FastQCFastQC Report
Fri 17 Jun 2016
SRR1527682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676030
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC84030.5013633407516571No Hit
CCATCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69090.41222412486649995No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT56300.335912841655579No Hit
CCATCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51090.3048274792217323No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG43330.25852759198821024No Hit
CCATCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT42210.2518451340369802No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT37500.2237430117599327No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC37030.22093876601254153No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC29410.17547418602292322No Hit
CCATCTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG24830.14814770618664344No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT23380.13949630973192603No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA22340.1332911702057839No Hit
CCATCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21460.1280406675298175No Hit
CCATCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC20940.12493809776674641No Hit
CCATCTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC20730.12368513690089081No Hit
CCATCTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC20230.1207018967440917No Hit
CCATCTGGGAGCACATATCTCCTAACCTTTGAAGAATCTCCATTTTCTTTTT18790.11211016509251029No Hit
CCATCTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC18170.10841094729807939No Hit
CCATCTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA17690.10554703674755224No Hit
CCATCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT17450.10411508147228868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG405.6134013E-946.041
AAATCGC301.8619176E-646.023
CGAAATA206.312287E-446.024
CCGATTA253.417831E-546.019
CGTGATA206.312287E-446.042
ACCGATA301.8619176E-646.040
CGATTAT253.417831E-546.020
GCGCATT206.312287E-446.012
CGTTATA206.312287E-446.023
CTACGTT301.8619176E-646.034
CGCTAAT206.312287E-446.043
ATTGCCG253.417831E-546.044
ATCGCGC253.417831E-546.021
TCGGTAA206.312287E-446.026
ATCGATC650.046.043
TCCGATA351.01994374E-745.99999625
ATACGTT351.01994374E-745.99999623
ATCTGGG1705500.043.809913
CATCTGG1744950.043.7025682
CCATCTG1756150.043.666141
Produced by FastQC (version 0.11.5)