FastQCFastQC Report
Fri 17 Jun 2016
SRR1527659_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527659_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10544139
Sequences flagged as poor quality0
Sequence length52
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1612101.5289062482958542No Hit
AATAAAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA767330.727731301721269Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAG755750.7167488971835443No Hit
AATAAAGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA729280.6916449033913532Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA646380.6130230263466747No Hit
AATAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA559200.5303420222362395No Hit
AATAAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA559170.5303135704110122Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA521890.49495743559526295Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA515590.48898255229753707Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA509890.4835767055043565No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAA434180.4117737825724794No Hit
AATAAAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA332790.3156160972460625No Hit
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAA316450.3001193364389449No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAA302720.28709788442659945No Hit
AATAAAGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA294140.2789606624116014Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA291220.27619135142281415Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGACGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAA278390.2640234541672867No Hit
AATAAAGGGCGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAA260570.24712306998229064No Hit
AATAAAGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA241620.22915100038040087Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGAACGCAGAGTGCAGTG212530.20156221385169526No Hit
AATAAAGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA191240.18137090188207874Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGGAGTGCAGTGCTAATAAAGGGGAGTGCAGTGCTAATAAAGGGG160320.15204655401450987No Hit
AATAAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA155010.14701058094928376Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA145310.13781115745913441Illumina Single End Adapter 2 (97% over 34bp)
AATAAAGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAA145110.13762147862428598No Hit
AATAAAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135320.12833669965845482No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGAAAAAAAAAAAAAAA133900.1269899799310309No Hit
AATAAAGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA129960.12325330688451659Illumina Single End Adapter 2 (100% over 34bp)
AATAAAGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA129500.1228170455643652No Hit
AATAAAGGGAGAGTGCAGTGCTAATAAAGGGAGAGTGCAGTGCTAATAAAGG127460.12088232144891109No Hit
AATAAAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA126920.1203701885948203No Hit
AATAAAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA125560.1190803725178509No Hit
AATAAAGGGGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAAAAAAAA120750.11451859653974591No Hit
AATAAAGGGAACGCAGAGTGCAGTGCTAATAAAGGGGGGAAAAAAAAAAAAA117860.11177773737618595No Hit
AATAAAGGGGCAGAGTGCAGTGCTAATAAAGGGGCAGAGTGCAGTGCTAATA115220.10927397675618652No Hit
AATAAAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA111150.10541401246702078No Hit
AATAAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG108950.10332754528368793No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAA351.02063495E-746.00000441
AGGGATT62750.041.5282866
AAGCAGT332600.040.6545410
GGAAGCA401850.040.3165368
GAAGCAG365650.040.2696539
TACGCGT800.040.2543
AAGGGAT291750.039.440965
GGGAAGC442250.038.885477
AAGGGAC390350.038.593575
AGGGATA31850.037.9120866
AGGGGTG417800.037.863576
AGGGCGC224000.037.7857136
AAGGGGT855850.037.400365
AGGGACG225550.037.3119056
AGGGATC147100.036.931346
CGATCTA1000.036.80000315
AGGGAAC455550.036.548576
AGGGGCT185050.036.5414776
AGGGACT40500.036.459266
CGATACG950.036.31579236
Produced by FastQC (version 0.11.5)