FastQCFastQC Report
Fri 17 Jun 2016
SRR1527671_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527671_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901821
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC152191.6875854521019136No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT71810.796277753567504No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT35600.3947568309010325No Hit
CCGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT27100.3005030931858983No Hit
CCGACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC24710.27400115987540763No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCAACTGCT21810.24184400230200892No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT20440.2266525175173344No Hit
CCGACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC17490.19394092619267017No Hit
CCGACGGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTC17120.1898381164333055No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC16470.18263047766685406No Hit
CCGACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT16340.18118894991356377No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGGAAG15660.17364865089635304No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT15190.1684369736344574No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC14590.16178376861927143No Hit
CCGACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA14420.15989869386496877No Hit
CCGACGGGGGGGCTGGAGAGATGGCTTAGCGGTAAAGAGCAATGACTGCTCT13630.15113864059497395No Hit
CCGACGGGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT13610.15091686709446775No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA13520.14991888634218986No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT13280.14725760433611548No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT12930.14337656807725702No Hit
CCGACGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCTCCGACTGCTCT12840.14237858732497913No Hit
CCGACGGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGCTC12690.14071528607118264No Hit
CCGACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTT12670.14049351257067644No Hit
CCGACGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG12550.13916287156763926No Hit
CCGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC11760.13040281829764444No Hit
CCGACGGGGGGGCCTCAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT11290.1251911410357488No Hit
CCGACGGGGGGGGGCTGGCGAGATGGCTCAGTGGATAAGAGCACTGACTGCT10780.11953591677284073No Hit
CCGACGGGGGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCAGTGAATCT10630.11787261551904425No Hit
CCGACGGGGAGCCGGGCGGTGGTGGCACACGCCTTTAATCCCAGCACTTGGG10590.11742906851803184No Hit
CCGACGGGGGGCTGGGAGGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGG10580.11731818176777876No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC10540.11687463476676635No Hit
CCGACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC10070.11166295750487071No Hit
CCGACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT9930.1101105430013273No Hit
CCGACGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA9810.10877990199829012No Hit
CCGACGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTC9780.10844724174753084No Hit
CCGACGGGGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCAGACTG9650.10700571399424054No Hit
CCGACGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG9340.10356822473639446No Hit
CCGACGGGGGGGCTGGCGAGATGGCTCAGCGGTTAAGAGCACCGACTGCTCT9120.1011287162308263No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC9080.1006851692298139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTC351.0192343E-746.00000414
ACGGGTA2850.046.0000044
CAATACG351.0192343E-746.00000418
CTTCGTA206.310729E-446.014
AACGTTG301.8609535E-646.040
CGGGTTA253.416567E-546.040
AACGTAG206.310729E-446.018
GATACTC253.416567E-546.020
TAATACA501.6370905E-1146.017
AGGTACG301.8609535E-646.034
GTCGCCC405.6097633E-946.040
CTCGTCA253.416567E-546.029
GTATTCG301.8609535E-646.044
CGAAACC206.310729E-446.026
ACGTTAC206.310729E-446.037
CTATCGC253.416567E-546.032
CTATCGA301.8609535E-646.034
ACACCGT253.416567E-546.044
GTTTACG253.416567E-546.016
CGTGATA206.310729E-446.018
Produced by FastQC (version 0.11.5)