##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527666_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2005331
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82813161517974	31.0	31.0	34.0	30.0	34.0
2	31.99881864889138	33.0	31.0	34.0	30.0	34.0
3	32.509143876995864	34.0	31.0	34.0	30.0	34.0
4	35.435264801671146	35.0	35.0	37.0	33.0	37.0
5	35.98304070500082	37.0	35.0	37.0	35.0	37.0
6	36.18298924217498	37.0	35.0	37.0	35.0	37.0
7	36.51323995888958	37.0	37.0	37.0	35.0	37.0
8	36.527118964400394	37.0	37.0	37.0	35.0	37.0
9	37.99468317200502	39.0	39.0	39.0	35.0	39.0
10	37.414628308244374	39.0	37.0	39.0	35.0	39.0
11	36.92896634022014	39.0	37.0	39.0	32.0	39.0
12	37.2005943158511	39.0	37.0	39.0	34.0	39.0
13	36.52583139641286	39.0	35.0	39.0	32.0	39.0
14	37.87377395552156	40.0	38.0	40.0	33.0	40.0
15	38.09226456879188	40.0	38.0	40.0	34.0	40.0
16	37.89334478946368	40.0	38.0	40.0	33.0	40.0
17	38.06541164525956	40.0	38.0	40.0	34.0	40.0
18	37.80775941727326	40.0	38.0	40.0	33.0	40.0
19	37.91583933026518	40.0	38.0	40.0	34.0	40.0
20	38.044859427196805	40.0	38.0	40.0	34.0	40.0
21	38.08372533013253	40.0	38.0	40.0	34.0	40.0
22	37.764929081533175	39.0	38.0	40.0	34.0	40.0
23	36.80889987737685	38.0	37.0	40.0	33.0	40.0
24	36.23416383629436	37.0	35.0	40.0	32.0	40.0
25	35.76510012561517	37.0	35.0	39.0	31.0	40.0
26	35.81266783388877	37.0	35.0	40.0	31.0	40.0
27	35.67140038228103	37.0	35.0	40.0	30.0	40.0
28	35.30935092510912	37.0	35.0	40.0	29.0	40.0
29	34.86368335202518	37.0	35.0	40.0	26.0	40.0
30	34.53129184159623	36.0	34.0	40.0	24.0	40.0
31	34.112932977149406	36.0	33.0	40.0	23.0	40.0
32	33.923959685458414	36.0	33.0	40.0	21.0	40.0
33	33.62834614335489	36.0	33.0	40.0	20.0	40.0
34	32.776979959916844	35.0	33.0	39.0	15.0	40.0
35	32.116675501450885	35.0	32.0	39.0	12.0	40.0
36	31.18496098649051	35.0	30.0	39.0	10.0	40.0
37	30.165751190202513	35.0	26.0	37.0	9.0	40.0
38	29.43026562697131	35.0	23.0	37.0	8.0	40.0
39	28.791089850004813	35.0	21.0	36.0	8.0	40.0
40	28.24406394754781	35.0	18.0	35.0	8.0	40.0
41	27.887240061615763	35.0	17.0	35.0	8.0	39.0
42	27.598925065238607	35.0	17.0	35.0	8.0	39.0
43	27.34228214693734	34.0	15.0	35.0	8.0	39.0
44	26.89757950183785	34.0	14.0	35.0	8.0	38.0
45	26.752487245247792	34.0	13.0	35.0	8.0	38.0
46	26.53091085711037	33.0	12.0	35.0	8.0	38.0
47	26.339775827531714	33.0	11.0	35.0	8.0	37.0
48	25.98442950315933	33.0	10.0	35.0	8.0	37.0
49	25.610692199941056	33.0	10.0	35.0	8.0	36.0
50	24.750326504701718	32.0	10.0	35.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	21.0
12	41.0
13	51.0
14	65.0
15	148.0
16	241.0
17	567.0
18	1334.0
19	2998.0
20	5352.0
21	9076.0
22	15210.0
23	25043.0
24	37414.0
25	51116.0
26	69435.0
27	94830.0
28	117541.0
29	110610.0
30	81278.0
31	63580.0
32	69228.0
33	86695.0
34	124821.0
35	175951.0
36	347951.0
37	353364.0
38	137229.0
39	24135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	98.49815317271812	0.9501174619052914	0.36123712245010925	0.19049224292647945
8	94.85905319371216	3.4699009789406334	0.8346253062462008	0.8364205211010053
9	80.79673629939397	13.825348533483997	3.3480258371311273	2.029889329990909
10	35.566996171704325	47.73785474816876	9.126323783953872	7.568825296173051
11	18.646298291902934	62.23675792175955	12.526261250636427	6.590682535701088
12	40.73641708027254	39.050710331611086	13.356597988062818	6.856274600053558
13	18.134412722887145	40.40749382520891	23.98591554212247	17.47217790978148
14	27.935737292247513	49.778266031891995	8.94685216555272	13.339144510307774
15	21.055177424574794	47.03358198721308	9.214039976442793	22.69720061176933
16	21.232205556090243	54.47594437028101	17.88622426921042	6.405625804418323
17	35.49079927453373	39.840305665249275	16.514530518901868	8.154364541315125
18	22.09400842055501	41.43141456447838	24.33029759176914	12.144279423197467
19	27.157461785610455	38.08942264394257	19.980940802291492	14.772174768155482
20	12.870344097807296	40.70106132104874	24.320024973433313	22.10856960771065
21	12.516088366459202	42.50595038923749	33.9285634142194	11.049397830083912
22	18.726584289576135	44.156650448230245	24.876591445502015	12.240173816691609
23	20.030957482829518	54.57468118729526	17.545831585907763	7.848529743967454
24	27.50189370233642	41.5965743311204	18.742541754952175	12.158990211591005
25	20.189085991290217	37.81425610036448	23.553518097511084	18.44313981083422
26	29.759625717649605	37.22263307154779	11.112828754953672	21.904912455848937
27	37.83355466005362	38.094259750634684	11.4023071502909	12.66987843902079
28	36.07863240532361	43.630851964089715	9.292081955547488	10.998433675039184
29	35.518824573100396	44.98484290124672	10.56364261062139	8.932689915031483
30	25.64334765682074	54.32295217098822	10.893812542667519	9.139887629523505
31	20.177516828892585	57.42787599653124	11.245026382178304	11.149580792397863
32	16.592422896768664	59.21062408151073	14.996626492085346	9.200326529635257
33	19.15354622254381	58.516374603494384	12.80451955313113	9.525559620830675
34	17.10784902841476	58.257714063164634	12.043448188852613	12.590988719567992
35	17.380572085107147	60.77096499281166	12.055665623281143	9.79279729880005
36	20.431589597926727	56.75152879998364	11.675877947331388	11.141003654758242
37	19.705973727030603	54.8677500123421	14.34660911340821	11.079667147219087
38	22.509899861918058	51.90798925464175	13.948270883958807	11.633839999481383
39	21.249359831369485	49.949908518843024	14.647906006539568	14.152825643247924
40	22.445870532096695	48.43449784599151	16.409859519450904	12.709772102460889
41	21.437159252013757	49.0271680834735	16.814481000892123	12.721191663620617
42	21.26691304328313	51.36319141328788	14.761054409471551	12.608841133957435
43	23.59610458323339	49.40900030967456	14.633743756018333	12.361151351073712
44	21.699460089132415	49.799010736880845	15.813050314387	12.688478859599735
45	21.10858506650523	49.196317216459526	14.58487401830421	15.110223698731032
46	22.400491489933582	48.8694385116472	14.845628975964567	13.884441022454647
47	22.498879237392728	48.21548163370536	14.783743930553111	14.501895198348802
48	22.313273968237663	47.7567543712235	15.187617405804826	14.742354254734005
49	21.66909103783864	47.56361917309412	15.54606197181413	15.22122781725311
50	19.78855361035161	47.808167329981934	17.068852972402063	15.334426087264397
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	249.0
6	498.0
7	1350.0
8	2202.0
9	13462.5
10	24723.0
11	33587.5
12	42452.0
13	27513.0
14	12574.0
15	11768.5
16	10963.0
17	12802.0
18	14641.0
19	17673.0
20	20705.0
21	24837.0
22	28969.0
23	34756.5
24	40544.0
25	48405.5
26	56267.0
27	65100.5
28	73934.0
29	81790.5
30	89647.0
31	94953.5
32	100260.0
33	118076.0
34	135892.0
35	165129.0
36	194366.0
37	182199.0
38	170032.0
39	131780.5
40	93529.0
41	84850.0
42	76171.0
43	77131.5
44	78092.0
45	73992.5
46	69893.0
47	66127.0
48	62361.0
49	56306.5
50	50252.0
51	52469.5
52	54687.0
53	66550.0
54	78413.0
55	97738.0
56	117063.0
57	111087.5
58	105112.0
59	97607.5
60	90103.0
61	73146.5
62	56190.0
63	40033.0
64	23876.0
65	17917.0
66	11958.0
67	9684.5
68	7411.0
69	5704.5
70	3998.0
71	3802.0
72	3606.0
73	2855.0
74	2104.0
75	1525.0
76	946.0
77	756.5
78	567.0
79	364.5
80	162.0
81	140.5
82	119.0
83	73.0
84	27.0
85	19.0
86	11.0
87	9.0
88	7.0
89	5.0
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2005331.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.13289456516397
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.27574798446105	26.266349995436585
2	10.016166973620868	6.637292085681154
3	3.601658257049639	3.580000898717337
4	1.7580438776870797	2.329963297613522
5	1.022873476291768	1.6945379521738948
6	0.6966205683969174	1.3848633506771797
7	0.5250099401924043	1.217656929584058
8	0.3805571610316843	1.0087168233984738
9	0.29525476696332964	0.8804380557292255
>10	1.992473631437634	13.024557956925955
>50	0.250983595206775	5.75558838868964
>100	0.15218457255783613	9.673940265250602
>500	0.017283240786063702	4.05305115548941
>1k	0.013000667847924023	8.344382941308172
>5k	6.11796134019954E-4	1.373736989997461
>10k+	0.0015294903350498853	12.77492291332743
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAA	59422	2.9632015861720586	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAA	34806	1.7356735621201684	No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32923	1.6417738517980323	No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAA	29822	1.487136038888343	No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20623	1.0284087764064884	No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA	19479	0.971360837687145	No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAA	17772	0.8862377333218306	No Hit
TAGTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	13757	0.6860214099318267	Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGA	11969	0.5968590721432023	No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAA	11515	0.5742194181409453	No Hit
TAGTCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	7884	0.39315205320218954	Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	6695	0.33386009591434035	Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	6432	0.3207450540584073	Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAA	6097	0.3040395824928653	No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAA	4909	0.24479749228431616	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAGTGCAGTGCTTAGTCGGG	4620	0.23038590636657988	No Hit
TAGTCGGGGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	4282	0.21353083356313746	No Hit
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAA	3986	0.1987701780903003	No Hit
TAGTCGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	3714	0.18520633252066618	Illumina Single End Adapter 2 (100% over 34bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGCAAAAAAAAAAAAAAAAAAAA	3676	0.1833113835072614	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGATCGGAAGAGCTCGTATGCCG	3437	0.17139315155453141	Illumina Single End Adapter 2 (95% over 23bp)
TAGTCGGGGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	3271	0.16311521639071055	No Hit
TAGTCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA	3267	0.16291574807351006	Illumina Single End Adapter 2 (96% over 32bp)
TAGTCGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA	3153	0.15723090103329573	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGTAAAAAAAAAAAAAAAAAAAA	3016	0.15039911116917853	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAA	2915	0.14536253615986588	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGAAGAGCTCGTATGCCGTCTTCT	2867	0.14296891635345985	Illumina Single End Adapter 2 (96% over 27bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGCAAAAAAAAAAAAAAAAAAA	2814	0.1403259611505532	No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAA	2797	0.13947822080245106	No Hit
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAA	2738	0.13653606312374367	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGAAAAAAAAAAAAAAAAAAAAAAA	2701	0.13469098118963901	No Hit
TAGTCGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	2631	0.13120028563863023	Illumina Single End Adapter 2 (100% over 34bp)
TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2499	0.12461783117101366	No Hit
TAGTCGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	2483	0.12381995790221166	Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAA	2476	0.12347088834711079	No Hit
TAGTCGGGAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAA	2293	0.11434521283518781	No Hit
TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2118	0.10561847395766583	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGAAAAAAAAAAAAAAAAAAAAAA	2108	0.1051198031646646	No Hit
TAGTCGGGGGAGTGCAGTGCTTGATCGGAAGAGCTCGTATGCCGTCTTCT	2075	0.10347418954776044	Illumina Single End Adapter 2 (96% over 29bp)
TAGTCGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG	2060	0.10272618335825856	Illumina Single End Adapter 2 (100% over 23bp)
TAGTCGGGGAAAAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCTTCT	2059	0.10267631627895843	Illumina Single End Adapter 2 (100% over 29bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGATCGGAAGAGCTCGTATGCCGT	2049	0.10217764548595717	Illumina Single End Adapter 2 (95% over 24bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGCAAAAAAAAAAAAAAAAAAAAA	2017	0.10058189894835316	No Hit
TAGTCGGGGAAAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCTTCTG	2009	0.10018296231395216	Illumina Single End Adapter 2 (100% over 30bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.09250343210173283	0.0	0.0	0.0	0.0
8	0.14232064432255823	0.0	0.0	0.0	0.0
9	0.24609403634611943	0.0	0.0	0.0	0.0
10	0.5339268180664439	0.0	0.0	0.0	0.0
11	0.9114704754476942	0.0	0.0	0.0	0.0
12	1.0979234849508634	0.0	0.0	0.0	0.0
13	1.2324149978233019	0.0	0.0	0.0	0.0
14	1.499403340396174	0.0	0.0	0.0	0.0
15	1.7510326225446073	0.0	0.0	0.0	0.0
16	2.040112081247435	0.0	0.0	0.0	0.0
17	2.2242712051027986	0.0	0.0	0.0	0.0
18	2.4248864651272033	0.0	0.0	0.0	0.0
19	2.6395642415142437	0.0	0.0	0.0	0.0
20	2.8710472236254265	0.0	0.0	0.0	0.0
21	3.1208812909190553	0.0	0.0	0.0	0.0
22	3.403228693916366	0.0	0.0	0.0	0.0
23	3.715446477414452	0.0	0.0	0.0	0.0
24	3.999240025711466	0.0	0.0	0.0	0.0
25	4.250520238304799	0.0	0.0	0.0	0.0
26	4.527132927182595	0.0	0.0	0.0	0.0
27	4.756272156566672	0.0	0.0	0.0	0.0
28	5.075720666563275	0.0	0.0	0.0	0.0
29	5.26810785850316	0.0	0.0	0.0	0.0
30	5.498294296552539	0.0	0.0	0.0	0.0
31	5.687789197893016	0.0	0.0	0.0	9.973415860025103E-5
32	5.875788086854489	0.0	0.0	0.0	9.973415860025103E-5
33	6.042393998796209	0.0	0.0	0.0	9.973415860025103E-5
34	6.2119420684166355	0.0	0.0	0.0	9.973415860025103E-5
35	6.467261514433278	0.0	0.0	0.0	9.973415860025103E-5
36	6.645935259565628	0.0	0.0	0.0	9.973415860025103E-5
37	6.8475977282553355	0.0	0.0	0.0	9.973415860025103E-5
38	7.051155145958448	0.0	0.0	0.0	1.4960123790037656E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATAC	20	7.857886E-4	44.0	21
ACCGGTG	20	7.857886E-4	44.0	42
TAGCGTC	20	7.857886E-4	44.0	24
CGGCATC	20	7.857886E-4	44.0	5
TAGCGAG	25	4.4440312E-5	44.0	27
ACCGACG	20	7.857886E-4	44.0	31
CCTAACT	20	7.857886E-4	44.0	27
CATTAGC	35	1.446806E-7	44.0	10
ACTAGGT	35	1.446806E-7	44.0	29
TATCTAT	20	7.857886E-4	44.0	18
TACGAAC	30	2.5286645E-6	44.0	23
ACGTGAC	30	2.5286645E-6	44.0	32
CTATTGA	30	2.5286645E-6	44.0	40
TAAGGGC	20	7.857886E-4	44.0	14
CACACGT	25	4.4440312E-5	44.0	39
CCGTTCA	20	7.857886E-4	44.0	13
TACCGTA	20	7.857886E-4	44.0	37
TGCCGAA	20	7.857886E-4	44.0	25
CTAGCGT	20	7.857886E-4	44.0	23
GTAACTA	50	1.3496901E-9	39.6	14
>>END_MODULE