FastQCFastQC Report
Fri 17 Jun 2016
SRR1527679_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527679_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136983
Sequences flagged as poor quality0
Sequence length52
%GC61

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCTGGGCTATCTGTCCTCTAGTCCCACCACTCCCTGGCCAGGTCATCC7680.5606535117496332No Hit
CGTGGCGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGG7060.515392420957345No Hit
CGTGGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT6140.448230802362337No Hit
CGTGGCCGGGACAAGCTATCGGACTGTGTGCTGCCATCGGTAATGAGTCTCA4580.3343480577881927No Hit
CGTGGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC4390.3204777235131367No Hit
CGTGGCGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG3910.2854368790287846No Hit
CGTGGCGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT3880.2832468262485126No Hit
CGTGGCGGGGTCTGCGCCGAGAAACCGAAGTGTAGGTGACGGTTCCGAGACA3860.2817867910616646No Hit
CGTGGCGGGATCTCCATTAAAACTTGCCTTAAGCTTCCAGATTGCTTTGCCG3630.26499638641291257No Hit
CGTGGCGGGAGAAAAGAAAATGAAGTTTTAGGACTTTCATGGTAACAAACCT3620.26426636881948856No Hit
CGTGGCGGGGAACCCGGAAGTACTTCTCTGCAGAGGCCTGGGCGACTCTTTT3500.2555061576984005No Hit
CGTGGCGGGGTATAGCCCTGGCTGGAACTCACTTTGTAGGCCAGGCTGGCCT3450.2518560697312805No Hit
CGTGGCGGCATTTCTATCTGTACTGGGCTCAGAAGGCACAGTGGCCATATTG3300.2409058058299205No Hit
CGTGGCGGGATGATCAGCAGTTCCGTTTACAGCTCACTCCATGTTCACACTT3260.2379857354562245No Hit
CGTGGCTCTGTACTCTCCACTATACTCCTGCTGCTACCTCCACCGCCACTGG3150.2299555419285605No Hit
CGTGGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT2940.21462517246665647No Hit
CGTGGCCTGATCCCACCCTGGGCACAGCCCATTGAGCCATCCTGCCACGCCA2760.20148485578502442No Hit
CGTGGCGGGGACTTAGCAAATATCACCTGCGAGATTGCGATTAAGCAGAAGC2680.1956447150376324No Hit
CGTGGCGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGC2680.1956447150376324No Hit
CGTGGCGGGACCTCGCTCCCAGACACCACAAGATACCAACAGAGCTGAAATA2600.1898045742902404No Hit
CGTGGCGGGACTATAGCTCAGATTGCTGGGCATTAAGTGTGCTAGTGGCTCA2550.1861544863231204No Hit
CGTGGCGGGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCAGT2500.18250439835600038No Hit
CGTGGCGGGGCTTTCGGAGCTGTGCGGCATTCTGAGCAGGAATGGCAGTGTG2490.18177438076257638No Hit
CGTGGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT2480.1810443631691524No Hit
CGTGGCGGGAAATTGGAATGCCCTGAGTTCAGCTCAGAGCCAGCATCTGTTG2430.17739427520203238No Hit
CGTGGCGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAA2400.17520422242176037No Hit
CGTGGCGGGGAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGC2260.16498397611382434No Hit
CGTGGCGAGCCCCCAACTACCGCCTGCATGTGGACGGTGGTCCTCACGGTCG2230.16279392333355233No Hit
CGTGGCGGGCTGTGCAGTCTCAGTCACCCTCCACAAACTGGTCACCTGCAGT2210.16133388814670435No Hit
CGTGGCGGTTGTTTTTGTTTTTGTTTTTGTTTTTGTTTTCCAACAATAGCTT2180.1591438353664323No Hit
CGTGGCGAGCAATGGCTGCTGCCAAGGCCCCCACAAAGGCAGCACCTAAACA2160.15768380017958433No Hit
CGTGGCGGGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.15476372980588832No Hit
CGTGGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG2080.15184365943219233No Hit
CGTGGCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA2080.15184365943219233No Hit
CGTGGCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG2070.15111364183876833No Hit
CGTGGCGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG2060.15038362424534432No Hit
CGTGGCGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT2040.14892358905849631No Hit
CGTGGCGGGCTCAAAGTCTTCCTTAACCCTATGGAGCAAGAAGCTGCCACTG1970.1438134659045283No Hit
CGTGGCGGGCTCCACCATATCACACATTCGAGGTAACCAACCTATGTAAAAG1960.14308344831110428No Hit
CGTGGCGGGACTCTGCAGACTCAGTGCCTTACTCAGACTCCTCTCTCGCTCT1900.1387033427505603No Hit
CGTGGCGGGGTTCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG1840.13432323719001626No Hit
CGTGGCGGGACATCTTTCTCAAATTAAGAAATTATGTACCAAGTCTATGCAT1810.13213318440974428No Hit
CGTGGCGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGAT1810.13213318440974428No Hit
CGTGGCGGGCTGGAGTCTCTCGGGACACGACCAGCTGATTGTAGACACCGTG1760.12848309644262426No Hit
CGTGGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC1750.12775307884920026No Hit
CGTGGCGGGGCTTCCACAGGAGGCCTACACGCCGCCGTCTGTGCCGCCGCCA1740.12702306125577625No Hit
CGTGGCGGGACCGCCGTACGCAATGCCCTTCAGCCTAGAGCGCGGAGGAGGA1730.12629304366235228No Hit
CGTGGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG1690.12337297328865626No Hit
CGTGGCGGGAAGGCACAAGAGGACCTCTAAAGCAACAGCCAAATGCACCACT1680.12264295569523226No Hit
CGTGGCGGGGGAGAAGCCCCGATGGCTGGGGAAATATCAGATCTGTCAGCGA1670.12191293810180825No Hit
CGTGGCGGGCTCTTCGGTTCTCATCGCTGTGCGGACGCCAGAGCCGAGCCCG1650.12045290291496025No Hit
CGTGGCGGGGTTCAAGGGCAGCCCGTGCTAGATGAGGCCCTGCCTCCTAATA1620.11826285013468825No Hit
CGTGGCGGGGGGGCTACAGTGCTGAGTGAGTTCTAAGTAAAACACATTCCAC1610.11753283254126423No Hit
CGTGGCGGGGCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAAAGGA1600.11680281494784026No Hit
CGTGGCGGCGGACCGAGCCGGTGCCGGGGGAAGAGCCTGCTGAGGGATGCCC1590.11607279735441625No Hit
CGTGGCGGGGGCATGGCAGCGAGCACGGACATAGCTGGGCTGGAGGAGAGCT1590.11607279735441625No Hit
CGTGGCGGGGCTTAAAGCTTCCTGGTGCCTGTCTGCCAGGTCATGGAGGATT1570.11461276216756823No Hit
CGTGGCGGGGCTCCAGCTCCGCGTCCCGAAGGTGGGTCCCACGCGGGGTCAA1550.11315272698072024No Hit
CGTGGCGGGACCAAGAAAGGTAAAGAAAGAACTAAGACAAAAAAAAAAAAAA1540.11242270938729623No Hit
CGTGGCGGGATCTTGGACCTCTCCAAGTACATCGATAAGACCATTCGGGGGA1490.10877262142017623No Hit
CGTGGCGGGATCCTATACTGTCCCCGAGCCAGGGTCTATAAACCTGACACTT1470.10731258623332823No Hit
CGTGGCGGGGGTGAGCTGTCAGGATCCTAGCAGTGGGAGGCAGGGATCGGAC1470.10731258623332823No Hit
CGTGGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGGCTTTCTGTGAGG1460.10658256863990423No Hit
CGTGGCGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG1440.10512253345305621No Hit
CGTGGCGGGGCTTACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCC1440.10512253345305621No Hit
CGTGGCGGGATGGCCATGAAGGGAAAGAACTGTGTGGCCATCGCTGCAGACA1440.10512253345305621No Hit
CGTGGCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.10512253345305621No Hit
CGTGGCGGGCCACAAGCTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGT1430.1043925158596322No Hit
CGTGGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG1430.1043925158596322No Hit
CGTGGCGGTGGGAGGACTGAAGGAAGATGGGTCAAAGCCAGAGTGGTGGCCA1410.10293248067278421No Hit
CGTGGCGGGGTCCTGCTCTCCTGACACATCTTTGAGCTTTTCCCACCGTGTT1410.10293248067278421No Hit
CGTGGCGAGCTTCTTAAACCTGAAGTGGAGTACTACTCTGGTTGCGCTTGTT1400.1022024630793602No Hit
CGTGGCGGGGCTCACAGCCATTGCAGTACATTGAGCTCCATAGAGACAGCGC1400.1022024630793602No Hit
CGTGGCGGAGTGACTCTTTGGCCTCTCTTTGGCCATCCTGGTGAACAGCAAG1400.1022024630793602No Hit
CGTGGCGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTCA1390.1014724454859362No Hit
CGTGGCGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAATT1390.1014724454859362No Hit
CGTGGCGGGATTTGAGACTAACTCAATTTTATCATCTTATTACTTAGGCACA1390.1014724454859362No Hit
CGTGGCGGTGCCAGTCTAGAGCTCCTGGAAGGTAAAGTCCTTCCTGGGGTGG1380.10074242789251221No Hit
CGTGGCGGGGGTCTGGGGAAATGCGGGTGCCCGGGCAAGATGGCGGCGCACC1380.10074242789251221No Hit
CGTGGCGGGGCCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTC1380.10074242789251221No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAG206.2919024E-446.00000429
TCACGGT206.2919024E-446.00000444
TGATCCG206.2919024E-446.00000419
TATTTTC206.2919024E-446.00000438
TATCATT206.2919024E-446.00000436
CTTAACC405.5497367E-946.00000422
CTTAAAG351.010776E-746.00000411
TTGATAC206.2919024E-446.00000439
CAGGTCC206.2919024E-446.00000444
AGGTATA206.2919024E-446.00000433
GAGTGTA206.2919024E-446.00000438
AAGTACA351.010776E-746.00000425
TAGAGAC206.2919024E-446.00000441
GGTACTG206.2919024E-446.0000049
ACTATAG351.010776E-746.00000410
ACTATAC351.010776E-746.00000419
TGATACA206.2919024E-446.00000440
GTACCAA351.010776E-746.00000436
GTTCTTC351.010776E-746.00000426
ACCGGAT206.2919024E-446.00000433
Produced by FastQC (version 0.11.5)