##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527682_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1676030
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35834740428274	33.0	31.0	34.0	31.0	34.0
2	32.82671849549232	34.0	31.0	34.0	31.0	34.0
3	32.81154334946272	34.0	31.0	34.0	31.0	34.0
4	36.363532275675254	37.0	37.0	37.0	35.0	37.0
5	36.22134031013765	37.0	37.0	37.0	35.0	37.0
6	36.31072415171566	37.0	37.0	37.0	35.0	37.0
7	36.44805820898194	37.0	37.0	37.0	35.0	37.0
8	36.60663591940478	37.0	37.0	37.0	35.0	37.0
9	38.66643377505176	39.0	39.0	39.0	38.0	39.0
10	38.007160969672384	39.0	38.0	39.0	37.0	39.0
11	37.77508218826632	39.0	38.0	39.0	35.0	39.0
12	37.31229094944601	39.0	37.0	39.0	35.0	39.0
13	37.071264237513645	39.0	37.0	39.0	34.0	39.0
14	38.002686706085214	40.0	38.0	40.0	34.0	40.0
15	38.09288735881816	40.0	38.0	40.0	34.0	40.0
16	38.15670781549256	40.0	38.0	40.0	35.0	40.0
17	38.11555461417755	40.0	38.0	40.0	34.0	40.0
18	38.11477419855253	40.0	38.0	40.0	34.0	40.0
19	38.06925711353615	40.0	38.0	40.0	34.0	40.0
20	38.06258121871327	40.0	38.0	40.0	34.0	40.0
21	38.09971718883313	40.0	38.0	40.0	34.0	40.0
22	37.983451370202204	40.0	38.0	40.0	34.0	40.0
23	37.97500402737421	40.0	38.0	40.0	34.0	40.0
24	37.974224208397224	40.0	37.0	40.0	34.0	40.0
25	37.920145224130835	40.0	37.0	40.0	34.0	40.0
26	37.735139585806934	40.0	37.0	40.0	34.0	40.0
27	37.67235252352285	40.0	37.0	40.0	34.0	40.0
28	37.608153791996564	40.0	36.0	40.0	34.0	40.0
29	37.530102086478166	40.0	36.0	40.0	33.0	40.0
30	37.393284726407046	40.0	36.0	40.0	33.0	40.0
31	37.340299994630165	40.0	36.0	40.0	33.0	40.0
32	37.24459765040005	40.0	35.0	40.0	33.0	40.0
33	36.94535241015972	39.0	35.0	40.0	32.0	40.0
34	36.891235837067356	39.0	35.0	40.0	31.0	40.0
35	36.90835724897526	39.0	35.0	40.0	32.0	40.0
36	36.79759073524937	39.0	35.0	40.0	32.0	40.0
37	36.78804734998777	39.0	35.0	40.0	32.0	40.0
38	36.49708418107075	39.0	35.0	40.0	31.0	40.0
39	36.580498559095005	39.0	35.0	40.0	31.0	40.0
40	36.47137461739945	39.0	35.0	40.0	31.0	40.0
41	36.164432020906546	38.0	35.0	40.0	30.0	40.0
42	36.267559053238905	38.0	35.0	40.0	31.0	40.0
43	36.244827956540156	38.0	35.0	40.0	31.0	40.0
44	36.13344987858213	38.0	35.0	40.0	31.0	40.0
45	36.03121364176059	38.0	35.0	40.0	30.0	40.0
46	35.72120666097862	38.0	35.0	40.0	30.0	40.0
47	35.50344683567717	38.0	34.0	40.0	29.0	40.0
48	35.48972154436377	38.0	34.0	40.0	29.0	40.0
49	35.42114401293533	37.0	34.0	40.0	29.0	40.0
50	35.30670990376067	37.0	34.0	40.0	29.0	40.0
51	35.604206368621085	37.0	35.0	40.0	30.0	40.0
52	35.33422074783864	37.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	3.0
16	19.0
17	61.0
18	117.0
19	278.0
20	629.0
21	1040.0
22	1818.0
23	2864.0
24	4460.0
25	6257.0
26	8277.0
27	11557.0
28	15460.0
29	19066.0
30	25070.0
31	31901.0
32	42087.0
33	59480.0
34	109053.0
35	128827.0
36	160009.0
37	244357.0
38	521163.0
39	282176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.4481602357953	0.448798649188857	0.07219441179453828	0.030846703221302722
8	99.37733811447289	0.47236624642756997	0.0681372051812915	0.08215843391824729
9	97.77629278712195	1.3776603044098257	0.4144317225825313	0.4316151858856942
10	53.01116328466674	34.45385822449479	4.87318246093447	7.661796029904
11	34.43452682827873	22.704486196547794	24.82002112134031	18.040965853833164
12	30.52487127318723	17.885360047254526	27.38393704169973	24.20583163785851
13	25.07902603175361	17.9231278676396	28.293825289523454	28.704020811083335
14	23.9315525378424	19.883474639475427	29.084264601468945	27.100708221213228
15	25.204799436764258	23.305549423339677	27.122127885539044	24.367523254357025
16	30.368370494561553	22.321020506792838	25.17836792897502	22.13224106967059
17	29.881267041759397	22.484502067385428	22.889566415875613	24.744664474979565
18	28.562257238832238	23.243020709653166	24.963992291307434	23.230729760207154
19	29.733298329982162	23.464436794090798	22.44900150951952	24.353263366407525
20	30.050178099437364	24.38965889632047	22.787718596922492	22.77244440731968
21	30.4535121686366	22.441782068340068	24.031491083095172	23.073214679928164
22	27.196291235837066	22.63980954994839	25.2327225646319	24.931176649582646
23	26.764198731526285	24.254935770839424	25.41016568915831	23.570699808475982
24	27.615973461095567	25.268879435332302	23.80070762456519	23.31443947900694
25	26.223516285508015	25.745004564357437	22.416663186219818	25.61481596391473
26	25.4708447939476	26.914017052200734	22.527639720052743	25.087498433798917
27	24.019259798452293	23.245705625794287	24.46423990024045	28.270794675512967
28	25.18737731424855	25.797628920723376	23.07995680268253	25.935036962345542
29	28.304922942906753	25.00265508373955	22.0025894524561	24.689832520897596
30	26.783052809317255	24.38757062821071	24.187932196917718	24.641444365554317
31	28.961116447796282	24.167168845426396	22.272095368221333	24.599619338555993
32	30.426663007225407	24.316390518069486	22.158612912656693	23.098333562048413
33	28.93629588969171	23.97504817932853	23.828988741251646	23.25966718972811
34	26.66521482312369	25.429198761358684	25.94088411305287	21.964702302464755
35	25.784264004820916	27.922471554805107	24.794245926385567	21.499018513988414
36	26.156870700405122	28.268646742600072	22.728292452999053	22.846190103995752
37	27.26043089920825	28.64364003030972	21.52640465862783	22.569524411854204
38	27.894071108512374	28.231535235049492	21.297351479388794	22.577042177049336
39	28.0259303234429	26.04810176428823	21.912734258933313	24.01323365333556
40	25.796614619070063	26.73878152539036	21.803309009981923	25.66129484555766
41	24.587089729897436	27.269798273300598	22.580144746812408	25.56296724998956
42	23.748858910640024	25.533970155665468	22.71904440851297	27.998126525181533
43	23.863653991873655	24.90558044903731	24.591982243754586	26.638783315334454
44	25.09173463482157	23.953151196577625	24.50415565353842	26.45095851506238
45	25.946134615728834	24.96751251469246	23.241409759968498	25.844943109610206
46	24.785534865127712	26.837526774580407	23.910968180760488	24.465970179531393
47	25.478183564733325	25.649481214536735	23.908521923831913	24.963813296898028
48	25.69124657673192	25.63838356115344	24.732373525533554	23.937996336581087
49	26.804591803249345	25.25879608360232	23.78113756913659	24.155474544011742
50	24.941737319737715	24.870079891171397	24.80403095409987	25.38415183499102
51	23.42535634803673	25.222519883295647	25.194596755428005	26.15752701323962
52	25.00205843570819	24.58303252328419	25.058978657899917	25.3559303831077
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	2.0
6	4.0
7	5.0
8	6.0
9	68.5
10	131.0
11	2220.0
12	4309.0
13	7428.5
14	11561.5
15	12575.0
16	9910.0
17	7245.0
18	6695.5
19	6146.0
20	5831.5
21	5517.0
22	5742.5
23	5968.0
24	6558.5
25	7149.0
26	7904.5
27	8660.0
28	10006.0
29	11352.0
30	12734.5
31	14117.0
32	16123.0
33	18129.0
34	20407.0
35	22685.0
36	25027.5
37	27370.0
38	29961.0
39	37655.0
40	42758.0
41	44484.5
42	46211.0
43	49021.5
44	51832.0
45	55243.0
46	58654.0
47	64971.5
48	71289.0
49	73135.5
50	74982.0
51	80728.0
52	86474.0
53	92441.5
54	98409.0
55	105491.5
56	112574.0
57	122868.5
58	133163.0
59	139867.0
60	146571.0
61	137873.0
62	129175.0
63	130790.5
64	117293.5
65	102181.0
66	87371.5
67	72562.0
68	56281.0
69	40000.0
70	34163.5
71	28327.0
72	24949.0
73	21571.0
74	19591.5
75	17612.0
76	14109.5
77	10607.0
78	6668.0
79	2729.0
80	1767.0
81	805.0
82	649.0
83	493.0
84	327.0
85	161.0
86	87.0
87	13.0
88	9.5
89	3.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1676030.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.498116425473578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.67586128057613	6.712875589135322
2	9.985130984840843	1.8967987323524393
3	3.8715619785902	1.1031763926325968
4	1.9225429657173092	0.7304214768543305
5	1.067982143864837	0.5071909371377548
6	0.6478492656328434	0.3692008650682983
7	0.4231037028472053	0.28130817607842123
8	0.3003611097031549	0.2282291831716005
9	0.23341905996911028	0.19953372667600874
>10	4.52828708223423	12.509185202507314
>50	3.8602127019570083	26.321253214183937
>100	2.3958381712782035	39.326625221140965
>500	0.058781686057243605	3.7243311566850443
>1k	0.026484056355461402	4.491554541268121
>5k	0.0025838103761425756	1.5983155851078548
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	8403	0.5013633407516571	No Hit
CCATCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6909	0.41222412486649995	No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5630	0.335912841655579	No Hit
CCATCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5109	0.3048274792217323	No Hit
CCATCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4333	0.25852759198821024	No Hit
CCATCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4221	0.2518451340369802	No Hit
CCATCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3750	0.2237430117599327	No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	3703	0.22093876601254153	No Hit
CCATCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2941	0.17547418602292322	No Hit
CCATCTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2483	0.14814770618664344	No Hit
CCATCTGGGGGGCTGGTGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT	2338	0.13949630973192603	No Hit
CCATCTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2234	0.1332911702057839	No Hit
CCATCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2146	0.1280406675298175	No Hit
CCATCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2094	0.12493809776674641	No Hit
CCATCTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2073	0.12368513690089081	No Hit
CCATCTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	2023	0.1207018967440917	No Hit
CCATCTGGGAGCACATATCTCCTAACCTTTGAAGAATCTCCATTTTCTTTTT	1879	0.11211016509251029	No Hit
CCATCTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1817	0.10841094729807939	No Hit
CCATCTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1769	0.10554703674755224	No Hit
CCATCTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1745	0.10411508147228868	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.005608491494782313	0.0	0.0	0.0	0.0
8	0.008353072439037488	0.0	0.0	0.0	0.0
9	0.009188379682941237	0.0	0.0	0.0	0.0
10	0.021658323538361484	0.0	0.0	0.0	0.0
11	0.036574524322357	0.0	0.0	0.0	0.0
12	0.044808267155122525	0.0	0.0	0.0	0.0
13	0.052087373137712334	0.0	0.0	0.0	0.0
14	0.06181273604887741	0.0	0.0	0.0	0.0
15	0.07416335029802569	0.0	0.0	0.0	0.0
16	0.0871106125785338	0.0	0.0	0.0	0.0
17	0.10262346139388913	0.0	0.0	0.0	0.0
18	0.11646569572143697	0.0	0.0	0.0	0.0
19	0.13006927083644088	0.0	0.0	0.0	0.0
20	0.1452837956361163	0.0	0.0	0.0	0.0
21	0.15542681216923324	0.0	0.0	0.0	0.0
22	0.1675984320089736	0.0	0.0	0.0	0.0
23	0.18424491208391258	0.0	0.0	0.0	0.0
24	0.19987709050553987	0.0	0.0	0.0	0.0
25	0.2147932912895354	0.0	0.0	0.0	0.0
26	0.2373465868749366	0.0	0.0	0.0	0.0
27	0.2532770893122438	0.0	0.0	0.0	0.0
28	0.28042457473911564	0.0	0.0	0.0	0.0
29	0.2969517252077827	0.0	0.0	0.0	0.0
30	0.3151494901642572	0.0	0.0	0.0	0.0
31	0.32917071890121297	0.0	0.0	0.0	0.0
32	0.3462348525981038	0.0	0.0	0.0	0.0
33	0.3603157461381956	0.0	0.0	0.0	0.0
34	0.37827485188212623	0.0	0.0	0.0	0.0
35	0.40405004683687046	0.0	0.0	0.0	0.0
36	0.4215318341557132	0.0	0.0	0.0	0.0
37	0.43805898462438025	0.0	0.0	0.0	0.0
38	0.45327350942405564	0.0	0.0	0.0	0.0
39	0.46824937501118713	0.0	0.0	0.0	0.0
40	0.482031944535599	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	40	5.6134013E-9	46.0	41
AAATCGC	30	1.8619176E-6	46.0	23
CGAAATA	20	6.312287E-4	46.0	24
CCGATTA	25	3.417831E-5	46.0	19
CGTGATA	20	6.312287E-4	46.0	42
ACCGATA	30	1.8619176E-6	46.0	40
CGATTAT	25	3.417831E-5	46.0	20
GCGCATT	20	6.312287E-4	46.0	12
CGTTATA	20	6.312287E-4	46.0	23
CTACGTT	30	1.8619176E-6	46.0	34
CGCTAAT	20	6.312287E-4	46.0	43
ATTGCCG	25	3.417831E-5	46.0	44
ATCGCGC	25	3.417831E-5	46.0	21
TCGGTAA	20	6.312287E-4	46.0	26
ATCGATC	65	0.0	46.0	43
TCCGATA	35	1.01994374E-7	45.999996	25
ATACGTT	35	1.01994374E-7	45.999996	23
ATCTGGG	170550	0.0	43.80991	3
CATCTGG	174495	0.0	43.702568	2
CCATCTG	175615	0.0	43.66614	1
>>END_MODULE