FastQCFastQC Report
Fri 17 Jun 2016
SRR1527663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2331636
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC493272.11555319955602No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG266791.1442180511880928No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT99440.4264816635186624No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC98630.42300770789265557No Hit
TCGTAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG92540.39688870818601185No Hit
TCGTAAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA78320.3359014871961147No Hit
TCGTAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA69560.298331300425967No Hit
TCGTAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG51880.2225047134286827No Hit
TCGTAAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT45900.1968574854737189No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT43310.18574940513870947No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC41040.17601375171767805No Hit
TCGTAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT36640.1571428816504806No Hit
TCGTAAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAA36430.15624222648818253No Hit
TCGTAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT35440.15199628072306312No Hit
TCGTAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT34720.14890832016661262No Hit
TCGTAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC34370.14740722822944918No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC34050.1460348013154712No Hit
TCGTAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT33310.14286106407689708No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACTGACTGC31550.1353127160500181No Hit
TCGTAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC29100.12480507248987405No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTG27530.11807160294316953No Hit
TCGTAAGGGGGCTGTGACAGCCACACACCCCAAGGCCTCCAAGATGAGCT26300.1127963369925666No Hit
TCGTAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC24820.10644886251541835No Hit
TCGTAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT24380.10456177550869862No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGC24140.1035324553232151No Hit
TCGTAAGGGGCCGGGCAGTGGTGGCACACGCCTTTAATCTCAGCACTTGA24000.10293201854834975No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGC23700.10164536831649537No Hit
TCGTAAGGGGAGCATTCGCAGACTGCAGCTCCCAGACACCGCCCGCATCC23340.10010138803827012No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG207.8581483E-444.00000424
AACCGTT254.444253E-544.029
ATTCACG454.802132E-1044.033
TCGTAAG2402050.042.5382461
CGTAAGG2393200.042.534682
CGATGCG1500.042.53333322
GTAAGGG2385250.042.4855233
TAAGGGG1726050.042.4424554
TAAGGGA433700.042.381834
AAGGGGG848850.042.2868585
AGGGGGG417350.041.9652566
AAGGGAT124100.041.748595
AAGGGGT183250.041.694955
AAGGGGA457650.041.5098885
AAGGGGC259850.041.2738155
TAAGGGT78300.041.1340984
AGGGGGT132050.041.1177566
AAGGGAG161300.041.1084945
CGTAAAG8200.041.048782
AGGGGGC200950.041.0330966
Produced by FastQC (version 0.11.5)