##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527671_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	901821
Sequences flagged as poor quality	0
Sequence length	52
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2730342274132	33.0	31.0	34.0	31.0	34.0
2	32.73731594185542	34.0	31.0	34.0	31.0	34.0
3	32.76650244338954	34.0	31.0	34.0	31.0	34.0
4	36.119034708661694	37.0	35.0	37.0	35.0	37.0
5	36.27222475413635	37.0	37.0	37.0	35.0	37.0
6	36.1334488773271	37.0	37.0	37.0	35.0	37.0
7	36.569355781247054	37.0	37.0	37.0	35.0	37.0
8	36.61852074857428	37.0	37.0	37.0	35.0	37.0
9	38.58958706883073	39.0	39.0	39.0	37.0	39.0
10	37.874310977455615	39.0	38.0	39.0	35.0	39.0
11	36.397424766112124	38.0	35.0	39.0	33.0	39.0
12	36.189781564190675	38.0	35.0	39.0	33.0	39.0
13	36.08018997118053	38.0	35.0	39.0	32.0	39.0
14	36.76970485273685	39.0	35.0	40.0	33.0	40.0
15	36.85443009200274	39.0	35.0	40.0	33.0	40.0
16	36.81182074935048	39.0	35.0	40.0	33.0	40.0
17	36.689805404841984	39.0	35.0	40.0	32.0	40.0
18	36.65723020422013	39.0	35.0	40.0	32.0	40.0
19	36.47979809740514	38.0	35.0	40.0	31.0	40.0
20	36.482762100239405	38.0	35.0	40.0	31.0	40.0
21	36.470536836024	38.0	35.0	40.0	31.0	40.0
22	36.45909443226538	38.0	35.0	40.0	31.0	40.0
23	36.45732246199634	38.0	35.0	40.0	31.0	40.0
24	36.491099674990934	38.0	35.0	40.0	32.0	40.0
25	36.46200631832703	38.0	35.0	40.0	32.0	40.0
26	36.27437928369377	38.0	35.0	40.0	31.0	40.0
27	36.10867234185054	38.0	35.0	40.0	31.0	40.0
28	35.95100468940067	37.0	35.0	40.0	31.0	40.0
29	35.836704844974776	37.0	35.0	40.0	31.0	40.0
30	35.54219961611007	37.0	34.0	40.0	30.0	40.0
31	35.40108624660548	36.0	34.0	40.0	30.0	40.0
32	35.27483502823731	36.0	34.0	40.0	30.0	40.0
33	35.1107547950203	35.0	34.0	40.0	29.0	40.0
34	34.73968448284083	35.0	33.0	40.0	27.0	40.0
35	34.647097372982	35.0	33.0	39.0	27.0	40.0
36	34.80428821240579	35.0	34.0	39.0	29.0	40.0
37	34.70429608536506	35.0	34.0	39.0	29.0	40.0
38	34.6421041426181	35.0	34.0	39.0	29.0	40.0
39	34.495058331974974	35.0	34.0	39.0	29.0	40.0
40	34.286662209019305	35.0	34.0	39.0	28.0	40.0
41	34.11981646025098	35.0	33.0	38.0	27.0	40.0
42	34.06113408314954	35.0	33.0	38.0	27.0	40.0
43	33.90951641179347	35.0	33.0	38.0	27.0	40.0
44	33.78113395008544	35.0	33.0	37.0	27.0	40.0
45	33.64738456966516	35.0	33.0	37.0	26.0	40.0
46	33.453810678615824	35.0	33.0	37.0	25.0	40.0
47	33.35615715313793	35.0	33.0	37.0	25.0	40.0
48	33.216866761807495	35.0	33.0	36.0	25.0	40.0
49	33.52171772447082	35.0	33.0	36.0	27.0	40.0
50	33.597590874464004	35.0	33.0	36.0	27.0	39.0
51	33.6368447840536	35.0	34.0	36.0	28.0	39.0
52	33.15318450113714	35.0	33.0	35.0	26.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	16.0
16	58.0
17	111.0
18	298.0
19	539.0
20	916.0
21	1531.0
22	2390.0
23	3619.0
24	5143.0
25	6782.0
26	9038.0
27	11911.0
28	14538.0
29	17800.0
30	21598.0
31	27709.0
32	36687.0
33	56823.0
34	131757.0
35	106132.0
36	108222.0
37	137264.0
38	155391.0
39	45545.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.35330847252392	0.5650788792897926	0.03803415533681296	0.04357849284946791
8	99.53760225144458	0.3461884342901751	0.0451309073530113	0.07107840691223646
9	95.46617344240154	2.1677250806978328	1.1913672447192958	1.174734232181331
10	54.28416503940361	31.489286676624296	5.398188775821366	8.828359508150731
11	43.08948228085174	20.121177040676585	19.050898127233676	17.738442551237995
12	37.12177915573046	14.02772834076829	24.188835700211015	24.66165680329023
13	27.107485853622837	14.963279852653685	26.7966702926634	31.132564001060075
14	25.626149756991683	19.041140093211403	29.936983059831164	25.395727089965746
15	32.42284222700514	17.433503988041974	27.667020395399973	22.476633389552916
16	35.77982770416745	18.819033932454445	24.562967595564974	20.838170767813125
17	33.711346264946144	20.36956336124353	23.52473495294521	22.394355420865118
18	33.57451201513382	20.691800257479034	22.45922416976318	23.27446355762396
19	29.962708785889884	24.715769537413742	22.53407272618402	22.787448950512353
20	33.45907890812035	24.454298580316937	21.209641381161006	20.87698113040171
21	30.035894041056928	23.507103959654966	24.951514768451833	21.505487230836273
22	28.127089522200084	21.010710551206945	27.28601352153033	23.576186405062646
23	30.99284669574117	21.290810482346277	25.52036379725023	22.19597902466232
24	32.95864700422811	21.1503169697756	21.10274655391702	24.78828947207927
25	28.386564517792333	21.08522644737703	21.187685804610894	29.340523230219745
26	27.386144257008876	19.70690414173101	24.830426437175447	28.07652516408467
27	25.16829836519664	19.67862802041647	23.5316099314609	31.62146368292599
28	25.915675061902526	25.639456167022058	20.586679618239096	27.858189152836317
29	31.990938334769314	22.47319590029507	21.558158437206494	23.977707327729117
30	28.121545184687424	20.881749260662595	24.99553680830231	26.00116874634767
31	33.70569104068324	19.713779120246702	23.452769452030946	23.12776038703911
32	36.24898954448832	18.776786080608016	23.011440186023613	21.962784188880054
33	34.035246462435445	19.50043301275974	25.029468153879762	21.43485237092505
34	28.106797246903763	22.208841887691683	29.13249968674493	20.55186117865962
35	23.770349104755823	28.42005231636877	28.050577664525445	19.759020914349964
36	26.849452385783874	30.20566165569442	21.938832650825386	21.00605330769632
37	31.204973048975347	27.125338620413586	21.090105464388166	20.579582866222896
38	30.203998354440625	28.869698088645084	19.709232763486323	21.217070793427965
39	30.66384570774023	24.49920771416944	19.890199939899382	24.94674663819095
40	28.211806999393453	22.246765156278244	21.062938210576156	28.47848963375215
41	23.372265671347197	26.925077149456488	20.101882746132546	29.60077443306377
42	23.624754801673504	23.45598516778829	20.72384652830218	32.19541350223603
43	24.417040632231895	20.847152594583626	22.900775209271018	31.835031563913457
44	25.61683526997043	21.695214460519328	23.267699465858524	29.420250803651722
45	27.798975628201163	22.265061470070002	22.193983063157766	27.74197983857107
46	26.85422051604476	25.301584239000867	21.783812973971553	26.060382270982824
47	25.953820103989596	22.132440916767298	24.632382701223413	27.281356278019697
48	27.44214206588669	20.440974428406523	26.67092471787639	25.445958787830403
49	28.92126042751278	20.682153110207015	24.608098502917986	25.78848795936222
50	24.401072940195448	20.84803968858565	26.23380914837867	28.51707822284023
51	24.53380438024841	20.862898513119564	27.088856879580312	27.51444022705171
52	23.893322510786508	21.615376000337097	26.637326032549698	27.8539754563267
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	2.5
12	4.0
13	31.5
14	220.5
15	382.0
16	508.0
17	634.0
18	1015.0
19	1396.0
20	1388.5
21	1381.0
22	1195.0
23	1009.0
24	1501.5
25	1994.0
26	1938.0
27	1882.0
28	1935.5
29	1989.0
30	2919.0
31	3849.0
32	3677.0
33	3505.0
34	4388.0
35	5271.0
36	6078.5
37	6886.0
38	7470.5
39	9157.0
40	10259.0
41	12347.0
42	14435.0
43	15310.0
44	16185.0
45	18704.5
46	21224.0
47	21257.5
48	21291.0
49	23814.0
50	26337.0
51	28902.5
52	31468.0
53	35377.0
54	39286.0
55	40758.5
56	42231.0
57	47146.5
58	52062.0
59	59767.5
60	67473.0
61	72783.5
62	78094.0
63	83972.0
64	90054.5
65	90259.0
66	88921.5
67	87584.0
68	70796.0
69	54008.0
70	49231.5
71	44455.0
72	34428.5
73	24402.0
74	21275.5
75	18149.0
76	16031.0
77	13913.0
78	11973.0
79	10033.0
80	8385.5
81	6738.0
82	4524.0
83	2310.0
84	1610.5
85	911.0
86	701.5
87	492.0
88	271.0
89	34.0
90	18.0
91	11.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	901821.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.517433060441041
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.47758162787065	5.832532176562754
2	9.657345206478155	1.6451158267549768
3	4.249336041243556	1.0858030584783456
4	2.107743581732021	0.7181025946390692
5	1.2550122376711972	0.5344741362199372
6	0.6600531167005155	0.3373174942699272
7	0.4921106077175441	0.29340634116969994
8	0.38926209446440657	0.26524110660541284
9	0.278602301723689	0.2135678809874687
>10	5.384575326771858	13.089404660126567
>50	4.461542467322814	27.173574356773685
>100	2.4735718377336875	36.99204165793433
>500	0.07160339530281727	4.224785184643072
>1k	0.03905639743790033	5.110770319165333
>5k	0.0013018799145966776	0.796277753567504
>10k+	0.0013018799145966776	1.6875854521019136
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	15219	1.6875854521019136	No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	7181	0.796277753567504	No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	3560	0.3947568309010325	No Hit
CCGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2710	0.3005030931858983	No Hit
CCGACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	2471	0.27400115987540763	No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCAACTGCT	2181	0.24184400230200892	No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	2044	0.2266525175173344	No Hit
CCGACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1749	0.19394092619267017	No Hit
CCGACGGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTC	1712	0.1898381164333055	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1647	0.18263047766685406	No Hit
CCGACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1634	0.18118894991356377	No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGGAAG	1566	0.17364865089635304	No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	1519	0.1684369736344574	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1459	0.16178376861927143	No Hit
CCGACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1442	0.15989869386496877	No Hit
CCGACGGGGGGGCTGGAGAGATGGCTTAGCGGTAAAGAGCAATGACTGCTCT	1363	0.15113864059497395	No Hit
CCGACGGGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1361	0.15091686709446775	No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1352	0.14991888634218986	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT	1328	0.14725760433611548	No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT	1293	0.14337656807725702	No Hit
CCGACGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCTCCGACTGCTCT	1284	0.14237858732497913	No Hit
CCGACGGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGCTC	1269	0.14071528607118264	No Hit
CCGACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTT	1267	0.14049351257067644	No Hit
CCGACGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	1255	0.13916287156763926	No Hit
CCGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1176	0.13040281829764444	No Hit
CCGACGGGGGGGCCTCAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	1129	0.1251911410357488	No Hit
CCGACGGGGGGGGGCTGGCGAGATGGCTCAGTGGATAAGAGCACTGACTGCT	1078	0.11953591677284073	No Hit
CCGACGGGGGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCAGTGAATCT	1063	0.11787261551904425	No Hit
CCGACGGGGAGCCGGGCGGTGGTGGCACACGCCTTTAATCCCAGCACTTGGG	1059	0.11742906851803184	No Hit
CCGACGGGGGGCTGGGAGGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGG	1058	0.11731818176777876	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	1054	0.11687463476676635	No Hit
CCGACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1007	0.11166295750487071	No Hit
CCGACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT	993	0.1101105430013273	No Hit
CCGACGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	981	0.10877990199829012	No Hit
CCGACGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTC	978	0.10844724174753084	No Hit
CCGACGGGGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCAGACTG	965	0.10700571399424054	No Hit
CCGACGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG	934	0.10356822473639446	No Hit
CCGACGGGGGGGCTGGCGAGATGGCTCAGCGGTTAAGAGCACCGACTGCTCT	912	0.1011287162308263	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC	908	0.1006851692298139	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	4.4354700101239603E-4	0.0	0.0	0.0	0.0
8	0.0016633012537964852	0.0	0.0	0.0	0.0
9	0.008316506268982426	0.0	0.0	0.0	0.0
10	0.021955576550113605	0.0	0.0	0.0	0.0
11	0.04213696509617762	0.0	0.0	0.0	0.0
12	0.05122967861693174	0.0	0.0	0.0	0.00110886750253099
13	0.058880864384395576	0.0	0.0	0.0	0.00110886750253099
14	0.08893117370298541	0.0	0.0	0.0	0.00110886750253099
15	0.10401177173740687	0.0	0.0	0.0	0.00110886750253099
16	0.11842704927030974	0.0	0.0	0.0	0.00110886750253099
17	0.1352818353087808	0.0	0.0	0.0	0.00110886750253099
18	0.14592696333307828	0.0	0.0	0.0	0.00110886750253099
19	0.16477771087610513	0.0	0.0	0.0	0.00110886750253099
20	0.1787494414079956	0.0	0.0	0.0	0.00110886750253099
21	0.18950545618254622	0.0	0.0	0.0	0.00110886750253099
22	0.19959615045557821	0.0	0.0	0.0	0.00110886750253099
23	0.2115719194829129	0.0	0.0	0.0	0.00110886750253099
24	0.22953557302391495	0.0	0.0	0.0	0.00110886750253099
25	0.24184400230200895	0.0	0.0	0.0	0.00110886750253099
26	0.2650193331049066	0.0	0.0	0.0	0.00110886750253099
27	0.2809870251413529	0.0	0.0	0.0	0.00110886750253099
28	0.32911187475119785	0.0	0.0	0.0	0.00110886750253099
29	0.33953522927498914	0.0	0.0	0.0	0.00110886750253099
30	0.36393031433067097	0.0	0.0	0.0	0.00110886750253099
31	0.37524076285648705	0.0	0.0	0.0	0.00110886750253099
32	0.39298264289698287	0.0	0.0	0.0	0.00110886750253099
33	0.4066217131781141	0.0	0.0	0.0	0.00110886750253099
34	0.4227002919648134	0.0	0.0	0.0	0.00110886750253099
35	0.46605701131377514	0.0	0.0	0.0	0.00110886750253099
36	0.4822464768507276	0.0	0.0	0.0	0.00110886750253099
37	0.5008754508932483	0.0	0.0	0.0	0.00110886750253099
38	0.5163995959286821	0.0	0.0	0.0	0.00110886750253099
39	0.5290406854575354	0.0	0.0	0.0	0.00110886750253099
40	0.5372463049762647	0.0	0.0	0.0	0.00110886750253099
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTTC	35	1.0192343E-7	46.000004	14
ACGGGTA	285	0.0	46.000004	4
CAATACG	35	1.0192343E-7	46.000004	18
CTTCGTA	20	6.310729E-4	46.0	14
AACGTTG	30	1.8609535E-6	46.0	40
CGGGTTA	25	3.416567E-5	46.0	40
AACGTAG	20	6.310729E-4	46.0	18
GATACTC	25	3.416567E-5	46.0	20
TAATACA	50	1.6370905E-11	46.0	17
AGGTACG	30	1.8609535E-6	46.0	34
GTCGCCC	40	5.6097633E-9	46.0	40
CTCGTCA	25	3.416567E-5	46.0	29
GTATTCG	30	1.8609535E-6	46.0	44
CGAAACC	20	6.310729E-4	46.0	26
ACGTTAC	20	6.310729E-4	46.0	37
CTATCGC	25	3.416567E-5	46.0	32
CTATCGA	30	1.8609535E-6	46.0	34
ACACCGT	25	3.416567E-5	46.0	44
GTTTACG	25	3.416567E-5	46.0	16
CGTGATA	20	6.310729E-4	46.0	18
>>END_MODULE