FastQCFastQC Report
Fri 17 Jun 2016
SRR1527666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2005331
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAA594222.9632015861720586No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAA348061.7356735621201684No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA329231.6417738517980323No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAA298221.487136038888343No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA206231.0284087764064884No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA194790.971360837687145No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAA177720.8862377333218306No Hit
TAGTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA137570.6860214099318267Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGA119690.5968590721432023No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAA115150.5742194181409453No Hit
TAGTCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA78840.39315205320218954Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA66950.33386009591434035Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA64320.3207450540584073Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAA60970.3040395824928653No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAA49090.24479749228431616No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAGTGCAGTGCTTAGTCGGG46200.23038590636657988No Hit
TAGTCGGGGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAA42820.21353083356313746No Hit
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAA39860.1987701780903003No Hit
TAGTCGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA37140.18520633252066618Illumina Single End Adapter 2 (100% over 34bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGCAAAAAAAAAAAAAAAAAAAA36760.1833113835072614No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGATCGGAAGAGCTCGTATGCCG34370.17139315155453141Illumina Single End Adapter 2 (95% over 23bp)
TAGTCGGGGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA32710.16311521639071055No Hit
TAGTCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA32670.16291574807351006Illumina Single End Adapter 2 (96% over 32bp)
TAGTCGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA31530.15723090103329573No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGTAAAAAAAAAAAAAAAAAAAA30160.15039911116917853No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAA29150.14536253615986588No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGAAGAGCTCGTATGCCGTCTTCT28670.14296891635345985Illumina Single End Adapter 2 (96% over 27bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGCAAAAAAAAAAAAAAAAAAA28140.1403259611505532No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAA27970.13947822080245106No Hit
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAA27380.13653606312374367No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGAAAAAAAAAAAAAAAAAAAAAAA27010.13469098118963901No Hit
TAGTCGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA26310.13120028563863023Illumina Single End Adapter 2 (100% over 34bp)
TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24990.12461783117101366No Hit
TAGTCGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA24830.12381995790221166Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAA24760.12347088834711079No Hit
TAGTCGGGAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAA22930.11434521283518781No Hit
TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21180.10561847395766583No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGAAAAAAAAAAAAAAAAAAAAAA21080.1051198031646646No Hit
TAGTCGGGGGAGTGCAGTGCTTGATCGGAAGAGCTCGTATGCCGTCTTCT20750.10347418954776044Illumina Single End Adapter 2 (96% over 29bp)
TAGTCGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG20600.10272618335825856Illumina Single End Adapter 2 (100% over 23bp)
TAGTCGGGGAAAAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCTTCT20590.10267631627895843Illumina Single End Adapter 2 (100% over 29bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGATCGGAAGAGCTCGTATGCCGT20490.10217764548595717Illumina Single End Adapter 2 (95% over 24bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGCAAAAAAAAAAAAAAAAAAAAA20170.10058189894835316No Hit
TAGTCGGGGAAAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCTTCTG20090.10018296231395216Illumina Single End Adapter 2 (100% over 30bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAC207.857886E-444.021
ACCGGTG207.857886E-444.042
TAGCGTC207.857886E-444.024
CGGCATC207.857886E-444.05
TAGCGAG254.4440312E-544.027
ACCGACG207.857886E-444.031
CCTAACT207.857886E-444.027
CATTAGC351.446806E-744.010
ACTAGGT351.446806E-744.029
TATCTAT207.857886E-444.018
TACGAAC302.5286645E-644.023
ACGTGAC302.5286645E-644.032
CTATTGA302.5286645E-644.040
TAAGGGC207.857886E-444.014
CACACGT254.4440312E-544.039
CCGTTCA207.857886E-444.013
TACCGTA207.857886E-444.037
TGCCGAA207.857886E-444.025
CTAGCGT207.857886E-444.023
GTAACTA501.3496901E-939.614
Produced by FastQC (version 0.11.5)