##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527665_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2839316 Sequences flagged as poor quality 0 Sequence length 52 %GC 41 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.61697958240647 31.0 31.0 33.0 30.0 34.0 2 31.689613273055905 31.0 31.0 34.0 30.0 34.0 3 32.20033064301402 34.0 31.0 34.0 30.0 34.0 4 35.20332784374828 35.0 35.0 37.0 33.0 37.0 5 35.684797676623525 37.0 35.0 37.0 35.0 37.0 6 35.91768686542815 37.0 35.0 37.0 35.0 37.0 7 36.45857417772449 37.0 37.0 37.0 35.0 37.0 8 36.467670382585105 37.0 37.0 37.0 35.0 37.0 9 37.966219681078115 39.0 39.0 39.0 35.0 39.0 10 37.20548294025744 39.0 37.0 39.0 34.0 39.0 11 36.66869555907127 39.0 37.0 39.0 32.0 39.0 12 36.90790352324292 39.0 37.0 39.0 33.0 39.0 13 36.24616879558316 38.0 35.0 39.0 30.0 39.0 14 37.57862492234045 39.0 37.0 40.0 33.0 40.0 15 37.82622927493805 40.0 38.0 40.0 33.0 40.0 16 37.641809506233194 40.0 37.0 40.0 33.0 40.0 17 37.79811687040118 40.0 38.0 40.0 33.0 40.0 18 37.62328250888594 40.0 37.0 40.0 33.0 40.0 19 37.67120038769901 40.0 37.0 40.0 33.0 40.0 20 37.86824502802788 40.0 38.0 40.0 33.0 40.0 21 37.8992539048137 40.0 38.0 40.0 33.0 40.0 22 37.60636399752617 39.0 37.0 40.0 33.0 40.0 23 36.755669675372516 38.0 36.0 40.0 32.0 40.0 24 36.1567296489718 37.0 35.0 40.0 31.0 40.0 25 35.76525120838963 37.0 35.0 40.0 30.0 40.0 26 35.70469648323751 37.0 35.0 40.0 30.0 40.0 27 35.527348840354506 37.0 35.0 40.0 30.0 40.0 28 35.21107794975973 37.0 35.0 40.0 28.0 40.0 29 34.8018441765552 36.0 35.0 40.0 26.0 40.0 30 34.16733361133456 36.0 33.0 40.0 23.0 40.0 31 33.611930126833364 35.0 33.0 39.0 21.0 40.0 32 33.24567290150163 35.0 33.0 39.0 18.0 40.0 33 33.00851507898381 35.0 33.0 39.0 18.0 40.0 34 32.29208443160254 35.0 31.0 39.0 15.0 40.0 35 31.58081242101971 35.0 31.0 38.0 11.0 40.0 36 30.70006226851819 35.0 29.0 38.0 10.0 40.0 37 29.642809747136283 35.0 24.0 37.0 9.0 40.0 38 28.950351422666586 35.0 22.0 36.0 8.0 40.0 39 28.310800559007873 35.0 20.0 35.0 8.0 39.0 40 27.77093285847718 34.0 18.0 35.0 8.0 39.0 41 27.429997576881192 34.0 17.0 35.0 8.0 38.0 42 27.077626442424865 34.0 15.0 35.0 8.0 38.0 43 26.78646371168267 33.0 15.0 35.0 8.0 38.0 44 26.45190390925138 33.0 13.0 35.0 8.0 38.0 45 26.285661757972694 33.0 12.0 35.0 8.0 37.0 46 26.062415032352863 33.0 11.0 35.0 8.0 37.0 47 25.869797866810174 33.0 10.0 35.0 8.0 36.0 48 25.5060190552936 33.0 10.0 35.0 8.0 36.0 49 25.178607805541898 32.0 10.0 35.0 8.0 36.0 50 24.707073816369856 31.0 10.0 35.0 8.0 35.0 51 24.402612812381573 31.0 10.0 35.0 8.0 35.0 52 23.760618754657813 30.0 9.0 35.0 8.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 5.0 11 17.0 12 18.0 13 35.0 14 47.0 15 123.0 16 342.0 17 1029.0 18 2696.0 19 5863.0 20 11065.0 21 20041.0 22 34105.0 23 52737.0 24 72527.0 25 97140.0 26 132150.0 27 164041.0 28 161902.0 29 124965.0 30 95268.0 31 96214.0 32 119691.0 33 150001.0 34 206847.0 35 273087.0 36 447050.0 37 402662.0 38 142963.0 39 24685.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 0.0 100.0 6 100.0 0.0 0.0 0.0 7 98.5500028880195 0.9219121788487087 0.34522399056674213 0.18286094256504032 8 95.01453871284492 3.31632688999745 0.8272062708060675 0.841928126351558 9 81.04289906442256 13.500258512965798 3.395465668491989 2.061376754119654 10 35.46660533734181 47.54338721015907 9.247825884825781 7.742181567673341 11 18.743880568418593 61.7474419895496 12.735813836853666 6.7728636051781494 12 41.06897576740314 38.39717030439726 13.50321697197494 7.030636956224669 13 18.222698706308137 39.68110629461462 23.94192826723056 18.154266731846686 14 27.90098037696403 49.42324137221782 9.11011666190026 13.565661588917893 15 21.630773045339087 46.229901849600395 9.528069436441735 22.611255668618778 16 21.236417503370532 53.481190540256875 18.53661233902813 6.745779617344459 17 35.872231199345194 39.1181890286252 16.58948845426152 8.420091317768081 18 22.651441403492953 40.62830625404147 24.357767856765502 12.362484485700078 19 27.163619688685586 37.451132596724 20.464013163733803 14.921234550856616 20 12.988515543884512 40.31735108033062 24.640089373637878 22.054044002147 21 12.793292469031273 41.79636926640078 34.251946595588514 11.15839166897943 22 19.007289079482522 43.43208012070513 24.787730566094087 12.772900233718262 23 20.10639182112875 54.10172731742434 17.78477633345496 8.007104527991952 24 27.490494189445624 41.36214496730903 18.78212921703678 12.365231626208566 25 20.49620401533327 37.11386826968185 23.46836350726724 18.921564207717633 26 30.384923692889416 36.51731614233851 11.233656274961998 21.864103889810078 27 37.76018590392897 37.44623705145887 11.522986522106029 13.270590522506126 28 36.05276763840305 43.358576502228004 9.502147700361636 11.08650815900731 29 35.81795756442749 44.531535059852445 10.68827844452678 8.962228931193287 30 25.70143654316744 54.03593682422104 11.072173720713018 9.1904529118985 31 20.823501153094618 56.67752374163355 11.373795660645028 11.125179444626806 32 16.97930064846604 58.54170511489387 15.203379968978444 9.275614267661648 33 19.69076355009446 57.861682179792595 12.786635936260705 9.660918333852237 34 16.87131689463237 57.94289892354356 12.048817391230845 13.136966790593227 35 17.423985213340114 60.62347410432653 12.119714748199918 9.832825934133432 36 20.82681180960485 56.28313297991488 11.76565060035586 11.12440461012441 37 19.663785221511095 54.61364638525617 14.599924770613768 11.12264362261897 38 22.554058794442042 51.772927000728345 13.934377152807226 11.738637052022389 39 20.984244092591315 49.738493355441946 14.707098470194936 14.570164081771807 40 22.16403528173687 48.195692201924686 16.759846385537923 12.880426130800519 41 21.393568028356125 48.59860614316969 17.24584371728966 12.761982111184524 42 21.415791690674794 50.99298563456832 14.9243691086163 12.666853566140576 43 24.026068250240552 48.876172993777374 14.762745675366883 12.33501308061519 44 21.756965409979024 49.347131492232634 16.089227123715713 12.806675974072629 45 21.36088410025513 48.57021902458198 14.64740099376047 15.421495881402421 46 22.725966394723233 48.415745200604654 14.851217687640261 14.00707071703185 47 23.060483581256893 47.605338750600495 14.793985593713415 14.540192074429193 48 23.055341497741004 47.137796567905795 15.105328184675464 14.70153374967774 49 22.05027548888535 47.02731221181439 15.629398066294838 15.293014233005414 50 19.529914951347436 47.86430957315071 16.855538446583616 15.750237028918232 51 18.83914999246297 47.51619756307505 17.7474433983396 15.897209046122377 52 20.13837135422757 44.82780359776791 18.61240524126233 16.421419806742186 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 332.0 6 664.0 7 1857.0 8 3050.0 9 17978.0 10 32906.0 11 44668.0 12 56430.0 13 36287.0 14 14845.5 15 13547.0 16 15344.0 17 17141.0 18 19472.0 19 21803.0 20 24712.0 21 27621.0 22 33197.0 23 38773.0 24 47155.0 25 55537.0 26 68178.5 27 80820.0 28 94715.0 29 108610.0 30 122857.0 31 137104.0 32 166064.0 33 195024.0 34 234193.5 35 273363.0 36 257266.0 37 241169.0 38 188838.0 39 124897.0 40 113287.0 41 112174.0 42 111061.0 43 109887.5 44 108714.0 45 104896.0 46 101078.0 47 89221.0 48 77364.0 49 74721.5 50 72079.0 51 77877.5 52 83676.0 53 98026.0 54 112376.0 55 134177.0 56 155978.0 57 150922.0 58 145866.0 59 134759.5 60 123653.0 61 103934.5 62 84216.0 63 62108.5 64 30676.0 65 21351.0 66 16693.0 67 12035.0 68 9682.5 69 7330.0 70 6546.5 71 5763.0 72 4441.0 73 3119.0 74 2747.5 75 2376.0 76 1768.5 77 1161.0 78 699.0 79 237.0 80 270.5 81 304.0 82 173.0 83 42.0 84 26.0 85 10.0 86 8.0 87 6.0 88 8.0 89 9.5 90 9.0 91 5.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 2839316.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.007339579399684 #Duplication Level Percentage of deduplicated Percentage of total 1 81.08684626667052 29.197216089465833 2 9.01507836947058 6.492179763688559 3 3.4804397609941646 3.7596412907928465 4 1.685671167951832 2.4278613665457955 5 0.9853213718466021 1.7739400615460275 6 0.6201214490029573 1.339735415683132 7 0.4436292307241184 1.118173585062183 8 0.3346206982740986 0.9639040890441049 9 0.2691011178463743 0.8720653798341407 >10 1.6816220888940803 11.711611636737945 >50 0.22091143089321952 5.608709415272871 >100 0.14984320999260228 10.364187002937667 >500 0.013396918719417675 3.385549712104153 >1k 0.011832315073354294 8.154635787196817 >5k 5.867263672737666E-4 1.3695224808092585 >10k+ 9.77877278789611E-4 11.461066923278901 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA 78222 2.754959293012824 No Hit TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAA 45535 1.6037313212055297 No Hit TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 43833 1.543787306520303 No Hit TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAA 39245 1.382199093020995 No Hit TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 27467 0.9673808762392069 No Hit TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA 25394 0.8943703342636043 No Hit TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAA 23098 0.8135057880137329 No Hit TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGAAG 16414 0.5780969782863197 No Hit TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAA 14757 0.5197378523559899 No Hit TAGTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA 11534 0.406224597755234 Illumina Single End Adapter 2 (97% over 34bp) TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAA 8089 0.284892558630318 No Hit TAGTCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA 7157 0.25206775152888933 Illumina Single End Adapter 2 (97% over 34bp) TAGTCGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA 7033 0.24770050251539452 Illumina Single End Adapter 2 (97% over 34bp) TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 6096 0.21469959666342175 No Hit TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAA 5476 0.19286335159594775 No Hit TAGTCGGGGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 5044 0.17764841954893362 No Hit TAGTCGGGGGAGTGCAGTGCTTAGTCGGGCAAAAAAAAAAAAAAAAAAAAAA 4953 0.17444342228903018 No Hit TAGTCGGGGGAGTGCAGTGCTTAGTCGGGATCGGAAGAGCTCGTATGCCGTC 4704 0.16567370451193175 Illumina Single End Adapter 2 (96% over 25bp) TAGTCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA 4695 0.16535672676095228 Illumina Single End Adapter 2 (97% over 34bp) TAGTCGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA 4084 0.14383745944445775 No Hit TAGTCGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA 4013 0.1413368571867309 Illumina Single End Adapter 2 (100% over 34bp) TAGTCGGGGGAGTGCAGTGCTTAGTCGGGTAAAAAAAAAAAAAAAAAAAAAA 3922 0.13813185992682744 No Hit TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAAAA 3887 0.13689916867301843 No Hit TAGTCGGGGGAGTGCAGTGCTTAGTCGGAAGAGCTCGTATGCCGTCTTCTGC 3788 0.13341241341224436 Illumina Single End Adapter 2 (96% over 29bp) TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAA 3733 0.13147532715625876 No Hit TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGCAAAAAAAAAAAAAAAAAAAAA 3612 0.12721373739309044 No Hit TAGTCGGGGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3596 0.12665022139134918 No Hit TAGTCGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 3435 0.12097984162382772 No Hit TAGTCGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA 3238 0.11404155085238839 Illumina Single End Adapter 2 (100% over 34bp) TAGTCGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAA 3219 0.11337237560032064 No Hit TAGTCGGGGGAGTGCAGTGCTTAGTCGAAAAAAAAAAAAAAAAAAAAAAAAA 3206 0.11291451884890585 No Hit TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3117 0.10977996108922007 No Hit TAGTCGGGAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA 3056 0.10763155633258151 No Hit TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAGTGCAGTGCTTAGTCGGGGA 2982 0.10502529482452816 No Hit TAGTCGGGGGAGTGCAGTGCTTGATCGGAAGAGCTCGTATGCCGTCTTCTGC 2881 0.10146810006353643 Illumina Single End Adapter 2 (96% over 31bp) TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2864 0.10086936431168633 No Hit TAGTCGGGGGAGTGCAGTGCTTAGTCGGATCGGAAGAGCTCGTATGCCGTCT 2846 0.10023540880972741 Illumina Single End Adapter 2 (96% over 26bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.08234377575444227 0.0 0.0 0.0 0.0 8 0.1310526901549528 0.0 0.0 0.0 0.0 9 0.22903403495771515 0.0 0.0 0.0 0.0 10 0.49120985476783846 0.0 0.0 0.0 0.0 11 0.8504865256280033 0.0 0.0 0.0 0.0 12 1.0201400619022327 0.0 0.0 0.0 0.0 13 1.1444305600362905 0.0 0.0 0.0 0.0 14 1.3955473783122414 0.0 0.0 0.0 0.0 15 1.6344781630505376 0.0 0.0 0.0 0.0 16 1.9123972111593073 0.0 0.0 0.0 0.0 17 2.090256949208894 0.0 0.0 0.0 0.0 18 2.2861139795640923 0.0 0.0 0.0 7.043950021765806E-5 19 2.4945796804582514 0.0 0.0 0.0 7.043950021765806E-5 20 2.7118150991295087 0.0 0.0 0.0 7.043950021765806E-5 21 2.93313600881339 0.0 0.0 0.0 7.043950021765806E-5 22 3.1730881663048423 0.0 0.0 0.0 7.043950021765806E-5 23 3.4376237093722573 0.0 0.0 0.0 7.043950021765806E-5 24 3.6803582271223068 0.0 0.0 0.0 7.043950021765806E-5 25 3.8930502980295256 0.0 0.0 0.0 7.043950021765806E-5 26 4.128142130005959 0.0 0.0 0.0 7.043950021765806E-5 27 4.316638232588412 0.0 0.0 0.0 7.043950021765806E-5 28 4.574834220636237 0.0 0.0 0.0 7.043950021765806E-5 29 4.7184251418299334 0.0 0.0 0.0 7.043950021765806E-5 30 4.9145991499361115 0.0 0.0 0.0 7.043950021765806E-5 31 5.077279175688792 0.0 0.0 0.0 1.0565925032648708E-4 32 5.244749087456275 0.0 0.0 0.0 1.0565925032648708E-4 33 5.393270773665207 0.0 0.0 0.0 1.0565925032648708E-4 34 5.547533279141878 0.0 0.0 0.0 1.0565925032648708E-4 35 5.800481524423488 0.0 0.0 0.0 1.0565925032648708E-4 36 5.971121213700765 0.0 0.0 0.0 1.0565925032648708E-4 37 6.155003529018961 0.0 0.0 0.0 1.0565925032648708E-4 38 6.343253093350652 0.0 0.0 0.0 1.4087900043531612E-4 39 6.557283514762006 0.0 0.0 0.0 2.1131850065297415E-4 40 6.7904734802325635 0.0 0.0 0.0 2.1131850065297415E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTCGCT 35 1.02028935E-7 46.000004 39 TCTAAGT 35 1.02028935E-7 46.000004 26 TAGACCG 45 3.110472E-10 46.0 41 GTAATAG 20 6.3130306E-4 46.0 18 GTAGCGA 25 3.4184333E-5 46.0 29 CATAAGT 25 3.4184333E-5 46.0 41 CGATGTA 25 3.4184333E-5 46.0 35 ACGAACT 50 8.731149E-10 41.4 24 TCACGCC 45 1.5897967E-8 40.88889 33 CACGGTT 45 1.5897967E-8 40.88889 19 AATAGCG 45 1.5897967E-8 40.88889 16 CGGCGTA 40 2.9126022E-7 40.25 13 TTAGCGA 40 2.9126022E-7 40.25 26 TCCCGAC 40 2.9126022E-7 40.25 44 ATACACG 40 2.9126022E-7 40.25 28 GAAGCAG 13135 0.0 40.046444 9 AAGCAGT 13205 0.0 39.834152 10 CGGGTAT 295 0.0 39.76271 5 GGAAGCA 13765 0.0 39.650562 8 TAATAGT 35 5.375905E-6 39.428574 37 >>END_MODULE