##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527664_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2862524
Sequences flagged as poor quality	0
Sequence length	52
%GC	41
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.571594858243984	31.0	31.0	33.0	30.0	34.0
2	31.65221042688201	31.0	31.0	34.0	30.0	34.0
3	32.176870831475995	33.0	31.0	34.0	30.0	34.0
4	35.16962652540206	35.0	35.0	37.0	33.0	37.0
5	35.693515582751445	37.0	35.0	37.0	35.0	37.0
6	35.80128935163513	37.0	35.0	37.0	35.0	37.0
7	36.41153157143835	37.0	37.0	37.0	35.0	37.0
8	36.44087839962215	37.0	37.0	37.0	35.0	37.0
9	37.93380072970567	39.0	39.0	39.0	35.0	39.0
10	37.14303565664428	39.0	37.0	39.0	34.0	39.0
11	36.6520860611125	39.0	37.0	39.0	32.0	39.0
12	36.87004545638744	39.0	37.0	39.0	33.0	39.0
13	36.05632127451158	38.0	35.0	39.0	30.0	39.0
14	37.5116732645735	39.0	37.0	40.0	33.0	40.0
15	37.77861949803739	40.0	37.0	40.0	33.0	40.0
16	37.62037907804441	40.0	37.0	40.0	33.0	40.0
17	37.814541642270946	40.0	38.0	40.0	33.0	40.0
18	37.65798889371757	40.0	37.0	40.0	33.0	40.0
19	37.64623632849891	40.0	37.0	40.0	33.0	40.0
20	37.85458008387004	40.0	38.0	40.0	33.0	40.0
21	37.950225395490136	40.0	38.0	40.0	33.0	40.0
22	37.55417456761935	39.0	37.0	40.0	33.0	40.0
23	36.735114185942194	38.0	36.0	40.0	32.0	40.0
24	36.19758402025625	37.0	35.0	40.0	31.0	40.0
25	35.77127528013739	37.0	35.0	40.0	30.0	40.0
26	35.65730593001142	37.0	35.0	40.0	30.0	40.0
27	35.47150626510031	37.0	35.0	40.0	30.0	40.0
28	35.17494910086344	37.0	35.0	40.0	28.0	40.0
29	34.76686064466185	36.0	35.0	40.0	25.0	40.0
30	34.1226525961005	36.0	33.0	40.0	23.0	40.0
31	33.59565718924976	35.0	33.0	39.0	21.0	40.0
32	33.226594082704636	35.0	33.0	39.0	18.0	40.0
33	32.78745889990791	35.0	32.0	39.0	17.0	40.0
34	32.226683514269226	35.0	31.0	39.0	15.0	40.0
35	31.61273757006055	35.0	31.0	38.0	11.0	40.0
36	30.621667451521805	35.0	29.0	38.0	10.0	40.0
37	29.632674520807512	35.0	24.0	37.0	9.0	40.0
38	28.739111357668968	35.0	21.0	36.0	8.0	39.0
39	28.221718665066213	34.0	20.0	35.0	8.0	39.0
40	27.741917971692114	34.0	18.0	35.0	8.0	39.0
41	27.24487654950666	34.0	15.0	35.0	8.0	38.0
42	26.991455792161045	33.0	15.0	35.0	8.0	38.0
43	26.79459456060456	33.0	15.0	35.0	8.0	38.0
44	26.451500493969657	33.0	13.0	35.0	8.0	38.0
45	26.273933423789636	33.0	12.0	35.0	8.0	37.0
46	25.943776191920136	33.0	10.0	35.0	8.0	37.0
47	25.70721153778973	33.0	10.0	35.0	8.0	36.0
48	25.43256894964025	33.0	10.0	35.0	8.0	36.0
49	25.087784067487295	32.0	10.0	35.0	8.0	35.0
50	24.501541995805102	31.0	10.0	35.0	8.0	35.0
51	24.156256506495666	30.0	10.0	35.0	8.0	35.0
52	23.653352076698745	29.0	9.0	35.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	11.0
12	20.0
13	24.0
14	46.0
15	122.0
16	385.0
17	1010.0
18	2703.0
19	5895.0
20	11414.0
21	20609.0
22	34660.0
23	53656.0
24	73622.0
25	100037.0
26	134239.0
27	164656.0
28	163195.0
29	125856.0
30	96975.0
31	99145.0
32	123910.0
33	156052.0
34	213081.0
35	286783.0
36	452090.0
37	386472.0
38	134786.0
39	21067.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	98.53716510324455	0.9335816922408335	0.34553422084845403	0.18371898366616313
8	95.04025119090704	3.2945749974498026	0.8241677624362277	0.8410060492069237
9	81.10255145459043	13.449424354171354	3.3899453768771894	2.0580788143610325
10	35.53685488750487	47.405785942755415	9.30947653189982	7.747882637839893
11	18.732698835014133	61.68161384847778	12.766600384835202	6.819086931672887
12	41.12866826618746	38.29571385253014	13.51667269863938	7.0589451826430105
13	18.57532024185649	39.403931635158344	23.893493993412807	18.12725412957236
14	27.90533808624836	49.39085226883687	9.123347088094283	13.580462556820486
15	21.69857789838618	46.10990161130527	9.55481945304214	22.636701037266413
16	21.20632001688021	53.398259717647775	18.615669248537305	6.779751016934705
17	35.97217001499376	39.00791748820272	16.589205889627475	8.430706607176045
18	22.70104984272621	40.53960770285245	24.37303582432846	12.386306630092884
19	27.164732941977082	37.396332746904484	20.505644668830726	14.933289642287715
20	12.997550413551117	40.250387420332544	24.66585432995496	22.08620783616137
21	12.793779196261761	41.692296728341844	34.31614896503925	11.197775110357153
22	19.23526230697105	43.216755562573454	24.760595893693818	12.787386236761684
23	20.15284413335923	54.036298036278474	17.750942874190752	8.059914956171546
24	27.510965846923902	41.33995033753429	18.77430547307202	12.374778342469792
25	20.551338608864064	37.03581175214601	23.492833597203028	18.920016041786898
26	30.510696154861932	36.420026522048374	11.235399249054332	21.83387807403536
27	37.83147320336877	37.379564328543616	11.495903615131262	13.29305885295634
28	36.05999460615876	43.308422916279476	9.548880638206004	11.082701839355757
29	35.84207503587743	44.45129543018679	10.725814001908805	8.98081553202698
30	25.741967578263097	53.929818579687016	11.09646591609363	9.231747925956254
31	20.830882116621556	56.60434637403914	11.403293037892432	11.161478471446877
32	17.02906246375576	58.406462268962635	15.255872090504743	9.308603176776858
33	19.701703811042286	57.577892796706685	12.929882858624067	9.790520533626967
34	16.90012031340174	57.82941907211957	12.10484174106488	13.165618873413813
35	17.493722323376154	60.50660186604549	12.131880815671764	9.867794994906593
36	21.09830345527234	55.93591529712939	11.806154289012074	11.159626958586198
37	19.722070452509744	54.525935852415564	14.616017193218292	11.135976501856405
38	22.69291017298021	51.307552355892916	13.891342046389829	12.108195424737051
39	20.99213840652515	49.722377873513025	14.709536059785	14.575947660176821
40	22.193840121515137	48.17280134594505	16.790741317802052	12.84261721473776
41	21.71188783046011	48.20232074910114	17.30958412925097	12.776207291187777
42	21.416833535718826	50.963869647905135	14.949988192238736	12.6693086241373
43	24.03109982658661	48.825896306895594	14.791212230884351	12.351791635633449
44	21.829231824781207	49.251255185982714	16.09380392967884	12.82570905955723
45	21.406737550497393	48.49594274144077	14.63645370309559	15.460866004966247
46	22.925292504097783	48.20525522231429	14.919874907599029	13.949577365988898
47	23.33367335959454	47.375602789705866	14.840923604483317	14.449800246216277
48	23.072575112033995	47.090644480186015	15.153654606913339	14.683125800866648
49	22.342589966057925	46.906855628110016	15.493040407696146	15.257513998135911
50	19.840672078207902	47.785381013399366	16.6729082446121	15.701038663780636
51	18.903142820811283	47.418012914476876	17.72603478608389	15.952809478627952
52	20.240109777245536	44.75134531623141	18.55404531106115	16.454499595461908
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	337.0
6	674.0
7	1787.5
8	2901.0
9	17441.5
10	31982.0
11	43642.0
12	55302.0
13	36671.5
14	16048.5
15	14056.0
16	15475.0
17	16894.0
18	19300.0
19	21706.0
20	24566.0
21	27426.0
22	32732.5
23	38039.0
24	46654.0
25	55269.0
26	67804.5
27	80340.0
28	94322.0
29	108304.0
30	122664.0
31	137024.0
32	166155.5
33	195287.0
34	233508.0
35	271729.0
36	258022.0
37	244315.0
38	193138.5
39	128406.0
40	114850.0
41	113570.5
42	112291.0
43	111023.5
44	109756.0
45	105716.5
46	101677.0
47	90249.5
48	78822.0
49	75770.0
50	72718.0
51	78771.5
52	84825.0
53	99125.0
54	113425.0
55	134633.0
56	155841.0
57	152271.0
58	148701.0
59	137369.0
60	126037.0
61	106118.0
62	86199.0
63	63657.5
64	31457.0
65	21798.0
66	17099.5
67	12401.0
68	10012.0
69	7623.0
70	6754.5
71	5886.0
72	4507.5
73	3129.0
74	2775.5
75	2422.0
76	1778.5
77	1135.0
78	688.0
79	241.0
80	266.5
81	292.0
82	165.0
83	38.0
84	26.0
85	14.0
86	13.5
87	13.0
88	12.0
89	11.0
90	11.0
91	5.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2862524.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.77832528102743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.1696973335106	29.852855314943977
2	9.001420875178763	6.621143698775104
3	3.472698774772679	3.8316013542484506
4	1.6952887751944794	2.4939952807750863
5	0.9572094295350848	1.7602279880754028
6	0.6085348273145201	1.3428535094284366
7	0.4316110526610274	1.1111752182757748
8	0.33439668828460956	0.9838840139703764
9	0.25535326299491407	0.8452318831198825
>10	1.6914954100728758	11.909522907691317
>50	0.21125918670701466	5.4885412116309915
>100	0.14496570838536438	10.240100990713477
>500	0.013812507108590756	3.5763977402988636
>1k	0.010797100627137842	7.7672976711060135
>5k	4.863558841053081E-4	1.1594941198442175
>10k+	9.727117682106162E-4	11.015677097102582
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA	74838	2.6144060276874534	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAA	42863	1.4973848254198043	No Hit
TAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41092	1.4355163485092177	No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAA	36984	1.292006634704198	No Hit
TAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26090	0.9114334063225321	No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA	24117	0.8425082200184173	No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAA	22034	0.7697402711732723	No Hit
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGAAG	14643	0.5115415626209597	No Hit
TAGTCGGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAA	14031	0.49016182921086426	No Hit
TAGTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	11226	0.3921713844145936	Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAA	7538	0.2633340366753257	No Hit
TAGTCGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	7045	0.2461114736505266	Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	6813	0.23800673810944467	Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	5745	0.20069700725653303	No Hit
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAA	5270	0.18410325992026616	No Hit
TAGTCGGGGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	4864	0.1699199727233728	No Hit
TAGTCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	4652	0.16251392128066	Illumina Single End Adapter 2 (97% over 34bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGCAAAAAAAAAAAAAAAAAAAAAA	4651	0.16247898707574154	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGATCGGAAGAGCTCGTATGCCGTC	4384	0.15315155436251365	Illumina Single End Adapter 2 (96% over 25bp)
TAGTCGGGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAA	3944	0.13778050419839274	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGTAAAAAAAAAAAAAAAAAAAAAA	3868	0.13512550462459005	No Hit
TAGTCGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	3814	0.1332390575589934	Illumina Single End Adapter 2 (100% over 34bp)
TAGTCGGGGGAGTGCAGTGCTTAGTCGGAAGAGCTCGTATGCCGTCTTCTGC	3630	0.12681116385399738	Illumina Single End Adapter 2 (96% over 29bp)
TAGTCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGGAG	3623	0.12656662441956817	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAAAAAAAAAAAAAAAAAAAAA	3619	0.12642688759989434	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGCAAAAAAAAAAAAAAAAAAAAA	3531	0.12335267756707016	No Hit
TAGTCGGGGCAGAGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAA	3518	0.12289853290313024	No Hit
TAGTCGGGGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3377	0.11797281000962787	No Hit
TAGTCGGGGTGCAGTGCTTAGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	3189	0.11140517948495803	No Hit
TAGTCGGGGAGTGCAGTGCTTAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAA	3119	0.10895978514066608	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGAAAAAAAAAAAAAAAAAAAAAAAAA	3086	0.10780695637835701	No Hit
TAGTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3059	0.10686373284555868	No Hit
TAGTCGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	2971	0.1037895228127345	Illumina Single End Adapter 2 (100% over 34bp)
TAGTCGGGAGAGTGCAGTGCTTAGTCGGGGAAAAAAAAAAAAAAAAAAAAAA	2968	0.10368472019797913	No Hit
TAGTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2925	0.1021825493864855	No Hit
TAGTCGGGGGAGTGCAGTGCTTAGTCGGGGGAGTGCAGTGCTTAGTCGGGGA	2922	0.10207774677173012	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.08370235498462196	0.0	0.0	0.0	0.0
8	0.12803386102614336	0.0	0.0	0.0	3.493420491845658E-5
9	0.22295009578958988	0.0	0.0	0.0	3.493420491845658E-5
10	0.4763977524729924	0.0	0.0	0.0	3.493420491845658E-5
11	0.8269974330346226	0.0	0.0	0.0	3.493420491845658E-5
12	0.9973016820120985	0.0	0.0	0.0	3.493420491845658E-5
13	1.1155190314561554	0.0	0.0	0.0	3.493420491845658E-5
14	1.3715867535084423	0.0	0.0	0.0	3.493420491845658E-5
15	1.6110956624293804	0.0	0.0	0.0	3.493420491845658E-5
16	1.8920714725885268	0.0	0.0	0.0	3.493420491845658E-5
17	2.0761397983038745	0.0	0.0	0.0	3.493420491845658E-5
18	2.266251741470115	0.0	0.0	0.0	1.0480261475536974E-4
19	2.4708264454725968	0.0	0.0	0.0	1.0480261475536974E-4
20	2.6808159512374394	0.0	0.0	0.0	1.0480261475536974E-4
21	2.903032428723742	0.0	0.0	0.0	1.0480261475536974E-4
22	3.135100351997049	0.0	0.0	0.0	1.0480261475536974E-4
23	3.4021374143937306	0.0	0.0	0.0	1.0480261475536974E-4
24	3.6376288897490467	0.0	0.0	0.0	1.0480261475536974E-4
25	3.8477930665384816	0.0	0.0	0.0	1.0480261475536974E-4
26	4.072944017237934	0.0	0.0	0.0	1.0480261475536974E-4
27	4.260505763445128	0.0	0.0	0.0	1.0480261475536974E-4
28	4.515839867194127	0.0	0.0	0.0	1.0480261475536974E-4
29	4.66787352699925	0.0	0.0	0.0	1.0480261475536974E-4
30	4.860291127690108	0.0	0.0	0.0	1.0480261475536974E-4
31	5.022176233282236	0.0	0.0	0.0	1.746710245922829E-4
32	5.189336403817051	0.0	0.0	0.0	1.746710245922829E-4
33	5.338819866663127	0.0	0.0	0.0	1.746710245922829E-4
34	5.489770566115777	0.0	0.0	0.0	1.746710245922829E-4
35	5.735288158282691	0.0	0.0	0.0	1.746710245922829E-4
36	5.905382802030656	0.0	0.0	0.0	1.746710245922829E-4
37	6.083302707680355	0.0	0.0	0.0	1.746710245922829E-4
38	6.274532545403986	0.0	0.0	0.0	2.4453943442919606E-4
39	6.479386723045815	0.0	0.0	0.0	2.4453943442919606E-4
40	6.710406620171569	0.0	0.0	0.0	2.4453943442919606E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGTT	20	6.313038E-4	46.000004	23
TACGGCA	20	6.313038E-4	46.000004	28
CTAACGA	20	6.313038E-4	46.000004	20
TAACCGC	20	6.313038E-4	46.000004	25
AACGTCA	25	3.418441E-5	46.0	46
ACACGCG	30	1.8623832E-6	46.0	20
CACGCGC	30	1.8623832E-6	46.0	21
CTAGCGT	35	1.02028935E-7	45.999996	23
CACGTGA	60	1.8189894E-12	42.166668	41
CGCTGAT	50	8.731149E-10	41.4	20
CGACATA	45	1.5897967E-8	40.88889	24
TACACGT	45	1.5897967E-8	40.88889	29
ATTCTCG	45	1.5897967E-8	40.88889	19
GAAGCAG	13435	0.0	40.45329	9
TAGCGTC	40	2.9126022E-7	40.250004	24
CGAGGGT	80	0.0	40.250004	5
TAACTAG	40	2.9126022E-7	40.250004	15
AAGCAGT	13640	0.0	39.94648	10
TACGAAC	75	0.0	39.866665	23
AGTACGA	75	0.0	39.866665	21
>>END_MODULE