FastQCFastQC Report
Fri 17 Jun 2016
SRR1527680_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527680_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134500
Sequences flagged as poor quality0
Sequence length52
%GC61

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCTGGGCTATCTGTCCTCTAGTCCCACCACTCCCTGGCCAGGTCATCC7800.5799256505576209No Hit
CGTGGCGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGG7390.5494423791821561No Hit
CGTGGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT5910.43940520446096654No Hit
CGTGGCCGGGACAAGCTATCGGACTGTGTGCTGCCATCGGTAATGAGTCTCA4580.3405204460966543No Hit
CGTGGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC4530.33680297397769515No Hit
CGTGGCGGGGTATAGCCCTGGCTGGAACTCACTTTGTAGGCCAGGCTGGCCT4280.3182156133828996No Hit
CGTGGCGGGAGAAAAGAAAATGAAGTTTTAGGACTTTCATGGTAACAAACCT4110.30557620817843867No Hit
CGTGGCGGGGTCTGCGCCGAGAAACCGAAGTGTAGGTGACGGTTCCGAGACA4100.3048327137546469No Hit
CGTGGCGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG4050.30111524163568776No Hit
CGTGGCGGGATCTCCATTAAAACTTGCCTTAAGCTTCCAGATTGCTTTGCCG4040.3003717472118959No Hit
CGTGGCTCTGTACTCTCCACTATACTCCTGCTGCTACCTCCACCGCCACTGG3840.28550185873605943No Hit
CGTGGCGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT3770.28029739776951673No Hit
CGTGGCGGGATGATCAGCAGTTCCGTTTACAGCTCACTCCATGTTCACACTT3360.24981412639405207No Hit
CGTGGCGGCATTTCTATCTGTACTGGGCTCAGAAGGCACAGTGGCCATATTG3280.2438661710037175No Hit
CGTGGCGGGGAACCCGGAAGTACTTCTCTGCAGAGGCCTGGGCGACTCTTTT3170.23568773234200743No Hit
CGTGGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT3130.23271375464684013No Hit
CGTGGCGGGGCTTTCGGAGCTGTGCGGCATTCTGAGCAGGAATGGCAGTGTG2970.220817843866171No Hit
CGTGGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT2830.2104089219330855No Hit
CGTGGCCTGATCCCACCCTGGGCACAGCCCATTGAGCCATCCTGCCACGCCA2770.20594795539033459No Hit
CGTGGCGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGC2730.20297397769516728No Hit
CGTGGCGGGACCTCGCTCCCAGACACCACAAGATACCAACAGAGCTGAAATA2710.20148698884758365No Hit
CGTGGCGGGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCAGT2680.1992565055762082No Hit
CGTGGCGGGCTGTGCAGTCTCAGTCACCCTCCACAAACTGGTCACCTGCAGT2580.19182156133828995No Hit
CGTGGCGGGGCTCCAGCTCCGCGTCCCGAAGGTGGGTCCCACGCGGGGTCAA2500.18587360594795538No Hit
CGTGGCGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG2390.17769516728624535No Hit
CGTGGCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG2360.1754646840148699No Hit
CGTGGCGGTTGTTTTTGTTTTTGTTTTTGTTTTTGTTTTCCAACAATAGCTT2330.1732342007434944No Hit
CGTGGCGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAA2330.1732342007434944No Hit
CGTGGCGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT2330.1732342007434944No Hit
CGTGGCGGGACTATAGCTCAGATTGCTGGGCATTAAGTGTGCTAGTGGCTCA2290.17026022304832714No Hit
CGTGGCGAGCCCCCAACTACCGCCTGCATGTGGACGGTGGTCCTCACGGTCG2280.16951672862453532No Hit
CGTGGCGGGAAATTGGAATGCCCTGAGTTCAGCTCAGAGCCAGCATCTGTTG2250.16728624535315986No Hit
CGTGGCGAGCAATGGCTGCTGCCAAGGCCCCCACAAAGGCAGCACCTAAACA2190.16282527881040892No Hit
CGTGGCGGGGACTTAGCAAATATCACCTGCGAGATTGCGATTAAGCAGAAGC2180.1620817843866171No Hit
CGTGGCGGGAAGGCACAAGAGGACCTCTAAAGCAACAGCCAAATGCACCACT2170.1613382899628253No Hit
CGTGGCGGGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2140.1591078066914498No Hit
CGTGGCGGGATCTTGGACCTCTCCAAGTACATCGATAAGACCATTCGGGTGA2100.15613382899628253No Hit
CGTGGCGGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGC2080.1546468401486989No Hit
CGTGGCGGGCTCCACCATATCACACATTCGAGGTAACCAACCTATGTAAAAG2070.15390334572490705No Hit
CGTGGCGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGAT2030.15092936802973977No Hit
CGTGGCGGGGCTTCCACAGGAGGCCTACACGCCGCCGTCTGTGCCGCCGCCA2020.15018587360594796No Hit
CGTGGCGGGACCGCCGTACGCAATGCCCTTCAGCCTAGAGCGCGGAGGAGGA1960.14572490706319702No Hit
CGTGGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG1950.14498141263940523No Hit
CGTGGCGGGGTTCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG1930.14349442379182156No Hit
CGTGGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG1910.14200743494423793No Hit
CGTGGCGGGACTCTGCAGACTCAGTGCCTTACTCAGACTCCTCTCTCGCTCT1900.1412639405204461No Hit
CGTGGCGGGCTGGAGTCTCTCGGGACACGACCAGCTGATTGTAGACACCGTG1870.13903345724907062No Hit
CGTGGCGGGGGTCTGGGGAAATGCGGGTGCCCGGGCAAGATGGCGGCGCACC1860.1382899628252788No Hit
CGTGGCGGGCTCAAAGTCTTCCTTAACCCTATGGAGCAAGAAGCTGCCACTG1840.13680297397769517No Hit
CGTGGCGGGACATCTTTCTCAAATTAAGAAATTATGTACCAAGTCTATGCAT1790.13308550185873605No Hit
CGTGGCGGGACACGCAGCAGGCGGTGAGGACGTGCGCGCCCCCACTTCTTCC1780.13234200743494426No Hit
CGTGGCGGGGCTTAAAGCTTCCTGGTGCCTGTCTGCCAGGTCATGGAGGATT1760.1308550185873606No Hit
CGTGGCGGCGGACCGAGCCGGTGCCGGGGGAAGAGCCTGCTGAGGGATGCCC1740.12936802973977696No Hit
CGTGGCGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT1740.12936802973977696No Hit
CGTGGCGGGGGAGAAGCCCCGATGGCTGGGGAAATATCAGATCTGTCAGCGA1710.1271375464684015No Hit
CGTGGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC1660.12342007434944238No Hit
CGTGGCGGGGTTCAAGGGCAGCCCGTGCTAGATGAGGCCCTGCCTCCTAATA1650.12267657992565055No Hit
CGTGGCGGGCTCTTCGGTTCTCATCGCTGTGCGGACGCCAGAGCCGAGCCCG1600.11895910780669146No Hit
CGTGGCGGGGGGGCTACAGTGCTGAGTGAGTTCTAAGTAAAACACATTCCAC1600.11895910780669146No Hit
CGTGGCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA1600.11895910780669146No Hit
CGTGGCGAGAGAGGAGAATGAAGAGGCCAAGAGTGGAGAAGACTAGAAGGCT1580.1174721189591078No Hit
CGTGGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGGCTTTCTGTGAGG1570.11672862453531599No Hit
CGTGGCGGGGCTCTCCAGCTCCGGCGCCGTAGCCATCATGAATGACACAGTA1560.11598513011152416No Hit
CGTGGCGGGCAACTGTAGTTATTTGTAATCTAATAATTTTCATTAATACCAA1550.11524163568773234No Hit
CGTGGCGGGACCAAGAAAGGTAAAGAAAGAACTAAGACAAAAAAAAAAAAAA1540.11449814126394053No Hit
CGTGGCGGGATGGCCATGAAGGGAAAGAACTGTGTGGCCATCGCTGCAGACA1520.11301115241635687No Hit
CGTGGCGGGATCACAGTGCAGCAAGGTTCTTCCTAGGATAGTGTGTGAGGAC1510.11226765799256506No Hit
CGTGGCGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTAT1510.11226765799256506No Hit
CGTGGCGGGGTCCTGCTCTCCTGACACATCTTTGAGCTTTTCCCACCGTGTT1490.11078066914498141No Hit
CGTGGCGGGGGCATGGCAGCGAGCACGGACATAGCTGGGCTGGAGGAGAGCT1490.11078066914498141No Hit
CGTGGCGGGGGTGAGCTGTCAGGATCCTAGCAGTGGGAGGCAGGGATCGGAC1490.11078066914498141No Hit
CGTGGCGGGCGGTGATCTGGAAACGCGAAAGATGGCGACTGTTCGGCGACGT1480.11003717472118958No Hit
CGTGGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG1470.10929368029739778No Hit
CGTGGCGGGGCTTACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCC1450.10780669144981411No Hit
CGTGGCGGGGCCTTTCGCTGATGCAAGAGCCTAGTGCGGTGCTGTGAGAAGT1450.10780669144981411No Hit
CGTGGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGGCTTTCTGTGAG1450.10780669144981411No Hit
CGTGGCGGGGCCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTC1450.10780669144981411No Hit
CGTGGCGGTGGGAGGACTGAAGGAAGATGGGTCAAAGCCAGAGTGGTGGCCA1410.10483271375464683No Hit
CGTGGCGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG1410.10483271375464683No Hit
CGTGGCGGGATCCTATACTGTCCCCGAGCCAGGGTCTATAAACCTGACACTT1410.10483271375464683No Hit
CGTGGCGGGGCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAAAGGA1410.10483271375464683No Hit
CGTGGCGGGATGCAGTCTTGAGTGCCCTAAGTTGTCAGCCCAACAGAGTTTG1400.10408921933085502No Hit
CGTGGCCCAGTCAGCTGACCAGCTAGAATCACATATTTATTTTCCATTTGTA1400.10408921933085502No Hit
CGTGGCGGGACGCTCAGGAGCAGCCAAGCGGTAGAAGCCGGGGGGCATGGCA1400.10408921933085502No Hit
CGTGGCGGAGGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGC1390.1033457249070632No Hit
CGTGGCGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGTGG1390.1033457249070632No Hit
CGTGGCGGGGCTGTACATAGAAGCCGAGGGCTTGTCTGGATTCAAGAATGAT1390.1033457249070632No Hit
CGTGGCGGAGTGACTCTTTGGCCTCTCTTTGGCCATCCTGGTGAACAGCAAG1380.10260223048327137No Hit
CGTGGCGGGGTCTGAGGTCACTTGGAAATGGTGGCCCCTAACCTGTGCCTGA1380.10260223048327137No Hit
CGTGGCGGGCCACAAGCTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGT1370.10185873605947957No Hit
CGTGGCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.10185873605947957No Hit
CGTGGCGGGGTTTGCCAGCTTGCGGCACCGGGAATGATGGACAGCCACGTGG1370.10185873605947957No Hit
CGTGGCGGGGCCTGGAGGAGCTCACCAACCGCCTGGCCTCGCTGCAGCAGGA1360.10111524163568773No Hit
CGTGGCGGTAATTGGACTTTGCATGTTACAAAAAGAGGAGGTCACAATGTGT1350.1003717472118959No Hit
CGTGGCGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTG1350.1003717472118959No Hit
CGTGGCGGGAAGGAAGTTCAGTTAAAGATTAACAGCTAAAAATCAAGGCTGA1350.1003717472118959No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAA206.291498E-446.00000423
TATTTCT206.291498E-446.00000421
AACGTTC206.291498E-446.00000422
CTGCTCA206.291498E-446.00000419
CAGAATG206.291498E-446.00000430
AACGTGA206.291498E-446.00000415
TGTCAGA405.5479177E-946.00000433
CTAGAAT206.291498E-446.00000423
CGTGGAA206.291498E-446.00000444
TTAGGAC405.5479177E-946.00000428
AACAAAC405.5479177E-946.00000444
CTGTCAC206.291498E-446.00000418
GTACCGG206.291498E-446.00000413
CGAACGT206.291498E-446.00000420
GCGACTC206.291498E-446.00000442
ATGATTA206.291498E-446.00000433
TCCGCTA206.291498E-446.00000411
ACGCCGT206.291498E-446.00000424
GCGACGT206.291498E-446.00000446
TACTTTG206.291498E-446.00000444
Produced by FastQC (version 0.11.5)