FastQCFastQC Report
Fri 17 Jun 2016
SRR1527667_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527667_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150043
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGAAG17171.1443386229280939No Hit
GATCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC10130.6751397932592657No Hit
GATCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8990.5991615736822111No Hit
GATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8570.5711695980485594No Hit
GATCTTGGGGGCCCAGTTGATAAAGTGCTTGCTCTGGAAGCATGACCCATCC4060.2705890977919663No Hit
GATCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.24459654898928976No Hit
GATCTTGGGGGAGATGTGAGCATTTGCCAAGCTATGTATTCATCTGGATTGT3500.23326646361376407No Hit
GATCTTGGGAGAAATTCTGAGTATACTTGATTGTATCTTAAAAGTTAAAACT3470.23126703678278895No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGCAATATCAGAAGTTCCCTCTTG3460.2306005611724639No Hit
GATCTTGGGTGGCTTTGTGGCTAATGGGGAAAAATAACTATTTTAAAAGGTA3330.22193637823823836No Hit
GATCTTGGGATCTGCCCCCTCCCCCCCTCACAGCCAGCAGCATTTCCAAGTG3230.21527162213498796No Hit
GATCTTGGGAGCCCCTACACCAAGTATGCTAGCATGATCAACAAGGCCACAC3210.2139386709143379No Hit
GATCTTGGGATGGCTGTTAGCATAGAGTTTAAAAAAAGAGAGATAAGCTAAT3200.21327219530401284No Hit
GATCTTGGGACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG3200.21327219530401284No Hit
GATCTTGGGACACTAATTACTGTTTGTAGTGTCTTTGGATGCTCACGGGTTC3200.21327219530401284No Hit
GATCTTGGGATTGCAGGCTGATTCCTCCAGGAATATAATAGTGACGTTGAGT3170.21127276847303775No Hit
GATCTTGGGAGATGATGCATATATTGATATCTTTTATTGCAAAAATGTAAAT3120.20794039042141252No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAA3060.20394153675946228No Hit
GATCTTGGGAGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAA3050.20327506114913726No Hit
GATCTTGGGACCAGGAGACAGTAAAGGAGCTGCTGCAGGCAGGTTCTGCACA3040.20260858553881222No Hit
GATCTTGGGGATCTACAGTCACTTCAAACAAGCTACTGTGGGCGATGTAAAT2960.1972767806562119No Hit
GATCTTGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC2920.19461087821491171No Hit
GATCTTGGGAACGCAGAGTGCAGTGCTGATCTTGGGGAGAAGGAGAGAGAGA2920.19461087821491171No Hit
GATCTTGGGAGAGTGCAGTGCTGATCTTGGGAACCAGCTGTATCATTTGCCT2920.19461087821491171No Hit
GATCTTGGGTGCTGATCTTGGGAAAAGAAACTGTAAAAAGATCAGGGATAAC2920.19461087821491171No Hit
GATCTTGGGCAGGCAGTGGTGGCACACACCTTTGATCCCAGCACTTGGGAGG2910.19394440260458667No Hit
GATCTTGGGGTGTTATATGTGTTTACATTAAGATCTGAATTATTTAAAATTT2900.19327792699426163No Hit
GATCTTGGGGAATGCCCTTCAGCCTAGAGCGCGGAGGAGGACGGTAGCTCGG2870.1912785001632865No Hit
GATCTTGGGGCCTCTGCCTCTGCCTCTGCCTCTGCCTCTGCCTCTGCCTCTG2850.18994554894263643No Hit
GATCTTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA2840.18927907333231142No Hit
GATCTTGGGAATCAAGGTTAAAATATCACTAGCAATAAGACACATCAACACC2810.18727964650133627No Hit
GATCTTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTGAGAGCACTGACTGCT2720.18128136600841094No Hit
GATCTTGGGGCTAGGCACCCACACACCCATGTCCAACTCCTTTCTTTATCCA2720.18128136600841094No Hit
GATCTTGGGGAATTTGACTGATGCCAGGCGTGGTGGTGCACACCTTTAATCC2640.1759495611258106No Hit
GATCTTGGGTGCCGCAGTCAGTAACACAAATGGTGCGGCAACAAGCAGGCGA2640.1759495611258106No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGAAAAAAAAAAAAAAAAAAAAAAAA2620.1746166099051605No Hit
GATCTTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC2580.17195070746386035No Hit
GATCTTGGGGTTCGCAGTAAGCATTTGCACGGTTACTTAGTGAAACTTTTCT2580.17195070746386035No Hit
GATCTTGGGAGCTGGTCTTTGAATCTGCATGTACTTCACGTTTTCTATATTT2580.17195070746386035No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGGAGCCAGGCAGTGGTGGCGCATGC2570.17128423185353533No Hit
GATCTTGGGGCCCTGCGCCTAGTTACAGTTCCACTTCCTTTGATGCTCCGTA2560.17061775624321027No Hit
GATCTTGGGAGAAGGGGTCGGGGAGCATCAGGACTTGGGTTTTCTATGCTGT2560.17061775624321027No Hit
GATCTTGGGAGGCCTCTGTGGCTACCAACCCCATCATCACCCCAGTGGCCCA2530.16861832941223517No Hit
GATCTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG2490.165952426970935No Hit
GATCTTGGGGGAGCGCAGGGAACTGCACACAATACCTCCTAATAACCTACCT2470.16461947575028493No Hit
GATCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT2450.16328652452963485No Hit
GATCTTGGGATCTGCTCTCTGGTGATGTCTGCCTGTGAACGCACCTTATAAA2450.16328652452963485No Hit
GATCTTGGGAGCTGCTGACCTCTGCCAGGGCAGGTACCACTTCCTTGGGACC2440.16262004891930978No Hit
GATCTTGGGAGCATTCTTTTGGACACCACACCTAGGTCGGAGCACTACCGTC2440.16262004891930978No Hit
GATCTTGGGTACCCTAAAAAGGGTACCCAAATGAGGCGCTGCACTTAGTATA2420.16128709769865973No Hit
GATCTTGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.16062062208833466No Hit
GATCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGATCTTGGGGAG2390.15928767086768458No Hit
GATCTTGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAAT2350.15662176842638442No Hit
GATCTTGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG2340.1559552928160594No Hit
GATCTTGGGACGATACATACTATGTAGTAGCCCATTTCCACTATGTTCTATC2330.15528881720573434No Hit
GATCTTGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTG2320.15462234159540933No Hit
GATCTTGGGCAGAGTGCAGTGCTGATCTTGGGCACCCCAAAAGACGTAGAAA2290.15262291476443418No Hit
GATCTTGGGGTGCTTCTTTTGCAAGCCCTGCCTGAGACCCAAATCAACAGCT2290.15262291476443418No Hit
GATCTTGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT2260.1506234879334591No Hit
GATCTTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2260.1506234879334591Illumina Single End Adapter 2 (100% over 34bp)
GATCTTGGGGGGAGATGGAGGTGAGCCAGGCGGCGTCTGGTACCGACGGTGT2230.14862406110248394No Hit
GATCTTGGGAGGTGAGGGCTTTGGGAAGCCCTCCAGATAACTCTTGTTGCTC2200.14662463427150885No Hit
GATCTTGGGCGTAGTTCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCG2180.14529168305085877No Hit
GATCTTGGGGGTCTGTTCTGCACCAACTCAGAGTCTTGTTGGAGCTTTATCC2180.14529168305085877No Hit
GATCTTGGGGAAAGGAATCCCAAGAAAGCCAAAAGTGGTGCTACCTTTCCTC2170.14462520744053373No Hit
GATCTTGGGTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTGTCC2170.14462520744053373No Hit
GATCTTGGGGCTTCTTAAACCTGAAGTGGAGTACTACTCTGGTTGCGCTTGT2130.14195930499923357No Hit
GATCTTGGGTATGGTGCATTTTTTTCGCTATTTAAGTTGGATATTTCTCTAC2130.14195930499923357No Hit
GATCTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC2110.14062635377858348No Hit
GATCTTAGGGATTGATTTGATAGACATGTTGAGTTACTACTTTGCTGTACAT2090.1392934025579334No Hit
GATCTTGGGATGGCCGTACACCCACCCTCTAAGGCCACCAGTTGTCTCTAGA2080.13862692694760834No Hit
GATCTTGGGGCCTTTTCCGGTCGCGGCGCTGCGCGGTGTGGAGCTCGTGCTT2070.13796045133728332No Hit
GATCTTGGGGCAGCGGGCAACCTGCCAAGCCTTTAGCTTTCTCTGGTCTATA2060.13729397572695826No Hit
GATCTTGGGTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGTGCCATGA2030.13529454889598316No Hit
GATCTTGGGGTGCTGATCTTGGGGGGCAGTTGCTGGTACTTGGCCCTCAGCC2000.13329512206500801No Hit
GATCTTGGGGTTTAGTTTCAAGCGGACCGCGCCGGCTTCAGGCTATTGGAAG1990.132628646454683No Hit
GATCTTGGGATTATCTCAAGACTTCCTCAAAAAAGAATTGGAGAACAATGAA1980.13196217084435796No Hit
GATCTTGGGCTTCTTAATGAAGAAAGCAGAATCAGACACCTAAGACCAATCA1960.13062921962370788No Hit
GATCTTGGGGTGCTGATCTTGGGGAGCATCTGGGACGTGAAGAGCCTGGAGT1960.13062921962370788No Hit
GATCTTGGGATGAGGGCCAAGCCCAGGTTGCTTCTATTTGCTTCACCTTTAA1960.13062921962370788No Hit
GATCTTGGGAGCCCATATCTGTGGAATGGAATATAAATATCACAAAGCCAAA1940.1292962684030578No Hit
GATCTTGGGAGAGTGCAGTCCTGATCTTGGGTTCTGAAATTACTTTTGGGGT1940.1292962684030578No Hit
GATCTTGGGGAGAAGCGGCTCATTCCTGATGGCTGTGGTGTCAAATATATCC1910.12729684157208265No Hit
GATCTTGGCGCAGAGTGCAGTGCTGATCTTGGGGGGTGGCTGGAGAGATGGC1900.12663036596175764No Hit
GATCTTGGGGGCCAACAGCTCCACGGGGTGGTCTGGCCCCACTGCGCGCTCC1890.12596389035143257No Hit
GATCTTGGGGAGAGACCCGGAGTCCGAATTCAGAGGCCCCAGTCTGTAGTCC1880.12529741474110756No Hit
GATCTTGGGGAGGCAAGCTCTGGGGTGAAGACTGCACAGGCGCTCAGGTCAG1860.12396446352045748No Hit
GATCTTGGGGAATTCTATGTTTTTAAAATTTTTTAATTTAAAAAAGTAAAAA1850.12329798791013243No Hit
GATCTTGGAAGGAGGAAGAAGAATAGTCCTGAAGCAGGTGTGGAGAGGTCAG1850.12329798791013243No Hit
GATCTTGGGGCTTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACAC1840.1226315122998074No Hit
GATCTTGGGACTTCCACGGAGTCTCAAACCTGCAGGAATTTCGTTTCATGAT1820.1212985610791573No Hit
GATCTTGGGGCCTCTTGAGTTCTAGGTTCACCTGATCTGCAGAATGCTTTTC1820.1212985610791573No Hit
GATCTTGGGTGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTC1820.1212985610791573No Hit
GATCTTGGGATCTGATGTTACCATTGCGATCTAGCTTTCTTTTCATTAGCAT1810.12063208546883226No Hit
GATCTTGGGTGCTGATCTTGGGGTCACGAAATCCTTCGCTTAATTGCCGCAC1790.11929913424818218No Hit
GATCTTGGGCAAATTGTGTGACACATATTCATGCATAAACTGTTAAAACGAG1780.11863265863785714No Hit
GATCTTGGGCTGCTTTGCTTTCCCTTTCCACCTCAAGACTTCAAGAAGACAG1750.11663323180688204No Hit
GATCTTGGCGCAGGGCCTTTGTTCTACCTCTGAGGACAGAGAGCAGTTTCCC1730.11530028058623196No Hit
GATCTTGGGGCAAAGGGTTTTTGTGAATTACGGTGGTGGGTAAGACCCGAGT1730.11530028058623196No Hit
GATCTTGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAAA1730.11530028058623196No Hit
GATCTTGGGTAGTTGTGTTGTTTACAATACTCTGGAAGTAAACTGCTGCCCT1720.1146338049759069No Hit
GATCTTGGGGGGCTGTGCTGTGTGTGCGACCGATGCCCTGCGGCTCAAGTGG1720.1146338049759069No Hit
GATCTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA1710.11396732936558188No Hit
GATCTTGGGTATCGAGTGCAGCCCTGATCTTGGGAAGTCCTCTAATATGGTA1710.11396732936558188No Hit
GATCTTGGGAAATTACACAACCTAACTCCCCCCAAAGAATCTGTATAAAGAA1710.11396732936558188No Hit
GATCTTGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA1710.11396732936558188No Hit
GATCTTGGGATCCTTCTCCTGTCCTGCAACAACATCCATATCCAGCCAGGTG1680.11196790253460674No Hit
GATCTTGGGCATATGGCACATCCCTGTAATCCCACCGCCTGGAAGGCAGCCT1670.11130142692428172No Hit
GATCTTGGGTGGGGCAGTCCTTGCTTCCTGCTTGCTATCCCACAGGAATCTG1640.10930200009330658No Hit
GATCTTGGGGCGGCTAGAATCTGCCAACGAGTCGCGTAGTAGCCCTATCGCC1630.10863552448298155No Hit
GATCTTGGGGAGTGCAGTGCTGATCTTGGGGAAAAAAAAAAAAAAAAAAAAA1630.10863552448298155No Hit
GATCTTGGGGTGCTGATCTTGGGCAAAGGCAGAGCAGCTGGTTGTGACATTT1630.10863552448298155No Hit
GATCTTGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTT1620.1079690488726565No Hit
GATCTTGGGATCCGTGAGCGCCTGGGGCCTCTGGCGTCCCAGCCTCTGCGCG1620.1079690488726565No Hit
GATCTTGGGGGCTCCTTCCCCAGGAGATTCATCTCCAAGAGAAGGAGCCGGA1610.10730257326233147No Hit
GATCTTGGGTCATAAACTAAACAATTACCTACCACCTCCATCTCTTCCCCCT1610.10730257326233147No Hit
GATCTTGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTG1600.10663609765200642No Hit
GATCTTGGGATCAGCCAGTAAGATAATGTGCTTTTCTGGTTCGCTCAATAAA1600.10663609765200642No Hit
GATCTTGGGGTCCCTAGGTAGGCTTGGTCATTTGTGGGTTTTTCCCAGGCCT1580.10530314643135634No Hit
GATCTTGGGGTGCAGTGCTGATCTTGGGATTGACAGTATTGTAAACAGTCTG1570.1046366708210313No Hit
GATCTTGGGGACTGCTTCGACCTCTGCCGCCGCCACTGCCGCCGCCTCGGGA1550.10330371960038122No Hit
GATCTTGGGGCTTTTAAAGGCGCTAGCTGGTGCGCTCTGTGACTTGATTGCT1530.10197076837973114No Hit
GATCTTGGGGAAGACATAGAAGACCTGGAACCCAGGGATGCCCAGTCCCACA1530.10197076837973114No Hit
GATCTTGGGGTCTGTTCTGCACCAACTCAGAGTCTTGTTGGAGCTTTATCCC1520.10130429276940611No Hit
GATCTTGGGGCGGATGCCCCAGCTGCTCGCTACCAGACAATTGAAGAGAACA1520.10130429276940611No Hit
GATCTTGGGAAGTCTTTCAGATCTCTTTGCTTTGAAACTTCGTCGATTCCAT1520.10130429276940611No Hit
GATCTTGGGAGACCCAGCAAATACGCCTGCAGGCGGAGATCTTCCAGGCCCG1510.10063781715908106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATTC351.01163096E-746.00000411
TGCCCTG351.01163096E-746.00000434
GATGTAA351.01163096E-746.00000444
CTACTGT351.01163096E-746.00000433
GATAACT351.01163096E-746.00000436
TAAAGTG351.01163096E-746.00000421
TAAAGCC351.01163096E-746.00000417
AGATAAC351.01163096E-746.00000435
CCGATGC351.01163096E-746.00000430
TTTCGCT351.01163096E-746.00000423
CAGTTGA351.01163096E-746.00000414
GCTATTT351.01163096E-746.00000427
CGTGAAG351.01163096E-746.00000436
ATGTGAG351.01163096E-746.00000414
GCTATGT351.01163096E-746.00000431
GACCGAT351.01163096E-746.00000428
ACCGATG351.01163096E-746.00000429
CCCAGTT351.01163096E-746.00000412
ACTTCAT351.01163096E-746.00000410
GCGCCAC351.01163096E-746.00000414
Produced by FastQC (version 0.11.5)