FastQCFastQC Report
Fri 17 Jun 2016
SRR1527674_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527674_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165603
Sequences flagged as poor quality0
Sequence length52
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19351.168457093168602No Hit
ATGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15110.9124230841228721No Hit
ATGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10250.6189501397921535No Hit
ATGGCGGGGATCCAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGG8680.5241450939898431No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8040.485498451115016No Hit
ATGGCGAGGGGCGCATTGCTGCACCTCCAGGGCTGGAGAGCTTCCTTGATGC7440.4492672234198656No Hit
ATGGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG5280.3188348037173239No Hit
ATGGCGGGGGGGAGGGCTGCCTGAGAAACACCGTGTACGGGGACGACAGCGG4500.2717342077136284No Hit
ATGGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4480.27052650012379No Hit
ATGGCGGGGCGACGACGATGATGATGACGACGATGATGAAGATGACGACGAC4180.2524108862762148No Hit
ATGGCGGGGCGCAGGGCCAAGGGCATGTTCACCGCTGAAGACCTGCGCTAGG3630.21919892755566023No Hit
ATGGCGAGGCGCTTGGTGGTAGGGGCACTCACCTTTAATCCAAGGACTGGAG3420.20651799786235758No Hit
ATGGCGGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTATC3290.1986678985284083No Hit
ATGGCGGGGCACATCACCTGCTCTACCAACTGGTTAGAAACAATAGTCTCAC3240.19564862955381243No Hit
ATGGCGGGGCATTCGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAGGAA3180.1920255067842974No Hit
ATGGCGGGGGTTTGCTGGCTATACAGACAACTTGGTGCGAGTATGGCAGGGA3170.19142165298937822No Hit
ATGGCGGGGGATAATCACAGGAAAAGATGCCTTCTAAAGACTTGGGCATAAA3120.18840238401478235No Hit
ATGGCGGGGGGGTGGGATTTTTCCTTTATTATGTGCAACTGTAGTTATTTGT3100.187194676424944No Hit
ATGGCGGGGAGAAACTGCCAGCCGAGATCACAAGCATTGGGGCTGAAGCAGG2990.1805522846808331No Hit
ATGGCGGGGACTCCCTTGTACATTTCTGCCACTTCAGTAAAAAGATGCTCAT2900.1751176005265605No Hit
ATGGCGGTGACTTTGTAGAGATTGAAGTGGAGGAGTTAAGACACATTGACTG2830.1708906239621263No Hit
ATGGCGGGGGGGGGGGATTTTTCCTTTATTATGTGCAACTGTAGTTATTTGT2830.1708906239621263No Hit
ATGGCGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG2800.16907906257736877No Hit
ATGGCGGGGGTCTTGTGTAACTTGATGTACATTTTAAATGGCCAGCCCAGCA2780.16787135498753042No Hit
ATGGCGGGGTGCTGGGTCTCACAGTATCCCCAAGGGGTGCACACTGGGTGAG2760.16666364739769207No Hit
ATGGCGGGGAACAAAACAAAACAAAAACCAAAAGGATGCATTATCATCTTGG2690.16243667083325786No Hit
ATGGCGGGGGCTTGCAGCTGCCCCACAGTGCTTGACCTCTGAGTCATTGGTT2570.15519042529422775No Hit
ATGGCGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGG2480.14975574113995518No Hit
ATGGCGGGGTGCCTAAAGATGTAAACAATAGAGCACGCTCTGCGCTTTGGCG2380.14371720319076345No Hit
ATGGCGGGGGTTCAGGAAGAATATACTCAGTGTCTGTCTCCACGCTGCTTTA2340.14130178801108675No Hit
ATGGCGAGGCACTCCTCCAGGTTCTTTCAGATTCTCCCTGTTACCTACCCAA2310.13949022662632923No Hit
ATGGCGGGGAACAGCGACGGCACCATCGACTTCCGGGAGTTCATCATTGCTC2270.13707481144665254No Hit
ATGGCGAGGGGAGTCCCAGGGCACACAGCTCATCTCGATCCTTGTGGGTTGG2270.13707481144665254No Hit
ATGGCGGGGTTCATGGCTGCCCTCCACCATATCACACATTCGAGGAACCAAC2200.1328478348822183No Hit
ATGGCGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2170.13103627349746078No Hit
ATGGCGGGGGAGGGCTGGCGAGATGGCTCAGAGAGTAAGAACACTGACTGCT2150.12982856590762246No Hit
ATGGCGGAGTGAACAAGACGCTAACCCGTAGACAACCGTCAGCATGCTCGCT2110.12741315072794576No Hit
ATGGCGGGGAGCCTTTCCAAAGAGCCTAGCAAACCCAAGACAGGAAAGGCTG2110.12741315072794576No Hit
ATGGCGGGGACTGAAGTATGTATCATTAAAGTTGGTCATCAACACTTGTTAA2090.1262054431381074No Hit
ATGGCGGGGCATGCACAGCACACACATCCATGAAATGAAAATCACTAAAGCA2050.12379002795843069No Hit
ATGGCGGGATTCTCCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCTT1980.11956305139399648No Hit
ATGGCGGGGTGAAGGAAGAAGAGGAGAAGGAGGAAAGGAAGAGGAAGAGAAA1960.11835534380415814No Hit
ATGGCGGGGGTGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGAGTAAAC1940.11714763621431978No Hit
ATGGCGGGGAATTCTAGAATTTGCAGTAGCATTAATTCAAGTCCTACGTATT1940.11714763621431978No Hit
ATGGCGGGGATTGGACCCAATGGCTCTGGTAAGTCAAATCTCATGGATGCCA1930.11654378241940061No Hit
ATGGCGGGGGATATGTAATCCCATCGTCACTCGCAGGAAGCGTAGTTAGAAT1910.11533607482956228No Hit
ATGGCGAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG1900.11473222103464309No Hit
ATGGCGGGGCACTTCGCCATCATATTCGTAGGAGTAAACATAACATTCTTCC1880.11352451344480476No Hit
ATGGCGGGGCGCCAACTTCGCCGCCAACTTCGGTGCCATTGGCTTCTTCTGG1850.11171295206004722No Hit
ATGGCGGGGATCAGGTGACTTTCTGTTGACCACTCTCTGAATAGGCTTCTGT1840.11110909826512806No Hit
ATGGCGGGACATCCCTTTCAGAGAGCCAGGCCACCACCAGGGCCCCTGGCCC1830.11050524447020887No Hit
ATGGCGACCATCCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCGGA1830.11050524447020887No Hit
ATGGCGGGGTGCTCTAAATTTATAGCTGTCTAATGTGCTTAGTGTGTATCTG1830.11050524447020887No Hit
ATGGCGGGGATCCTTGATGTTTGCCGCCTGGCGGCTGAGCCCTTAGCCTCGC1820.1099013906752897No Hit
ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC1820.1099013906752897No Hit
ATGGCGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCGCTGACTGCTCTTC1810.10929753688037051No Hit
ATGGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1810.10929753688037051No Hit
ATGGCGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1800.10869368308545135No Hit
ATGGCGGGGAAGGAGATCTACACGCACTTCACGTGCGCCACCGACACCAAGA1800.10869368308545135No Hit
ATGGCGGAGAGAGACAGAGAGACAGAGAGACAGAGAGACAGAGAGAGAAGGC1790.10808982929053218No Hit
ATGGCGGGGTCGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAGGAAGGA1780.107485975495613No Hit
ATGGCGGGGTGCAGTACCCCATAGACTGGTGTTAAGTGTTGTCTACAGTGCA1780.107485975495613No Hit
ATGGCGGGGGATGAGCAGCTTATTAATAGAAATAAACATGTGCAGCAGCGAC1770.10688212170069382No Hit
ATGGCGGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCCTGACCCGCC1770.10688212170069382No Hit
ATGGCGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1770.10688212170069382No Hit
ATGGCGGGGACTCCAGTGTCTTGTACTGACTGCTGCCAATCTGTGAAAATGA1750.10567441411085549No Hit
ATGGCGGGGATCCCTTGACATCGTGCTTCACGATACATACTATGTAGTAGCC1740.1050705603159363No Hit
ATGGCGGGGAGAACCAGTAAGCACAAATAGAAAATATCACACACATGTGGGA1730.10446670652101712No Hit
ATGGCGGGGATTAGCTCTCGGGAGAACATCTTGGTTTCCATGGGAGCTGTGG1720.10386285272609797No Hit
ATGGCGGGGGAGAAAGAAAGAAGGGAAGAGAGAGAGAGAGAGAGAGAGAGGG1720.10386285272609797No Hit
ATGGCGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA1710.10325899893117879No Hit
ATGGCGAGGCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATAAT1710.10325899893117879No Hit
ATGGCGGGGGCAGGGGTCAGAGGGGATGGTGCCCCAGGATCATGAGCATCCG1700.1026551451362596No Hit
ATGGCGGGGTACAGAAATCATTGAAAAACTTCGTATAAATCGTATTGCTTGC1690.10205129134134043No Hit
ATGGCGGGGGGGGAGGGCTGCCTGAGAAACACCGTGTACGGGGACGACAGCG1670.10084358375150208No Hit
ATGGCGGGGATTTGATCCTACCAAAACCACTGCTGCTGTTTCTGAGAAAAGA1660.10023972995658291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCGG351.0124859E-746.00000432
GAAGCGT351.0124859E-746.00000437
AATCACA351.0124859E-746.00000413
CATGCTC351.0124859E-746.00000443
GCGGTGA351.0124859E-746.0000044
GGACTAA351.0124859E-746.0000048
ACATTCG351.0124859E-746.00000436
CCGTGAA351.0124859E-746.00000432
AAGCGTA351.0124859E-746.00000438
AATATCA351.0124859E-746.00000433
GTAATCC351.0124859E-746.00000415
CCGTAGA351.0124859E-746.00000426
CGTAGAC351.0124859E-746.00000427
TCTCGAA351.0124859E-746.00000411
TCCACGC351.0124859E-746.00000439
GGTATGC351.0124859E-746.0000048
TACTCTC351.0124859E-746.00000435
GGTGCGA351.0124859E-746.00000434
TGCTCAT351.0124859E-746.00000446
CTCGAAC351.0124859E-746.00000412
Produced by FastQC (version 0.11.5)