FastQCFastQC Report
Fri 17 Jun 2016
SRR1527660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8837546
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1468061.6611624991824652No Hit
AATAAAGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA842050.9528097505800819Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA820440.928357261167297Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGA622790.7047092032109366No Hit
AATAAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA608850.6889355936591447Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA605710.6853825711345661Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590040.6676514045867484No Hit
AATAAAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA517850.5859658325965149Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA507540.5742996981288697No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA455270.5151543199888295No Hit
AATAAAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA384940.4355734046532827Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAA375200.4245522456120737No Hit
AATAAAGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA306800.3471551944397234Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA305510.34569551321147296No Hit
AATAAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA297680.33683558761674337Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAA280670.3175881630488826No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAA259990.294188001963441No Hit
AATAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA246270.2786633302955368Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAA239180.2706407412193385No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAA227150.257028365114026No Hit
AATAAAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA203350.2300978122207228Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA184020.20822522451368286Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGAACGCAGAGTGCAG183380.2075010415787369No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGG154050.17431309551316623No Hit
AATAAAGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA128450.14534577811532748Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA126780.14345611326945285No Hit
AATAAAGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAA125280.14175880951567324No Hit
AATAAAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA119340.13503748665070597No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAA116980.1323670620780927No Hit
AATAAAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA116100.131371310542542No Hit
AATAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110780.12535153989580364No Hit
AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAA109970.12443499586876265No Hit
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGAGAGTGCAGTGCTAATAAA106340.12032752078461599No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA101750.1151337712980504No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGCAGAGTGCAGTGCTAA101150.11445484979653855No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAA99810.11293859177649541No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAAA92410.10456522659118267No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAA88920.10061616652405543No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC207.859345E-444.043
AGGGATT49750.039.8874366
AAGGGAT238400.038.7768445
GGAAGCA288600.038.336118
AAGCAGT236400.038.1277510
CGATTTA700.037.71428719
AAGGGAC322350.037.6665085
GAAGCAG264200.037.62159
AGGGATA26850.037.1992576
GGGAAGC316300.037.121097
AGGGACG190600.037.028336
AGGGATC123550.037.002026
AGGGAAG406000.036.971926
AGGGGTG340100.036.884456
AGGGCGC174850.036.7400636
AAGGGGT697550.036.2319565
AGGGTAT67600.036.0266276
AGGGGCT151200.035.9246036
AGGGAAC375000.035.8864026
AGGGATG47450.035.608016
Produced by FastQC (version 0.11.5)