FastQCFastQC Report
Fri 17 Jun 2016
SRR1527665_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527665_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2839316
Sequences flagged as poor quality0
Sequence length52
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA782222.754959293012824No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAA455351.6037313212055297No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA438331.543787306520303No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAA392451.382199093020995No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA274670.9673808762392069No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA253940.8943703342636043No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAA230980.8135057880137329No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGAAG164140.5780969782863197No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAA147570.5197378523559899No Hit
TAGTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA115340.406224597755234Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAA80890.284892558630318No Hit
TAGTCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA71570.25206775152888933Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA70330.24770050251539452Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAA60960.21469959666342175No Hit
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAA54760.19286335159594775No Hit
TAGTCGGGGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA50440.17764841954893362No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGCAAAAAAAAAAAAAAAAAAAAAA49530.17444342228903018No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGATCGGAAGAGCTCGTATGCCGTC47040.16567370451193175Illumina Single End Adapter 2 (96% over 25bp)
TAGTCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA46950.16535672676095228Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA40840.14383745944445775No Hit
TAGTCGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA40130.1413368571867309Illumina Single End Adapter 2 (100% over 34bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGTAAAAAAAAAAAAAAAAAAAAAA39220.13813185992682744No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAAAA38870.13689916867301843No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGAAGAGCTCGTATGCCGTCTTCTGC37880.13341241341224436Illumina Single End Adapter 2 (96% over 29bp)
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAA37330.13147532715625876No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGCAAAAAAAAAAAAAAAAAAAAA36120.12721373739309044No Hit
TAGTCGGGGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA35960.12665022139134918No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA34350.12097984162382772No Hit
TAGTCGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA32380.11404155085238839Illumina Single End Adapter 2 (100% over 34bp)
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAA32190.11337237560032064No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGAAAAAAAAAAAAAAAAAAAAAAAAA32060.11291451884890585No Hit
TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31170.10977996108922007No Hit
TAGTCGGGAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA30560.10763155633258151No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAGTGCAGTGCTTAGTCGGGGA29820.10502529482452816No Hit
TAGTCGGGGGAGTGCAGTGCTTGATCGGAAGAGCTCGTATGCCGTCTTCTGC28810.10146810006353643Illumina Single End Adapter 2 (96% over 31bp)
TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28640.10086936431168633No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGATCGGAAGAGCTCGTATGCCGTCT28460.10023540880972741Illumina Single End Adapter 2 (96% over 26bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCT351.02028935E-746.00000439
TCTAAGT351.02028935E-746.00000426
TAGACCG453.110472E-1046.041
GTAATAG206.3130306E-446.018
GTAGCGA253.4184333E-546.029
CATAAGT253.4184333E-546.041
CGATGTA253.4184333E-546.035
ACGAACT508.731149E-1041.424
TCACGCC451.5897967E-840.8888933
CACGGTT451.5897967E-840.8888919
AATAGCG451.5897967E-840.8888916
CGGCGTA402.9126022E-740.2513
TTAGCGA402.9126022E-740.2526
TCCCGAC402.9126022E-740.2544
ATACACG402.9126022E-740.2528
GAAGCAG131350.040.0464449
AAGCAGT132050.039.83415210
CGGGTAT2950.039.762715
GGAAGCA137650.039.6505628
TAATAGT355.375905E-639.42857437
Produced by FastQC (version 0.11.5)