##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527663_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2331636
Sequences flagged as poor quality	0
Sequence length	50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43428476829145	33.0	31.0	34.0	31.0	34.0
2	32.863752318114834	34.0	31.0	34.0	31.0	34.0
3	33.09582027383348	34.0	31.0	34.0	31.0	34.0
4	35.55800862570315	35.0	35.0	37.0	35.0	37.0
5	36.17740504950172	37.0	35.0	37.0	35.0	37.0
6	36.46651321218235	37.0	37.0	37.0	35.0	37.0
7	36.52014208049627	37.0	37.0	37.0	35.0	37.0
8	36.67251534973727	37.0	37.0	37.0	35.0	37.0
9	38.770708206598286	39.0	39.0	39.0	39.0	39.0
10	38.312573231842364	39.0	39.0	39.0	37.0	39.0
11	38.01799208795884	39.0	38.0	39.0	37.0	39.0
12	37.21659470003036	39.0	35.0	39.0	35.0	39.0
13	37.03637059987065	39.0	35.0	39.0	34.0	39.0
14	37.674795722831526	40.0	36.0	40.0	33.0	40.0
15	37.77445021435593	40.0	36.0	40.0	33.0	40.0
16	37.85309799642826	40.0	36.0	40.0	34.0	40.0
17	37.59597681627836	40.0	36.0	40.0	33.0	40.0
18	37.54805810169341	40.0	36.0	40.0	33.0	40.0
19	37.48944346373104	40.0	36.0	40.0	33.0	40.0
20	37.38401620149972	40.0	35.0	40.0	33.0	40.0
21	37.3884019632567	40.0	35.0	40.0	33.0	40.0
22	37.55855116321759	40.0	35.0	40.0	33.0	40.0
23	37.591139869173404	40.0	35.0	40.0	34.0	40.0
24	37.57542986984246	40.0	35.0	40.0	34.0	40.0
25	37.55608980132405	40.0	35.0	40.0	34.0	40.0
26	37.47573248997699	40.0	35.0	40.0	34.0	40.0
27	37.36985061133041	40.0	35.0	40.0	34.0	40.0
28	37.19127728341817	39.0	35.0	40.0	33.0	40.0
29	37.28200370898374	40.0	35.0	40.0	33.0	40.0
30	37.36415160857012	40.0	35.0	40.0	33.0	40.0
31	37.3856227987559	40.0	35.0	40.0	33.0	40.0
32	37.40014993764035	40.0	35.0	40.0	34.0	40.0
33	37.33004251092366	40.0	35.0	40.0	33.0	40.0
34	36.88219258923777	40.0	35.0	40.0	32.0	40.0
35	36.862725142346406	40.0	35.0	40.0	32.0	40.0
36	36.89913734390788	39.0	35.0	40.0	33.0	40.0
37	36.85455319784049	39.0	35.0	40.0	33.0	40.0
38	36.749122933425284	39.0	35.0	40.0	33.0	40.0
39	36.62930234393362	39.0	35.0	40.0	32.0	40.0
40	36.419029814259176	39.0	35.0	40.0	32.0	40.0
41	36.21028325175971	38.0	35.0	40.0	31.0	40.0
42	36.133332990226606	38.0	35.0	40.0	31.0	40.0
43	36.03800121459782	38.0	35.0	40.0	31.0	40.0
44	35.71457294363271	37.0	35.0	40.0	30.0	40.0
45	35.66786925575004	37.0	35.0	40.0	30.0	40.0
46	35.51827129105915	37.0	35.0	40.0	30.0	40.0
47	35.42347733522728	36.0	35.0	40.0	30.0	40.0
48	35.32616969372578	36.0	35.0	40.0	30.0	40.0
49	35.248624142018734	36.0	35.0	40.0	30.0	40.0
50	34.54701462835537	35.0	34.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	3.0
14	13.0
15	36.0
16	82.0
17	155.0
18	359.0
19	574.0
20	1045.0
21	1618.0
22	2505.0
23	3793.0
24	5716.0
25	7772.0
26	10266.0
27	13820.0
28	18335.0
29	24796.0
30	34605.0
31	47406.0
32	61405.0
33	85442.0
34	212211.0
35	216454.0
36	182507.0
37	335611.0
38	692429.0
39	372676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.6082578927414	0.3429351751302519	0.03512555132962435	0.013681380798718154
8	99.6699313271883	0.2803610855210676	0.02762009164380718	0.02208749564683338
9	99.53114465551226	0.32556539700021786	0.06373207481785322	0.079557872669662
10	72.2649247138061	18.12208252059927	3.273709961589202	6.339282804005427
11	43.55581231375738	25.800425109236603	15.099784014314412	15.543978562691604
12	37.9323359220736	14.204103899579524	23.136587357546375	24.7269728208005
13	27.160457292647738	15.80967183556953	26.632416037494703	30.39745483428803
14	25.29146916585608	16.476585539080716	31.385945319080683	26.845999975982526
15	28.085215702622534	17.872601040642706	28.804367405547005	25.23781585118775
16	34.33593408233532	17.97656237937654	24.196058046796328	23.49144549149181
17	33.269901476902916	19.171002677948014	23.74367182527633	23.81542401987274
18	32.93592996505458	18.225829417627796	24.104148331900863	24.734092285416764
19	30.834186811320464	22.36403966999995	22.019002966157668	24.78277055252192
20	32.277551041414696	22.016558330717146	22.16936948992038	23.536521137947773
21	30.750254327862496	22.392817746852426	23.422952810816096	23.433975114468982
22	28.667810927606197	20.574780969242198	27.43459956871484	23.322808534436763
23	31.9742018050845	18.58875055969285	25.215213695448174	24.221833939774477
24	33.59894082952914	18.755371764717992	22.54820220651937	25.0974851992335
25	29.923838883942434	20.495308873254658	21.229299942186515	28.35155230061639
26	26.738092909871007	19.575782840889403	25.224391800435402	28.461732448804188
27	26.494444244298855	18.226172524356286	24.271026866972374	31.008356364372485
28	27.035437778452554	22.451488997424985	21.269786536148867	29.243286687973598
29	32.31825207708236	21.039433256305873	20.52820423084907	26.1141104357627
30	30.357783118805852	18.48200147879	24.810090425778295	26.350124976625857
31	33.931196807735	18.19387760353674	23.059688562022547	24.81523702670571
32	35.68914701951763	18.593082282140095	22.164437330698274	23.553333367644004
33	35.83938487825716	18.43675427896979	22.22495277993649	23.498908062836566
34	30.738460034070496	19.37034768720332	28.09568045784162	21.795511820884563
35	28.4407171616839	25.82632966723794	24.748288326308224	20.984664844769938
36	29.034592020366816	27.626524894966455	21.523085078459932	21.8157980062068
37	31.663261332386362	25.191110447771436	19.441670998389114	23.70395722145309
38	30.39020670464858	26.050163919239537	20.519026125861842	23.04060325025004
39	32.991942138481306	22.57423542954389	20.22798584341638	24.20583658855842
40	30.658687719695525	21.040505464832417	20.341382617183815	27.95942419828824
41	25.976953521047026	24.465954377098313	21.813267594084156	27.74382450777051
42	25.22520667891558	22.205395696412307	21.915427622493393	30.653970002178728
43	25.63161659881731	19.346373104549766	23.969521829307833	31.05248846732509
44	26.480462645112702	18.410206395852526	23.876840124273258	31.232490834761517
45	29.71312846430575	20.1948331557756	22.412932378810417	27.679106001108234
46	27.48117630710797	23.99499750389855	21.819229073491744	26.704597115501734
47	25.877366793101498	21.600584310758624	24.141632741988886	28.38041615415099
48	27.72606873457092	20.38714447709677	26.18637729045186	25.70040949788046
49	30.807252933133643	19.766593070273405	23.46734224381507	25.958811752777876
50	26.619935530245716	20.79231063510771	23.98551918052389	28.602234654122682
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	5.5
4	11.0
5	17.5
6	24.0
7	16.0
8	8.0
9	114.0
10	220.0
11	1354.0
12	2488.0
13	3632.5
14	4777.0
15	4809.0
16	4841.0
17	4584.0
18	4327.0
19	4775.0
20	5223.0
21	4954.0
22	4685.0
23	4987.0
24	5289.0
25	6146.5
26	7004.0
27	8181.5
28	9359.0
29	10982.0
30	12605.0
31	14168.5
32	15732.0
33	20406.0
34	25080.0
35	26978.0
36	28876.0
37	31066.0
38	33256.0
39	36231.5
40	39207.0
41	42948.0
42	46689.0
43	50290.0
44	53891.0
45	60267.5
46	66644.0
47	75283.5
48	83923.0
49	90707.0
50	97491.0
51	108553.0
52	119615.0
53	130794.0
54	141973.0
55	161849.0
56	181725.0
57	209060.5
58	236396.0
59	246146.0
60	255896.0
61	271466.0
62	287036.0
63	237391.5
64	187747.0
65	154656.0
66	121565.0
67	101658.5
68	81752.0
69	74006.0
70	66260.0
71	55982.0
72	45704.0
73	37012.0
74	28320.0
75	22225.5
76	16131.0
77	10824.5
78	5518.0
79	4273.0
80	3028.0
81	2030.0
82	1032.0
83	622.0
84	212.0
85	128.0
86	44.0
87	34.5
88	25.0
89	14.5
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2331636.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.665563013983995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.202701594834664	4.844842917292318
2	10.231679563082405	1.5686316885940084
3	4.049536581233815	0.9312593352264337
4	2.013077576084779	0.6172549204606412
5	1.1823226792327952	0.45315845002606886
6	0.7847747754859512	0.3609444295963621
7	0.6025459148154787	0.3233197575185679
8	0.5150881025931099	0.3158752246544749
9	0.41501429517575544	0.28631864082365166
>10	10.305374972011764	22.61533217978304
>50	4.421148593804951	23.30484351778849
>100	2.1444132421575683	28.02275364179106
>500	0.0800747529513287	4.171699112932149
>1k	0.047704108141217096	6.740244747415
>5k	0.003407436295801221	2.134737335651937
>10k+	0.0011358120986004069	3.3087841004458087
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	49327	2.11555319955602	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	26679	1.1442180511880928	No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	9944	0.4264816635186624	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	9863	0.42300770789265557	No Hit
TCGTAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	9254	0.39688870818601185	No Hit
TCGTAAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA	7832	0.3359014871961147	No Hit
TCGTAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	6956	0.298331300425967	No Hit
TCGTAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	5188	0.2225047134286827	No Hit
TCGTAAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	4590	0.1968574854737189	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	4331	0.18574940513870947	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC	4104	0.17601375171767805	No Hit
TCGTAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	3664	0.1571428816504806	No Hit
TCGTAAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAA	3643	0.15624222648818253	No Hit
TCGTAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	3544	0.15199628072306312	No Hit
TCGTAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3472	0.14890832016661262	No Hit
TCGTAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	3437	0.14740722822944918	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	3405	0.1460348013154712	No Hit
TCGTAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3331	0.14286106407689708	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACTGACTGC	3155	0.1353127160500181	No Hit
TCGTAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	2910	0.12480507248987405	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTG	2753	0.11807160294316953	No Hit
TCGTAAGGGGGCTGTGACAGCCACACACCCCAAGGCCTCCAAGATGAGCT	2630	0.1127963369925666	No Hit
TCGTAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	2482	0.10644886251541835	No Hit
TCGTAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	2438	0.10456177550869862	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGC	2414	0.1035324553232151	No Hit
TCGTAAGGGGCCGGGCAGTGGTGGCACACGCCTTTAATCTCAGCACTTGA	2400	0.10293201854834975	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGC	2370	0.10164536831649537	No Hit
TCGTAAGGGGAGCATTCGCAGACTGCAGCTCCCAGACACCGCCCGCATCC	2334	0.10010138803827012	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.018270433292332078	0.0	0.0	0.0	0.0
7	0.018356209974455705	0.0	0.0	0.0	0.0
8	0.02817764007761074	0.0	0.0	0.0	0.0
9	0.06133032771839172	0.0	0.0	0.0	0.0
10	0.07591236367940793	0.0	0.0	0.0	0.0
11	0.10713507597240736	0.0	0.0	0.0	0.0
12	0.11991580160882745	0.0	0.0	0.0	0.0
13	0.1311096586259605	0.0	0.0	0.0	0.0
14	0.14140286048079545	0.0	0.0	0.0	0.0
15	0.15083829551439418	0.0	0.0	0.0	0.0
16	0.16211792921365084	0.0	0.0	0.0	0.0
17	0.17193935931680587	0.0	0.0	0.0	0.0
18	0.1810316876219101	0.0	0.0	0.0	0.0
19	0.19424129666894832	0.0	0.0	0.0	0.0
20	0.20715068732855385	0.0	0.0	0.0	0.0
21	0.21658612236215258	0.0	0.0	0.0	0.0
22	0.22422024707115518	0.0	0.0	0.0	0.0
23	0.23314102201201217	0.0	0.0	0.0	0.0
24	0.2433055588436617	0.0	0.0	0.0	0.0
25	0.25179745037390056	0.0	0.0	0.0	0.0
26	0.2605895602915721	0.0	0.0	0.0	0.0
27	0.2700249953251708	0.0	0.0	0.0	0.0
28	0.2785597751964715	0.0	0.0	0.0	0.0
29	0.2886385353459974	0.0	0.0	0.0	0.0
30	0.29880307217764696	0.0	0.0	0.0	0.0
31	0.30780962380062754	0.0	0.0	0.0	0.0
32	0.3183172673607716	0.0	0.0	0.0	0.0
33	0.32693782391419585	0.0	0.0	0.0	0.0
34	0.33701658406372176	0.0	0.0	0.0	0.0
35	0.34941131463058556	0.0	0.0	0.0	0.0
36	0.3635644671809837	0.0	0.0	0.0	0.0
37	0.37467254751599305	0.0	0.0	0.0	0.0
38	0.38552329780463157	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	20	7.8581483E-4	44.000004	24
AACCGTT	25	4.444253E-5	44.0	29
ATTCACG	45	4.802132E-10	44.0	33
TCGTAAG	240205	0.0	42.538246	1
CGTAAGG	239320	0.0	42.53468	2
CGATGCG	150	0.0	42.533333	22
GTAAGGG	238525	0.0	42.485523	3
TAAGGGG	172605	0.0	42.442455	4
TAAGGGA	43370	0.0	42.38183	4
AAGGGGG	84885	0.0	42.286858	5
AGGGGGG	41735	0.0	41.965256	6
AAGGGAT	12410	0.0	41.74859	5
AAGGGGT	18325	0.0	41.69495	5
AAGGGGA	45765	0.0	41.509888	5
AAGGGGC	25985	0.0	41.273815	5
TAAGGGT	7830	0.0	41.134098	4
AGGGGGT	13205	0.0	41.117756	6
AAGGGAG	16130	0.0	41.108494	5
CGTAAAG	820	0.0	41.04878	2
AGGGGGC	20095	0.0	41.033096	6
>>END_MODULE