870647847262	24.72763549084875	25.237120770639137
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	3.0
6	6.0
7	5.0
8	4.0
9	42.0
10	80.0
11	1674.5
12	3269.0
13	5534.0
14	7799.0
15	8609.5
16	9420.0
17	7482.5
18	5545.0
19	5084.0
20	4623.0
21	4673.0
22	4723.0
23	4792.5
24	4862.0
25	5240.0
26	5618.0
27	6425.5
28	7233.0
29	7965.0
30	8697.0
31	10027.5
32	11358.0
33	13296.5
34	15235.0
35	16495.0
36	17755.0
37	19580.5
38	21406.0
39	23852.0
40	26298.0
41	29437.5
42	32577.0
43	34346.5
44	36116.0
45	38177.0
46	40238.0
47	43151.0
48	46064.0
49	50411.0
50	54758.0
51	56747.0
52	58736.0
53	62583.0
54	66430.0
55	71887.0
56	77344.0
57	85129.5
58	92915.0
59	94809.5
60	96704.0
61	94385.0
62	92066.0
63	91573.0
64	91080.0
65	76898.5
66	62717.0
67	51473.0
68	40229.0
69	32130.0
70	24031.0
71	21167.0
72	18303.0
73	14567.0
74	10831.0
75	11265.5
76	11700.0
77	8361.5
78	5023.0
79	3057.0
80	1091.0
81	748.5
82	406.0
83	369.0
84	332.0
85	176.5
86	21.0
87	24.5
88	28.0
89	14.0
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1113673.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.018563898915936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.34721606717898	6.073751715159516
2	9.26304674471979	1.6707875793180136
3	3.444857201917348	0.9320299439439705
4	1.6339087881929752	0.5894204324527461
5	0.8799505673816392	0.3967945209909323
6	0.6119656165766809	0.3311430610221771
7	0.43297567307454343	0.2733373142009234
8	0.3629796058319722	0.2618843815359168
9	0.31298241494598583	0.2540386717584655
>10	9.233481212815411	24.523975333337656
>50	4.444750269760072	27.501737825870315
>100	1.9548901636402567	29.17341509189327
>500	0.05199707852137767	3.1871617810747823
>1k	0.02299870780753243	3.837363706306777
>5k	0.001999887635437603	0.9931586411345305
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5856	0.5258275993042841	No Hit
CCATCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5157	0.4630623172151969	No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	4035	0.36231461120095393	No Hit
CCATCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4014	0.3604289589493505	No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2893	0.2597710458994696	No Hit
CCATCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2877	0.25833435846967645	No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	2621	0.23534735959298644	No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	2514	0.22573951240624493	No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	2123	0.1906304633406754	No Hit
CCATCTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1792	0.1609089921368301	No Hit
CCATCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1676	0.15049300827082995	No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGCAGTTAAGAGCACTGACTGCT	1615	0.14501563744474366	No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	1587	0.14250143444260568	No Hit
CCATCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1441	0.12939166164574342	No Hit
CCATCTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1428	0.12822435310903652	No Hit
CCATCTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	1386	0.12445304860582954	No Hit
CCATCTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	1273	0.11430644363291557	No Hit
CCATCTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	1239	0.11125348284460519	No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	1235	0.11089431098715691	No Hit
CCATCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	1211	0.10873927984246723	No Hit
CCATCTGGGAGCACATATCTCCTAACCTTTGAAGAATCTCCATTTTCTTT	1188	0.1066740416621396	No Hit
CCATCTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCATCTGGGA	1146	0.10290273715893264	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.005926335647896645	0.0	0.0	0.0	0.0
8	0.008620124578758756	0.0	0.0	0.0	0.0
9	0.010146604972913953	0.0	0.0	0.0	0.0
10	0.02621954559372455	0.0	0.0	0.0	0.0
11	0.04283124400070757	0.0	0.0	0.0	0.0
12	0.05216971229436289	0.0	0.0	0.0	0.0
13	0.05935314944332852	0.0	0.0	0.0	0.0
14	0.07111602777475974	0.0	0.0	0.0	0.0
15	0.08521352317960479	0.0	0.0	0.0	0.0
16	0.1024537723371223	0.0	0.0	0.0	0.0
17	0.11852671295793289	0.0	0.0	0.0	0.0
18	0.1324446224340538	0.0	0.0	8.979296436207038E-5	0.0
19	0.14573398115964023	0.0	0.0	8.979296436207038E-5	0.0
20	0.1627048514240715	0.0	0.0	8.979296436207038E-5	0.0
21	0.17509628050603723	0.0	0.0	8.979296436207038E-5	0.0
22	0.1897325336970547	0.0	0.0	8.979296436207038E-5	0.0
23	0.20733195471202048	0.0	0.0	8.979296436207038E-5	0.0
24	0.22663744204986563	0.0	0.0	8.979296436207038E-5	0.0
25	0.24163286709833137	0.0	0.0	8.979296436207038E-5	0.0
26	0.2633627644739524	0.0	0.0	8.979296436207038E-5	0.0
27	0.2813213573463665	0.0	0.0	8.979296436207038E-5	0.0
28	0.3104142777996773	0.0	0.0	8.979296436207038E-5	0.0
29	0.32918100735135	0.0	0.0	8.979296436207038E-5	0.0
30	0.34992318211898826	0.0	0.0	8.979296436207038E-5	0.0
31	0.36357171270202293	0.0	0.0	8.979296436207038E-5	0.0
32	0.3826078211467819	0.0	0.0	8.979296436207038E-5	0.0
33	0.39814200398142	0.0	0.0	8.979296436207038E-5	0.0
34	0.4174474913192652	0.0	0.0	8.979296436207038E-5	0.0
35	0.4428589002337311	0.0	0.0	8.979296436207038E-5	0.0
36	0.4620745946072142	0.0	0.0	8.979296436207038E-5	0.0
37	0.4804821523014386	0.0	0.0	8.979296436207038E-5	0.0
38	0.4995182607461975	0.0	0.0	8.979296436207038E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCGT	20	7.8563753E-4	44.000004	23
CGAAATA	20	7.8563753E-4	44.000004	24
TTTACGC	20	7.8563753E-4	44.000004	11
TAAACGA	20	7.8563753E-4	44.000004	19
ATTCGAT	20	7.8563753E-4	44.000004	24
CACGATA	20	7.8563753E-4	44.000004	36
TAACGGT	20	7.8563753E-4	44.000004	24
CGCTAGT	20	7.8563753E-4	44.000004	27
GTCACGC	20	7.8563753E-4	44.000004	16
AACGACC	20	7.8563753E-4	44.000004	32
AACGAAT	20	7.8563753E-4	44.000004	21
CGTACAT	20	7.8563753E-4	44.000004	36
TACTCGC	20	7.8563753E-4	44.000004	16
TTACGTC	20	7.8563753E-4	44.000004	19
TTACACG	20	7.8563753E-4	44.000004	14
GTAACGG	20	7.8563753E-4	44.000004	23
AATCGAA	20	7.8563753E-4	44.000004	35
TCCGATA	55	1.8189894E-12	44.0	25
CGGTTAG	25	4.4427507E-5	44.0	18
CACGCTA	35	1.4460238E-7	44.0	21
>>END_MODULE