##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527672_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	599818
Sequences flagged as poor quality	0
Sequence length	50
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.57073145520808	34.0	31.0	34.0	31.0	34.0
2	33.02550940451937	34.0	31.0	34.0	31.0	34.0
3	33.07067810569206	34.0	33.0	34.0	31.0	34.0
4	36.26203615096579	37.0	37.0	37.0	35.0	37.0
5	36.451765368828546	37.0	37.0	37.0	35.0	37.0
6	36.35411408127132	37.0	37.0	37.0	35.0	37.0
7	36.62557309050412	37.0	37.0	37.0	35.0	37.0
8	36.68318223194369	37.0	37.0	37.0	35.0	37.0
9	38.66606704033557	39.0	39.0	39.0	39.0	39.0
10	38.13768843215775	39.0	39.0	39.0	37.0	39.0
11	36.76372332940992	39.0	35.0	39.0	34.0	39.0
12	36.57205519007432	39.0	35.0	39.0	33.0	39.0
13	36.43563047457729	39.0	35.0	39.0	33.0	39.0
14	37.098276477198084	40.0	35.0	40.0	33.0	40.0
15	37.1703183298934	40.0	35.0	40.0	33.0	40.0
16	37.118204188603876	39.0	35.0	40.0	33.0	40.0
17	36.943781280321694	39.0	35.0	40.0	32.0	40.0
18	36.82890476777956	39.0	35.0	40.0	32.0	40.0
19	36.63793350649697	39.0	35.0	40.0	32.0	40.0
20	36.540302225008254	39.0	35.0	40.0	31.0	40.0
21	36.56475464224148	39.0	35.0	40.0	31.0	40.0
22	36.6484833732899	39.0	35.0	40.0	32.0	40.0
23	36.66501672173893	38.0	35.0	40.0	32.0	40.0
24	36.70486047434388	38.0	35.0	40.0	33.0	40.0
25	36.676156767552825	38.0	35.0	40.0	33.0	40.0
26	36.50192725126622	38.0	35.0	40.0	32.0	40.0
27	36.37332157421084	38.0	35.0	40.0	32.0	40.0
28	36.13885711999306	38.0	35.0	40.0	31.0	40.0
29	36.2100703880177	38.0	35.0	40.0	32.0	40.0
30	36.25577258435059	38.0	35.0	40.0	32.0	40.0
31	36.24968907235195	37.0	35.0	40.0	32.0	40.0
32	36.18324391732159	37.0	35.0	40.0	32.0	40.0
33	35.998189450800076	37.0	35.0	40.0	32.0	40.0
34	35.543598224794856	36.0	35.0	40.0	30.0	40.0
35	35.51809048744786	36.0	35.0	40.0	31.0	40.0
36	35.60055383466318	36.0	35.0	40.0	31.0	40.0
37	35.51385753678616	35.0	35.0	40.0	31.0	40.0
38	35.455876615906824	35.0	35.0	40.0	31.0	40.0
39	35.31103101274053	35.0	35.0	40.0	31.0	40.0
40	35.13136984885415	35.0	35.0	39.0	31.0	40.0
41	34.97257501442104	35.0	34.0	39.0	31.0	40.0
42	34.90553801319734	35.0	34.0	39.0	30.0	40.0
43	34.78814907188514	35.0	34.0	39.0	30.0	40.0
44	34.50677538853452	35.0	34.0	39.0	30.0	40.0
45	34.4632771940822	35.0	34.0	38.0	30.0	40.0
46	34.31377351129843	35.0	34.0	37.0	29.0	40.0
47	34.26785791690146	35.0	34.0	37.0	29.0	40.0
48	34.22040185522942	35.0	34.0	37.0	29.0	40.0
49	34.12057324054963	35.0	34.0	37.0	29.0	40.0
50	33.57458262339576	35.0	33.0	36.0	27.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	8.0
16	26.0
17	87.0
18	160.0
19	295.0
20	489.0
21	814.0
22	1188.0
23	1580.0
24	2299.0
25	3201.0
26	4202.0
27	5253.0
28	7122.0
29	8698.0
30	11393.0
31	14422.0
32	19234.0
33	29264.0
34	81427.0
35	75471.0
36	66478.0
37	94262.0
38	119103.0
39	53336.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.31229139505649	0.5723402765505536	0.07035467425118953	0.04501365414175633
8	99.4670049915141	0.3767809568902567	0.06935437082581716	0.08685968076983352
9	95.42444541510925	2.173826060571707	1.2026981517727044	1.199030372546339
10	54.48135934566819	31.266817601339074	5.412641834689856	8.839181218302885
11	43.12591486084112	19.988229763028116	19.128969120633258	17.756886255497502
12	37.0667435788856	13.994244920959359	24.17866752915051	24.760343971004538
13	26.94617367268071	15.001217035834202	27.021529864058763	31.031079427426317
14	25.66961978466802	18.806037831475546	30.081624759510383	25.442717624346052
15	32.16458992561077	17.54965672920786	27.76075409540894	22.52499924977243
16	35.742175126455024	18.970754462186864	24.348052242513564	20.93901816884455
17	33.667379104995184	20.454871310964325	23.53080434398434	22.34694524005615
18	33.30810345804894	20.675604933496494	22.591686144797254	23.424605463657308
19	29.968423755205748	24.745506136861515	22.406630011103367	22.87944009682937
20	33.420637593403335	24.494930128805738	21.267117692366686	20.817314585424246
21	30.003934526806464	23.544808591939557	24.83236581763135	21.618891063622634
22	28.271075559586407	21.071058220993702	27.264103444711562	23.39376277470833
23	30.95672353947364	21.22560510021373	25.583593690085994	22.234077670226636
24	33.08520251142847	21.019709311824585	21.06839074519271	24.826697431554237
25	28.61351276553888	21.057554124751174	21.17675694960805	29.1521761601019
26	27.320787305482664	19.697308183482324	24.819862024814192	28.162042486220823
27	25.257494773414603	19.659463370555734	23.554311474480592	31.52873038154907
28	25.947203985208844	25.458555761914447	20.73679015968177	27.857450093194934
29	31.77580532761604	22.68588138401982	21.522028348599076	24.01628493976506
30	28.073348915837805	20.803310337469032	25.049765095412273	26.07357565128089
31	33.498661260582374	19.789669533091704	23.49812776542218	23.21354144090374
32	36.27650387284143	18.827711072358618	22.965632908648956	21.930152146151
33	33.81225638443661	19.464404202608122	25.153129782700752	21.57020963025451
34	28.1520394519671	22.277757586467896	29.24187003391029	20.32833292765472
35	23.88024367391442	28.430290521458172	28.018332227442322	19.671133577185078
36	26.71043549876796	30.25717801066323	22.00534162029149	21.02704487027732
37	30.959391015274633	27.372469649126902	21.077060041545938	20.59107929405253
38	30.115801793210608	28.75455554851638	19.805174236184975	21.32446842208803
39	30.540430597281176	24.51126841808682	19.89036674457919	25.057934240052816
40	28.254737270305323	22.19173149188587	21.064889683203905	28.4886415546049
41	23.42493889813243	26.789959621085064	20.178787565561553	29.60631391522095
42	23.658009596244195	23.5744842602256	20.731455208079787	32.03605093545042
43	24.424242020079422	20.92184629337566	22.908115461690045	31.74579622485487
44	25.861344607864385	21.727924136988218	23.185699662230878	29.225031592916515
45	27.704403669112963	22.32043719928378	22.200567505476663	27.774591626126593
46	26.92983538339963	25.31951358578769	21.79094325278668	25.959707778026004
47	25.739474307206518	22.271589048678102	24.776348825810494	27.212587818304883
48	27.3157857883558	20.603082935156998	26.5485530610952	25.532578215392004
49	28.712709521888307	20.774301538133233	24.675985048798136	25.837003891180327
50	24.539276913997245	20.8249835783521	26.15109916674725	28.484640340903404
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.0
11	4.0
12	6.0
13	19.0
14	32.0
15	157.0
16	282.0
17	362.0
18	442.0
19	705.0
20	968.0
21	1020.5
22	1073.0
23	941.5
24	810.0
25	1193.5
26	1577.0
27	1520.0
28	1463.0
29	1644.5
30	1826.0
31	2204.5
32	2583.0
33	2862.5
34	3142.0
35	3600.5
36	4059.0
37	4824.0
38	5589.0
39	5872.5
40	6156.0
41	7312.5
42	8469.0
43	9863.0
44	11257.0
45	12225.5
46	13194.0
47	14514.0
48	15834.0
49	16967.5
50	18101.0
51	19820.5
52	21540.0
53	23893.0
54	26246.0
55	27331.5
56	28417.0
57	32657.5
58	36898.0
59	41000.0
60	45102.0
61	48335.5
62	51569.0
63	58482.5
64	65396.0
65	63933.0
66	62470.0
67	60703.0
68	58936.0
69	47431.5
70	35927.0
71	29806.5
72	23686.0
73	20165.0
74	16644.0
75	13997.0
76	11350.0
77	9352.5
78	7355.0
79	6867.0
80	6379.0
81	4832.0
82	3285.0
83	2181.5
84	1078.0
85	743.0
86	408.0
87	314.5
88	221.0
89	126.5
90	32.0
91	21.0
92	10.0
93	6.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	599818.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.95768716510675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.4017546209483	6.811232740597982
2	9.086190731315295	1.8095488964986046
3	3.474082507366729	1.0378148038238266
4	1.6725823734261989	0.6662020812979937
5	0.9024243236003214	0.44930295522975305
6	0.5809670506295205	0.34710528860420997
7	0.46879185641575144	0.32676578562163855
8	0.3432226091615323	0.2734162696017792
9	0.3616394320921511	0.3240983098206456
>10	9.28542727029199	26.826470696111155
>50	3.797214036967586	25.719301521461507
>100	1.5403160996517546	25.719301521461507
>500	0.06362175194213769	4.4751908078783895
>1k	0.018416822930618804	2.659640090827551
>5k	0.0016742566300562553	0.854592559743122
>10k+	0.0016742566300562553	1.700015671420331
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	10197	1.700015671420331	No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	5126	0.854592559743122	No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	2351	0.39195222550840425	No Hit
CCGACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	1867	0.31126108252836693	No Hit
CCGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1785	0.29759026904827796	No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	1548	0.25807828374606967	No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCAACTG	1489	0.24824196672990806	No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGGA	1287	0.21456508474237188	No Hit
CCGACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1201	0.20022740231203465	No Hit
CCGACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	1155	0.19255840938417987	No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	1115	0.18588971988169745	No Hit
CCGACGGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGC	1085	0.18088820275483564	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	1070	0.17838744419140473	No Hit
CCGACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	966	0.16104885148495043	No Hit
CCGACGGGGGGGCTGGAGAGATGGCTTAGCGGTAAAGAGCAATGACTGCT	956	0.15938167910932985	No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	905	0.15087909999366475	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	901	0.1502122310434165	No Hit
CCGACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	889	0.14821162419267178	No Hit
CCGACGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCTCCGACTGCT	887	0.14787818971754765	No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCT	869	0.14487727944143056	No Hit
CCGACGGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGC	861	0.1435435415409341	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCT	834	0.13904217612675845	No Hit
CCGACGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	830	0.1383753071765102	No Hit
CCGACGGGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	827	0.137875155463824	No Hit
CCGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	776	0.12937257634815896	No Hit
CCGACGGGGGGGGGCTGGCGAGATGGCTCAGTGGATAAGAGCACTGACTG	765	0.1275386867349763	No Hit
CCGACGGGGGGGCCTCAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	733	0.12220373513299033	No Hit
CCGACGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC	733	0.12220373513299033	No Hit
CCGACGGGGAGCCGGGCGGTGGTGGCACACGCCTTTAATCCCAGCACTTG	724	0.1207032799949318	No Hit
CCGACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	713	0.11886939038174914	No Hit
CCGACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	710	0.11836923866906295	No Hit
CCGACGGGGGGCTGGGAGGTGGTGGCGCACGCCTTTAATCCCAGCACTTG	685	0.11420130773001143	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC	682	0.11370115601732525	No Hit
CCGACGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA	678	0.11303428706707702	No Hit
CCGACGGGGGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCAGTGAAT	643	0.1071991837524049	No Hit
CCGACGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT	628	0.10469842518897399	No Hit
CCGACGGGGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCAGAC	624	0.10403155623872574	No Hit
CCGACGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	610	0.10169751491285689	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC	604	0.10069721148748453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.001333737900496484	0.0	0.0	0.0	0.0
8	0.002334041325868847	0.0	0.0	0.0	0.0
9	0.012837227292278658	0.0	0.0	0.0	0.0
10	0.028675364860674405	0.0	0.0	0.0	0.0
11	0.04901486784324578	0.0	0.0	0.0	0.0
12	0.06351926751114505	0.0	0.0	0.0	6.66868950248242E-4
13	0.07318886728974455	0.0	0.0	0.0	6.66868950248242E-4
14	0.1055320113767843	0.0	0.0	0.0	6.66868950248242E-4
15	0.12153686618274209	0.0	0.0	0.0	6.66868950248242E-4
16	0.13737500375113784	0.0	0.0	0.0	6.66868950248242E-4
17	0.15371329303221978	0.0	0.0	0.0	6.66868950248242E-4
18	0.16721738927474666	0.0	0.0	0.0	6.66868950248242E-4
19	0.18672330606950774	0.0	0.0	0.0	6.66868950248242E-4
20	0.2050622022013344	0.0	0.0	0.0	6.66868950248242E-4
21	0.21506523645505804	0.0	0.0	0.0	6.66868950248242E-4
22	0.22640200860927814	0.0	0.0	0.0	6.66868950248242E-4
23	0.24240686341523596	0.0	0.0	0.0	6.66868950248242E-4
24	0.26174606297243497	0.0	0.0	0.0	6.66868950248242E-4
25	0.27541687645252394	0.0	0.0	0.0	6.66868950248242E-4
26	0.30659299987662925	0.0	0.0	0.0	6.66868950248242E-4
27	0.3232647236328353	0.0	0.0	0.0	6.66868950248242E-4
28	0.3767809568902567	0.0	0.0	0.0	6.66868950248242E-4
29	0.39011833589522155	0.0	0.0	0.0	6.66868950248242E-4
30	0.41512592152953065	0.0	0.0	0.0	6.66868950248242E-4
31	0.4254623902583784	0.0	0.0	0.0	6.66868950248242E-4
32	0.4426342657272706	0.0	0.0	0.0	6.66868950248242E-4
33	0.4533041689312425	0.0	0.0	0.0	6.66868950248242E-4
34	0.46997589268744855	0.0	0.0	0.0	6.66868950248242E-4
35	0.5184906088180081	0.0	0.0	0.0	6.66868950248242E-4
36	0.5393302635132657	0.0	0.0	0.0	6.66868950248242E-4
37	0.5615036561090198	0.0	0.0	0.0	6.66868950248242E-4
38	0.5800092694784085	0.0	0.0	0.0	6.66868950248242E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATG	20	7.853467E-4	44.0	43
TTCTACG	30	2.525685E-6	44.0	31
AGCGTAC	20	7.853467E-4	44.0	12
GTACCGC	20	7.853467E-4	44.0	39
ACCGGTA	20	7.853467E-4	44.0	19
CGAACGT	20	7.853467E-4	44.0	43
CTCGTTA	35	1.4445322E-7	44.0	24
CTACTAA	45	4.783942E-10	44.0	24
ACGCATA	25	4.440287E-5	44.0	12
CTATCGA	25	4.440287E-5	44.0	34
TAGCGTC	20	7.853467E-4	44.0	12
GACGTAA	45	4.783942E-10	44.0	20
TAGCGAT	20	7.853467E-4	44.0	44
ACCGAAG	40	8.301868E-9	44.0	32
CAACCCG	20	7.853467E-4	44.0	38
GATCATA	25	4.440287E-5	44.0	9
GTTAGCG	20	7.853467E-4	44.0	10
TCGATTG	25	4.440287E-5	44.0	16
CGCGAAA	25	4.440287E-5	44.0	11
TCTAGAC	20	7.853467E-4	44.0	44
>>END_MODULE