FastQCFastQC Report
Fri 17 Jun 2016
SRR1527673_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527673_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168289
Sequences flagged as poor quality0
Sequence length52
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19021.1301986463761742No Hit
ATGGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14180.8425981496116799No Hit
ATGGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10050.5971869819180101No Hit
ATGGCGGGGATCCAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGAAAAGG8470.5033008693378652No Hit
ATGGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7720.4587346766574167No Hit
ATGGCGAGGGGCGCATTGCTGCACCTCCAGGGCTGGAGAGCTTCCTTGATGC6520.3874287683686991No Hit
ATGGCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG4500.2673971560826911No Hit
ATGGCGGGGGGGAGGGCTGCCTGAGAAACACCGTGTACGGGGACGACAGCGG4410.2620492129610373No Hit
ATGGCGGGGCGACGACGATGATGATGACGACGATGATGAAGATGACGACGAC4180.24838224720569974No Hit
ATGGCGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4010.2382805768647981No Hit
ATGGCGAGGCGCTTGGTGGTAGGGGCACTCACCTTTAATCCAAGGACTGGAG3160.18777222516028974No Hit
ATGGCGGGGAACAAAACAAAACAAAAACCAAAAGGATGCATTATCATCTTGG3090.18361271384344788No Hit
ATGGCGGGGCGCAGGGCCAAGGGCATGTTCACCGCTGAAGACCTGCGCTAGG3080.18301849794104189No Hit
ATGGCGGGGCACATCACCTGCTCTACCAACTGGTTAGAAACAATAGTCTCAC3060.18183006613622996No Hit
ATGGCGGGGGGGTGGGATTTTTCCTTTATTATGTGCAACTGTAGTTATTTGT3010.17885898662420005No Hit
ATGGCGGGGCATTCGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAGGAA3000.17826477072179406No Hit
ATGGCGGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTATC2960.17588790711217012No Hit
ATGGCGGTGACTTTGTAGAGATTGAAGTGGAGGAGTTAAGACACATTGACTG2820.16756888447848642No Hit
ATGGCGGGGGATAATCACAGGAAAAGATGCCTTCTAAAGACTTGGGCATAAA2820.16756888447848642No Hit
ATGGCGGGGGTTTGCTGGCTATACAGACAACTTGGTGCGAGTATGGCAGGGA2780.16519202086886248No Hit
ATGGCGGGGGTCTTGTGTAACTTGATGTACATTTTAAATGGCCAGCCCAGCA2760.16400358906405052No Hit
ATGGCGGGGAGAAACTGCCAGCCGAGATCACAAGCATTGGGGCTGAAGCAGG2590.15390191872314887No Hit
ATGGCGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG2570.15271348691833692No Hit
ATGGCGGGGGCTTGCAGCTGCCCCACAGTGCTTGACCTCTGAGTCATTGGTT2450.14558289608946515No Hit
ATGGCGGGGGGGGGGGATTTTTCCTTTATTATGTGCAACTGTAGTTATTTGT2390.14201760067502925No Hit
ATGGCGGGGGTTCAGGAAGAATATACTCAGTGTCTGTCTCCACGCTGCTTTA2390.14201760067502925No Hit
ATGGCGAGGCACTCCTCCAGGTTCTTTCAGATTCTCCCTGTTACCTACCCAA2360.14023495296781133No Hit
ATGGCGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2270.1348870098461575No Hit
ATGGCGGGGTGCTGGGTCTCACAGTATCCCCAAGGGGTGCACACTGGGTGAG2250.13369857804134555No Hit
ATGGCGGGGGAGGGCTGGCGAGATGGCTCAGAGAGTAAGAACACTGACTGCT2250.13369857804134555No Hit
ATGGCGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGG2250.13369857804134555No Hit
ATGGCGGGGTGCCTAAAGATGTAAACAATAGAGCACGCTCTGCGCTTTGGCG2200.13072749852931564No Hit
ATGGCGAGGGGAGTCCCAGGGCACACAGCTCATCTCGATCCTTGTGGGTTGG2140.12716220311487975No Hit
ATGGCGGGGACTGAAGTATGTATCATTAAAGTTGGTCATCAACACTTGTTAA2130.12656798721247378No Hit
ATGGCGGGGACTCCCTTGTACATTTCTGCCACTTCAGTAAAAAGATGCTCAT2130.12656798721247378No Hit
ATGGCGGGGGTGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGAGTAAAC2120.12597377131006782No Hit
ATGGCGGGGAACAGCGACGGCACCATCGACTTCCGGGAGTTCATCATTGCTC2020.120031612286008No Hit
ATGGCGGGGCACTTCGCCATCATATTCGTAGGAGTAAACATAACATTCTTCC2010.11943739638360203No Hit
ATGGCGGGGTTCATGGCTGCCCTCCACCATATCACACATTCGAGGAACCAAC2010.11943739638360203No Hit
ATGGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC1990.11824896457879004No Hit
ATGGCGGGGTGCAGTACCCCATAGACTGGTGTTAAGTGTTGTCTACAGTGCA1960.1164663168715721No Hit
ATGGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.11349523735954223No Hit
ATGGCGGGATTCTCCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCTT1900.11290102145713624No Hit
ATGGCGAGGCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATAAT1900.11290102145713624No Hit
ATGGCGGAGTGAACAAGACGCTAACCCGTAGACAACCGTCAGCATGCTCGCT1850.10992994194510633No Hit
ATGGCGGGGAATTCTAGAATTTGCAGTAGCATTAATTCAAGTCCTACGTATT1850.10992994194510633No Hit
ATGGCGGGGATCAGGTGACTTTCTGTTGACCACTCTCTGAATAGGCTTCTGT1840.10933572604270037No Hit
ATGGCGGAGAGAGACAGAGAGACAGAGAGACAGAGAGACAGAGAGAGAAGGC1830.10874151014029437No Hit
ATGGCGGGGACTCCAGTGTCTTGTACTGACTGCTGCCAATCTGTGAAAATGA1820.1081472942378884No Hit
ATGGCGGGGAGCCTTTCCAAAGAGCCTAGCAAACCCAAGACAGGAAAGGCTG1820.1081472942378884No Hit
ATGGCGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1800.10695886243307644No Hit
ATGGCGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790.10636464653067046No Hit
ATGGCGACCATCCAAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCGGA1790.10636464653067046No Hit
ATGGCGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCGCTGACTGCTCTTC1780.10577043062826447No Hit
ATGGCGGGGCATGCACAGCACACACATCCATGAAATGAAAATCACTAAAGCA1770.1051762147258585No Hit
ATGGCGGGGTGAAGGAAGAAGAGGAGAAGGAGGAAAGGAAGAGGAAGAGAAA1770.1051762147258585No Hit
ATGGCGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA1740.10339356701864054No Hit
ATGGCGGGGAGCCCAGGAGGAGTACATCAAGCGCGCCCTGGCCAACAGCCTC1720.1022051352138286No Hit
ATGGCGGGGTGCTCTAAATTTATAGCTGTCTAATGTGCTTAGTGTGTATCTG1710.1016109193114226No Hit
ATGGCGGGGTATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCC1690.10042248750661066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAT206.296029E-446.020
CTTCGTA301.8518786E-646.029
TATTTCC206.296029E-446.036
ACAACGC453.074092E-1046.038
CAGAATT206.296029E-446.030
AACCGTT206.296029E-446.017
CTAGACC206.296029E-446.013
AAGTACC206.296029E-446.015
GATACTC206.296029E-446.011
TAATACT206.296029E-446.040
AGGTACT206.296029E-446.037
AACCGCC206.296029E-446.022
CCTTAGT253.404648E-546.017
AATCCGC501.6370905E-1146.011
TCGTTCG453.074092E-1046.046
ATCTCGG301.8518786E-646.010
GGTACAC206.296029E-446.08
CGAACCA405.5624696E-946.014
CGTATTG301.8518786E-646.041
TTAATTG206.296029E-446.024
Produced by FastQC (version 0.11.5)