FastQCFastQC Report
Fri 17 Jun 2016
SRR1527681_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527681_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19383
Sequences flagged as poor quality0
Sequence length50
%GC58

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCTGGGCTATCTGTCCTCTAGTCCCACCACTCCCTGGCCAGGTCAT4322.228757158334623No Hit
CGTGGCCGGGACAAGCTATCGGACTGTGTGCTGCCATCGGTAATGAGTCT3351.7283186297270805No Hit
CGTGGCGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC2141.1040602589898365No Hit
CGTGGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT2141.1040602589898365No Hit
CGTGGCTCTGTACTCTCCACTATACTCCTGCTGCTACCTCCACCGCCACT2111.088582778723624No Hit
CGTGGCCTGATCCCACCCTGGGCACAGCCCATTGAGCCATCCTGCCACGC2091.0782644585461487No Hit
CGTGGCGGCATTTCTATCTGTACTGGGCTCAGAAGGCACAGTGGCCATAT2031.0473094980137234No Hit
CGTGGCCTTATGGTTACAGTATGCCCATCGCAGTTCGCTACACGCAGGAC1810.9338079760614971No Hit
CGTGGCGGGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.8048289738430584No Hit
CGTGGCGAGCCCCCAACTACCGCCTGCATGTGGACGGTGGTCCTCACGGT1520.7841923334881082No Hit
CGTGGCCTTGCTATTGACTCTACTGTAGACATTTTTACTTTTTATGTCCC1350.6964866119795697No Hit
CGTGGCGAGCAATGGCTGCTGCCAAGGCCCCCACAAAGGCAGCACCTAAA1310.6758499716246196No Hit
CGTGGCGGGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.6139400505597689No Hit
CGTGGCGGGCTGTGCAGTCTCAGTCACCCTCCACAAACTGGTCACCTGCA1090.5623484496723934No Hit
CGTGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1020.5262343290512305No Hit
CGTGGCGAGCTTCTTAAACCTGAAGTGGAGTACTACTCTGGTTGCGCTTG1020.5262343290512305No Hit
CGTGGCGAGAGAGGAGAATGAAGAGGCCAAGAGTGGAGAAGACTAGAAGG940.4849610483413301No Hit
CGTGGCGGAGTGACTCTTTGGCCTCTCTTTGGCCATCCTGGTGAACAGCA900.4643244079863798No Hit
CGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA880.4540060878089047No Hit
CGTGGCGAGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC870.44884692772016715No Hit
CGTGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.438528607542692No Hit
CGTGGCCCAGTCAGCTGACCAGCTAGAATCACATATTTATTTTCCATTTG850.438528607542692No Hit
CGTGGCGGTGCCAGTCTAGAGCTCCTGGAAGGTAAAGTCCTTCCTGGGGT830.42821028736521694No Hit
CGTGGCGGTAATTGGACTTTGCATGTTACAAAAAGAGGAGGTCACAATGT770.39725532683279163No Hit
CGTGGCGGGATCAAGGCATATGTCACCCGGTCCCCCGTGGATGCAGGCAA690.3559820461228912No Hit
CGTGGCGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGT660.34050456585667854No Hit
CGTGGCGGGGTCTGCGCCGAGAAACCGAAGTGTAGGTGACGGTTCCGAGA660.34050456585667854No Hit
CGTGGCGGCGCCCCTTTTCAAATACATGGACAATGATTACGCTAAGCTGG650.335345405767941No Hit
CGTGGCGGCACCCACATCTTAGAGCTGCAGAAGCCCAGCCTGACTCCGCT650.335345405767941No Hit
CGTGGCCGGAGTGGAGACTGAGTTTGAAATAAACCGTTACAATATAAAAA640.33018624567920346No Hit
CGTGGCAGGGTTCTCCTGGCTCCACTTTCTTGGAGAAATAAATGATATAG620.3198679255017283No Hit
CGTGGCAGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAA620.3198679255017283No Hit
CGTGGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT610.31470876541299075No Hit
CGTGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.31470876541299075No Hit
CGTGGCGTGGCGTGGTGGGGTTGGACTGTCCATCCAGCTGGGGCACTCTC610.31470876541299075No Hit
CGTGGCGGGACCTCGCTCCCAGACACCACAAGATACCAACAGAGCTGAAA570.2940721250580406No Hit
CGTGGCGGTGGGAGGACTGAAGGAAGATGGGTCAAAGCCAGAGTGGTGGC560.288912964969303No Hit
CGTGGCGGCGGACCGAGCCGGTGCCGGGGGAAGAGCCTGCTGAGGGATGC560.288912964969303No Hit
CGTGGCGAGAGAGGAGTTTCAACGGATGCAGACTCAGCTCCTGGAGCTCC540.2785946447918279No Hit
CGTGGCGGAGAGGCATCGCACAAATGTTGGAAAAGTACCAGCAGGGAGAC540.2785946447918279No Hit
CGTGGCAGCTCTTCTCACCTCTGCATGCACTTAACTTTGGCATTGGTGGT540.2785946447918279No Hit
CGTGGCGGGACGCAGGATTACATCTTCTTCAGTTCCTAGAGTCCTCTAGA520.2682763246143528No Hit
CGTGGCGGACTTTCAAACAATATGATTGGGAGCTTTAGAGCTAATATTAG520.2682763246143528No Hit
CGTGGCGGGGCCGCCGCCATGGCCGACGTGGAGGACGGCGAGGAACCCTG510.26311716452561523No Hit
CGTGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.25795800443687766No Hit
CGTGGCGAGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.25795800443687766No Hit
CGTGGCGAGGAGCCGGAAGTCCCGCCTGTGGAGTAGTCGTCGCTGCCGCC500.25795800443687766No Hit
CGTGGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA480.24763968425940258Illumina Single End Adapter 2 (97% over 34bp)
CGTGGCGGGACGTAAGACAGAAGAAATGGTGTTTTGCCCATTAAATCAAG480.24763968425940258No Hit
CGTGGCGGGATCTCCATTAAAACTTGCCTTAAGCTTCCAGATTGCTTTGC470.24248052417066504No Hit
CGTGGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATCTCCATCTGGT460.23732136408192747No Hit
CGTGGCGGGATCCAAAACAAACAAACAAACAAACAAAAGTATTTTGAAGT440.22700304390445236No Hit
CGTGGCGAGGCGCGTGGGTCCTAAAGTGGTGTTTCGTGGGTTATCTTTGC430.2218438838157148No Hit
CGTGGCGGGACCAAGAAAGGGAAAGAAAGAACTAAGACAAAAAAAAAAAA430.2218438838157148No Hit
CGTGGCGAGTCACGAGGGCGAGGTGAGCTGAGGTGGCCAGCTTTAGGAAA420.21668472372697722No Hit
CGTGGCGGTGGCTGAAACAGGCTTCAGGACGGGCTGCTTTGGGAGACCTC410.21152556363823968No Hit
CGTGGCGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGGAGTGCAGCGG410.21152556363823968No Hit
CGTGGCGGGGGCAAAGTAAATGCTCACCCGCATCAAAGTGCAACGGGACG410.21152556363823968No Hit
CGTGGCGGGGCGCAGTTTGAATCGCGGTCCGACGGAGGAGTGGGCGCTGG410.21152556363823968No Hit
CGTGGCGGGGTCCAGCAGCCGTTGAAAAATGGCGACTGTTACGGAACTGA410.21152556363823968No Hit
CGTGGCGGAATTAAGGAATTAAATTTTAGAATAAACAATTTGAATAAAGA400.20636640354950211No Hit
CGTGGCGGGACTCAGGGTCCTCTTCCTCAGATGAAGACACAAGCAAGGAA380.19604808337202706No Hit
CGTGGCGAGATTCTGGGTCCTCCAGGGTCTGTGTATGAAGGTGGAGTATT370.1908889232832895No Hit
CGTGGCGGGGCTCCAGCTCCGCGTCCCGAAGGTGGGTCCCACGCGGGGTC360.18572976319455192No Hit
CGTGGCGGGGCTTCCACAGGAGGCCTACACGCCGCCGTCTGTGCCGCCGC350.18057060310581438No Hit
CGTGGCGGGATCCTATACTGTCCCCGAGCCAGGGTCTATAAACCTGACAC350.18057060310581438No Hit
CGTGGCGGCCTTTCGGAGCTGTGCGGCATTCTGAGCAGGAATGGCAGTGT330.17025228292833927No Hit
CGTGGCGGGCAGCAGTGTCTGCCCTGGAGCTGGGTTGCCCCGTAGTGGAT320.16509312283960173No Hit
CGTGGCGGGGCTTAGCAGTCTGCTTACCTGTCTACCCACTGCTGGAAGTT320.16509312283960173No Hit
CGTGGCGGAGCAAAATTGAAACTGCTGTCTCTGAAACTCTTCCTCCATTA320.16509312283960173No Hit
CGTGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.15993396275086416No Hit
CGTGGCGGGATCCAACAGTACTTCCTGTACCTGTGTATAGGTTTTTGTGG310.15993396275086416No Hit
CGTGGCGGAACAAATGTTGGTGAAAAGAAAAGGCCTGTAGCCACACGGTC310.15993396275086416No Hit
CGTGGCGGGCTCCCAGAACCACGACGCCGTATTTCCTTTGAGGCCTTTGC300.1547748026621266No Hit
CGTGGCGGGATCTGCGTCAATAAGAGGCAGTAGCAGCAGAGACAGCGGCT300.1547748026621266No Hit
CGTGGCGGCGCACTGCAGTGTCTGCGACAACTGTGTGGAACGGTTTGACC290.14961564257338905No Hit
CGTGGCGGGGGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.14961564257338905No Hit
CGTGGCGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAG290.14961564257338905No Hit
CGTGGCGGGGGCTAAGTTCCGGGGGCAGGGCGGCCACTGGAGGAGAGCAA280.1444564824846515No Hit
CGTGGCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA280.1444564824846515Illumina Single End Adapter 2 (97% over 34bp)
CGTGGCGGGCAGTAGATATCCTTGGGCCCTCTCCAAGCCTGGGCCTTTTC280.1444564824846515No Hit
CGTGGCGGGCTGCAGACTCAGTGCCTTACTCAGACTCCTCTCTCGCTCTC270.13929732239591394No Hit
CGTGGCGGGATGTAAAGACCTATCTGCTACCAGACAAAGGCAAAATGGGC270.13929732239591394No Hit
CGTGGCGGGCTCTTCGGTTCTCATCGCTGTGCGGACGCCAGAGCCGAGCC270.13929732239591394No Hit
CGTGGCGGGACCGCCGTACGCAATGCCCTTCAGCCTAGAGCGCGGAGGAG260.1341381623071764No Hit
CGTGGCGGGACATCTAAGCTGCAGCAAGCAGCAGCAGCAGCAGCAGCAAG260.1341381623071764No Hit
CGTGGCGGGACTCTGCAGACTCAGTGCCTTACTCAGACTCCTCTCTCGCT260.1341381623071764No Hit
CGTGGCTCTGATTTTCTTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.1341381623071764No Hit
CGTGGCGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCT250.12897900221843883No Hit
CGTGGCGGGATCTACAGAGCTGCCTACTTCGGAGTCTATGACACTGCCAA250.12897900221843883No Hit
CGTGGCAGGCTGCTTCCCTCAAGAGTAACTATAACCTGCCCATGCACAAG250.12897900221843883No Hit
CGTGGCTGGCCTGCCTTCCTCCTGCCTCCCTTCCTTCCTTCCTTCTCAAT250.12897900221843883No Hit
CGTGGCGGAGCCAGATTTATTTATCTGATAAGGTAATGCTAATTTTAATA240.12381984212970129No Hit
CGTGGCGGTTGTTTTTGTTTTTGTTTTTGTTTTTGTTTTCCAACAATAGC240.12381984212970129No Hit
CGTGGCGGGGCTAGGCTGAGCTTATCGGAAGCCTTACCCACAAGCTGGAG240.12381984212970129No Hit
CGTGGCGGGCAGAAAGCTGAGCCCCAGTGATGATTGGTAGCTTTCATTCT240.12381984212970129No Hit
CGTGGCGGGCCACAAGCTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGA230.11866068204096374No Hit
CGTGGCGGGATCCTATACTGTCCCCCAGCCAGGGTCTATAAACCTGACAC230.11866068204096374No Hit
CGTGGCAGAGTGCAGTGCTCGTGGCGGGGGGCGCGGGGGGGGGCGCGAGT230.11866068204096374No Hit
CGTGGCGGACCCTAAATACGCCGATCTCCCCGGCATTGCCAGGAATGAGC230.11866068204096374No Hit
CGTGGCGGGAACAAAGGCTGAAGAGAGGCATGGAAATATTGAAGAACGTA230.11866068204096374No Hit
CGTGGCTGGAGAATTGGTATTGAATTATAGCATCAGCAGAACAGAAAATG230.11866068204096374No Hit
CGTGGCGGGATCCCAGACGTCCTTAAGTGCTAAATCTAAGGAACGTTGCC220.11350152195222618No Hit
CGTGGCGGGATACAACAAAGATAAGTGAGCCTGAGAGGAGGGCCCTGAGG220.11350152195222618No Hit
CGTGGCGGTGCACAGGGATCGTAGCCTGAAATACAGAGAAAGACAGATCT220.11350152195222618No Hit
CGTGGCGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGGAGGGCAGCGG210.10834236186348861No Hit
CGTGGCGGGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC210.10834236186348861No Hit
CGTGGCTCTTTGTAAATATAAGTGCAGCAATCTATGGCTTTTTAAGTTTT210.10834236186348861No Hit
CGTGGCGGCGGCAGTTCAGCATCTTCCCACTCTGCCTGTCAGGTTAGAAG210.10834236186348861No Hit
CGTGGCAGCTTCTTGACTGGCGTCACCCACTGGGGACAGCCTGGTCTAGG200.10318320177475106No Hit
CGTGGCGGGACAGAAGCACTGCAGGAAGCTTGACCATGGAGGCTGACTTC200.10318320177475106No Hit
CGTGGCGGGATCTCCTCCAGGAACACTGGCCAGCGAGCTGTGCTGAAGTT200.10318320177475106No Hit
CGTGGCGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCCCCC200.10318320177475106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAACT302.4006986E-644.00000414
GATGCCA302.4006986E-644.0000049
CGTGGCT650.044.0000041
CGGGCAG650.044.0000046
TGCCACA302.4006986E-644.00000411
ATGCCAC302.4006986E-644.00000410
CCACAAC302.4006986E-644.00000413
GTGGCCT302.4006986E-644.0000042
GTGGCAG600.044.0000042
GCGAGCT302.4006986E-644.0000045
TTATGGT207.665934E-444.08
CTCTGGT254.2822197E-544.036
TCCTCTA502.3646862E-1144.018
ACAGTAT207.665934E-444.016
GCGGGCT700.044.05
GCGGGCA700.044.05
GCGGGAT1550.044.05
AAACCTG254.2822197E-544.016
GCGGGAC1350.044.05
CATGATT407.608833E-944.032
Produced by FastQC (version 0.11.5)