##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527670_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	911250
Sequences flagged as poor quality	0
Sequence length	52
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.213258710562414	33.0	31.0	34.0	31.0	34.0
2	32.69917695473251	34.0	31.0	34.0	31.0	34.0
3	32.750533882030176	34.0	31.0	34.0	31.0	34.0
4	36.08733936899863	37.0	35.0	37.0	35.0	37.0
5	36.27392921810699	37.0	37.0	37.0	35.0	37.0
6	36.02758847736626	37.0	37.0	37.0	35.0	37.0
7	36.51929327846365	37.0	37.0	37.0	35.0	37.0
8	36.587381069958845	37.0	37.0	37.0	35.0	37.0
9	38.5457646090535	39.0	39.0	39.0	37.0	39.0
10	37.79088834019204	39.0	38.0	39.0	35.0	39.0
11	36.37138765432099	38.0	35.0	39.0	33.0	39.0
12	36.144786831275724	38.0	35.0	39.0	33.0	39.0
13	35.875324005486966	38.0	35.0	39.0	32.0	39.0
14	36.686500960219476	39.0	35.0	40.0	33.0	40.0
15	36.803747599451306	39.0	35.0	40.0	33.0	40.0
16	36.783530315500684	39.0	35.0	40.0	33.0	40.0
17	36.70298820301783	39.0	35.0	40.0	32.0	40.0
18	36.68486913580247	39.0	35.0	40.0	32.0	40.0
19	36.46322414266118	38.0	35.0	40.0	31.0	40.0
20	36.483542386831274	38.0	35.0	40.0	31.0	40.0
21	36.53112866941015	38.0	35.0	40.0	31.0	40.0
22	36.42092620027435	38.0	35.0	40.0	31.0	40.0
23	36.438782990397804	38.0	35.0	40.0	31.0	40.0
24	36.517099588477365	38.0	35.0	40.0	32.0	40.0
25	36.45966639231825	38.0	35.0	40.0	32.0	40.0
26	36.21366803840878	38.0	35.0	40.0	31.0	40.0
27	36.05679890260631	38.0	35.0	40.0	31.0	40.0
28	35.91008285322359	37.0	35.0	40.0	31.0	40.0
29	35.79983429355281	37.0	35.0	40.0	31.0	40.0
30	35.506830178326474	37.0	34.0	40.0	30.0	40.0
31	35.384472976680385	36.0	34.0	40.0	30.0	40.0
32	35.253179698216734	36.0	34.0	40.0	30.0	40.0
33	34.90781893004115	35.0	34.0	40.0	28.0	40.0
34	34.644081207133055	35.0	33.0	39.0	27.0	40.0
35	34.63068971193416	35.0	33.0	39.0	27.0	40.0
36	34.647894650205764	35.0	34.0	39.0	28.0	40.0
37	34.61349135802469	35.0	34.0	39.0	29.0	40.0
38	34.31225459533608	35.0	34.0	39.0	28.0	40.0
39	34.31444060356653	35.0	34.0	39.0	28.0	40.0
40	34.18629465020576	35.0	33.0	38.0	27.0	40.0
41	33.83263978052126	35.0	33.0	38.0	26.0	40.0
42	33.89919561042524	35.0	33.0	38.0	27.0	40.0
43	33.86063648834019	35.0	33.0	38.0	27.0	40.0
44	33.736534430727026	35.0	33.0	37.0	27.0	40.0
45	33.60648120713306	35.0	33.0	37.0	26.0	40.0
46	33.237347599451304	35.0	33.0	37.0	24.0	40.0
47	33.05471165980796	35.0	33.0	36.0	23.0	40.0
48	33.08059478737997	35.0	33.0	36.0	24.0	40.0
49	33.033967626886145	35.0	33.0	36.0	25.0	39.0
50	32.903624691358026	35.0	32.0	36.0	24.0	39.0
51	33.24414814814815	35.0	33.0	36.0	26.0	39.0
52	32.95917914951989	35.0	33.0	35.0	26.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	8.0
15	12.0
16	50.0
17	163.0
18	303.0
19	576.0
20	1019.0
21	1695.0
22	2549.0
23	3758.0
24	5449.0
25	7355.0
26	9351.0
27	12350.0
28	14715.0
29	18508.0
30	22933.0
31	29156.0
32	39576.0
33	63028.0
34	133104.0
35	104794.0
36	112267.0
37	138212.0
38	151078.0
39	39241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.3512208504801	0.5586831275720165	0.04279835390946502	0.04729766803840878
8	99.519890260631	0.3573113854595336	0.047956104252400544	0.07484224965706447
9	95.37108367626887	2.213772290809328	1.2216186556927298	1.193525377229081
10	54.23659807956104	31.47862825788752	5.4736899862825785	8.811083676268861
11	43.13700960219479	20.214540466392318	18.9682304526749	17.680219478737996
12	37.1838683127572	14.01437585733882	24.149245541838134	24.652510288065844
13	27.306776406035667	15.011796982167352	26.69443072702332	30.98699588477366
14	25.583429355281208	19.041865569272975	30.01349794238683	25.361207133058983
15	32.54211248285323	17.514842249657065	27.523950617283948	22.41909465020576
16	35.669574759945135	18.927187928669408	24.546941015089164	20.856296296296296
17	33.68735253772291	20.398134430727026	23.631385459533607	22.28312757201646
18	33.5836488340192	20.67533607681756	22.44290809327846	23.298106995884773
19	29.94063100137174	24.777283950617285	22.479890260631002	22.802194787379975
20	33.40905349794239	24.531687242798352	21.2081207133059	20.85113854595336
21	30.002962962962965	23.538106995884775	24.942880658436213	21.516049382716048
22	28.238792866941015	21.02310013717421	27.14085048010974	23.597256515775033
23	30.869355281207135	21.353305898491083	25.487626886145403	22.28971193415638
24	32.922139917695475	21.290096021947875	21.041207133058982	24.746556927297668
25	28.481755829903975	21.083676268861453	21.043731138545954	29.390836762688615
26	27.406310013717423	19.825185185185184	24.809218106995885	27.95928669410151
27	25.273854595336076	19.524609053497944	23.441536351165983	31.759999999999998
28	25.805212620027433	25.64993141289438	20.61355281207133	27.93130315500686
29	31.85514403292181	22.469903978052127	21.57903978052126	24.095912208504803
30	28.06562414266118	20.936186556927296	24.929931412894376	26.06825788751715
31	33.60285322359397	19.755720164609052	23.402798353909464	23.238628257887516
32	36.24329218106996	18.86244170096022	22.98897119341564	21.905294924554184
33	33.86271604938272	19.54008230452675	25.10606310013717	21.491138545953362
34	27.994732510288067	22.26875171467764	29.15116598079561	20.585349794238684
35	23.856131687242797	28.513141289437588	28.018875171467766	19.611851851851853
36	27.07500685871056	30.05816186556927	21.90650205761317	20.960329218106995
37	31.130644718792865	27.141289437585737	21.11769547325103	20.610370370370372
38	30.160438957475993	28.87133058984911	19.668257887517147	21.299972565157752
39	30.72	24.4402743484225	19.85832647462277	24.98139917695473
40	28.248340192043898	22.182057613168723	21.12866941015089	28.440932784636487
41	23.64400548696845	26.86255144032922	20.170205761316872	29.32323731138546
42	23.637091906721537	23.429026063100135	20.75017832647462	32.183703703703706
43	24.456625514403292	20.842469135802467	22.95736625514403	31.74353909465021
44	25.811248285322357	21.57212620027435	23.234787379972566	29.381838134430726
45	27.749245541838135	22.30244170096022	22.191275720164608	27.757037037037037
46	27.15149519890261	25.19835390946502	21.762633744855968	25.887517146776407
47	26.16164609053498	22.177009602194786	24.661179698216735	27.000164609053495
48	27.54216735253772	20.357091906721536	26.682139917695473	25.418600823045267
49	28.975363511659808	20.671714677640605	24.54068587105624	25.812235939643347
50	24.444334705075445	20.896241426611798	26.224526748971194	28.434897119341564
51	24.607736625514402	20.833251028806586	27.126255144032925	27.43275720164609
52	23.908367626886147	21.547764060356652	26.630013717421125	27.91385459533608
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.5
10	3.0
11	2.5
12	2.0
13	20.5
14	216.5
15	394.0
16	521.0
17	648.0
18	1005.0
19	1362.0
20	1415.0
21	1468.0
22	1277.5
23	1087.0
24	1510.0
25	1933.0
26	1849.0
27	1765.0
28	1849.0
29	1933.0
30	2922.5
31	3912.0
32	3745.0
33	3578.0
34	4395.0
35	5212.0
36	6101.0
37	6990.0
38	7520.0
39	9236.5
40	10423.0
41	12516.5
42	14610.0
43	15448.0
44	16286.0
45	18918.5
46	21551.0
47	21631.5
48	21712.0
49	24195.5
50	26679.0
51	29043.0
52	31407.0
53	35656.0
54	39905.0
55	41231.0
56	42557.0
57	47813.0
58	53069.0
59	60570.5
60	68072.0
61	73722.0
62	79372.0
63	85082.0
64	90977.0
65	91162.0
66	89463.0
67	87764.0
68	71284.0
69	54804.0
70	49625.0
71	44446.0
72	34831.0
73	25216.0
74	21726.5
75	18237.0
76	16206.5
77	14176.0
78	12149.0
79	10122.0
80	8376.5
81	6631.0
82	4505.5
83	2380.0
84	1638.0
85	896.0
86	706.5
87	517.0
88	289.5
89	39.0
90	16.0
91	12.0
92	8.0
93	4.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	911250.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.793801450754644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.01562558715995	9.59735019466274
2	9.623863658736825	2.462516016780242
3	3.4175344897463176	1.3116977313876141
4	1.5812669970036295	0.809216640011819
5	0.8695755773449215	0.5562588641488132
6	0.5212974472425849	0.40016256220841245
7	0.3540589327408993	0.31708317811472103
8	0.25338487034710055	0.25934045774768094
9	0.19707684189225366	0.2269225787140133
>10	3.7282489794298503	13.535125061710879
>50	2.8537740942713947	25.941422515778534
>100	1.5109219494632704	33.531156155286844
>500	0.047776188125320806	4.144200860355957
>1k	0.023888094062660403	4.568575411163582
>5k	8.531462165235859E-4	0.7500776525591586
>10k+	8.531462165235859E-4	1.588894119368986
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	14557	1.5974759945130317	No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	6872	0.7541289437585734	No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	3359	0.3686145404663923	No Hit
CCGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2619	0.2874074074074074	No Hit
CCGACGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	2313	0.2538271604938272	No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGTGTAAGAGCACCCAACTGCT	2082	0.22847736625514403	No Hit
CCGACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	2049	0.2248559670781893	No Hit
CCGACGGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTC	1716	0.18831275720164609	No Hit
CCGACGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1654	0.18150891632373115	No Hit
CCGACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1552	0.17031550068587104	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1498	0.16438957475994515	No Hit
CCGACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1451	0.1592318244170096	No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	1397	0.15330589849108367	No Hit
CCGACGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCGACGGGGAAG	1395	0.1530864197530864	No Hit
CCGACGGGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1318	0.14463648834019205	No Hit
CCGACGGGGGGGCTGGAGAGATGGCTTAGCGGTAAAGAGCAATGACTGCTCT	1297	0.14233196159122086	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1293	0.14189300411522635	No Hit
CCGACGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCTCCGACTGCTCT	1261	0.13838134430727023	No Hit
CCGACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT	1257	0.13794238683127572	No Hit
CCGACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1254	0.13761316872427984	No Hit
CCGACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTT	1231	0.13508916323731138	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT	1205	0.13223593964334704	No Hit
CCGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1191	0.13069958847736626	No Hit
CCGACGGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGCTC	1178	0.1292729766803841	No Hit
CCGACGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	1129	0.12389574759945131	No Hit
CCGACGGGGGGGGGCTGGCGAGATGGCTCAGTGGATAAGAGCACTGACTGCT	1042	0.11434842249657064	No Hit
CCGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC	1034	0.11347050754458163	No Hit
CCGACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT	1029	0.11292181069958848	No Hit
CCGACGGGGAGCCGGGCGGTGGTGGCACACGCCTTTAATCCCAGCACTTGGG	1029	0.11292181069958848	No Hit
CCGACGGGGGGGCCTCAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	1023	0.11226337448559671	No Hit
CCGACGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	981	0.10765432098765432	No Hit
CCGACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	979	0.10743484224965706	No Hit
CCGACGGGGGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGCCAGTGAATCT	960	0.10534979423868314	No Hit
CCGACGGGGGGCTGGGAGGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGG	949	0.10414266117969823	No Hit
CCGACGGGGGGAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCAGACTG	935	0.10260631001371742	No Hit
CCGACGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTC	915	0.10041152263374487	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	8.779149519890261E-4	0.0	0.0	0.0	0.0
8	0.0016460905349794238	0.0	0.0	0.0	0.0
9	0.008010973936899863	0.0	0.0	0.0	0.0
10	0.024362139917695473	0.0	0.0	0.0	0.0
11	0.042578875171467766	0.0	0.0	0.0	0.0
12	0.05212620027434842	0.0	0.0	0.0	0.0015363511659807956
13	0.05936899862825789	0.0	0.0	0.0	0.0015363511659807956
14	0.08669410150891632	0.0	0.0	0.0	0.0015363511659807956
15	0.10315500685871057	0.0	0.0	0.0	0.0015363511659807956
16	0.11862825788751714	0.0	0.0	0.0	0.0015363511659807956
17	0.13256515775034294	0.0	0.0	0.0	0.0015363511659807956
18	0.1436488340192044	0.0	0.0	0.0	0.0015363511659807956
19	0.15835390946502056	0.0	0.0	0.0	0.0015363511659807956
20	0.17020576131687243	0.0	0.0	0.0	0.0015363511659807956
21	0.18128943758573388	0.0	0.0	0.0	0.0015363511659807956
22	0.19204389574759945	0.0	0.0	0.0	0.0015363511659807956
23	0.20521262002743484	0.0	0.0	0.0	0.0015363511659807956
24	0.2213443072702332	0.0	0.0	0.0	0.0015363511659807956
25	0.23231824417009603	0.0	0.0	0.0	0.0015363511659807956
26	0.25689986282578875	0.0	0.0	0.0	0.0015363511659807956
27	0.27083676268861456	0.0	0.0	0.0	0.0015363511659807956
28	0.3145130315500686	0.0	0.0	0.0	0.0015363511659807956
29	0.32603566529492456	0.0	0.0	0.0	0.0015363511659807956
30	0.3519341563786008	0.0	0.0	0.0	0.0015363511659807956
31	0.361920438957476	0.0	0.0	0.0	0.0015363511659807956
32	0.379039780521262	0.0	0.0	0.0	0.0015363511659807956
33	0.39122085048010974	0.0	0.0	0.0	0.0015363511659807956
34	0.4071330589849108	0.0	0.0	0.0	0.0015363511659807956
35	0.45037037037037037	0.0	0.0	0.0	0.0015363511659807956
36	0.4656241426611797	0.0	0.0	0.0	0.0015363511659807956
37	0.48263374485596705	0.0	0.0	0.0	0.0015363511659807956
38	0.4975582990397805	0.0	0.0	0.0	0.0015363511659807956
39	0.5085322359396434	0.0	0.0	0.0	0.0015363511659807956
40	0.5202743484224965	0.0	0.0	0.0	0.0015363511659807956
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCATA	35	1.0192525E-7	46.000004	31
CTTCGTA	20	6.310763E-4	46.0	14
AGGTATG	20	6.310763E-4	46.0	41
AGCGTAC	50	1.6370905E-11	46.0	12
AACCGGT	20	6.310763E-4	46.0	34
ACCGGTA	20	6.310763E-4	46.0	19
CGAACAA	20	6.310763E-4	46.0	35
GCGACTT	25	3.4165947E-5	46.0	9
CGAAATT	20	6.310763E-4	46.0	22
CATGCGG	20	6.310763E-4	46.0	15
ACGCATA	25	3.4165947E-5	46.0	12
CTATCGA	60	0.0	46.0	34
ACGCAAC	20	6.310763E-4	46.0	21
ACCGCTA	30	1.8609753E-6	46.0	46
CTATACC	30	1.8609753E-6	46.0	10
TCTTACG	40	5.6097633E-9	46.0	37
TCGATTG	25	3.4165947E-5	46.0	16
GAATACG	20	6.310763E-4	46.0	18
CGCGAAA	30	1.8609753E-6	46.0	11
TAACGAT	20	6.310763E-4	46.0	19
>>END_MODULE