##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527683_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1665807
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40991843592925	33.0	31.0	34.0	31.0	34.0
2	32.858696715765994	34.0	31.0	34.0	31.0	34.0
3	32.8143086203864	34.0	31.0	34.0	31.0	34.0
4	36.36327617785253	37.0	37.0	37.0	35.0	37.0
5	36.203878960767966	37.0	37.0	37.0	35.0	37.0
6	36.36585210651654	37.0	37.0	37.0	35.0	37.0
7	36.49192973735853	37.0	37.0	37.0	35.0	37.0
8	36.63364303307646	37.0	37.0	37.0	35.0	37.0
9	38.70935348452732	39.0	39.0	39.0	39.0	39.0
10	38.09867229516985	39.0	38.0	39.0	37.0	39.0
11	37.80701545857353	39.0	38.0	39.0	35.0	39.0
12	37.35884169054398	39.0	37.0	39.0	35.0	39.0
13	37.2629110094987	39.0	37.0	39.0	34.0	39.0
14	38.06365443295652	40.0	38.0	40.0	34.0	40.0
15	38.13281730716704	40.0	38.0	40.0	34.0	40.0
16	38.17144122938612	40.0	38.0	40.0	35.0	40.0
17	38.09498339243382	40.0	38.0	40.0	34.0	40.0
18	38.089111163538156	40.0	38.0	40.0	34.0	40.0
19	38.08324433742925	40.0	38.0	40.0	34.0	40.0
20	38.06518042006067	40.0	38.0	40.0	34.0	40.0
21	38.04317306866882	40.0	38.0	40.0	34.0	40.0
22	38.029170246012896	40.0	38.0	40.0	34.0	40.0
23	37.9997142526115	40.0	38.0	40.0	34.0	40.0
24	37.958261071060456	40.0	37.0	40.0	34.0	40.0
25	37.930349074052394	40.0	37.0	40.0	34.0	40.0
26	37.79347007186307	40.0	37.0	40.0	34.0	40.0
27	37.718893005011985	40.0	37.0	40.0	34.0	40.0
28	37.63210684070844	40.0	36.0	40.0	34.0	40.0
29	37.56346503526519	40.0	36.0	40.0	33.0	40.0
30	37.429330048438985	40.0	36.0	40.0	33.0	40.0
31	37.355998624090304	40.0	36.0	40.0	33.0	40.0
32	37.25938539098467	40.0	35.0	40.0	33.0	40.0
33	37.14924718169632	39.0	35.0	40.0	33.0	40.0
34	36.97839965854388	39.0	35.0	40.0	32.0	40.0
35	36.91169625292726	39.0	35.0	40.0	32.0	40.0
36	36.95825326703514	39.0	35.0	40.0	32.0	40.0
37	36.86628042744447	39.0	35.0	40.0	32.0	40.0
38	36.83815892237216	39.0	35.0	40.0	32.0	40.0
39	36.74189266823828	39.0	35.0	40.0	32.0	40.0
40	36.55925086159441	39.0	35.0	40.0	31.0	40.0
41	36.45120893356793	39.0	35.0	40.0	31.0	40.0
42	36.409646495662464	39.0	35.0	40.0	31.0	40.0
43	36.279857150318136	38.0	35.0	40.0	31.0	40.0
44	36.178041633874756	38.0	35.0	40.0	31.0	40.0
45	36.068506735774314	38.0	35.0	40.0	30.0	40.0
46	35.933084084770925	38.0	35.0	40.0	30.0	40.0
47	35.79762661580843	38.0	35.0	40.0	30.0	40.0
48	35.62153718888202	38.0	34.0	40.0	29.0	40.0
49	35.86950048835189	38.0	35.0	40.0	30.0	40.0
50	35.921088097240556	38.0	35.0	40.0	30.0	40.0
51	35.936774188126236	38.0	35.0	40.0	31.0	40.0
52	35.47919296773276	37.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	8.0
16	23.0
17	53.0
18	126.0
19	281.0
20	597.0
21	992.0
22	1746.0
23	2701.0
24	4100.0
25	5880.0
26	8045.0
27	11237.0
28	14533.0
29	18668.0
30	23840.0
31	30505.0
32	40236.0
33	56045.0
34	104838.0
35	127469.0
36	150649.0
37	226718.0
38	515514.0
39	320999.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.46722519475546	0.4343240243317503	0.06735474157570476	0.031096039337090074
8	99.3908057776201	0.45851650281215045	0.06597402940436677	0.08470369016338627
9	97.80706888613146	1.3755495084364515	0.3917620708761579	0.42561953455592394
10	53.04576100352561	34.40752740263428	4.833933342818226	7.712778251021877
11	34.45189028500901	22.699808561255896	24.828866729459055	18.019434424276042
12	30.566686296791882	17.915160639858037	27.350827556853822	24.16732550649625
13	24.856060756138014	17.938752808698727	28.378317536185165	28.82686889897809
14	23.962860043210288	19.844615852856904	29.10595285048028	27.08657125345253
15	25.262710506079035	23.273824638748668	27.107702152770397	24.355762702401897
16	30.342410615395423	22.394190923678433	25.175425484464885	22.08797297646126
17	29.94050331160813	22.45212080391066	22.884043589683557	24.723332294797657
18	28.567295010766554	23.218536120931176	24.93698249557122	23.277186372731055
19	29.71070478152631	23.466223878276416	22.344185130690413	24.478886209506864
20	30.084757718030957	24.40919026033628	22.788654387933295	22.717397633699463
21	30.474538767096064	22.439634363404647	24.036337943111057	23.049488926388232
22	27.017235490065776	22.69302506232715	25.350355713477015	24.939383734130065
23	26.785275845281	24.184134176408193	25.50565581727055	23.524934161040264
24	27.612262404948474	25.243920814356045	23.81050145665134	23.333315324044143
25	26.27543286827346	25.78407942816905	22.412140181905826	25.528347521651668
26	25.41915119818803	26.95660421645485	22.459504612479115	25.16473997287801
27	23.94557112558658	23.316086437384403	24.492753362184214	28.24558907484481
28	25.261750010655497	25.836726583571807	23.015631462708466	25.88589194306423
29	28.288211059264366	25.051821729648154	21.96827123430265	24.691695976784825
30	26.774170116946323	24.351740627815825	24.23167869987339	24.642410555364457
31	28.988892470736406	24.223874674557138	22.188945057860845	24.598287796845614
32	30.455749075373078	24.33451174115609	22.131255301484508	23.07848388198633
33	28.948311539091865	23.96292007417426	23.796994489757818	23.29177389697606
34	26.631716639442626	25.502954423891843	25.9756382342012	21.889690702464332
35	25.79644580674712	27.901491589361793	24.782342732381363	21.519719871509725
36	25.896517423687136	28.34067812177521	22.832116805848457	22.930687648689194
37	27.33780083767207	28.60901653072655	21.53544798407018	22.517734647531196
38	27.85652839734735	28.259876444269956	21.367421315914747	22.516173842467946
39	28.056191383515618	26.050676939165225	21.894913396329827	23.998218280989335
40	25.73917626711858	26.716960608281752	21.86543819301996	25.67842493157971
41	24.34447688117531	27.344584336600818	22.563958489789034	25.74698029243484
42	23.651719556947473	25.601105049984785	22.75767841052415	27.989496982543592
43	23.851202450223827	24.947367852338235	24.616657271820806	26.584772425617132
44	25.080996778138164	23.895085084886787	24.511903239691033	26.51201489728402
45	25.90053949827321	25.025708260320673	23.210792126578887	25.862960114827228
46	24.593905536475713	26.982657654818354	23.905770596473662	24.51766621223227
47	25.316978497509012	25.593120931776607	23.959858495011726	25.130042075702647
48	25.691871867509263	25.600804895164924	24.747344680386142	23.95997855693967
49	26.743374232429083	25.31445719702222	23.83025164379787	24.11191692675082
50	24.882354318357407	24.880313265582387	24.856000725174045	25.38133169088616
51	23.443111957147497	25.215586199361628	25.202379387287966	26.138922456202913
52	24.97264088817012	24.637968264030587	25.081477025849935	25.307913821949363
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	4.0
6	8.0
7	8.0
8	8.0
9	63.0
10	118.0
11	2314.0
12	4510.0
13	7611.5
14	11631.0
15	12549.0
16	9852.5
17	7156.0
18	6594.5
19	6033.0
20	5794.0
21	5555.0
22	5817.0
23	6079.0
24	6496.5
25	6914.0
26	7836.5
27	8759.0
28	9977.0
29	11195.0
30	12675.0
31	14155.0
32	16185.5
33	18216.0
34	20203.0
35	22190.0
36	24857.0
37	27524.0
38	30217.5
39	37926.5
40	42942.0
41	44471.0
42	46000.0
43	48691.5
44	51383.0
45	54734.5
46	58086.0
47	64269.5
48	70453.0
49	71951.0
50	73449.0
51	80020.5
52	86592.0
53	92014.0
54	97436.0
55	104342.5
56	111249.0
57	122025.0
58	132801.0
59	139227.0
60	145653.0
61	136501.5
62	127350.0
63	129939.5
64	116898.0
65	101267.0
66	86774.5
67	72282.0
68	56163.0
69	40044.0
70	33901.5
71	27759.0
72	24573.0
73	21387.0
74	19675.0
75	17963.0
76	14199.5
77	10436.0
78	6549.0
79	2662.0
80	1721.5
81	781.0
82	658.0
83	535.0
84	349.5
85	164.0
86	85.0
87	6.0
88	4.5
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1665807.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.697437240535292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.93135558404173	5.228973462726479
2	9.557789510218663	1.4714096982630942
3	4.126704699855179	0.9529515131207182
4	2.127852468452159	0.655160433321144
5	1.199252912258232	0.461558701381846
6	0.7315958818198177	0.33788480314452735
7	0.5087701599700417	0.2741358463358548
8	0.36980519589655725	0.22772418293100874
9	0.3097139134084039	0.21456030698835935
>10	5.162811568146366	11.536127154112597
>50	4.765730050786905	26.534793514143924
>100	3.083799458300971	41.283477948143656
>500	0.08815313402175809	4.452750276453203
>1k	0.03354499790208494	4.712942408126951
>5k	0.0031204649211241803	1.6555497508066515
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	9098	0.5461617102101264	No Hit
CCATCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7083	0.4251993178081255	No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	6062	0.36390770359351354	No Hit
CCATCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5327	0.31978494507466954	No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4490	0.2695390282307614	No Hit
CCATCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4431	0.26599720135645966	No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3954	0.23736243154218947	No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	3947	0.23694221479439095	No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3142	0.1886172887975618	No Hit
CCATCTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2561	0.15373929873028508	No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT	2445	0.146775706909624	No Hit
CCATCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2388	0.14335394196326465	No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2291	0.13753093845805667	No Hit
CCATCTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	2130	0.12786595325869082	No Hit
CCATCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2099	0.12600499337558313	No Hit
CCATCTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2092	0.1255847766277846	No Hit
CCATCTGGGAGCACATATCTCCTAACCTTTGAAGAATCTCCATTTTCTTTTT	1907	0.11447904829310959	No Hit
CCATCTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1858	0.11153753105851998	No Hit
CCATCTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1857	0.11147750009454878	No Hit
CCATCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1816	0.10901623057172889	No Hit
CCATCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	1749	0.10499415598565741	No Hit
CCATCTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1708	0.10253288646283752	No Hit
CCATCTGGGGGGGCTGGAGAGACAGCTCAGTGGTTAAGAGCACTGACTGCTC	1697	0.10187254585915416	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.005042600973582174	0.0	0.0	0.0	0.0
8	0.007984118208171775	0.0	0.0	0.0	0.0
9	0.008644458811855155	0.0	0.0	0.0	0.0
10	0.02317195209288951	0.0	0.0	0.0	0.0
11	0.038299755013636035	0.0	0.0	0.0	0.0
12	0.04760455442917457	0.0	0.0	0.0	0.0
13	0.055408579745432694	0.0	0.0	0.0	0.0
14	0.06603406036833799	0.0	0.0	0.0	0.0
15	0.07786016027066761	0.0	0.0	0.0	0.0
16	0.09106697234433521	0.0	0.0	0.0	0.0
17	0.10565449658934079	0.0	0.0	0.0	0.0
18	0.11934155637477811	0.0	0.0	0.0	0.0
19	0.1343492973675822	0.0	0.0	0.0	0.0
20	0.14923697643244385	0.0	0.0	0.0	0.0
21	0.16130320019065833	0.0	0.0	0.0	0.0
22	0.17499025997609566	0.0	0.0	0.0	0.0
23	0.19329970398731666	0.0	0.0	0.0	0.0
24	0.20932797136763143	0.0	0.0	0.0	0.0
25	0.22445577428837796	0.0	0.0	0.0	0.0
26	0.24732757156141139	0.0	0.0	0.0	0.0
27	0.26593717039248843	0.0	0.0	0.0	0.0
28	0.2911501752603993	0.0	0.0	0.0	0.0
29	0.30597782336128976	0.0	0.0	0.0	0.0
30	0.32470748412030925	0.0	0.0	0.0	0.0
31	0.3392349774013436	0.0	0.0	0.0	0.0
32	0.3555033686375432	0.0	0.0	0.0	0.0
33	0.3714716050538868	0.0	0.0	0.0	0.0
34	0.3902012658129063	0.0	0.0	0.0	0.0
35	0.4149340229690474	0.0	0.0	0.0	0.0
36	0.43360365276409574	0.0	0.0	0.0	0.0
37	0.4497519820723529	0.0	0.0	0.0	0.0
38	0.4665006210203223	0.0	0.0	0.0	0.0
39	0.48072795948150054	0.0	0.0	0.0	0.0
40	0.49459511215885155	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAATA	20	6.312275E-4	46.0	24
TCCGATA	60	0.0	46.0	25
TAGGTCG	20	6.312275E-4	46.0	33
CGTTATA	25	3.4178214E-5	46.0	23
ACGATCT	30	1.8619103E-6	46.0	26
ACGGCTA	65	0.0	46.0	22
CTACGTT	25	3.4178214E-5	46.0	34
TTGCGTA	20	6.312275E-4	46.0	20
TTAACCG	30	1.8619103E-6	46.0	32
CGTACAC	30	1.8619103E-6	46.0	44
TCGTACT	25	3.4178214E-5	46.0	29
TCGTAAC	45	3.110472E-10	46.0	10
CGTAATT	25	3.4178214E-5	46.0	40
CATAACG	25	3.4178214E-5	46.0	22
ACGTAGG	25	3.4178214E-5	46.0	43
CGCAATT	20	6.312275E-4	46.0	44
CGCAATA	25	3.4178214E-5	46.0	29
ATCGGAT	20	6.312275E-4	46.0	43
GACGAAC	20	6.312275E-4	46.0	36
ATTGCCG	30	1.8619103E-6	46.0	44
>>END_MODULE