SRR1527661_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527661_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3522743
Sequences flagged as poor quality	0
Sequence length	52
%GC	56

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

$Per base sequence content$

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	69175	1.9636686525244675	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	37210	1.0562791551924169	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	14254	0.4046278709516987	No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	13650	0.3874821410474735	No Hit
TCGTAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	13545	0.3845015091932622	No Hit
TCGTAAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	11179	0.31733793807836674	No Hit
TCGTAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	10390	0.2949406187167216	No Hit
TCGTAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	7541	0.21406614107245406	No Hit
TCGTAAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	6465	0.1835217613092979	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	6086	0.17276309966409698	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC	5875	0.1667734489856342	No Hit
TCGTAAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA	5373	0.1525231900255	No Hit
TCGTAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	5278	0.14982642787168976	No Hit
TCGTAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	5169	0.146732248137318	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	4995	0.14179291535033922	No Hit
TCGTAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4785	0.13583165164191655	No Hit
TCGTAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	4734	0.13438391616987103	No Hit
TCGTAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	4536	0.12876329610192966	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	4428	0.125697503337598	No Hit
TCGTAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	4321	0.12266009754330646	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT	4053	0.11505238957255752	No Hit
TCGTAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	3760	0.10673500735080589	No Hit
TCGTAAGGGGGCTGTGACAGCCACACACCCCAAGGCCTCCAAGATGAGCTAC	3580	0.10162535274358647	No Hit

Adapter Content

Adapter graph

Kmer Content

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCACG	50	1.6370905E-11	46.0	33
TCGTAAG	363435	0.0	44.427364	1
CGTAAGG	362475	0.0	44.42003	2
GTAAGGG	361410	0.0	44.3651	3
TAAGGGA	67085	0.0	44.27204	4
TAAGGGG	261185	0.0	44.25641	4
AAGGGGG	126755	0.0	44.05302	5
AAGGGAT	19370	0.0	44.052658	5
AGGGGGG	62280	0.0	43.58478	6
AAGGGGT	27810	0.0	43.36174	5
AAGGGGA	70275	0.0	43.293346	5
CGATGCG	245	0.0	43.183674	22
TAAGGGT	12070	0.0	43.14167	4
AGGGGGC	29230	0.0	43.112213	6
AAGGGGC	40095	0.0	43.06871	5
AAGGGAG	24305	0.0	42.85826	5
AGGGGGT	20475	0.0	42.8547	6
AGGGGAG	35395	0.0	42.72496	6
TAAGGGC	23045	0.0	42.706444	4
AGGGGAT	10665	0.0	42.139706	6

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content