FastQCFastQC Report
Fri 17 Jun 2016
SRR1527683_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527683_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1665807
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC90980.5461617102101264No Hit
CCATCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70830.4251993178081255No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT60620.36390770359351354No Hit
CCATCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53270.31978494507466954No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG44900.2695390282307614No Hit
CCATCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT44310.26599720135645966No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT39540.23736243154218947No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC39470.23694221479439095No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC31420.1886172887975618No Hit
CCATCTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG25610.15373929873028508No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT24450.146775706909624No Hit
CCATCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23880.14335394196326465No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA22910.13753093845805667No Hit
CCATCTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC21300.12786595325869082No Hit
CCATCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC20990.12600499337558313No Hit
CCATCTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC20920.1255847766277846No Hit
CCATCTGGGAGCACATATCTCCTAACCTTTGAAGAATCTCCATTTTCTTTTT19070.11447904829310959No Hit
CCATCTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC18580.11153753105851998No Hit
CCATCTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA18570.11147750009454878No Hit
CCATCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT18160.10901623057172889No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC17490.10499415598565741No Hit
CCATCTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG17080.10253288646283752No Hit
CCATCTGGGGGGGCTGGAGAGACAGCTCAGTGGTTAAGAGCACTGACTGCTC16970.10187254585915416No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATA206.312275E-446.024
TCCGATA600.046.025
TAGGTCG206.312275E-446.033
CGTTATA253.4178214E-546.023
ACGATCT301.8619103E-646.026
ACGGCTA650.046.022
CTACGTT253.4178214E-546.034
TTGCGTA206.312275E-446.020
TTAACCG301.8619103E-646.032
CGTACAC301.8619103E-646.044
TCGTACT253.4178214E-546.029
TCGTAAC453.110472E-1046.010
CGTAATT253.4178214E-546.040
CATAACG253.4178214E-546.022
ACGTAGG253.4178214E-546.043
CGCAATT206.312275E-446.044
CGCAATA253.4178214E-546.029
ATCGGAT206.312275E-446.043
GACGAAC206.312275E-446.036
ATTGCCG301.8619103E-646.044
Produced by FastQC (version 0.11.5)