FastQCFastQC Report
Fri 17 Jun 2016
SRR1527672_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527672_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599818
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC101971.700015671420331No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG51260.854592559743122No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT23510.39195222550840425No Hit
CCGACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC18670.31126108252836693No Hit
CCGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT17850.29759026904827796No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG15480.25807828374606967No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCAACTG14890.24824196672990806No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGGA12870.21456508474237188No Hit
CCGACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT12010.20022740231203465No Hit
CCGACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC11550.19255840938417987No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT11150.18588971988169745No Hit
CCGACGGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGC10850.18088820275483564No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT10700.17838744419140473No Hit
CCGACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA9660.16104885148495043No Hit
CCGACGGGGGGGCTGGAGAGATGGCTTAGCGGTAAAGAGCAATGACTGCT9560.15938167910932985No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC9050.15087909999366475No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC9010.1502122310434165No Hit
CCGACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC8890.14821162419267178No Hit
CCGACGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCTCCGACTGCT8870.14787818971754765No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCT8690.14487727944143056No Hit
CCGACGGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGC8610.1435435415409341No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCT8340.13904217612675845No Hit
CCGACGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA8300.1383753071765102No Hit
CCGACGGGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG8270.137875155463824No Hit
CCGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC7760.12937257634815896No Hit
CCGACGGGGGGGGGCTGGCGAGATGGCTCAGTGGATAAGAGCACTGACTG7650.1275386867349763No Hit
CCGACGGGGGGGCCTCAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT7330.12220373513299033No Hit
CCGACGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC7330.12220373513299033No Hit
CCGACGGGGAGCCGGGCGGTGGTGGCACACGCCTTTAATCCCAGCACTTG7240.1207032799949318No Hit
CCGACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA7130.11886939038174914No Hit
CCGACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC7100.11836923866906295No Hit
CCGACGGGGGGCTGGGAGGTGGTGGCGCACGCCTTTAATCCCAGCACTTG6850.11420130773001143No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC6820.11370115601732525No Hit
CCGACGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA6780.11303428706707702No Hit
CCGACGGGGGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCAGTGAAT6430.1071991837524049No Hit
CCGACGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT6280.10469842518897399No Hit
CCGACGGGGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCAGAC6240.10403155623872574No Hit
CCGACGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA6100.10169751491285689No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC6040.10069721148748453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATG207.853467E-444.043
TTCTACG302.525685E-644.031
AGCGTAC207.853467E-444.012
GTACCGC207.853467E-444.039
ACCGGTA207.853467E-444.019
CGAACGT207.853467E-444.043
CTCGTTA351.4445322E-744.024
CTACTAA454.783942E-1044.024
ACGCATA254.440287E-544.012
CTATCGA254.440287E-544.034
TAGCGTC207.853467E-444.012
GACGTAA454.783942E-1044.020
TAGCGAT207.853467E-444.044
ACCGAAG408.301868E-944.032
CAACCCG207.853467E-444.038
GATCATA254.440287E-544.09
GTTAGCG207.853467E-444.010
TCGATTG254.440287E-544.016
CGCGAAA254.440287E-544.011
TCTAGAC207.853467E-444.044
Produced by FastQC (version 0.11.5)