FastQCFastQC Report
Fri 17 Jun 2016
SRR1527668_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527668_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149082
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGAAG17521.1751921761178412No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC10800.7244335332233268No Hit
GATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8730.5855837726888558No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8400.563448303618143No Hit
GATCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4200.2817241518090715No Hit
GATCTTGGGGGCCCAGTTGATAAAGTGCTTGCTCTGGAAGCATGACCCATCC4170.2797118364390067No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAA3930.2636133134784884No Hit
GATCTTGGGTGGCTTTGTGGCTAATGGGGAAAAATAACTATTTTAAAAGGTA3870.2595886827383587No Hit
GATCTTGGGAGATGATGCATATATTGATATCTTTTATTGCAAAAATGTAAAT3710.24885633409801317No Hit
GATCTTGGGGGAGATGTGAGCATTTGCCAAGCTATGTATTCATCTGGATTGT3530.23678244187762437No Hit
GATCTTGGGCAGGCAGTGGTGGCACACACCTTTGATCCCAGCACTTGGGAGG3520.2361116700876028No Hit
GATCTTGGGGTGTTATATGTGTTTACATTAAGATCTGAATTATTTAAAATTT3420.22940395218738682No Hit
GATCTTGGGATTGCAGGCTGATTCCTCCAGGAATATAATAGTGACGTTGAGT3360.2253793214472572No Hit
GATCTTGGGACCAGGAGACAGTAAAGGAGCTGCTGCAGGCAGGTTCTGCACA3330.2233670060771924No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGCAATATCAGAAGTTCCCTCTTG3260.2186716035470412No Hit
GATCTTGGGATGGCTGTTAGCATAGAGTTTAAAAAAAGAGAGATAAGCTAAT3230.2166592881769764No Hit
GATCTTGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAA3230.2166592881769764No Hit
GATCTTGGGAGCCCCTACACCAAGTATGCTAGCATGATCAACAAGGCCACAC3220.21598851638695485No Hit
GATCTTGGGACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG3190.21397620101689002No Hit
GATCTTGGGAATCAAGGTTAAAATATCACTAGCAATAAGACACATCAACACC3170.21263465743684684No Hit
GATCTTGGGATCTGCCCCCTCCCCCCCTCACAGCCAGCAGCATTTCCAAGTG3140.21062234206678204No Hit
GATCTTGGGAGAAATTCTGAGTATACTTGATTGTATCTTAAAAGTTAAAACT3110.20861002669671724No Hit
GATCTTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA3100.20793925490669565No Hit
GATCTTGGGTGCTGATCTTGGGAAAAGAAACTGTAAAAAGATCAGGGATAAC3020.20257308058652285No Hit
GATCTTGGGGATCTACAGTCACTTCAAACAAGCTACTGTGGGCGATGTAAAT2980.19988999342643643No Hit
GATCTTGGGGTTCGCAGTAAGCATTTGCACGGTTACTTAGTGAAACTTTTCT2980.19988999342643643No Hit
GATCTTGGGGCCCTGCGCCTAGTTACAGTTCCACTTCCTTTGATGCTCCGTA2980.19988999342643643No Hit
GATCTTGGGGCTAGGCACCCACACACCCATGTCCAACTCCTTTCTTTATCCA2970.19921922163641487No Hit
GATCTTGGGTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGA2960.19854844984639325No Hit
GATCTTGGGAGAGTGCAGTGCTGATCTTGGGAACCAGCTGTATCATTTGCCT2920.19586536268630686No Hit
GATCTTGGGACACTAATTACTGTTTGTAGTGTCTTTGGATGCTCACGGGTTC2890.19385304731624206No Hit
GATCTTGGGAGCTGGTCTTTGAATCTGCATGTACTTCACGTTTTCTATATTT2850.19116996015615567No Hit
GATCTTGGGGCCTCTGCCTCTGCCTCTGCCTCTGCCTCTGCCTCTGCCTCTG2840.19049918836613408No Hit
GATCTTGGGGAATGCCCTTCAGCCTAGAGCGCGGAGGAGGACGGTAGCTCGG2810.18848687299606928No Hit
GATCTTGGGAACGCAGAGTGCAGTGCTGATCTTGGGGAGAAGGAGAGAGAGA2800.18781610120604766No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT2780.18647455762600448No Hit
GATCTTGGGGAATTTGACTGATGCCAGGCGTGGTGGTGCACACCTTTAATCC2760.1851330140459613No Hit
GATCTTGGGGGGAGATGGAGGTGAGCCAGGCGGCGTCTGGTACCGACGGTGT2740.18379147046591807No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAA2740.18379147046591807No Hit
GATCTTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC2700.1811083833058317No Hit
GATCTTGGGATCTGCTCTCTGGTGATGTCTGCCTGTGAACGCACCTTATAAA2700.1811083833058317No Hit
GATCTTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTGAGAGCACTGACTGCT2700.1811083833058317No Hit
GATCTTGGGCAGAGTGCAGTGCTGATCTTGGGCACCCCAAAAGACGTAGAAA2680.1797668397257885No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGGAGCCAGGCAGTGGTGGCGCATGC2670.1790960679357669No Hit
GATCTTGGGAGGCCTCTGTGGCTACCAACCCCATCATCACCCCAGTGGCCCA2600.17440066540561572No Hit
GATCTTGGGAGCATTCTTTTGGACACCACACCTAGGTCGGAGCACTACCGTC2560.1717175782455293No Hit
GATCTTGGGGTGCTTCTTTTGCAAGCCCTGCCTGAGACCCAAATCAACAGCT2550.1710468064555077No Hit
GATCTTGGGAGCTGCTGACCTCTGCCAGGGCAGGTACCACTTCCTTGGGACC2540.1703760346654861No Hit
GATCTTGGGGGAGCGCAGGGAACTGCACACAATACCTCCTAATAACCTACCT2510.16836371929542132No Hit
GATCTTGGGAGAAGGGGTCGGGGAGCATCAGGACTTGGGTTTTCTATGCTGT2500.16769294750539973No Hit
GATCTTGGGTATGGTGCATTTTTTTCGCTATTTAAGTTGGATATTTCTCTAC2490.1670221757153781No Hit
GATCTTGGGTACCCTAAAAAGGGTACCCAAATGAGGCGCTGCACTTAGTATA2450.16433908855529172No Hit
GATCTTGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG2450.16433908855529172No Hit
GATCTTGGGTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGTGCCATGA2440.16366831676527013No Hit
GATCTTGGGGCAGCGGGCAACCTGCCAAGCCTTTAGCTTTCTCTGGTCTATA2440.16366831676527013No Hit
GATCTTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC2430.16299754497524852No Hit
GATCTTGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT2420.16232677318522692No Hit
GATCTTGGGACGATACATACTATGTAGTAGCCCATTTCCACTATGTTCTATC2420.16232677318522692No Hit
GATCTTGGCGCAGAGTGCAGTGCTGATCTTGGGGGGTGGCTGGAGAGATGGC2390.16031445781516213No Hit
GATCTTGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2370.15897291423511892No Hit
GATCTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG2360.15830214244509733No Hit
GATCTTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2360.15830214244509733Illumina Single End Adapter 2 (100% over 34bp)
GATCTTGGGGCTTCTTAAACCTGAAGTGGAGTACTACTCTGGTTGCGCTTGT2350.15763137065507574No Hit
GATCTTGGGTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTGTCC2260.15159442454488134No Hit
GATCTTGGGGTGCTGATCTTGGGGGGCAGTTGCTGGTACTTGGCCCTCAGCC2250.15092365275485975No Hit
GATCTTGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAAT2240.15025288096483816No Hit
GATCTTGGGAGGTGAGGGCTTTGGGAAGCCCTCCAGATAACTCTTGTTGCTC2190.14689902201473015No Hit
GATCTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC2170.14555747843468694No Hit
GATCTTGGGGCCTTTTCCGGTCGCGGCGCTGCGCGGTGTGGAGCTCGTGCTT2160.14488670664466535No Hit
GATCTTGGGGAGAAGCGGCTCATTCCTGATGGCTGTGGTGTCAAATATATCC2140.14354516306462214No Hit
GATCTTGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTG2100.14086207590453576No Hit
GATCTTGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTT2100.14086207590453576No Hit
GATCTTGGGATGAGGGCCAAGCCCAGGTTGCTTCTATTTGCTTCACCTTTAA2100.14086207590453576No Hit
GATCTTGGGCTGCTTTGCTTTCCCTTTCCACCTCAAGACTTCAAGAAGACAG2090.14019130411451416No Hit
GATCTTGGGGGTCTGTTCTGCACCAACTCAGAGTCTTGTTGGAGCTTTATCC2080.13952053232449257No Hit
GATCTTGGGATGGCCGTACACCCACCCTCTAAGGCCACCAGTTGTCTCTAGA2070.13884976053447098No Hit
GATCTTGGGTGGGGCAGTCCTTGCTTCCTGCTTGCTATCCCACAGGAATCTG2070.13884976053447098No Hit
GATCTTGGGGAAAGGAATCCCAAGAAAGCCAAAAGTGGTGCTACCTTTCCTC2060.13817898874444937No Hit
GATCTTGGCGCAGGGCCTTTGTTCTACCTCTGAGGACAGAGAGCAGTTTCCC2060.13817898874444937No Hit
GATCTTGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTG2040.13683744516440616No Hit
GATCTTGGGGCTTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACAC1990.13348358621429818No Hit
GATCTTGGGCTTCTTAATGAAGAAAGCAGAATCAGACACCTAAGACCAATCA1980.1328128144242766No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGATTGACAGTATTGTAAACAGTCTG1950.13080049905421176No Hit
GATCTTGGGCGTAGTTCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCG1940.13012972726419017No Hit
GATCTTGGGCAAATTGTGTGACACATATTCATGCATAAACTGTTAAAACGAG1910.12811741189412537No Hit
GATCTTGGGTGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTC1910.12811741189412537No Hit
GATCTTGGGGTGCTGATCTTGGGGAGCATCTGGGACGTGAAGAGCCTGGAGT1890.1267758683140822No Hit
GATCTTGGGAAATTACACAACCTAACTCCCCCCAAAGAATCTGTATAAAGAA1890.1267758683140822No Hit
GATCTTGGGGCAAAGGGTTTTTGTGAATTACGGTGGTGGGTAAGACCCGAGT1890.1267758683140822No Hit
GATCTTGGGGCCTCTTGAGTTCTAGGTTCACCTGATCTGCAGAATGCTTTTC1870.12543432473403898No Hit
GATCTTGGGATTATCTCAAGACTTCCTCAAAAAAGAATTGGAGAACAATGAA1860.12476355294401739No Hit
GATCTTGGGACTTCCACGGAGTCTCAAACCTGCAGGAATTTCGTTTCATGAT1850.12409278115399577No Hit
GATCTTGGGGGGCTGTGCTGTGTGTGCGACCGATGCCCTGCGGCTCAAGTGG1850.12409278115399577No Hit
GATCTTGGGAGCCCATATCTGTGGAATGGAATATAAATATCACAAAGCCAAA1820.122080465783931No Hit
GATCTTGGGTATCGAGTGCAGCCCTGATCTTGGGAAGTCCTCTAATATGGTA1810.1214096939939094No Hit
GATCTTGGGTACGGGAACTTGCTTCCCTCTCCTGCCCCTCCTGCTGACTGGG1810.1214096939939094No Hit
GATCTTGGGATCAGCCAGTAAGATAATGTGCTTTTCTGGTTCGCTCAATAAA1810.1214096939939094No Hit
GATCTTGGAAGGAGGAAGAAGAATAGTCCTGAAGCAGGTGTGGAGAGGTCAG1810.1214096939939094No Hit
GATCTTGGGAGAGTGCAGTCCTGATCTTGGGTTCTGAAATTACTTTTGGGGT1800.12073892220388778No Hit
GATCTTGGGTGCTGATCTTGGGGTCACGAAATCCTTCGCTTAATTGCCGCAC1770.118726606833823No Hit
GATCTTGGGGAGGCAAGCTCTGGGGTGAAGACTGCACAGGCGCTCAGGTCAG1760.1180558350438014No Hit
GATCTTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA1760.1180558350438014No Hit
GATCTTGGGGAGAGACCCGGAGTCCGAATTCAGAGGCCCCAGTCTGTAGTCC1750.11738506325377979No Hit
GATCTTGGGGGCTCCTTCCCCAGGAGATTCATCTCCAAGAGAAGGAGCCGGA1750.11738506325377979No Hit
GATCTTGGGGAATTCTATGTTTTTAAAATTTTTTAATTTAAAAAAGTAAAAA1750.11738506325377979No Hit
GATCTTGGGGTTTAGTTTCAAGCGGACCGCGCCGGCTTCAGGCTATTGGAAG1740.1167142914637582No Hit
GATCTTGGGGAAATCATTTTCTTCAAGGTCTTTGCCCTTCTTGAACTTACGT1720.115372747883715No Hit
GATCTTGGGTAGTTGTGTTGTTTACAATACTCTGGAAGTAAACTGCTGCCCT1710.11470197609369341No Hit
GATCTTGGGCATATGGCACATCCCTGTAATCCCACCGCCTGGAAGGCAGCCT1710.11470197609369341No Hit
GATCTTGGGAGTGCAGTGCTGATCTTGGGGGGGGCTGGAGAGATGGCTCAGC1700.1140312043036718No Hit
GATCTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA1680.1126896607236286No Hit
GATCTTGGGGCACCTACCACATAAATTATAATGTTTAATGTTTTGAAAATGG1680.1126896607236286No Hit
GATCTTGGGGGCTGAGAAGAAGCCATTTGGTTTTGCAAAATGTGTACCATTT1680.1126896607236286No Hit
GATCTTGGGATCCGTGAGCGCCTGGGGCCTCTGGCGTCCCAGCCTCTGCGCG1680.1126896607236286No Hit
GATCTTGGGGCGGCTAGAATCTGCCAACGAGTCGCGTAGTAGCCCTATCGCC1660.11134811714358542No Hit
GATCTTAGGGATTGATTTGATAGACATGTTGAGTTACTACTTTGCTGTACAT1660.11134811714358542No Hit
GATCTTGGGTCATATCACAAGGTATGAATACACATAAAATTAATCACTGTTA1660.11134811714358542No Hit
GATCTTGGGATCTGATGTTACCATTGCGATCTAGCTTTCTTTTCATTAGCAT1650.11067734535356383No Hit
GATCTTGGGGGCCAACAGCTCCACGGGGTGGTCTGGCCCCACTGCGCGCTCC1640.11000657356354221No Hit
GATCTTGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAA1640.11000657356354221No Hit
GATCTTGGGGCCAAGCACAATTGTCCTCTGAGTGGAACCAAAGAAATGAAGA1610.10799425819347742No Hit
GATCTTGGGGTCTGTAGGCTGTGTGATTGCGGAAAATATAAATGCTGCTAAT1610.10799425819347742No Hit
GATCTTGGGGTGGGGTTCCAGAGTGGACCAGGACCAGAGAGTTGTCTTGGGT1600.10732348640345582No Hit
GATCTTGGGAGATCCTGGCCGCTGCAGCCCGCTCAGGCAGGGGACAGCGTCC1600.10732348640345582No Hit
GATCTTGGGGCTTTTAAAGGCGCTAGCTGGTGCGCTCTGTGACTTGATTGCT1600.10732348640345582No Hit
GATCTTGGGGAAGACATAGAAGACCTGGAACCCAGGGATGCCCAGTCCCACA1600.10732348640345582No Hit
GATCTTGGGTCATAAACTAAACAATTACCTACCACCTCCATCTCTTCCCCCT1590.10665271461343422No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAA1580.10598194282341261No Hit
GATCTTGGGATCCTTCTCCTGTCCTGCAACAACATCCATATCCAGCCAGGTG1580.10598194282341261No Hit
GATCTTGGGGTACCACTGTGAGGTGTAGGATAATGAAAGGCACAGTAGTCAA1570.10531117103339102No Hit
GATCTTGGGGCGGATGCCCCAGCTGCTCGCTACCAGACAATTGAAGAGAACA1570.10531117103339102No Hit
GATCTTGGGCAGCTCTCCCGGCAGACTAACCTCATAGTGGTCAAGTACTCCA1550.10396962745334783No Hit
GATCTTGGGATCGCCAGTGGGCAAACCTGATGGAGAAGATACAGGCCTCTGT1540.10329885566332621No Hit
GATCTTGGGAGCTCACGCGACTCTACGACTGCCAGGTGTGCTCCCCACCCCG1520.10195731208328303No Hit
GATCTTGGGATTGACCACAGTGGCGTCCGGCTCGGAGAGTATGAGGATGTGT1520.10195731208328303No Hit
GATCTTGGGGGCACAAGCGAAGCAGGACGTCAGACTAGAGAGTAGGGAGAGA1510.10128654029326142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAC351.0115764E-746.00000415
TCACGCG351.0115764E-746.00000413
GTCATAA351.0115764E-746.0000049
AGCAATG351.0115764E-746.00000415
GGCAGTT351.0115764E-746.00000425
GGTACTT351.0115764E-746.00000435
TCTGACC351.0115764E-746.00000413
TGCCCTG351.0115764E-746.00000434
TCGCAGT351.0115764E-746.00000412
ACTGCCA351.0115764E-746.00000428
TACTTGG351.0115764E-746.00000437
CATTGTT351.0115764E-746.00000425
ACACCAC351.0115764E-746.00000423
TTTCGCT351.0115764E-746.00000423
GGACTTC700.046.0000048
TAGGTCG351.0115764E-746.00000433
GACGTGA351.0115764E-746.00000434
TGGGTGT700.046.0000046
GCTATTT351.0115764E-746.00000427
CGGTTAC351.0115764E-746.00000430
Produced by FastQC (version 0.11.5)