FastQCFastQC Report
Fri 17 Jun 2016
SRR1527664_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527664_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2862524
Sequences flagged as poor quality0
Sequence length52
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA748382.6144060276874534No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAA428631.4973848254198043No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410921.4355163485092177No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAA369841.292006634704198No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260900.9114334063225321No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA241170.8425082200184173No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAA220340.7697402711732723No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGAAG146430.5115415626209597No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAA140310.49016182921086426No Hit
TAGTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA112260.3921713844145936Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAA75380.2633340366753257No Hit
TAGTCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA70450.2461114736505266Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA68130.23800673810944467Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAA57450.20069700725653303No Hit
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAA52700.18410325992026616No Hit
TAGTCGGGGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA48640.1699199727233728No Hit
TAGTCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA46520.16251392128066Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGCAAAAAAAAAAAAAAAAAAAAAA46510.16247898707574154No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGATCGGAAGAGCTCGTATGCCGTC43840.15315155436251365Illumina Single End Adapter 2 (96% over 25bp)
TAGTCGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA39440.13778050419839274No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGTAAAAAAAAAAAAAAAAAAAAAA38680.13512550462459005No Hit
TAGTCGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA38140.1332390575589934Illumina Single End Adapter 2 (100% over 34bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGAAGAGCTCGTATGCCGTCTTCTGC36300.12681116385399738Illumina Single End Adapter 2 (96% over 29bp)
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGGAG36230.12656662441956817No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAAAA36190.12642688759989434No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGCAAAAAAAAAAAAAAAAAAAAA35310.12335267756707016No Hit
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAA35180.12289853290313024No Hit
TAGTCGGGGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA33770.11797281000962787No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA31890.11140517948495803No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAA31190.10895978514066608No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGAAAAAAAAAAAAAAAAAAAAAAAAA30860.10780695637835701No Hit
TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30590.10686373284555868No Hit
TAGTCGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA29710.1037895228127345Illumina Single End Adapter 2 (100% over 34bp)
TAGTCGGGAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA29680.10368472019797913No Hit
TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29250.1021825493864855No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAGTGCAGTGCTTAGTCGGGGA29220.10207774677173012No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTT206.313038E-446.00000423
TACGGCA206.313038E-446.00000428
CTAACGA206.313038E-446.00000420
TAACCGC206.313038E-446.00000425
AACGTCA253.418441E-546.046
ACACGCG301.8623832E-646.020
CACGCGC301.8623832E-646.021
CTAGCGT351.02028935E-745.99999623
CACGTGA601.8189894E-1242.16666841
CGCTGAT508.731149E-1041.420
CGACATA451.5897967E-840.8888924
TACACGT451.5897967E-840.8888929
ATTCTCG451.5897967E-840.8888919
GAAGCAG134350.040.453299
TAGCGTC402.9126022E-740.25000424
CGAGGGT800.040.2500045
TAACTAG402.9126022E-740.25000415
AAGCAGT136400.039.9464810
TACGAAC750.039.86666523
AGTACGA750.039.86666521
Produced by FastQC (version 0.11.5)