FastQCFastQC Report
Fri 17 Jun 2016
SRR1527675_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527675_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30821
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16765.437850816002077No Hit
ATGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15515.032283183543687No Hit
ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6502.1089516887836215No Hit
ATGGCGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6151.9953927516952727No Hit
ATGGCGAGGGGCGCATTGCTGCACCTCCAGGGCTGGAGAGCTTCCTTGAT5141.6676941046688945No Hit
ATGGCGACCATCCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCG2950.9571396126017975No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2950.9571396126017975No Hit
ATGGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT2880.9344278251841276No Hit
ATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTG2780.9019824145874566No Hit
ATGGCGAGGCGCTTGGTGGTAGGGGCACTCACCTTTAATCCAAGGACTGG2670.8662924629311184No Hit
ATGGCGGTGACTTTGTAGAGATTGAAGTGGAGGAGTTAAGACACATTGAC2490.8078907238571104No Hit
ATGGCGCCACCAGCAAGAGCAGAAGCAATACCGCCAGCAATAGCACCAAA2370.7689562311411051No Hit
ATGGCGAGAAATAAAAGTCTGAAACATGATTAAACTCCTAAGCAGAAAAC2040.6618863761720905No Hit
ATGGCGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2020.6553972940527562No Hit
ATGGCGAGGCACTCCTCCAGGTTCTTTCAGATTCTCCCTGTTACCTACCC1940.6294409655754194No Hit
ATGGCGAAAGGTCGCAAAGTAAGAGCTTCTCGAGCTGCGCAAGGATAGGT1710.5548165212030758No Hit
ATGGCGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCC1680.5450828980240745No Hit
ATGGCGAGGGGAGTCCCAGGGCACACAGCTCATCTCGATCCTTGTGGGTT1640.532104733785406No Hit
ATGGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.5093929463677362No Hit
ATGGCGAGGCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA1560.5061484053080691No Hit
ATGGCGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.4834366178903994No Hit
ATGGCGGAGAGAGACAGAGAGACAGAGAGACAGAGAGACAGAGAGAGAAG1380.44774666623406123No Hit
ATGGCGGAGTGAACAAGACGCTAACCCGTAGACAACCGTCAGCATGCTCG1360.441257584114727No Hit
ATGGCGGGGCATGCACAGCACACACATCCATGAAATGAAAATCACTAAAG1350.4380130430550599No Hit
ATGGCGAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG1240.4023230913987217No Hit
ATGGCGAGGGGCCGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCT1090.353654975503715No Hit
ATGGCGGGGCACACATACACACACATACATATGCACACAAACTTAACAAA1060.34392135232471366No Hit
ATGGCGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020.3309431880860452No Hit
ATGGCGAGGATGTGAAAGCTGAAGTACACAAATCCTTGGACAGTTACGCA1010.3276986470263781No Hit
ATGGCGGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA960.3114759417280426No Hit
ATGGCGAGGGAGCTTCGGGCTGCCCAAGGACTTTGTAGACCTTTCCACCG950.30823140066837545No Hit
ATGGCGGGGCACATCACCTGCTCTACCAACTGGTTAGAAACAATAGTCTC950.30823140066837545No Hit
ATGGCGGGGACTCCAGTGTCTTGTACTGACTGCTGCCAATCTGTGAAAAT950.30823140066837545No Hit
ATGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTG860.2790305311313715No Hit
ATGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG840.27254144901203725No Hit
ATGGCGGAGATTTTGACTGATTGTGCATCCTGGAGTAGAATAATCCCTTT820.266052366892703No Hit
ATGGCGGAGAGAAAACTGGGTAACTGCTCAGTGCATGAAGCTGGATGCTC780.2530742026540346No Hit
ATGGCGAGGGGGGAGCCACGGATGATTTCTGGGCTGTGGGGCTTTGAAGA770.24982966159436745No Hit
ATGGCGGGGCATTCGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAGG770.24982966159436745No Hit
ATGGCGAGGCGACATCGCCAAGGAGCTCTGCCTGCCGCCTGTGAAACTGC770.24982966159436745No Hit
ATGGCGGGGCACAGCCTTGGAAACACTGCACCAGAACAGCATCACTCAAG760.24658512053470036No Hit
ATGGCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA750.24334057947503324Illumina Single End Adapter 2 (97% over 34bp)
ATGGCGGAGAGCCGGCCGCTCCCTCCCTCTGGCAGCTAGCAGGGCAATTG750.24334057947503324No Hit
ATGGCGGAGGGTGCAACGTCCGAGCTGCTAAGTGCGTCAGTTGTGGAGAG730.23685149735569902No Hit
ATGGCGAGGCAGTATAATCACGAAAAGAATGCAGCACTGCAGCATATAAT730.23685149735569902No Hit
ATGGCGATGGCTCCACTGAAGAGACAGATGAGTCTGAGACTTGAATCTGA720.2336069562960319No Hit
ATGGCGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.22711787417669774No Hit
ATGGCGGTGGCATGCGGGTGGGACCCCAGTACCAAGCGGCGGTGCCCGAC680.2206287920573635No Hit
ATGGCGGGGCTAAGTTTTAAGCACATGCCTTTTGTATTATGCAGTATATA680.2206287920573635No Hit
ATGGCGAGGAATTGGGAAAATATTCTTGTGTCTTTGGAGAAAAGGAACTA680.2206287920573635No Hit
ATGGCGGGGATTAGCTCTCGGGAGAACATCTTGGTTTCCATGGGAGCTGT650.21089516887836218No Hit
ATGGCGGGGTCTAGCAGCTAAGATGATGTCCTTGTCAGCTATTGCTAATG650.21089516887836218No Hit
ATGGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.20765062781869506No Hit
ATGGCGAGGGTTAGTTGAACTTTGTAAAAATATATATATATACTTGTTTT630.20440608675902794No Hit
ATGGCGAGCCGCTGTCTTCCCTTCCTGCCATGGGCCCACCTTCTGACCCT620.20116154569936084No Hit
ATGGCGGGGCAAAGCTAGTTTAAGAAAACCTTGCTGTGTACACATGTCTA600.1946724635800266No Hit
ATGGCGGAGCGGGAAGACCGGGCCTTACCCTGCCTGGGCGCAGGTAGGCA600.1946724635800266No Hit
ATGGCGGGGCTAACATTACTGCAGGACACTTATTAATACACCTAATCGGA580.18818338146069238No Hit
ATGGCGAGGATCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.18493884040102526No Hit
ATGGCGGGGCTGTCCTCTTACCCTCCAAGAGGATCAGTGATTACTGGATC540.17520521722202395No Hit
ATGGCGGGGACATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAAT540.17520521722202395No Hit
ATGGCGGGGCGAAGAAGATTCATCGCAGAGTCTTGAAGAAGAATCCACTG540.17520521722202395No Hit
ATGGCGAGGGACAGTCACTTGCCAGTAATCAGATATTTTAGGAAGTGGCT540.17520521722202395No Hit
ATGGCGGGGGGGGGGGATTTTTCCTTTATTATGTGCAACTGTAGTTATTT540.17520521722202395No Hit
ATGGCGGGGGGCTCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.17196067616235683No Hit
ATGGCGGAGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT520.1687161351026897No Hit
ATGGCGAGGGTTTTGGTATTGTCACAATAACTGTATTTTATTCCTGAGAT520.1687161351026897No Hit
ATGGCGAGGAGAGATGGTTCGGCACGCCCTGGATGCCCACCAGAGGTCCA510.1654715940430226No Hit
ATGGCGTGTGGGATGACTATGTGCCCAAGCTGTACGAGCAGCTGTCAGGG510.1654715940430226No Hit
ATGGCGGAGAGAGTGCAGTGCTATGGGGGGGAAAAAAAAAAAAAAAAAAA500.1622270529833555No Hit
ATGGCGGGGCGACGACGATGATGATGACGACGATGATGAAGATGACGACG500.1622270529833555No Hit
ATGGCGAGGAGTTATTAAAGTTTTCAGACCCTCCTGAATTCAGATGTAAT500.1622270529833555No Hit
ATGGCGGAATAACTCCAGCCGCGGCCCTACTGACTGGTCCTCCGTGTCCC490.1589825119236884No Hit
ATGGCGGTGGGTGGTATCAACGCAGAGAGCAGGCTATGGCCGGCCTCTGT480.1557379708640213No Hit
ATGGCGGAAGAGTTACAATAAAGTTTACAAGCGAGAAAAAAAAAAAAAAA480.1557379708640213No Hit
ATGGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.1557379708640213No Hit
ATGGCGAGGAGTGTGCTCTCGGGCCGCTTTTTTAAAAGGCCATGTTTATA470.15249342980435418No Hit
ATGGCGGGGAGCCTTTCCAAAGAGCCTAGCAAACCCAAGACAGGAAAGGC460.14924888874468706No Hit
ATGGCGGGGCGAAACACAGTAGAGCTTCAGGCAGAGAGTGGCAAGAGGAA460.14924888874468706No Hit
ATGGCGGGGCACAACTAGATACATCAACATGATTTATCACAATTATCTCA450.14600434768501996No Hit
ATGGCGGAGACTGTGGCTCCTTCAAAAAGGATCACGGAGACTGTTTCCCT450.14600434768501996No Hit
ATGGCGGAGCAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.14600434768501996No Hit
ATGGCGGGACATCCCTTTCAGAGAGCCAGGCCACCACCAGGGCCCCTGGC450.14600434768501996No Hit
ATGGCGGGGTTCAGGAAGAGGTGGCAGTAACCTCCAAAGCCAAGTTACAT450.14600434768501996No Hit
ATGGCGGGGTCCAGCTATACTATGAGCCTTAGGCTTTATTTTCTTATTTA450.14600434768501996No Hit
ATGGCGGGGGATGAGGGCCCACCAGCCTGACAGAAGAGCACCCAGTGTGT440.14275980662535284No Hit
ATGGCGAGGAGGAGGATGAAATTTTGAACAGATCGCCAAGAAACAGAAAG440.14275980662535284No Hit
ATGGCGAGGAAGGACACTGGGAGGTGTGTGAACGCCATGGTACCGGCGGC430.13951526556568575No Hit
ATGGCGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.13951526556568575No Hit
ATGGCGGGGATCAGGTCTAGTAATATGATTTCACTATAATTCAATTACAC420.13627072450601863No Hit
ATGGCGGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.13627072450601863No Hit
ATGGCGGGGCTGCATGCACGGGAAAGCAAGAGTGTGCCTGCCTGAGTGAT420.13627072450601863No Hit
ATGGCGGGCTCTAACAATAAACCAAGTTCATTGCAAAAAAAAAAAAAAAA410.1330261834463515No Hit
ATGGCGGGGTGCAGAGAAGAGAGGCACCTTCTGCAAAGACCTCTTCCACT400.1297816423866844No Hit
ATGGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA400.1297816423866844Illumina Single End Adapter 2 (97% over 34bp)
ATGGCGAGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.1297816423866844No Hit
ATGGCGGGGGCCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.12329256026735018No Hit
ATGGCGGGGTGCAGTACCCCATAGACTGGTGTTAAGTGTTGTCTACAGTG380.12329256026735018No Hit
ATGGCGGCGATTGCGTACCCGACGACCAAAATTAGGGTCAACGCTACCTG380.12329256026735018No Hit
ATGGCGAGGATTACACTATAATATAAAATCTGATATATACACGTTAGGAA380.12329256026735018No Hit
ATGGCGGGGCCACCAGGGGGCTCTGTCACAGCCACACCAGGCTGAGCACT370.12004801920768307No Hit
ATGGCGGAGTCAGAAGGGCGGGGAACGCGGCTAAGTGTTGGCGATTCGGA370.12004801920768307No Hit
ATGGCGGGGGTCTTCCTTCCTGCCTTGTCCCGCACCTTTCGCGACATGGG360.11680347814801595No Hit
ATGGCGGGATCTCAGGACAAAAGAATGAACAGTTGTATGTAAAATTGATA360.11680347814801595No Hit
ATGGCGGGGCGAAGGATGAGGATACCTTGGGCATCAGCTCCCCGGCTGCC350.11355893708834887No Hit
ATGGCGGAGAGATAATTATCTAAAGAAAACATACTATTAATCAGAATCTC350.11355893708834887No Hit
ATGGCGAGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.11031439602868175No Hit
ATGGCGAGGAGGGTCACATGGTAGGAAGACGGTCTTTTTTTCTGTGGAAG340.11031439602868175No Hit
ATGGCGGGGATCAGCTGCCCCTACAAGAGCACTTCAGACAACCAAGCCTC340.11031439602868175No Hit
ATGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTT340.11031439602868175No Hit
ATGGCGGAGGCCCTTTAAATTTTAAGTGACAGTACAGAAAAATCAAGGTG330.10706985496901464No Hit
ATGGCGGGGCGCAGGGCCAAGGGCATGTTCACCGCTGAAGACCTGCGCTA330.10706985496901464No Hit
ATGGCGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGC320.10382531390934753No Hit
ATGGCGGGGATGTGCGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.10382531390934753No Hit
ATGGCGGAGAAGAAAGGCTTATCTTTATATTTATACATGCCTACAAGGAA310.10058077284968042No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAATC207.73761E-444.00000428
ACAGCCT207.73761E-444.00000411
CGTTTAC207.73761E-444.00000440
ACAGCAT207.73761E-444.00000436
TTTGAAG207.73761E-444.00000443
GAGCGTC207.73761E-444.0000049
TCCTCGT207.73761E-444.00000443
TTGTTCG207.73761E-444.00000433
AATCCTT207.73761E-444.00000431
GGATGAT207.73761E-444.00000420
AAGTACA207.73761E-444.00000422
TGCACCA207.73761E-444.00000427
TGGCAGG207.73761E-444.00000444
TCCCAGG207.73761E-444.00000414
CGGAGGT207.73761E-444.0000045
CAGCATC207.73761E-444.00000437
CGGAGGA207.73761E-444.0000045
CTGAGCA207.73761E-444.00000426
CTATACA207.73761E-444.00000419
TAAACAT207.73761E-444.00000420
Produced by FastQC (version 0.11.5)