FastQCFastQC Report
Fri 17 Jun 2016
SRR1527662_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527662_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3498062
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC730212.0874701477561004No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT390891.1174473179720656No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA150860.4312673703324869No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC144330.4125998910253735No Hit
TCGTAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG141830.4054530765892657No Hit
TCGTAAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC119590.341875015365651No Hit
TCGTAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC109540.3131448213324978No Hit
TCGTAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG79570.22746880987243792No Hit
TCGTAAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT69430.19848133051958483No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC63760.18227235537849246No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC62080.17746969607742802No Hit
TCGTAAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA56740.16220410044190184No Hit
TCGTAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC55820.15957407272941418No Hit
TCGTAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT55410.15840199516189252No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC50580.14459434967133228No Hit
TCGTAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT50550.144508587898099No Hit
TCGTAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG50510.14439423886712127No Hit
TCGTAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT48370.13827656570981303No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC46560.13310227205807101No Hit
TCGTAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG43320.12384000054887535No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT42330.12100986203217667No Hit
TCGTAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT38560.11023246586252615No Hit
TCGTAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG37650.10763102540778294No Hit
TCGTAAGGGGCCGGGCAGTGGTGGCACACGCCTTTAATCTCAGCACTTGAGA37450.10705928025289432No Hit
TCGTAAGGGGGCTGTGACAGCCACACACCCCAAGGCCTCCAAGATGAGCTAC36950.10562991736567276No Hit
TCGTAAGGGGGTTCTTCTGCGCTGCGCCAGGAGCTGCAAGCACAGGCATCCA35630.10185639934340786No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTC35000.1000554021055087No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGC206.3132326E-446.023
TCGTTAC206.3132326E-446.023
TTATACG206.3132326E-446.031
TACGCTA453.110472E-1046.037
CGTAAGG3594750.044.493862
TCGTAAG3604750.044.4922941
GTAAGGG3583600.044.444893
TAAGGGG2596850.044.4217034
AAGGGGG1267200.044.246695
TAAGGGA653150.044.161834
AGGGGGG627300.043.8110966
AAGGGAT186650.043.7326555
AAGGGGT270850.043.528895
AAGGGGA702950.043.428265
AAGGGGC392350.043.2506685
TAAGGGT121900.043.1509444
AGGGGGC293450.043.1235286
AGGGGAG355850.043.0009846
AGGGGGT198150.042.9588746
AAGGGAG241050.042.803575
Produced by FastQC (version 0.11.5)