##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527661_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3522743
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.109290118524115	33.0	31.0	34.0	31.0	34.0
2	32.55850455170871	34.0	31.0	34.0	31.0	34.0
3	32.846475317671484	34.0	31.0	34.0	31.0	34.0
4	35.510126341887556	35.0	35.0	37.0	35.0	37.0
5	35.97512733685086	37.0	35.0	37.0	35.0	37.0
6	36.238185981776134	37.0	37.0	37.0	35.0	37.0
7	36.369260828848425	37.0	36.0	37.0	35.0	37.0
8	36.55293020240193	37.0	37.0	37.0	35.0	37.0
9	38.64169852867496	39.0	39.0	39.0	38.0	39.0
10	38.012409647822736	39.0	38.0	39.0	37.0	39.0
11	37.6998353271868	39.0	38.0	39.0	35.0	39.0
12	36.86770053904017	39.0	35.0	39.0	33.0	39.0
13	36.53128967966156	39.0	35.0	39.0	33.0	39.0
14	37.324011714734795	40.0	35.0	40.0	33.0	40.0
15	37.47258798044592	40.0	36.0	40.0	33.0	40.0
16	37.58446443581039	40.0	36.0	40.0	33.0	40.0
17	37.424640684829974	40.0	36.0	40.0	33.0	40.0
18	37.456695819138666	40.0	35.0	40.0	33.0	40.0
19	37.38852536219645	40.0	35.0	40.0	33.0	40.0
20	37.37318646293527	40.0	35.0	40.0	33.0	40.0
21	37.4204096069455	40.0	35.0	40.0	33.0	40.0
22	37.32937231015717	40.0	35.0	40.0	33.0	40.0
23	37.375119615595004	40.0	35.0	40.0	33.0	40.0
24	37.38146580661717	40.0	35.0	40.0	33.0	40.0
25	37.33155810685026	40.0	35.0	40.0	33.0	40.0
26	37.16659375946528	39.0	35.0	40.0	33.0	40.0
27	37.076492948818576	39.0	35.0	40.0	33.0	40.0
28	36.956089331523756	39.0	35.0	40.0	32.0	40.0
29	36.898359602162294	39.0	35.0	40.0	32.0	40.0
30	36.694078733532365	39.0	35.0	40.0	32.0	40.0
31	36.59733111385077	39.0	35.0	40.0	31.0	40.0
32	36.549588204419116	38.0	35.0	40.0	31.0	40.0
33	36.3044113635312	38.0	35.0	40.0	31.0	40.0
34	36.00738884443174	38.0	35.0	40.0	30.0	40.0
35	35.99672755009377	38.0	34.0	40.0	30.0	40.0
36	35.95393703145532	38.0	35.0	40.0	30.0	40.0
37	36.01454690279705	38.0	35.0	40.0	30.0	40.0
38	35.625739657988106	38.0	34.0	40.0	30.0	40.0
39	35.70130094644997	38.0	34.0	40.0	30.0	40.0
40	35.528330054165174	37.0	34.0	40.0	29.0	40.0
41	35.11619808768338	37.0	34.0	40.0	28.0	40.0
42	35.186003634099905	37.0	34.0	40.0	29.0	40.0
43	35.186854959331406	36.0	34.0	40.0	29.0	40.0
44	35.00922832009034	36.0	34.0	40.0	28.0	40.0
45	34.8672957408474	36.0	34.0	40.0	28.0	40.0
46	34.51456719948063	35.0	33.0	40.0	26.0	40.0
47	34.264819772546566	35.0	33.0	39.0	26.0	40.0
48	34.22470415809499	35.0	33.0	39.0	26.0	40.0
49	34.17308954981956	35.0	33.0	39.0	26.0	40.0
50	34.03300240749893	35.0	33.0	39.0	26.0	40.0
51	34.41983022888698	35.0	33.0	39.0	27.0	40.0
52	34.123918208055485	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	15.0
15	38.0
16	114.0
17	292.0
18	628.0
19	1143.0
20	2099.0
21	3565.0
22	5806.0
23	8494.0
24	12471.0
25	17378.0
26	22970.0
27	30724.0
28	40800.0
29	53547.0
30	70612.0
31	92893.0
32	124280.0
33	181864.0
34	366876.0
35	323410.0
36	362857.0
37	567479.0
38	924513.0
39	307873.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.64138740748332	0.33434173313239146	0.016833473233784015	0.007437386150508284
8	99.70227745821934	0.2584633622151829	0.023589572103329706	0.015669607462139586
9	99.51637119142667	0.32301533208638833	0.07922803338194129	0.08138544310498949
10	72.21758726083623	18.182308502209786	3.2922356243415996	6.307868612612387
11	43.537436594154045	25.830070487685308	15.0203122964122	15.61218062174845
12	37.965273084071136	14.252217661066958	23.130299315050802	24.652209939811108
13	27.43086850218707	15.794680452136303	26.51939128116925	30.255059764507376
14	25.241466663903665	16.41947766271908	31.404533342341466	26.934522331035787
15	28.24299132806452	17.827386215798313	28.777234104219353	25.15238835191781
16	34.47966541981632	17.892080120519722	24.168098552747104	23.460155906916853
17	33.287866869652426	19.12586299937293	23.723246345248576	23.863023785726067
18	32.946683876740366	18.23749277196775	24.04004493089618	24.775778420395696
19	30.838383611861552	22.37287818044064	22.006061753582365	24.782676454115443
20	32.257334696286385	22.016195901886686	22.190662219753186	23.535807182073743
21	30.813885656716938	22.419887002827057	23.35132026378308	23.41490707667292
22	28.81865069350787	20.533799939422206	27.363193965611455	23.284355401458466
23	32.02089962282233	18.593351828390546	25.16873924666091	24.217009302126215
24	33.58025266106554	18.689697204706672	22.578570165351262	25.15147996887653
25	29.924578659300437	20.476344712061028	21.1692990377101	28.429777590928435
26	26.873461958479517	19.542981137142277	25.21526549055665	28.368291413821563
27	26.413507882919646	18.12059522934259	24.28173159381766	31.184165293920106
28	27.026694822756014	22.50924918451332	21.270044394382445	29.19401159834822
29	32.37017290219582	21.057198893021717	20.450966760845173	26.121661443937295
30	30.413544218241295	18.468591095064273	24.738449554792954	26.379415131901474
31	33.99893208218709	18.242914683245417	23.016694660950286	24.741458573617205
32	35.808033682843174	18.55718682855945	22.12298768317757	23.51179180541981
33	35.88839719502672	18.434299635255822	22.185722886966207	23.491580282751254
34	30.676577882632937	19.31364280618825	28.126037011499278	21.88374229967954
35	28.484223799465358	25.7556114652701	24.788467396003625	20.97169733926091
36	29.203947037862253	27.569396915982804	21.554623769034528	21.672032277120415
37	31.696833972844455	25.139131636909077	19.423897797824026	23.740136592422438
38	30.471765893793556	25.997440063041783	20.47620277721083	23.05459126595383
39	33.03465509689467	22.519496880697798	20.189494379805737	24.256353642601802
40	30.66908372254235	21.020948732280498	20.31167189885836	27.998295646318795
41	26.253235050073197	24.439421212390457	21.777858901429937	27.52948483610641
42	25.35271519949085	22.104450991741377	21.846157951346438	30.696675857421337
43	25.65725061408113	19.22569997300399	23.919599017016	31.19745039589888
44	26.34069530476677	18.36784573839193	23.85598381715612	31.43547513968518
45	29.7523549120671	20.113048269487727	22.410547689683863	27.72404912876131
46	27.664266169856845	23.948780822217234	21.81788452918649	26.56906847873944
47	26.092905443286668	21.614179632178672	24.112829122079017	28.180085802455647
48	27.781958547643132	20.20669688365004	26.211335882293994	25.800008686412834
49	30.86132028365396	19.64897808327204	23.523402076166214	25.966299556907785
50	26.649914569413664	20.71425590796717	23.98744387541186	28.648385647207302
51	25.043524321813997	20.320187989870394	25.62193722335124	29.014350464964377
52	24.857419346231048	21.288098507327955	25.17944113436603	28.675041012074963
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	6.0
4	12.0
5	23.0
6	34.0
7	28.5
8	23.0
9	164.5
10	306.0
11	1895.0
12	3484.0
13	5094.5
14	6592.5
15	6480.0
16	6264.5
17	6049.0
18	6420.5
19	6792.0
20	6745.5
21	6699.0
22	6846.0
23	6993.0
24	7847.5
25	8702.0
26	9541.5
27	10381.0
28	13452.5
29	16524.0
30	17820.5
31	19117.0
32	22460.0
33	25803.0
34	32009.5
35	38216.0
36	41186.0
37	44156.0
38	48361.0
39	55804.0
40	59042.0
41	62976.5
42	66911.0
43	74288.5
44	81666.0
45	90698.0
46	99730.0
47	111104.5
48	122479.0
49	130715.5
50	138952.0
51	159306.0
52	179660.0
53	193676.0
54	207692.0
55	236979.5
56	266267.0
57	298714.0
58	331161.0
59	350803.0
60	370445.0
61	400605.0
62	430765.0
63	348842.5
64	243012.0
65	219104.0
66	175894.5
67	132685.0
68	119406.0
69	106127.0
70	92843.0
71	79559.0
72	65705.0
73	51851.0
74	40552.5
75	29254.0
76	22015.5
77	14777.0
78	10285.5
79	5794.0
80	3946.0
81	2098.0
82	1312.0
83	526.0
84	332.5
85	139.0
86	92.5
87	46.0
88	42.0
89	22.5
90	7.0
91	6.5
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3522743.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.878147653546738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.19481846938021	5.293707014550468
2	10.314715112596755	1.6252169732261443
3	4.419285041839379	1.0444734024816333
4	2.2934180292564	0.7227154346315237
5	1.2796383779981453	0.5040590042507221
6	0.8320013509355618	0.3932777694372427
7	0.5484410724293057	0.3024489823507314
8	0.4134175598847151	0.26055716634726295
9	0.3326400630230063	0.23585287787823025
>10	5.578342156157826	12.224743576451894
>50	4.080189437658596	22.900024656476443
>100	2.564291348155435	35.71290418475729
>500	0.09229489941201538	4.918933237066647
>1k	0.05123308702054731	7.593372994149969
>5k	0.002636997126057582	1.2401577332213367
>10k+	0.002636997126057582	5.027554992722476
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	69175	1.9636686525244675	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	37210	1.0562791551924169	No Hit
TCGTAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	14254	0.4046278709516987	No Hit
TCGTAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	13650	0.3874821410474735	No Hit
TCGTAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	13545	0.3845015091932622	No Hit
TCGTAAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	11179	0.31733793807836674	No Hit
TCGTAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	10390	0.2949406187167216	No Hit
TCGTAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	7541	0.21406614107245406	No Hit
TCGTAAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	6465	0.1835217613092979	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	6086	0.17276309966409698	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC	5875	0.1667734489856342	No Hit
TCGTAAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA	5373	0.1525231900255	No Hit
TCGTAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	5278	0.14982642787168976	No Hit
TCGTAAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	5169	0.146732248137318	No Hit
TCGTAAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	4995	0.14179291535033922	No Hit
TCGTAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4785	0.13583165164191655	No Hit
TCGTAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	4734	0.13438391616987103	No Hit
TCGTAAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	4536	0.12876329610192966	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	4428	0.125697503337598	No Hit
TCGTAAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	4321	0.12266009754330646	No Hit
TCGTAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT	4053	0.11505238957255752	No Hit
TCGTAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	3760	0.10673500735080589	No Hit
TCGTAAGGGGGCTGTGACAGCCACACACCCCAAGGCCTCCAAGATGAGCTAC	3580	0.10162535274358647	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.015697994432179695	0.0	0.0	0.0	0.0
7	0.01589670322246045	0.0	0.0	0.0	0.0
8	0.024441181204532945	0.0	0.0	0.0	0.0
9	0.053935243076205105	0.0	0.0	0.0	0.0
10	0.06631196201369217	0.0	0.0	0.0	0.0
11	0.09149120443926792	0.0	0.0	0.0	0.0
12	0.10168212668366668	0.0	0.0	0.0	0.0
13	0.10985757405521776	0.0	0.0	0.0	0.0
14	0.11726657323568594	0.0	0.0	0.0	0.0
15	0.12544202060723703	0.0	0.0	0.0	0.0
16	0.13446907707999137	0.0	0.0	0.0	0.0
17	0.14358129446286602	0.0	0.0	0.0	0.0
18	0.15255157699554012	0.0	0.0	0.0	0.0
19	0.16543926139374912	0.0	0.0	0.0	0.0
20	0.17554502272802755	0.0	0.0	0.0	0.0
21	0.18394756585989952	0.0	0.0	0.0	0.0
22	0.19192430444116984	0.0	0.0	0.0	0.0
23	0.20061071727344287	0.0	0.0	0.0	0.0
24	0.20943906495591647	0.0	0.0	0.0	0.0
25	0.21764289929750766	0.0	0.0	0.0	0.0
26	0.2259602815192593	0.0	0.0	0.0	0.0
27	0.2335679894900082	0.0	0.0	0.0	0.0
28	0.24262343293280264	0.0	0.0	0.0	0.0
29	0.25119629788491527	0.0	0.0	0.0	0.0
30	0.2590594885860252	0.0	0.0	0.0	0.0
31	0.26652526170657354	0.0	0.0	0.0	0.0
32	0.27475748301820485	0.0	0.0	0.0	0.0
33	0.28185422552823186	0.0	0.0	0.0	0.0
34	0.2891496768285396	0.0	0.0	0.0	0.0
35	0.2997096296834597	0.0	0.0	0.0	0.0
36	0.3119444137707463	0.0	0.0	0.0	0.0
37	0.3230437190564285	0.0	0.0	0.0	0.0
38	0.3335752849413085	0.0	0.0	0.0	0.0
39	0.34345395051526606	0.0	0.0	0.0	0.0
40	0.3538719685199857	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCACG	50	1.6370905E-11	46.0	33
TCGTAAG	363435	0.0	44.427364	1
CGTAAGG	362475	0.0	44.42003	2
GTAAGGG	361410	0.0	44.3651	3
TAAGGGA	67085	0.0	44.27204	4
TAAGGGG	261185	0.0	44.25641	4
AAGGGGG	126755	0.0	44.05302	5
AAGGGAT	19370	0.0	44.052658	5
AGGGGGG	62280	0.0	43.58478	6
AAGGGGT	27810	0.0	43.36174	5
AAGGGGA	70275	0.0	43.293346	5
CGATGCG	245	0.0	43.183674	22
TAAGGGT	12070	0.0	43.14167	4
AGGGGGC	29230	0.0	43.112213	6
AAGGGGC	40095	0.0	43.06871	5
AAGGGAG	24305	0.0	42.85826	5
AGGGGGT	20475	0.0	42.8547	6
AGGGGAG	35395	0.0	42.72496	6
TAAGGGC	23045	0.0	42.706444	4
AGGGGAT	10665	0.0	42.139706	6
>>END_MODULE