FastQCFastQC Report
Fri 17 Jun 2016
SRR1527670_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527670_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911250
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC145571.5974759945130317No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT68720.7541289437585734No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT33590.3686145404663923No Hit
CCGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT26190.2874074074074074No Hit
CCGACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC23130.2538271604938272No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCAACTGCT20820.22847736625514403No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT20490.2248559670781893No Hit
CCGACGGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTC17160.18831275720164609No Hit
CCGACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC16540.18150891632373115No Hit
CCGACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT15520.17031550068587104No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC14980.16438957475994515No Hit
CCGACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA14510.1592318244170096No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT13970.15330589849108367No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGGAAG13950.1530864197530864No Hit
CCGACGGGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT13180.14463648834019205No Hit
CCGACGGGGGGGCTGGAGAGATGGCTTAGCGGTAAAGAGCAATGACTGCTCT12970.14233196159122086No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC12930.14189300411522635No Hit
CCGACGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCTCCGACTGCTCT12610.13838134430727023No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT12570.13794238683127572No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA12540.13761316872427984No Hit
CCGACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTT12310.13508916323731138No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT12050.13223593964334704No Hit
CCGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC11910.13069958847736626No Hit
CCGACGGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGCTC11780.1292729766803841No Hit
CCGACGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG11290.12389574759945131No Hit
CCGACGGGGGGGGGCTGGCGAGATGGCTCAGTGGATAAGAGCACTGACTGCT10420.11434842249657064No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC10340.11347050754458163No Hit
CCGACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT10290.11292181069958848No Hit
CCGACGGGGAGCCGGGCGGTGGTGGCACACGCCTTTAATCCCAGCACTTGGG10290.11292181069958848No Hit
CCGACGGGGGGGCCTCAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT10230.11226337448559671No Hit
CCGACGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA9810.10765432098765432No Hit
CCGACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC9790.10743484224965706No Hit
CCGACGGGGGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCAGTGAATCT9600.10534979423868314No Hit
CCGACGGGGGGCTGGGAGGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGG9490.10414266117969823No Hit
CCGACGGGGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCAGACTG9350.10260631001371742No Hit
CCGACGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTC9150.10041152263374487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA351.0192525E-746.00000431
CTTCGTA206.310763E-446.014
AGGTATG206.310763E-446.041
AGCGTAC501.6370905E-1146.012
AACCGGT206.310763E-446.034
ACCGGTA206.310763E-446.019
CGAACAA206.310763E-446.035
GCGACTT253.4165947E-546.09
CGAAATT206.310763E-446.022
CATGCGG206.310763E-446.015
ACGCATA253.4165947E-546.012
CTATCGA600.046.034
ACGCAAC206.310763E-446.021
ACCGCTA301.8609753E-646.046
CTATACC301.8609753E-646.010
TCTTACG405.6097633E-946.037
TCGATTG253.4165947E-546.016
GAATACG206.310763E-446.018
CGCGAAA301.8609753E-646.011
TAACGAT206.310763E-446.019
Produced by FastQC (version 0.11.5)