##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527662_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3498062
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16410486720933	33.0	31.0	34.0	31.0	34.0
2	32.59781044475484	34.0	31.0	34.0	31.0	34.0
3	32.8681515650666	34.0	31.0	34.0	31.0	34.0
4	35.54376394700837	37.0	35.0	37.0	35.0	37.0
5	35.966039767162506	37.0	35.0	37.0	35.0	37.0
6	36.305837346507865	37.0	37.0	37.0	35.0	37.0
7	36.41412730820666	37.0	36.0	37.0	35.0	37.0
8	36.583290976546444	37.0	37.0	37.0	35.0	37.0
9	38.68670538143692	39.0	39.0	39.0	38.0	39.0
10	38.1045130132056	39.0	38.0	39.0	37.0	39.0
11	37.73535774951959	39.0	38.0	39.0	35.0	39.0
12	36.91810922733788	39.0	35.0	39.0	34.0	39.0
13	36.73106823149504	39.0	35.0	39.0	33.0	39.0
14	37.391222625556665	40.0	36.0	40.0	33.0	40.0
15	37.520719472668006	40.0	36.0	40.0	33.0	40.0
16	37.60438236943771	40.0	36.0	40.0	33.0	40.0
17	37.41166823229548	40.0	35.0	40.0	33.0	40.0
18	37.43317070995311	40.0	35.0	40.0	33.0	40.0
19	37.410414966915965	40.0	35.0	40.0	33.0	40.0
20	37.38323820446865	40.0	35.0	40.0	33.0	40.0
21	37.36497351962315	40.0	35.0	40.0	33.0	40.0
22	37.38085517066307	40.0	35.0	40.0	33.0	40.0
23	37.405321575203644	40.0	35.0	40.0	33.0	40.0
24	37.36987051687477	40.0	35.0	40.0	33.0	40.0
25	37.35203921485668	40.0	35.0	40.0	33.0	40.0
26	37.23624424038225	39.0	35.0	40.0	33.0	40.0
27	37.1449939995346	39.0	35.0	40.0	33.0	40.0
28	37.00187646759834	39.0	35.0	40.0	33.0	40.0
29	36.94353988008217	39.0	35.0	40.0	33.0	40.0
30	36.754500634923	39.0	35.0	40.0	32.0	40.0
31	36.63453077732756	39.0	35.0	40.0	31.0	40.0
32	36.58125413443215	38.0	35.0	40.0	31.0	40.0
33	36.49809751799711	38.0	35.0	40.0	31.0	40.0
34	36.098499969411634	38.0	35.0	40.0	30.0	40.0
35	36.00937604879502	38.0	35.0	40.0	30.0	40.0
36	36.121992120208276	38.0	35.0	40.0	31.0	40.0
37	36.10511391736338	38.0	35.0	40.0	31.0	40.0
38	35.990313779458454	38.0	35.0	40.0	30.0	40.0
39	35.88717895794871	38.0	35.0	40.0	30.0	40.0
40	35.63756274188393	37.0	34.0	40.0	30.0	40.0
41	35.42523603069357	37.0	34.0	40.0	29.0	40.0
42	35.35492881486949	37.0	34.0	40.0	29.0	40.0
43	35.23614389910756	37.0	34.0	40.0	29.0	40.0
44	35.05400761907593	36.0	34.0	40.0	29.0	40.0
45	34.91647517968521	36.0	34.0	40.0	28.0	40.0
46	34.731842660307336	36.0	33.0	40.0	28.0	40.0
47	34.56463950610367	35.0	33.0	39.0	27.0	40.0
48	34.36364564150092	35.0	33.0	39.0	26.0	40.0
49	34.70729020812095	35.0	34.0	39.0	28.0	40.0
50	34.79233501293002	35.0	34.0	39.0	29.0	40.0
51	34.8302091272253	35.0	34.0	39.0	29.0	40.0
52	34.32052862413531	35.0	34.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	1.0
13	1.0
14	9.0
15	29.0
16	96.0
17	271.0
18	573.0
19	1008.0
20	2024.0
21	3307.0
22	5372.0
23	8348.0
24	11965.0
25	16309.0
26	21715.0
27	29534.0
28	38692.0
29	50934.0
30	67197.0
31	87384.0
32	116824.0
33	167140.0
34	360402.0
35	320675.0
36	341914.0
37	544353.0
38	945262.0
39	356720.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.6450320205874	0.3342136302901435	0.014865374027104151	0.005888975095352798
8	99.69923346127084	0.2607443778869557	0.023069916999755864	0.016952243842447617
9	99.53843013645842	0.3212064280164274	0.061662714954737796	0.0787007205704187
10	72.29577406003668	18.1317255097251	3.2568605130497974	6.315639917188431
11	43.54311044229633	25.84553961593591	15.054450149825819	15.55689979194194
12	37.96773756439995	14.218730256925118	23.10142015779023	24.712112020884707
13	27.18236554983874	15.780766607338578	26.623284550130897	30.413583292691783
14	25.21742038877527	16.48678611185279	31.39658473749179	26.89920876188015
15	28.269596136374943	17.832531270171884	28.729622287998325	25.168250305454848
16	34.474203144484	17.931614705514082	24.14348287709023	23.450699272911688
17	33.29878086780623	19.165555098794705	23.72505118548499	23.810612847914072
18	32.96616812394977	18.204680191488887	24.087251741106932	24.741899943454403
19	30.863089333465215	22.33834048681813	22.01770580395659	24.780864375760064
20	32.27369897960642	22.011502369026047	22.20446635880096	23.51033229256657
21	30.84465055222006	22.359123423198334	23.33712209789306	23.45910392668855
22	28.635112813895237	20.58376895549593	27.448398570408415	23.332719660200418
23	32.04900313373519	18.498528613843895	25.219964654714527	24.232503597706387
24	33.63854042609879	18.67282512431169	22.57401383966322	25.1146206099263
25	29.960789717277734	20.423937597446816	21.192162974812913	28.423109710462533
26	26.70495834550674	19.55605703958363	25.238146150639984	28.500838464269645
27	26.379463828828648	18.146533709236714	24.26863789149535	31.205364570439286
28	26.97039103366378	22.543225363072466	21.22912629907646	29.25725730418729
29	32.34450961703938	21.08533239262197	20.439231780340087	26.130926209998567
30	30.425675702717676	18.477717090205946	24.753735068160598	26.34287213891578
31	33.96609322533449	18.20676706130423	23.019088855486267	24.808050857875017
32	35.830411239137554	18.605302021519343	22.07402270171312	23.49026403762998
33	35.93138143349089	18.396586452727252	22.150236330859773	23.521795782922087
34	30.645425952999116	19.336849947199337	28.169426385238456	21.848297714563092
35	28.479540957250045	25.774929089307165	24.76871479121868	20.97681516222411
36	29.043567552547668	27.72738161873632	21.46054015051763	21.768510678198385
37	31.69723692718997	25.170765984136356	19.42609936587745	23.705897722796223
38	30.449631824707506	26.021522774610627	20.536371282155663	22.9924741185262
39	33.024686240552626	22.504804088663953	20.178287291648918	24.292222379134504
40	30.68293243515981	21.011548680383598	20.315363192533464	27.99015569192313
41	25.96963690180448	24.498622380049294	21.763393559062134	27.76834715908409
42	25.321649530511465	22.097292729517086	21.873340152347215	30.707717587624234
43	25.624731637117925	19.20146069452171	23.922017391344124	31.251790277016244
44	26.350504936733536	18.337782463546958	23.885511463204484	31.42620113651502
45	29.81079237589271	20.114509119621093	22.338712121168808	27.73598638331739
46	27.413836575795397	23.975675674130418	21.87388331024436	26.736604439829826
47	25.873469366752218	21.522488738049812	24.174242766423237	28.429799128774736
48	27.777037685438394	20.26965216740012	26.18192587781463	25.771384269346854
49	30.82426783744828	19.70662612612355	23.573224259604316	25.895881776823853
50	26.626143275905346	20.69022790333619	24.040054178570877	28.643574642187588
51	25.062906260666622	20.368764190000064	25.528992910931827	29.03933663840149
52	24.79181329547618	21.332812282915512	25.178313020180887	28.69706140142742
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	6.5
4	13.0
5	23.5
6	34.0
7	32.0
8	30.0
9	161.5
10	293.0
11	1919.0
12	3545.0
13	5100.0
14	6567.0
15	6479.0
16	6260.5
17	6042.0
18	6372.5
19	6703.0
20	6693.0
21	6683.0
22	7008.0
23	7333.0
24	7859.5
25	8386.0
26	9588.5
27	10791.0
28	13729.0
29	16667.0
30	17802.0
31	18937.0
32	22333.0
33	25729.0
34	32105.5
35	38482.0
36	40969.5
37	43457.0
38	47748.0
39	55168.5
40	58298.0
41	62512.5
42	66727.0
43	73596.5
44	80466.0
45	89363.0
46	98260.0
47	110073.0
48	121886.0
49	129711.5
50	137537.0
51	157937.5
52	178338.0
53	191576.5
54	204815.0
55	234930.5
56	265046.0
57	297169.0
58	329292.0
59	349279.5
60	369267.0
61	399892.0
62	430517.0
63	346430.5
64	240633.5
65	218923.0
66	174921.5
67	130920.0
68	118056.0
69	105192.0
70	91934.0
71	78676.0
72	64693.0
73	50710.0
74	40034.0
75	29358.0
76	21966.5
77	14575.0
78	10125.5
79	5676.0
80	3925.0
81	2174.0
82	1354.0
83	534.0
84	341.0
85	148.0
86	99.0
87	50.0
88	38.5
89	16.5
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3498062.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.569715639790542
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.62482110035839	5.119006659214203
2	9.979235748081546	1.510799538308195
3	4.314195487781472	0.9797169917091958
4	2.3223181351710003	0.703171516334925
5	1.2769974482846325	0.48332537781263973
6	0.8247475248058874	0.3745862542440998
7	0.5385412318008477	0.28536227895244576
8	0.3981286093912912	0.2410976288925853
9	0.33021154585682216	0.22496467528006184
>10	5.364574752557871	11.32503123709431
>50	4.108650445498084	22.348849121466756
>100	2.760451531866278	37.15519293481364
>500	0.0953309087580789	4.853303967859798
>1k	0.05538989560252015	7.602306836528713
>5k	0.0037680201090149767	1.6955402683989638
>10k+	0.0026376140763104835	5.097744713089491
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	73021	2.0874701477561004	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	39089	1.1174473179720656	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	15086	0.4312673703324869	No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	14433	0.4125998910253735	No Hit
TCGTAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	14183	0.4054530765892657	No Hit
TCGTAAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	11959	0.341875015365651	No Hit
TCGTAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	10954	0.3131448213324978	No Hit
TCGTAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	7957	0.22746880987243792	No Hit
TCGTAAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	6943	0.19848133051958483	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	6376	0.18227235537849246	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC	6208	0.17746969607742802	No Hit
TCGTAAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA	5674	0.16220410044190184	No Hit
TCGTAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	5582	0.15957407272941418	No Hit
TCGTAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	5541	0.15840199516189252	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	5058	0.14459434967133228	No Hit
TCGTAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	5055	0.144508587898099	No Hit
TCGTAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	5051	0.14439423886712127	No Hit
TCGTAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	4837	0.13827656570981303	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	4656	0.13310227205807101	No Hit
TCGTAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	4332	0.12384000054887535	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT	4233	0.12100986203217667	No Hit
TCGTAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	3856	0.11023246586252615	No Hit
TCGTAAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	3765	0.10763102540778294	No Hit
TCGTAAGGGGCCGGGCAGTGGTGGCACACGCCTTTAATCTCAGCACTTGAGA	3745	0.10705928025289432	No Hit
TCGTAAGGGGGCTGTGACAGCCACACACCCCAAGGCCTCCAAGATGAGCTAC	3695	0.10562991736567276	No Hit
TCGTAAGGGGGTTCTTCTGCGCTGCGCCAGGAGCTGCAAGCACAGGCATCCA	3563	0.10185639934340786	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTC	3500	0.1000554021055087	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.016952243842447617	0.0	0.0	0.0	0.0
7	0.01706659287342534	0.0	0.0	0.0	0.0
8	0.025528421165776936	0.0	0.0	0.0	0.0
9	0.05548786728194069	0.0	0.0	0.0	0.0
10	0.06772321359655718	0.0	0.0	0.0	0.0
11	0.09468099764955566	0.0	0.0	0.0	0.0
12	0.10620166252056139	0.0	0.0	0.0	0.0
13	0.11554969580299033	0.0	0.0	0.0	0.0
14	0.12484055456993044	0.0	0.0	0.0	0.0
15	0.1339313025326595	0.0	0.0	0.0	0.0
16	0.14365097016576608	0.0	0.0	0.0	0.0
17	0.15202703668488438	0.0	0.0	0.0	0.0
18	0.1606032140082137	0.0	0.0	0.0	0.0
19	0.17286714758057461	0.0	0.0	0.0	0.0
20	0.18318714762631422	0.0	0.0	0.0	0.0
21	0.1919062612383657	0.0	0.0	0.0	0.0
22	0.1997105826025954	0.0	0.0	0.0	0.0
23	0.2080294746062248	0.0	0.0	0.0	0.0
24	0.21752044417737593	0.0	0.0	0.0	0.0
25	0.22558205086130548	0.0	0.0	0.0	0.0
26	0.23447268801982354	0.0	0.0	0.0	0.0
27	0.2419053750333756	0.0	0.0	0.0	0.0
28	0.25165362992422663	0.0	0.0	0.0	0.0
29	0.2601726327320671	0.0	0.0	0.0	0.0
30	0.26869163553990755	0.0	0.0	0.0	0.0
31	0.27663889319285934	0.0	0.0	0.0	0.0
32	0.28555811760912186	0.0	0.0	0.0	0.0
33	0.293791247839518	0.0	0.0	0.0	0.0
34	0.3025675359670583	0.0	0.0	0.0	0.0
35	0.31405961358031964	0.0	0.0	0.0	0.0
36	0.3271239903695246	0.0	0.0	0.0	0.0
37	0.338358782663086	0.0	0.0	0.0	0.0
38	0.347906926749726	0.0	0.0	0.0	0.0
39	0.3569405001969662	0.0	0.0	0.0	0.0
40	0.3658311373554843	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGC	20	6.3132326E-4	46.0	23
TCGTTAC	20	6.3132326E-4	46.0	23
TTATACG	20	6.3132326E-4	46.0	31
TACGCTA	45	3.110472E-10	46.0	37
CGTAAGG	359475	0.0	44.49386	2
TCGTAAG	360475	0.0	44.492294	1
GTAAGGG	358360	0.0	44.44489	3
TAAGGGG	259685	0.0	44.421703	4
AAGGGGG	126720	0.0	44.24669	5
TAAGGGA	65315	0.0	44.16183	4
AGGGGGG	62730	0.0	43.811096	6
AAGGGAT	18665	0.0	43.732655	5
AAGGGGT	27085	0.0	43.52889	5
AAGGGGA	70295	0.0	43.42826	5
AAGGGGC	39235	0.0	43.250668	5
TAAGGGT	12190	0.0	43.150944	4
AGGGGGC	29345	0.0	43.123528	6
AGGGGAG	35585	0.0	43.000984	6
AGGGGGT	19815	0.0	42.958874	6
AAGGGAG	24105	0.0	42.80357	5
>>END_MODULE