##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527660_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8837546
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.711154997099875	33.0	30.0	34.0	3.0	34.0
2	30.147765454346715	34.0	31.0	34.0	16.0	34.0
3	31.97956118135057	34.0	31.0	34.0	28.0	34.0
4	35.94495146050725	37.0	35.0	37.0	35.0	37.0
5	36.215393051419476	37.0	35.0	37.0	35.0	37.0
6	36.41174111003213	37.0	37.0	37.0	35.0	37.0
7	36.46466021223539	37.0	37.0	37.0	35.0	37.0
8	36.57830160091953	37.0	37.0	37.0	35.0	37.0
9	38.57571739937761	39.0	39.0	39.0	37.0	39.0
10	38.025004113132766	39.0	39.0	39.0	35.0	39.0
11	37.73999875078444	39.0	38.0	39.0	35.0	39.0
12	36.74578089890564	39.0	35.0	39.0	33.0	39.0
13	36.432334043862404	39.0	35.0	39.0	32.0	39.0
14	37.335024564511464	40.0	36.0	40.0	33.0	40.0
15	37.65963311534673	40.0	37.0	40.0	33.0	40.0
16	37.62056763268898	40.0	36.0	40.0	33.0	40.0
17	37.70168313692511	40.0	36.0	40.0	33.0	40.0
18	37.63822773878631	40.0	36.0	40.0	33.0	40.0
19	37.465484875552555	40.0	36.0	40.0	33.0	40.0
20	37.56088228564808	40.0	36.0	40.0	33.0	40.0
21	37.577316938435175	40.0	36.0	40.0	33.0	40.0
22	37.63303138676732	40.0	36.0	40.0	33.0	40.0
23	37.57398332070917	40.0	36.0	40.0	33.0	40.0
24	37.22560765171689	39.0	36.0	40.0	33.0	40.0
25	36.69907426790197	38.0	35.0	40.0	33.0	40.0
26	36.08909396341473	37.0	35.0	40.0	32.0	40.0
27	35.82702336146256	37.0	35.0	40.0	31.0	40.0
28	35.615790627850764	36.0	35.0	40.0	31.0	40.0
29	35.395445749306425	37.0	35.0	40.0	30.0	40.0
30	35.305735551475486	37.0	35.0	40.0	29.0	40.0
31	35.16234054114117	37.0	35.0	40.0	27.0	40.0
32	34.88392433827219	37.0	35.0	40.0	25.0	40.0
33	34.62480806323384	36.0	35.0	40.0	23.0	40.0
34	34.13832403248594	36.0	35.0	40.0	21.0	40.0
35	33.853508202390124	35.0	34.0	40.0	18.0	40.0
36	33.391650238652225	35.0	33.0	40.0	16.0	40.0
37	32.743040092804044	35.0	33.0	40.0	12.0	40.0
38	32.08683915195463	35.0	31.0	40.0	10.0	40.0
39	31.3262399992034	35.0	29.0	40.0	9.0	40.0
40	30.559407102378874	35.0	23.0	40.0	8.0	40.0
41	29.942913111852544	35.0	21.0	40.0	8.0	40.0
42	29.459684283397223	35.0	18.0	40.0	8.0	40.0
43	29.05717627947849	35.0	17.0	40.0	8.0	40.0
44	28.53701174511567	35.0	15.0	39.0	8.0	40.0
45	28.409933481534352	35.0	15.0	39.0	8.0	40.0
46	28.14848205599156	35.0	15.0	39.0	8.0	40.0
47	27.993988489564863	35.0	14.0	38.0	8.0	40.0
48	27.7763122251358	35.0	13.0	38.0	8.0	40.0
49	27.531205834741908	35.0	12.0	37.0	8.0	40.0
50	26.887226046687623	34.0	10.0	36.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	24.0
11	47.0
12	88.0
13	109.0
14	187.0
15	648.0
16	2062.0
17	5250.0
18	9934.0
19	16791.0
20	26401.0
21	38921.0
22	56161.0
23	84874.0
24	126001.0
25	176744.0
26	234511.0
27	314419.0
28	414725.0
29	494659.0
30	481632.0
31	373219.0
32	305972.0
33	331406.0
34	485952.0
35	607877.0
36	869479.0
37	1187835.0
38	1646182.0
39	545434.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.14867769853758	0.5702940612699497	0.1930400135965346	0.08798822659593511
8	97.69446178837428	1.5790582589329663	0.47665947085310784	0.24982048183964192
9	95.26270075425916	2.7517933145694515	1.217577820811343	0.7679281103600479
10	64.97603520253247	23.066131706697764	4.7041565611087055	7.253676529661061
11	48.16926554045659	30.232057632288424	10.805974871304771	10.792701955950216
12	33.84250560053662	37.373089769490306	14.208650229373628	14.575754400599441
13	22.403198806546524	45.289280531043346	15.273052044085542	17.03446861832459
14	21.38509943823772	51.42838294703077	12.882659960129203	14.303857654602306
15	24.130239322092354	51.05067628502301	11.803978163168825	13.015106229715807
16	22.340206206564584	52.455783539910286	15.045443610703696	10.158566642821434
17	25.26099439821869	52.478685825227956	11.364568852032	10.895750924521355
18	22.942567993422607	51.59036230193313	13.462968113546452	12.0041015910978
19	20.827942508022023	51.76011530802782	15.943690703278943	11.468251480671219
20	21.404923946081862	52.367354014338375	11.624788148203132	14.602933891376631
21	19.181274982896838	49.117039956567126	16.908336318702048	14.79334874183399
22	18.236193622075632	51.024990421549155	15.06134168919743	15.677474267177788
23	18.875420846465747	51.71440126025936	14.790802786203319	14.619375107071578
24	17.980251531364022	49.717319717487186	19.012325367245612	13.29010338390318
25	19.31378914463359	51.418165178433014	15.119977876211339	14.148067800722055
26	18.61780408271708	51.98481569431153	16.03111316195695	13.366267061014451
27	16.921507395831377	51.474798547017464	18.86280422189599	12.740889835255171
28	20.805266529871528	52.75240434392081	15.02161346600063	11.42071566020703
29	22.366457837956375	50.031683003403884	14.817167571178697	12.784691587461044
30	21.348980814357287	49.718587037623344	14.752092945258784	14.180339202760587
31	24.464053709027368	48.2964162223314	13.795288873178144	13.444241195463084
32	23.428890780313903	47.94953259649228	16.140510046567226	12.481066576626588
33	25.274538882173854	46.50875933205892	14.85311646468375	13.363585321083477
34	24.140570244273693	44.72024247455119	17.228640167756975	13.910547113418136
35	24.476907956122663	45.63859695892955	17.087401864725795	12.797093220221994
36	25.031892337533517	44.17545323102137	16.81196341156244	13.980691019882668
37	23.904905275740575	44.13390323512885	18.06539960301197	13.895791886118614
38	25.79854181239905	43.460164167745205	16.48274306012099	14.25855095973475
39	24.659345478937254	42.16326568484057	16.529034191165735	16.648354645056447
40	24.50473242232629	41.477351291863144	18.688140350273706	15.329775935536857
41	24.18833237190505	41.4426018263441	18.597764583064123	15.771301218686725
42	25.33133066577532	41.384293784722594	18.361669630913376	14.922705918588713
43	25.71115329979612	41.341499099410626	18.331265263004006	14.616082337789246
44	25.222816379117006	44.416232741532546	15.91473470123946	14.446216178110982
45	24.008949995847264	45.54143197670484	16.038207891647748	14.411410135800143
46	23.389105980325308	46.491175265169765	15.74900996272042	14.370708791784507
47	23.789828081234315	45.34651361362079	15.760653466471346	15.103004838673542
48	23.277559177626912	45.55000901834061	16.093211848628567	15.07921995540391
49	22.834687366832377	45.75268971725862	16.201047213785365	15.211575702123644
50	20.990295269750224	46.17961818812598	16.843329584932288	15.986756957191508
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2742.0
1	2399.5
2	2057.0
3	3901.5
4	5746.0
5	38895.5
6	72045.0
7	85087.5
8	98130.0
9	160091.0
10	222052.0
11	175720.5
12	129389.0
13	111147.0
14	92905.0
15	104043.0
16	115181.0
17	138428.0
18	161675.0
19	193590.0
20	225505.0
21	255251.5
22	284998.0
23	311230.5
24	337463.0
25	355543.5
26	373624.0
27	376145.0
28	378666.0
29	373134.0
30	367602.0
31	351843.0
32	336084.0
33	346680.0
34	357276.0
35	405527.5
36	453779.0
37	437473.0
38	421167.0
39	449254.0
40	477341.0
41	503975.5
42	530610.0
43	587079.0
44	643548.0
45	593851.0
46	544154.0
47	538007.5
48	531861.0
49	486426.5
50	440992.0
51	372278.5
52	303565.0
53	266022.0
54	228479.0
55	206756.5
56	185034.0
57	171335.0
58	157636.0
59	137525.0
60	117414.0
61	99673.5
62	81933.0
63	66740.0
64	51547.0
65	44265.0
66	36983.0
67	30779.0
68	24575.0
69	25129.0
70	25683.0
71	18543.0
72	11403.0
73	7558.0
74	3713.0
75	2684.5
76	1656.0
77	1220.0
78	784.0
79	550.5
80	317.0
81	219.5
82	122.0
83	89.5
84	57.0
85	44.0
86	31.0
87	26.5
88	22.0
89	11.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	8837546.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.393645806447854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.38161011576415	20.92370773637437
2	11.22844759713834	6.151762328645362
3	4.176575868560534	3.4323492008131367
4	2.0185286011490216	2.2117943020024375
5	1.1345094610603534	1.5539175170175683
6	0.7250977213276018	1.191784209186645
7	0.5351124604339808	1.0261076845421715
8	0.391018088218836	0.8569128810064938
9	0.3374973628332631	0.8320754896258165
>10	2.383017105695594	13.662621986955894
>50	0.4050389227584817	7.6825994854785336
>100	0.24528221290282554	12.668148113173828
>500	0.0210392285657417	3.9464517822392997
>1k	0.014291512104332727	7.33446531079777
>5k	0.0014668707437388608	2.806031936652554
>10k+	0.0014668707437388608	13.719270035488071
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	146806	1.6611624991824652	No Hit
AATAAAGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	84205	0.9528097505800819	Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	82044	0.928357261167297	Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGA	62279	0.7047092032109366	No Hit
AATAAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	60885	0.6889355936591447	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	60571	0.6853825711345661	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	59004	0.6676514045867484	No Hit
AATAAAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	51785	0.5859658325965149	Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	50754	0.5742996981288697	No Hit
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45527	0.5151543199888295	No Hit
AATAAAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	38494	0.4355734046532827	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAA	37520	0.4245522456120737	No Hit
AATAAAGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	30680	0.3471551944397234	Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30551	0.34569551321147296	No Hit
AATAAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	29768	0.33683558761674337	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAA	28067	0.3175881630488826	No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAA	25999	0.294188001963441	No Hit
AATAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	24627	0.2786633302955368	Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAA	23918	0.2706407412193385	No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAA	22715	0.257028365114026	No Hit
AATAAAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	20335	0.2300978122207228	Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA	18402	0.20822522451368286	Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGAACGCAGAGTGCAG	18338	0.2075010415787369	No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGG	15405	0.17431309551316623	No Hit
AATAAAGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	12845	0.14534577811532748	Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12678	0.14345611326945285	No Hit
AATAAAGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAA	12528	0.14175880951567324	No Hit
AATAAAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11934	0.13503748665070597	No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAA	11698	0.1323670620780927	No Hit
AATAAAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11610	0.131371310542542	No Hit
AATAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11078	0.12535153989580364	No Hit
AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAA	10997	0.12443499586876265	No Hit
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGAGAGTGCAGTGCTAATAAA	10634	0.12032752078461599	No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10175	0.1151337712980504	No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGCAGAGTGCAGTGCTAA	10115	0.11445484979653855	No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAA	9981	0.11293859177649541	No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAAAAAA	9241	0.10456522659118267	No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGTAAAAAAAAAAA	8892	0.10061616652405543	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.4710357377489181	0.0	0.0	0.0	0.0
7	0.477451545938205	0.0	0.0	0.0	0.0
8	0.9448889997291103	0.0	0.0	0.0	0.0
9	2.1327300587742344	0.0	0.0	0.0	0.0
10	3.2882431389890363	0.0	0.0	0.0	0.0
11	4.313346714121771	0.0	0.0	0.0	0.0
12	4.82619270100546	0.0	0.0	0.0	0.0
13	5.269924479035243	0.0	0.0	0.0	0.0
14	5.658697561517643	0.0	0.0	0.0	0.0
15	6.00366889179417	0.0	0.0	0.0	0.0
16	6.291644762018778	0.0	0.0	0.0	0.0
17	6.565114342827749	0.0	0.0	0.0	0.0
18	6.811902308627304	0.0	0.0	0.0	0.0
19	7.0559745884208125	0.0	0.0	0.0	1.1315358358530751E-5
20	7.2971614518328956	0.0	0.0	0.0	1.1315358358530751E-5
21	7.557029971894913	3.394607507559225E-5	0.0	0.0	2.2630716717061502E-5
22	7.843862990925309	3.394607507559225E-5	0.0	0.0	2.2630716717061502E-5
23	8.186005481612204	3.394607507559225E-5	0.0	0.0	2.2630716717061502E-5
24	8.519480407796463	3.394607507559225E-5	0.0	0.0	2.2630716717061502E-5
25	8.876219710765861	3.394607507559225E-5	0.0	0.0	2.2630716717061502E-5
26	9.243731234892582	3.394607507559225E-5	0.0	0.0	2.2630716717061502E-5
27	9.611627481203492	3.394607507559225E-5	0.0	0.0	2.2630716717061502E-5
28	9.974431816253064	3.394607507559225E-5	0.0	0.0	2.2630716717061502E-5
29	10.264863119241472	3.394607507559225E-5	0.0	0.0	2.2630716717061502E-5
30	10.559763988781501	3.394607507559225E-5	0.0	0.0	2.2630716717061502E-5
31	10.773454531382354	3.394607507559225E-5	0.0	0.0	2.2630716717061502E-5
32	10.981306349070206	3.394607507559225E-5	0.0	0.0	2.2630716717061502E-5
33	11.163540195434344	3.394607507559225E-5	0.0	0.0	2.2630716717061502E-5
34	11.335103658866387	3.394607507559225E-5	0.0	0.0	2.2630716717061502E-5
35	11.52847181785532	3.394607507559225E-5	0.0	0.0	2.2630716717061502E-5
36	11.682507791189998	3.394607507559225E-5	0.0	0.0	2.2630716717061502E-5
37	11.83151974541349	3.394607507559225E-5	0.0	0.0	2.2630716717061502E-5
38	11.976650531720004	3.394607507559225E-5	0.0	0.0	2.2630716717061502E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGAC	20	7.859345E-4	44.0	43
AGGGATT	4975	0.0	39.887436	6
AAGGGAT	23840	0.0	38.776844	5
GGAAGCA	28860	0.0	38.33611	8
AAGCAGT	23640	0.0	38.12775	10
CGATTTA	70	0.0	37.714287	19
AAGGGAC	32235	0.0	37.666508	5
GAAGCAG	26420	0.0	37.6215	9
AGGGATA	2685	0.0	37.199257	6
GGGAAGC	31630	0.0	37.12109	7
AGGGACG	19060	0.0	37.02833	6
AGGGATC	12355	0.0	37.00202	6
AGGGAAG	40600	0.0	36.97192	6
AGGGGTG	34010	0.0	36.88445	6
AGGGCGC	17485	0.0	36.740063	6
AAGGGGT	69755	0.0	36.231956	5
AGGGTAT	6760	0.0	36.026627	6
AGGGGCT	15120	0.0	35.924603	6
AGGGAAC	37500	0.0	35.886402	6
AGGGATG	4745	0.0	35.60801	6
>>END_MODULE