FastQCFastQC Report
Fri 17 Jun 2016
SRR1527669_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527669_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100937
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGA15501.535611321913669No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC7310.7242141137541238No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6970.6905297363702111No Hit
GATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6570.6509010570950197No Hit
GATCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3100.3071222643827338No Hit
GATCTTGGGAGGCCTCTGTGGCTACCAACCCCATCATCACCCCAGTGGCC2750.27244717001694124No Hit
GATCTTGGGGGCCCAGTTGATAAAGTGCTTGCTCTGGAAGCATGACCCAT2490.2466885284880668No Hit
GATCTTGGGGGAGATGTGAGCATTTGCCAAGCTATGTATTCATCTGGATT2470.24470709452430725No Hit
GATCTTGGGACCAGGAGACAGTAAAGGAGCTGCTGCAGGCAGGTTCTGCA2440.2417349435786679No Hit
GATCTTGGGAGATGATGCATATATTGATATCTTTTATTGCAAAAATGTAA2340.23182777375987002No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAA2330.2308370567779902No Hit
GATCTTGGGGCTAGGCACCCACACACCCATGTCCAACTCCTTTCTTTATC2320.22984633979611044No Hit
GATCTTGGGTGCTGATCTTGGGAAAAGAAACTGTAAAAAGATCAGGGATA2310.22885562281423064No Hit
GATCTTGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAA2300.22786490583235086No Hit
GATCTTGGGAGAAATTCTGAGTATACTTGATTGTATCTTAAAAGTTAAAA2250.22291132092295196No Hit
GATCTTGGGATGGCTGTTAGCATAGAGTTTAAAAAAAGAGAGATAAGCTA2240.22192060394107216No Hit
GATCTTGGGGTGTTATATGTGTTTACATTAAGATCTGAATTATTTAAAAT2230.22092988695919238No Hit
GATCTTGGGACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGA2220.21993916997731258No Hit
GATCTTGGGGCCCTGCGCCTAGTTACAGTTCCACTTCCTTTGATGCTCCG2180.21597630204979343No Hit
GATCTTGGGTGGCTTTGTGGCTAATGGGGAAAAATAACTATTTTAAAAGG2180.21597630204979343No Hit
GATCTTGGGAGAGTGCAGTGCTGATCTTGGGAACCAGCTGTATCATTTGC2150.21300415110415408No Hit
GATCTTGGGAATCAAGGTTAAAATATCACTAGCAATAAGACACATCAACA2140.21201343412227427No Hit
GATCTTGGGTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGC2130.2110227171403945No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGCAATATCAGAAGTTCCCTCT2080.20606913223099554No Hit
GATCTTGGGAGCCCCTACACCAAGTATGCTAGCATGATCAACAAGGCCAC2080.20606913223099554No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAA2050.20309698128535622No Hit
GATCTTGGGGAATGCCCTTCAGCCTAGAGCGCGGAGGAGGACGGTAGCTC2030.20111554732159664No Hit
GATCTTGGGATTGCAGGCTGATTCCTCCAGGAATATAATAGTGACGTTGA2030.20111554732159664No Hit
GATCTTGGGGCCTCTGCCTCTGCCTCTGCCTCTGCCTCTGCCTCTGCCTC2020.20012483033971687No Hit
GATCTTGGGCAGGCAGTGGTGGCACACACCTTTGATCCCAGCACTTGGGA1960.19418052844843814No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGGAGCCAGGCAGTGGTGGCGCAT1950.19318981146655834No Hit
GATCTTGGGACACTAATTACTGTTTGTAGTGTCTTTGGATGCTCACGGGT1950.19318981146655834No Hit
GATCTTGGGATCTGCCCCCTCCCCCCCTCACAGCCAGCAGCATTTCCAAG1910.18922694353903918No Hit
GATCTTGGGAGCATTCTTTTGGACACCACACCTAGGTCGGAGCACTACCG1870.18526407561152006No Hit
GATCTTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA1870.18526407561152006Illumina Single End Adapter 2 (100% over 34bp)
GATCTTGGGAGCTGGTCTTTGAATCTGCATGTACTTCACGTTTTCTATAT1860.18427335862964028No Hit
GATCTTGGGGAATTTGACTGATGCCAGGCGTGGTGGTGCACACCTTTAAT1860.18427335862964028No Hit
GATCTTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA1840.1822919246658807No Hit
GATCTTGGGGGAGCGCAGGGAACTGCACACAATACCTCCTAATAACCTAC1790.17733833975648175No Hit
GATCTTGGGGATCTACAGTCACTTCAAACAAGCTACTGTGGGCGATGTAA1790.17733833975648175No Hit
GATCTTGGGGTTCGCAGTAAGCATTTGCACGGTTACTTAGTGAAACTTTT1780.17634762277460198No Hit
GATCTTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCT1750.17337547182896262No Hit
GATCTTGGGACGATACATACTATGTAGTAGCCCATTTCCACTATGTTCTA1730.17139403786520305No Hit
GATCTTGGGAGCTGCTGACCTCTGCCAGGGCAGGTACCACTTCCTTGGGA1680.16644045295580412No Hit
GATCTTGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.16644045295580412No Hit
GATCTTGGGGGGAGATGGAGGTGAGCCAGGCGGCGTCTGGTACCGACGGT1660.16445901899204454No Hit
GATCTTGGGGTGCTTCTTTTGCAAGCCCTGCCTGAGACCCAAATCAACAG1660.16445901899204454No Hit
GATCTTGGGTACCCTAAAAAGGGTACCCAAATGAGGCGCTGCACTTAGTA1650.16346830201016477No Hit
GATCTTGGGGCAGCGGGCAACCTGCCAAGCCTTTAGCTTTCTCTGGTCTA1640.16247758502828497No Hit
GATCTTGGGAGAAGGGGTCGGGGAGCATCAGGACTTGGGTTTTCTATGCT1620.1604961510645254No Hit
GATCTTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1620.1604961510645254No Hit
GATCTTGGCGCAGAGTGCAGTGCTGATCTTGGGGGGTGGCTGGAGAGATG1620.1604961510645254No Hit
GATCTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC1600.1585147171007658No Hit
GATCTTGGGAGGTGAGGGCTTTGGGAAGCCCTCCAGATAACTCTTGTTGC1600.1585147171007658No Hit
GATCTTGGGTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGTGCCAT1580.15653328313700623No Hit
GATCTTGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGC1560.15455184917324669No Hit
GATCTTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTGAGAGCACTGACTG1550.1535611321913669No Hit
GATCTTGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAA1540.1525704152094871No Hit
GATCTTGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGT1540.1525704152094871No Hit
GATCTTGGGGGTCTGTTCTGCACCAACTCAGAGTCTTGTTGGAGCTTTAT1520.15058898124572753No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG1520.15058898124572753No Hit
GATCTTGGGATCTGCTCTCTGGTGATGTCTGCCTGTGAACGCACCTTATA1520.15058898124572753No Hit
GATCTTGGGGCCTTTTCCGGTCGCGGCGCTGCGCGGTGTGGAGCTCGTGC1510.14959826426384776No Hit
GATCTTGGGGCTTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTAC1510.14959826426384776No Hit
GATCTTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT1500.14860754728196796No Hit
GATCTTGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGT1480.14662611331820838No Hit
GATCTTAGGGATTGATTTGATAGACATGTTGAGTTACTACTTTGCTGTAC1470.1456353963363286No Hit
GATCTTGGGGCTTCTTAAACCTGAAGTGGAGTACTACTCTGGTTGCGCTT1460.14464467935444883No Hit
GATCTTGGGTATGGTGCATTTTTTTCGCTATTTAAGTTGGATATTTCTCT1450.14365396237256903No Hit
GATCTTGGGCAGAGTGCAGTGCTGATCTTGGGCACCCCAAAAGACGTAGA1450.14365396237256903No Hit
GATCTTGGGATGAGGGCCAAGCCCAGGTTGCTTCTATTTGCTTCACCTTT1420.14068181142692968No Hit
GATCTTGGGTGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGG1380.13671894349941052No Hit
GATCTTGGGGAAAGGAATCCCAAGAAAGCCAAAAGTGGTGCTACCTTTCC1370.13572822651753072No Hit
GATCTTGGGATTATCTCAAGACTTCCTCAAAAAAGAATTGGAGAACAATG1360.13473750953565095No Hit
GATCTTGGGCGTAGTTCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGC1360.13473750953565095No Hit
GATCTTGGGGTTTAGTTTCAAGCGGACCGCGCCGGCTTCAGGCTATTGGA1350.13374679255377117No Hit
GATCTTGGGATCGCCAGTGGGCAAACCTGATGGAGAAGATACAGGCCTCT1330.1317653585900116No Hit
GATCTTGGGGCAAAGGGTTTTTGTGAATTACGGTGGTGGGTAAGACCCGA1330.1317653585900116No Hit
GATCTTGGGAGCCCATATCTGTGGAATGGAATATAAATATCACAAAGCCA1330.1317653585900116No Hit
GATCTTGGGATGGCCGTACACCCACCCTCTAAGGCCACCAGTTGTCTCTA1310.12978392462625202No Hit
GATCTTGGGTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTGT1310.12978392462625202No Hit
GATCTTGGGGGGCTGTGCTGTGTGTGCGACCGATGCCCTGCGGCTCAAGT1310.12978392462625202No Hit
GATCTTGGGCTTCTTAATGAAGAAAGCAGAATCAGACACCTAAGACCAAT1300.12879320764437224No Hit
GATCTTGGGCAAATTGTGTGACACATATTCATGCATAAACTGTTAAAACG1300.12879320764437224No Hit
GATCTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA1290.12780249066249244No Hit
GATCTTGGGGAGAGACCCGGAGTCCGAATTCAGAGGCCCCAGTCTGTAGT1280.12681177368061267No Hit
GATCTTGGGACTTCCACGGAGTCTCAAACCTGCAGGAATTTCGTTTCATG1270.12582105669873286No Hit
GATCTTGGCGCAGGGCCTTTGTTCTACCTCTGAGGACAGAGAGCAGTTTC1270.12582105669873286No Hit
GATCTTGGGAGAGTGCAGTCCTGATCTTGGGTTCTGAAATTACTTTTGGG1260.12483033971685308No Hit
GATCTTGGGGAGAAGCGGCTCATTCCTGATGGCTGTGGTGTCAAATATAT1250.12383962273497329No Hit
GATCTTGGGTGCTGATCTTGGGGTCACGAAATCCTTCGCTTAATTGCCGC1250.12383962273497329No Hit
GATCTTGGAAGGAGGAAGAAGAATAGTCCTGAAGCAGGTGTGGAGAGGTC1240.12284890575309353No Hit
GATCTTGGGGAAGACATAGAAGACCTGGAACCCAGGGATGCCCAGTCCCA1240.12284890575309353No Hit
GATCTTGGGTAACCTGCCGTCCGGTAGCCTGCTGCCTTTGTGGCTTTTTA1240.12284890575309353No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGG1240.12284890575309353No Hit
GATCTTGGGCTGCTTTGCTTTCCCTTTCCACCTCAAGACTTCAAGAAGAC1230.12185818877121374No Hit
GATCTTGGGGAATTCTATGTTTTTAAAATTTTTTAATTTAAAAAAGTAAA1230.12185818877121374No Hit
GATCTTGGGGGCCAACAGCTCCACGGGGTGGTCTGGCCCCACTGCGCGCT1230.12185818877121374No Hit
GATCTTGGGGTGCTGATCTTGGGGGGCAGTTGCTGGTACTTGGCCCTCAG1220.12086747178933395No Hit
GATCTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG1220.12086747178933395No Hit
GATCTTGGGGCACCTACCACATAAATTATAATGTTTAATGTTTTGAAAAT1210.11987675480745416No Hit
GATCTTGGGTCATAAACTAAACAATTACCTACCACCTCCATCTCTTCCCC1200.11888603782557437No Hit
GATCTTGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGA1200.11888603782557437No Hit
GATCTTGGGTAGTTGTGTTGTTTACAATACTCTGGAAGTAAACTGCTGCC1200.11888603782557437No Hit
GATCTTGGGATCAGCCAGTAAGATAATGTGCTTTTCTGGTTCGCTCAATA1190.11789532084369458No Hit
GATCTTGGGAAATTACACAACCTAACTCCCCCCAAAGAATCTGTATAAAG1180.1169046038618148No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGATTGACAGTATTGTAAACAGTC1180.1169046038618148No Hit
GATCTTGTTGGAGGATTGGATATTGTCAAGGAACTGAAAGACAACGGTGA1170.11591388687993501No Hit
GATCTTGGGATCCGTGAGCGCCTGGGGCCTCTGGCGTCCCAGCCTCTGCG1160.11492316989805522No Hit
GATCTTGGGTGGGGCAGTCCTTGCTTCCTGCTTGCTATCCCACAGGAATC1160.11492316989805522No Hit
GATCTTGGGATCTGATGTTACCATTGCGATCTAGCTTTCTTTTCATTAGC1150.11393245291617543No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG1140.11294173593429564Illumina Single End Adapter 2 (100% over 23bp)
GATCTTGGGGCCTCTTGAGTTCTAGGTTCACCTGATCTGCAGAATGCTTT1140.11294173593429564No Hit
GATCTTGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGT1130.11195101895241587No Hit
GATCTTGGGCTCAGCAGTTCTCGGTCATTTGATTTTGCATTTTGCACTTC1130.11195101895241587No Hit
GATCTTGGGTATCGAGTGCAGCCCTGATCTTGGGAAGTCCTCTAATATGG1120.11096030197053608No Hit
GATCTTGGGGGCTCCTTCCCCAGGAGATTCATCTCCAAGAGAAGGAGCCG1110.10996958498865629No Hit
GATCTTGGGTACGGGAACTTGCTTCCCTCTCCTGCCCCTCCTGCTGACTG1100.1089788680067765No Hit
GATCTTGGGTACAGAGCAATGCTACATTGTTGTAAAAAGAATTGCTATTG1100.1089788680067765No Hit
GATCTTGGGGCGGCTAGAATCTGCCAACGAGTCGCGTAGTAGCCCTATCG1090.10798815102489671No Hit
GATCTTGGGATCCTTCTCCTGTCCTGCAACAACATCCATATCCAGCCAGG1090.10798815102489671No Hit
GATCTTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG1080.10699743404301693No Hit
GATCTTGGGAGATCCTGGCCGCTGCAGCCCGCTCAGGCAGGGGACAGCGT1060.10501600007925735No Hit
GATCTTGGGGTGCTGATCTTGGGGAGCATCTGGGACGTGAAGAGCCTGGA1060.10501600007925735No Hit
GATCTTGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCAC1060.10501600007925735No Hit
GATCTTGGGCAGCTCTCCCGGCAGACTAACCTCATAGTGGTCAAGTACTC1050.10402528309737756No Hit
GATCTTGGGAACGCAGAGTGCAGTGCTGATCTTGGGGAGAAGGAGAGAGA1050.10402528309737756No Hit
GATCTTGGGGCTTTTAAAGGCGCTAGCTGGTGCGCTCTGTGACTTGATTG1040.10303456611549777No Hit
GATCTTGGGGCGGATGCCCCAGCTGCTCGCTACCAGACAATTGAAGAGAA1020.10105313215173822No Hit
GATCTTGGGGATGGTCGGTGTGCGCGACCGTGGCCCGGTTGACTGAGCAG1010.10006241516985843No Hit
GATCTTGGGCATATGGCACATCCCTGTAATCCCACCGCCTGGAAGGCAGC1010.10006241516985843No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGTA207.822344E-444.00000433
GCCCTAA207.822344E-444.00000414
CGCGACT207.822344E-444.00000416
ACAGCGT207.822344E-444.00000444
ACAAATG207.822344E-444.00000426
CGCTGCG207.822344E-444.00000427
ACAGTCA302.504743E-644.00000415
ACAGTAA207.822344E-444.00000418
TTTGAAT207.822344E-444.00000418
AAGTATG207.822344E-444.00000422
TAGCGAT207.822344E-444.00000442
TTGTCCA207.822344E-444.00000444
ACTGTAG207.822344E-444.00000412
ACACGGT207.822344E-444.00000432
GAGAAGC207.822344E-444.00000410
TTCACGT207.822344E-444.00000435
CAATGGG207.822344E-444.00000438
GGTGAGC207.822344E-444.00000420
GAGAAAG207.822344E-444.00000442
GAGCGCG302.504743E-644.00000427
Produced by FastQC (version 0.11.5)